Antagonistic activity of Trichoderma harzianum and Trichoderma viride strains against some fusarial pathogens causing stalk rot disease of maize, in vitro

Tobacco production is a key line in the Cuban economy and generates the largest income in the agricultural sector. Within phytopathogens affecting this plant, Fusarium spp., constitutes a pest of interest, due to vascular involvements that cause deterioration of the commercial value of the leaf. Therefore, the research was carried out with the aim of determining the antagonistic activity in vitro of native strains of Trichoderma harzianum and T. viride on isolates of Fusarium oxysporum and F. phyllophylum, from Nicotiana tabacum L. in Granma province, Cuba. The work was carried out at the Laboratory of Agricultural Microbiology, University of Granma. Half dextrose potato agar was prepared to establish the dual crops of Trichoderma spp. vs. Fusarium spp. Once the treatments were established and the incubation time had elapsed, the percentage inhibition of mycelial growth, antagonistic capacity and mycoparasitic activity was determined. A fully randomized design with four replicas per treatment was used and a two factorial ANOVA and Tukey test (p≤0.05) were used for statistical processing. It was shown that the strains of Trichoderma spp., recorded intermediate values of phytopathogenic inhibition, the competitiveness of the antagonist was mostly located in class two of the Bell scale and it was found that penetration, winding, vacuolization, deformation and granulation constitute the main forms of mycoparasitism. Which points to this biocontrol method as an alternative to consider for the management of Fusarium spp., in tobacco agroecosystems.

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Antagonistic activity of endophytic bacteria isolated from weed plant against stem end rot pathogen of pitaya in Vietnam

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00362-0 ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

The Anh Luu ◽

Quyet Tien Phi ◽

Thi Thu Hang Nguyen ◽

Mai Van Dinh ◽

Bich Ngoc Pham ◽

...

Keyword(s):

Endophytic Bacteria ◽

Indole Acetic Acid ◽

Pathogenic Fungi ◽

Antagonistic Activity ◽

Biofilm Production ◽

Weed Plant ◽

Seedling Biomass ◽

Rot Disease ◽

Positive Results

Abstract Background Fungal stem end rot disease of pitaya caused by Alternaria alternata is one of the most destructive diseases in Binh Thuan province, Vietnam. This study aimed to assess the antagonistic effects of some endophytic bacteria isolated from the weed plant (Echinochloa colonum) against A. alternata. Results A total of 19 endophytic bacteria were isolated and 5 of them presented in vitro antagonistic activity against A. alternata. Of five, strain EC80 significantly inhibited the pathogenic growth with a mean inhibition diameter of 11.88 ± 0.08 mm, while the other four (C79, EC83, EC90, and EC97) showed a weak inhibition. Interestingly, the combination of EC79 and EC80 reduced more biomass of pathogenic fungi than the single one did. EC79 showed positive results for amylase, indole acetic acid (IAA), and biofilm production, whereas EC80 presented positive capabilities for IAA and biofilm production and a negative one for amylase production. In addition, the combined filtrate of EC79 and EC80 presented non-antifungal activity on biocontrol tests in vitro, indicating that bacteria cells played a role in defending against the pathogen. Moreover, both isolates EC79 and EC80 significantly increased seedling biomass than the control. Conclusions The results suggest that those two strains in combination had the potential to be used as a biocontrol agent against A. alternata. More studies should be done in the future to evaluate their efficiency under the field conditions.

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Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

Ukrainian Journal of Ecology ◽

10.15421/2018_191 ◽

2018 ◽

Vol 8 (1) ◽

pp. 88-91 ◽

Cited By ~ 1

Author(s):

M. Skaptsov ◽

S. Smirnov ◽

M. Kutsev ◽

O. Uvarova ◽

T. Sinitsyna ◽

...

