scholarly journals Effects of biocontrol agents and plant products Macrophomina phaseolina and colchicine content in Gloriosa superba

2012 ◽  
Vol 48 (No. 3) ◽  
pp. 110-115 ◽  
Author(s):  
D. Alice ◽  
S. Sundravadana
Keyword(s):  
Mycelial Growth ◽  
Foliar Application ◽  
Trichoderma Viride ◽  
Macrophomina Phaseolina ◽  
Biocontrol Agents ◽  
Gloriosa Superba ◽  
Healthy Growth ◽  
Rot Disease ◽  
Tuber Rot

Gloriosa superba is a medicinal plant severely infested with soil-borne Macrophomina phaseolina (Tassi) Goid. Under in vitro conditions a commercial formulation of Trichoderma viride and Pseudomonas fluorescens inhibited the mycelial growth of M. phaseolina isolates. Among the oil cake, mahua cake at 10% completely inhibited the mycelial growth of the M. phaseolina isolates. Under field conditions both the soil and foliar application of biocontrol agents is attributed to the healthy growth of G. superba crops by controlling the tuber rot disease and ultimately boosting the colchicine content.

scholarly journals Bioefficacy of Trichoderma species against important fungal pathogens causing post-harvest rot in sweet potato (Ipomoea batatas (L.) Lam)

2019 ◽  
Vol 17 (4) ◽  
pp. 446-453
Author(s):  
Victor Ohileobo DANIA
Keyword(s):  
Ipomoea Batatas ◽  
Antibiotic Production ◽  
Trichoderma Viride ◽  
Storage Period ◽  
Economic Losses ◽  
Trichoderma Species ◽  
Post Harvest ◽  
Rot Disease ◽  
Tuber Rot

Tuber rot disease constitutes a serious threat to sweetpotato production worldwide, causing economic losses to farmers.  This study evaluated an eco-friendly approach using four biological control agents, Trichoderma viride, T. harzianum, T. hamatum and T. pseudokoningii for the management of post-harvest tuber rot disease of sweetpotato. Field surveys for infected tuber samples were conducted four across major sweetpotato states in Nigeria. Rot severity in inoculated tubers was evaluated over a storage period of four months and Trichoderma species isolated from the rhizosphere were bioassayed for the production of metabolites to evaluate the mechanism of antibiotic production for the control of rot pathogens using Gas Chromatography-Mass Spectroscopy. A total of 24 metabolites were produced by the Trichoderma species and the abundances were species dependent. Trichoderma species significantly (p<0.05) inhibited rot in treated tubers at 4 months after storage. However, T. harzianum was most effective, reducing mycelia growth of the rot pathogens by 54.6-77.3% in vitro and 47.2-68.8% reduction of rot incidence in vivo. The efficacy of Trichoderma species used in this study recommends their use as alternative therapy to synthetic fungicides in the management of post-harvest rot in sweetpotato. J Bangladesh Agril Univ 17(4): 446–453, 2019

scholarly journals Management of wilt and dry root rot diseases of redgram [Cajanus cajan (L.) Millsp.] by using actinomycetes

2020 ◽  
Vol 15 (2) ◽  
pp. 96-100
Author(s):  
S. Malathi
Keyword(s):  
Root Rot ◽  
Cajanus Cajan ◽  
Mycelial Growth ◽  
Growth Parameters ◽  
Macrophomina Phaseolina ◽  
Antagonistic Activity ◽  
Fusarium Udum ◽  
Root Rot Disease ◽  
Rot Disease

Actinomycetes were tested for their antagonistic activity against Fusarium udum and Macrophomina phaseolina under in vitro condition. Among the tested isolates of Actinomycetes AC (5) reported highest 82.85 per cent reduction of mycelial growth of Fusarium udum and 85.13 per cent reduction of mycelial growth of Macrophomina phaseolina. In the field experiment, five treatments were tested for the management of wilt and dry root rot disease, T3- ST+ SA with Actinomycetes (AC 5) significantly recorded 71.92 and 70.38 per cent reduction of the wilt and dry root rot diseases, respectively. These biocontrol agents were used an alternative to the chemical fungicide for controlling the wilt and dry root rot incidence and enhanced the plant growth parameters and there by increased yield in redgram.

scholarly journals Halo Tolerance of Biocontrol Agents against Root Rot of Mung Bean (Vigna radiata (L.) Wilczek var. radiata) Caused by Macrophomina phaseolina (Tassi) Goid in Salt Affected Soils

