scholarly journals Complete DNA Sequence of an IncM1 Plasmid Bearing the Novel qnrE1 Plasmid-Mediated Quinolone Resistance Variant and blaCTX-M-8 from Klebsiella pneumoniae Sequence Type 147

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Marcos P. V. Cunha ◽  
Yame M. Davies ◽  
Louise Cerdeira ◽  
Milena Dropa ◽  
Nilton Lincopan ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Dna Sequence ◽  
Sequence Type ◽  
Quinolone Resistance ◽  
The Novel

scholarly journals Cooccurrence of NDM-1, ESBL, RmtC, AAC(6′)-Ib, and QnrB in Clonally Related Klebsiella pneumoniae Isolates Together with Coexistence of CMY-4 and AAC(6′)-Ib in Enterobacter cloacae Isolates from Saudi Arabia

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Mohamed H. Al-Agamy ◽  
Taghrid S. El-Mahdy ◽  
Hesham H. Radwan ◽  
Laurent Poirel
Keyword(s):  
Escherichia Coli ◽  
Saudi Arabia ◽  
16S Rrna ◽  
Klebsiella Pneumoniae ◽  
High Resistance ◽  
Enterobacter Cloacae ◽  
Sequence Type ◽  
Quinolone Resistance ◽  
Synergy Test ◽  
16S Rrna Methylase

The aim of this study was to investigate the mechanisms responsible for resistance to antimicrobials in a collection of enterobacterial isolates recovered from two hospitals in Saudi Arabia. A total of six strains isolated from different patients showing high resistance to carbapenems was recovered in 2015 from two different hospitals, with four being Klebsiella pneumoniae and two Enterobacter cloacae. All isolates except one K. pneumoniae were resistant to tigecycline, but only one K. pneumoniae was resistant to colistin. All produced a carbapenemase according to the Carba NP test, and all were positive for the EDTA-disk synergy test for detection of MBL. Using PCR followed by sequencing, the four K. pneumoniae isolates produced the carbapenemase NDM-1, while the two E. cloacae isolates produced the carbapenemase VIM-1. Genotyping analysis by Multilocus Sequence Typing (MLST) showed that three out of the four K. pneumoniae isolates were clonally related. They had been recovered from the same hospital and belonged to Sequence Type (ST) ST152. In contrast, the fourth K. pneumoniae isolate belonged to ST572. Noticeably, the NDM-1-producing K. pneumoniae additionally produced an extended-spectrum ß-lactamase (ESBL) of the CTX-M type, together with OXA-1 and TEM-1. Surprisingly, the three clonally related isolates produced different CTX-M variants, namely, CTX-M-3, CTX-M-57, and CTX-M-82, and coproduced QnrB, which confers quinolone resistance, and the 16S rRNA methylase RmtC, which confers high resistance to all aminoglycosides. The AAC(6′)-Ib acetyltransferase was detected in both K. pneumoniae and E. cloacae. Mating-out assays using Escherichia coli as recipient were successful for all isolates. The blaNDM-1 gene was always identified on a 70-kb plasmid, whereas the blaVIM-1 gene was located on either a 60-kb or a 150-kb plasmid the two E. cloacae isolates, respectively. To the best of our knowledge, this is the first report of the coexistence of an MBL (NDM-1), an ESBL (CTX-M), a 16S rRNA methylase (RmtC), an acetyltransferase (AAC[6′]-Ib), and a quinolone resistance enzyme (QnrB) in K. pneumoniae isolates recovered from different patients during an outbreak in a Saudi Arabian hospital.

scholarly journals Complete Nucleotide Sequence of KPC-3-Encoding Plasmid pKpQIL in the Epidemic Klebsiella pneumoniae Sequence Type 258

2010 ◽  
Vol 54 (10) ◽  
pp. 4493-4496 ◽  
Author(s):  
Azita Leavitt ◽  
Inna Chmelnitsky ◽  
Yehuda Carmeli ◽  
Shiri Navon-Venezia
Keyword(s):  
Nucleotide Sequence ◽  
Klebsiella Pneumoniae ◽  
Dna Sequence ◽  
Complete Nucleotide Sequence ◽  
Sequence Type ◽  
Incompatibility Group ◽  
Carbapenem Resistant ◽  
Transmissible Plasmid ◽  
Clone Sequence