Keyword(s):

Antifungal Activity ◽

Trichoderma Harzianum ◽

Its Region ◽

Botanical Garden ◽

Antagonistic Activity ◽

Antagonistic Effect ◽

Soil Samples ◽

Morphological Observation ◽

The South

Trichoderma isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as Trichoderma harzianum, T. asperellum, T. ghanense, and T. longibranchiatum. Antagonistic activity against Cladosporium sp. and Botrytis sp. was evaluated in vitro. All isolates showed antagonistic effect by competition against Cladosporium sp. T. asperellum and T. longibranchiatum showed antagonism against Botrytis sp. All isolates showed hyper sporulation on the sclerotia of Botrytis sp. (except the T. ghanense) and colonies of the Cladosporium sp. Our study provides new isolates that affect the Cladosporium sp. and Botrytis sp.

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Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0025 ◽

2017 ◽

Vol 57 (2) ◽

pp. 185-193 ◽

Cited By ~ 5

Author(s):

Zeinab Fotoohiyan ◽

Saeed Rezaee ◽

Gholam Hosein Shahidi Bonjar ◽

Amir Hossein Mohammadi ◽

Mohammad Moradi

Keyword(s):

Verticillium Dahliae ◽

Trichoderma Harzianum ◽

Pathogenic Fungus ◽

Antagonistic Activity ◽

Wilt Disease ◽

Dual Culture ◽

Volatile Metabolites ◽

Biocontrol Potential

Abstract Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an effective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in different locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. The results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae significantly. Among them, isolates Tr8 and Tr19 were the most effective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all effective isolates also inhibited the growth of V. dahliae in the culture medium significantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most effective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the five most effective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. The overall results of this study suggest that Trichoderma fungal antagonist may be an effective biocontrol agent for the control of Verticillium wilt of pistachio.

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Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen

Journal of Applied and Natural Science ◽

10.31018/jans.v9i4.1531 ◽

2017 ◽

Vol 9 (4) ◽

pp. 2327-2331 ◽

Cited By ~ 1

Author(s):

Balkishan Chaudhary ◽

Sanjeev Kumar ◽

Shiva Kant Kushwaha

Keyword(s):

Volatile Compounds ◽

Trichoderma Harzianum ◽

Mycelial Growth ◽

Biocontrol Agent ◽

Trichoderma Viride ◽

Control Agent ◽

Trichoderma Virens ◽

Trichoderma Species ◽

Fusarium Udum

Three biocontrol agent viz., Trichoderma viride, Trichoderma virens and Trichoderma harzianum were evaluated to test the antagonism against Fusarium udum under in vitro conditions. All the three biocontrol agents have the potential of parasitizing the growth of Fusarium udum in vitro. The rate of parasitism was found fastest in T. viride (61.12% over growth in 96 hrs) than T. virens and T. harzianum. The volatile compounds from Trichoderma viride suppressed the mycelial growth of Fusarium udum by 43.13% and found effective when compared to Tricho-derma virens and Trichoderma harzianum. Non-volatile compounds or culture filtrate from Trichoderma virens at 15% concentration shows complete mycelial inhibition of the test fungi. The antagonist T. virens was chosen to be the most promising bio-control agent for F. udum.

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Use of Trichoderma in Biological Control of Collar Rot of Soybean and Chickpea

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2020/v29i930221 ◽

2020 ◽

pp. 25-31

Author(s):

Mahbuba Kaniz Hasna ◽

Md. Abul Kashem ◽

Farid Ahmed

Keyword(s):