2021 ◽  
pp. 316-326
Author(s):  
P. T. Sharavanan ◽  
V. K. Satya ◽  
M. Rajesh
Keyword(s):  
Field Condition ◽  
Root Rot ◽  
Trichoderma Viride ◽  
Soil Condition ◽  
Macrophomina Phaseolina ◽  
Biocontrol Agents ◽  
Soil Application ◽  
Salt Affected Soils ◽  
Ph Level

Root rot of mung bean [Vigna radiata (L.) Wilczek var. radiata] is major disease and claims huge yield loss if they occur in the field. The pathogen is basically soil borne and survivability may vary depends on soil condition. The fungicide chemicals are available to manage the disease; however, the biocontrol agents are nowadays available for the disease management and the microbial activity of the biocontrol agents is influenced by existing soil condition including soil pH. Hence, a study was conducted to find out the halo tolerance capacity of the biocontrol agents against root rot disease in salt affected soils under in vitro, in vivo and field condition. The root rot pathogen Macrophomina phaseolina was isolated from infected root. Efficacy of biocontrol agents against growth of M. phaseolina was assessed in vitro. The results revealed that TNAU strain of Bacillus subtilis reduced the mycelial growth of the M. phaseolina significantly when media supplemented with NaCl at 5% (1.4 cm), 7.5% (1.5 cm), 10% (1.6cm) and 12.5% (1.6 cm) and without NaCl (1.2 cm) and similar trend of reduction also expressed by BCA1 strain of B. subtilis, Pseudomonas fluorescens and Trichoderma viride under in vitro. The performance of the biocontrol agents against the pathogen is slightly reduced when media supplemented with NaCl. The reduction of mycelia weight of M.phaeolina was more in media added with TNAU strain of B.subtilis and the performance of TNAU strain of B.subtilis on reduction of mycelial weight of M.phaseolina is reduced when the broth added with NaCl at 5% (3.15g), 7.5% (3.25g), 10% (3.32g) and 12.5%(3.65g) level and which is followed by P. fluorescens, BCA 1 strain of B. subtilis and Trichoderma viride. Under pot culture conditions, the effect of talc formulated biocontrol agents and challenge inoculation with pathogen was assessed against root rot incidence. It was found that the soil application of TNAU strain of B.subtilis performed better in reducing the root rot incidence at pH of 7.0 (2.37%), 7.5 (4.50%), 8.0 (5.53%) and 8.7 (6.57%) and followed by BCA 1 of B.subtilis in all pH level. Among the biocontrol agents, TNAU strain of B.subtilis applied as seed as well as soil application expressed more population in the rhizosphere in all pH level. The biocontrol agents applied as soil application had more populations of the agents in the soil when compared to seed treatment. The halo tolerance performance of the biocontrol agents was also assessed under field condition in pH of 7.5 and 8.7 during 2019-20 and 2020-21. It was found that the minimum root rot incidence and maximum yield was observed from soil application of TNAU strain of B subtilis at 2.5 kg/ha but the effect is on par with soil application of BCA1 strain of B.subtilis at 2.5 kg/ha.

scholarly journals Efficacy of Trichoderma against Sclerotium rolfsii causing collar rot disease of lentil under in vitro conditions

2018 ◽  
Vol 10 (1) ◽  
pp. 307-312
Author(s):  
Shiva Kant Kushwaha ◽  
Sanjeev Kumar ◽  
Balkishan Chaudhary
Keyword(s):  
Culture Filtrate ◽  
Field Condition ◽  
Radial Growth ◽  
Mycelial Growth ◽  
Trichoderma Viride ◽  
Sclerotium Rolfsii ◽  
Control Agent ◽  
Collar Rot ◽  
Rot Disease

Three biocontrol agents viz., Trichoderma viride, T. virens and T. harzianum were evaluated to test the antagonism against Sclerotium rolfsii under in vitro conditions. All the three antagonists’ viz., T. viride, T. virens and T. harzianum have shown the potential of parasitizing the growth of Sclerotium rolfsii in vitro. The rate of inhibition was fastest in T. harzianum (63.60%) followed by T virens (51.5 %). Least inhibition was recorded in T. viride (50.85% ) after 72 hours of incubation. However, T. viride showed the highest (91.31%) reduction in sclerotia formation followed by T. harzianum (84.92%) and T. virens (84.29%) after 15 days of incubation. The volatile compounds from Trichoderma viride were found most effective in suppressing the mycelial growth (51.11%) and sclerotia production (95.90%) of the target pathogen. The culture filtrate from both T. harzianum and T. viride (15% concentration) was found very effective in inhibiting the radial growth (57.46 and 49.62%) and sclerotia formation (98.20 and 99.83%) of Sclerotium rolfsii. The antagonists such as T. harzianum and T. viride can be used as a bio-control agent against S. rolfsii under field condition.