ABSTRACT We have determined the entire DNA sequence of plasmid pKpQIL, the bla KPC-3-carrying plasmid harbored by the carbapenem-resistant Klebsiella pneumoniae clone sequence type 258 (ST 258) in Israel. pKpQIL is a 113,637-bp, self-transmissible plasmid that belongs to the incompatibility group IncFII. It consists of a large backbone of a pKPN4-like plasmid and carries the bla KPC-3-containing Tn4401a transposon of a pNYC-like plasmid.

scholarly journals Outbreak of KPC-2-producing Klebsiella pneumoniae endowed with ceftazidime-avibactam resistance mediated through a VEB-1-mutant (VEB-25), Greece, September to October 2019

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Irene Galani ◽  
Ilias Karaiskos ◽  
Maria Souli ◽  
Vassiliki Papoutsaki ◽  
Lamprini Galani ◽  
...  
Keyword(s):  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
General Hospital ◽  
Intensive Care Units ◽  
Horizontal Transmission ◽  
Sequence Type ◽  
The Novel ◽  
Outbreak Strain ◽  
Extended Spectrum

From September to October 2019, seven patients colonised or infected with a ceftazidime-avibactam (CZA)-resistant Klebsiella pneumoniae carbapenemase (KPC)-2-producing K. pneumoniae were detected in two intensive care units of a Greek general hospital. The outbreak strain was sequence type (ST)147 and co-produced KPC-2 and the novel plasmid-borne Vietnamese extended-spectrum β-lactamase (VEB)-25 harbouring a K234R substitution associated with CZA resistance. Epidemiological investigations revealed that the resistance was probably acquired by horizontal transmission independently from previous CZA exposure.

A multi-institutional outbreak of New Delhi metallo-β-lactamase–producing Escherichia coli with subsequent acquisition of the Klebsiella pneumoniae carbapenemase gene

2020 ◽  
pp. 1-4
Author(s):  
Ester Solter ◽  
Jason C. Kwong ◽  
Aaron Walton ◽  
Norelle Sherry ◽  
Benjamin P. Howden ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Common Ancestor ◽  
Sequence Type ◽  
Second Phase ◽  
New Delhi ◽  
Genetic Sequencing ◽  
Klebsiella Pneumoniae Carbapenemase ◽  
Carbapenemase Gene

Abstract We characterized 57 isolates from a 2-phase clonal outbreak of New Delhi metallo-β-lactamase–producing Eschericha coli, involving 9 Israeli hospitals; all but 1 isolate belonged to sequence-type (ST) 410. Most isolates in the second phase harbored blaKPC-2 in addition to blaNDM-5. Genetic sequencing revealed most dual-carbapenemase–producing isolates to be monophyletically derived from a common ancestor.

Emergence and Recovery of Ceftazidime-avibactam Resistance in blaKPC-33-Harboring Klebsiella pneumoniae Sequence Type 11 Isolates in China

2020 ◽  
Vol 71 (Supplement_4) ◽  
pp. S436-S439
Author(s):  
Qingyu Shi ◽  
Dandan Yin ◽  
Renru Han ◽  
Yan Guo ◽  
Yonggui Zheng ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Infection Control ◽  
Polymyxin B ◽  
Sequence Type ◽  
First Report ◽  
Clinical Strains

Abstract This is the first report of ceftazidime–avibactam resistance caused by the blaKPC-33 mutation through the D179Y variant during the treatment of blaKPC-2-positive Klebsiella pneumoniae-related infections in China. The blaKPC-33-containing K. pneumoniae was susceptible to meropenem–vaborbactam, cefepime–zidebactam, tigecycline, and polymyxin B. The blaKPC-33 gene was located on a 77 551-bp transformable plasmid harboring qnrS1 and blaLAP-2. Detecting blaKPC-33-positive K. pneumoniae clinical strains is important for infection control.

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