Seed Treatment ◽

Field Experiments ◽

Disease Incidence ◽

Sclerotium Rolfsii ◽

Antagonistic Activity ◽

Root Weight ◽

Dual Culture ◽

Collar Rot ◽

Rot Disease

An in vitro and field experiments for two consecutive years were conducted at Bangladesh Institute of Nuclear Agriculture, Mymensingh, aiming to investigate the efficacy of Trichoderma harzianum against Sclerotium rolfsii causing collar rot disease of soybean and chickpea. In in vitro the antagonistic activity of T. harzianum against S. rolfsii was observed through dual culture. In field experiment Trichoderma was applied as soil treatment and seed treatment. The percent inhibition of S. rolfsii induced by T. harzianum was found upto 78.9% in in vitro. The maximum reduction of collar rot disease incidence over control was 82.4% in soybean and 77.6% in chickpea which was recorded in the plot where T. harzianum was applied in the soil. The highest seed germination: 86.3% in soybean and 84.8% in chickpea, maximum fresh shoot weight: 94.5 g plant-1 in soybean, 62.5 g plant-1 in chickpea, maximum fresh root weight: 10.7 g plant-1 in soybean, 9.3 g plant-1 in chickpea and the highest yield: 2830 kg ha-1 in soybean, 1836 kg ha-1 in chickpea were obtained by the application of Trichoderma in soil. The study indicated that the tested isolate of T. harzianum had potential in controlling collar rot disease of soybean and chickpea. For the reduction of collar rot incidence application of T. harzianum in soil was found more effective than seed treatment.

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Bioefficacy of Trichoderma species against important fungal pathogens causing post-harvest rot in sweet potato (Ipomoea batatas (L.) Lam)

Journal of the Bangladesh Agricultural University ◽

10.3329/jbau.v17i4.44604 ◽

2019 ◽

Vol 17 (4) ◽

pp. 446-453

Author(s):

Victor Ohileobo DANIA

Keyword(s):

Ipomoea Batatas ◽

Antibiotic Production ◽

Trichoderma Viride ◽

Storage Period ◽

Economic Losses ◽

Trichoderma Species ◽

Post Harvest ◽

Rot Disease ◽

Tuber Rot

Tuber rot disease constitutes a serious threat to sweetpotato production worldwide, causing economic losses to farmers. This study evaluated an eco-friendly approach using four biological control agents, Trichoderma viride, T. harzianum, T. hamatum and T. pseudokoningii for the management of post-harvest tuber rot disease of sweetpotato. Field surveys for infected tuber samples were conducted four across major sweetpotato states in Nigeria. Rot severity in inoculated tubers was evaluated over a storage period of four months and Trichoderma species isolated from the rhizosphere were bioassayed for the production of metabolites to evaluate the mechanism of antibiotic production for the control of rot pathogens using Gas Chromatography-Mass Spectroscopy. A total of 24 metabolites were produced by the Trichoderma species and the abundances were species dependent. Trichoderma species significantly (p<0.05) inhibited rot in treated tubers at 4 months after storage. However, T. harzianum was most effective, reducing mycelia growth of the rot pathogens by 54.6-77.3% in vitro and 47.2-68.8% reduction of rot incidence in vivo. The efficacy of Trichoderma species used in this study recommends their use as alternative therapy to synthetic fungicides in the management of post-harvest rot in sweetpotato. J Bangladesh Agril Univ 17(4): 446–453, 2019

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Effects of biocontrol agents and plant products Macrophomina phaseolina and colchicine content in Gloriosa superba

Plant Protection Science ◽

10.17221/18/2011-pps ◽

2012 ◽

Vol 48 (No. 3) ◽

pp. 110-115 ◽

Cited By ~ 3

Author(s):

D. Alice ◽

S. Sundravadana

Keyword(s):

Mycelial Growth ◽

Foliar Application ◽

Trichoderma Viride ◽

Macrophomina Phaseolina ◽

Biocontrol Agents ◽

Gloriosa Superba ◽

Healthy Growth ◽

Rot Disease ◽

Tuber Rot

Gloriosa superba is a medicinal plant severely infested with soil-borne Macrophomina phaseolina (Tassi) Goid. Under in vitro conditions a commercial formulation of Trichoderma viride and Pseudomonas fluorescens inhibited the mycelial growth of M. phaseolina isolates. Among the oil cake, mahua cake at 10% completely inhibited the mycelial growth of the M. phaseolina isolates. Under field conditions both the soil and foliar application of biocontrol agents is attributed to the healthy growth of G. superba crops by controlling the tuber rot disease and ultimately boosting the colchicine content.

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