scholarly journals Fast, and Cost-Effective Fourier Transform-Infrared (FT-IR) – Spectroscopy- Based For Bacterial Typing

2019 ◽  
Vol 12 (1) ◽  
pp. 130
Author(s):  
B. AlHarbi ◽  
T. Tran ◽  
T. Scanlon ◽  
H. Zowawi
2020 ◽  
Author(s):  
Huayan Yang ◽  
Fangling Wu ◽  
Fuxin Xu ◽  
Keqi Tang ◽  
Chuanfan Ding ◽  
...  

Abstract Fourier transform infrared (FT-IR) spectroscopy is a label-free and highly sensitive technique that provides complete information on the chemical composition of biological samples. The bacterial FT-IR signals are extremely specific and highly reproducible fingerprint-like patterns, making FT-IR an efficient tool for bacterial typing and identification. Due to the low cost and high flux, FT-IR has been widely used in hospital hygiene management for infection control, epidemiological studies, and routine bacterial determination of clinical laboratory values. However, the typing and identification accuracy could be affected by many factors, and the bacterial FT-IR data from different laboratories are usually not comparable. A standard protocol is required to improve the accuracy of FT-IR-based typing and identification. Here, we detail the principles and procedures of bacterial typing and identification based on FT-IR spectroscopy, including bacterial culture, sample preparation, instrument operation, spectra collection, spectra preprocessing, and mathematical data analysis. Without bacterial culture, a typical experiment generally takes <2 h.


2020 ◽  
Author(s):  
Huayan Yang ◽  
Fangling Wu ◽  
Fuxin Xu ◽  
Keqi Tang ◽  
Chuanfan Ding ◽  
...  

Abstract Fourier transform infrared (FT-IR) spectroscopy is a label-free and highly sensitive technique that provides complete information on the chemical composition of biological samples. The bacterial FT-IR signals are extremely specific and highly reproducible fingerprint-like patterns, making FT-IR an efficient tool for bacterial typing and identification. Due to the low cost and high flux, FT-IR has been widely used in hospital hygiene management for infection control, epidemiological studies, and routine bacterial determination of clinical laboratory values. However, the typing and identification accuracy could be affected by many factors, and the bacterial FT-IR data from different laboratories are usually not comparable. A standard protocol is required to improve the accuracy of FT-IR-based typing and identification. Here, we detail the principles and procedures of bacterial typing and identification based on FT-IR spectroscopy, including bacterial culture, sample preparation, instrument operation, spectra collection, spectra preprocessing, and mathematical data analysis. Without bacterial culture, a typical experiment generally takes <2 h.


2014 ◽  
Vol 6 (14) ◽  
pp. 5269-5273 ◽  
Author(s):  
Sana Jawaid ◽  
Farah N. Talpur ◽  
Hassan Imran Afridi ◽  
Shafi M. Nizamani ◽  
Abid A. Khaskheli ◽  
...  

A simple, cost-effective and environmentally friendly analytical method was developed for the quantification of melamine (MEL) in liquid milk and infant powder by using transmission Fourier transform infrared (FT-IR) spectroscopy.


2014 ◽  
Vol 151 ◽  
pp. 347-354 ◽  
Author(s):  
Yingying Meng ◽  
Changhong Yao ◽  
Song Xue ◽  
Haibo Yang

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