Management of collar rot disease in chickpea by Trichoderma species

2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Maximum Growth ◽  
Pot Experiment ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Collar Rot ◽  
Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

scholarly journals Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 664
Author(s):  
Matías Olivera ◽  
Ninoska Delgado ◽  
Fabiola Cádiz ◽  
Natalia Riquelme ◽  
Iván Montenegro ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Inhibitory Effect ◽  
Biocontrol Agents ◽  
Environmental Cost ◽  
Antifungal Compounds ◽  
Table Grapes ◽  
Dual Cultures ◽  
Bunch Rot ◽  
Direct Bioautography

Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes.

scholarly journals Improving the Biocontrol Potential of Bacterial Antagonists with Salicylic Acid against Brown Rot Disease and Impact on Nectarine Fruits Quality

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 209
Author(s):  
Nadia Lyousfi ◽  
Rachid Lahlali ◽  
Chaimaa Letrib ◽  
Zineb Belabess ◽  
Rachida Ouaabou ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Disease Severity ◽  
Mycelial Growth ◽  
Brown Rot ◽  
Antagonistic Bacteria ◽  
Biological Treatments ◽  
Rot Disease ◽  
The Impact

The main objective of this study was to evaluate the ability of both antagonistic bacteria Bacillus amyloliquefaciens (SF14) and Alcaligenes faecalis (ACBC1) used in combination with salicylic acid (SA) to effectively control brown rot disease caused by Monilinia fructigena. Four concentrations of salicylic acid (0.5%, 2%, 3.5%, and 5%) were tested under in vitro and in vivo conditions. Furthermore, the impact of biological treatments on nectarine fruit parameters’ quality, in particular, weight loss, titratable acidity, and soluble solids content, was evaluated. Regardless of the bacterium, the results indicated that all combined treatments displayed a strong inhibitory effect on the mycelial growth of M. fructigena and disease severity. Interestingly, all SA concentrations significantly improved the biocontrol activity of each antagonist. The mycelial growth inhibition rate ranged from 9.79% to 88.02% with the highest reduction rate recorded for bacterial antagonists in combination with SA at both concentrations of 0.5% and 3.5%. The in vivo results confirmed the in vitro results with a disease severity varying from 0.00% to 51.91%. A significant biocontrol improvement was obtained with both antagonistic bacteria when used in combination with SA at concentrations of 0.5% and 2%. The lowest disease severity observed with ACBC1 compared with SF14 is likely due to a rapid adaptation and increase of antagonistic bacteria population in wounded sites. The impact of all biological treatments revealed moderate significant changes in the fruit quality parameters with weight loss for several treatments. These results suggest that the improved disease control of both antagonistic bacteria was more likely directly linked to both the inhibitory effects of SA on pathogen growth and induced fruit resistance.

scholarly journals In-vitro compatibility assay of indigenous Trichoderma and Pseudomonas species and their antagonistic activities against black root rot disease (Fusarium solani) of faba bean (Vicia faba L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Alemayehu Dugassa ◽  
Tesfaye Alemu ◽  
Yitbarek Woldehawariat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Pseudomonas Fluorescens ◽  
Faba Bean ◽  
Fusarium Solani ◽  
Root Rot ◽  
Mycelial Growth ◽  
Biocontrol Agents ◽  
Black Root Rot ◽  
Root Rot Disease ◽  
Rot Disease

Abstract Background Faba bean (Vicia faba L.) cultivation is highly challenged by faba bean black root rot disease (Fusarium solani) in high lands of Ethiopia. To ensure sustainable production of faba beans, searching for eco-friendly disease management options is necessary to curb the progress of the disease timely. The indigenous biocontrol agents that suit local environments may effectively strive with in-situ microorganisms and suppress local pathogen strains. This study aimed to screen antagonistic indigenous compatible Trichoderma and Pseudomonas strains against Fusarium solani. In the pathogenicity test, soil-filled pots were arranged in complete random block design and sown with health faba bean seeds. The effect of some fungicides was evaluated against Fusarium by food poisoning methods to compare with the biocontrol agents. The antagonistic efficacy of biocontrol agents and their compatibility was investigated on Potato dextrose agar medium. Results Fusarium solani AAUF51 strain caused an intense root rotting in faba bean plant. The effect of Mancozeb 80% WP at 300 ppm was comparable with Trichoderma and Pseudomonas strains against Fusarium. The mycelial growth of test the pathogen was significantly (P ≤ 0.05) reduced to 86.67 and 85.19% by Trichoderma harzianum AAUW1 and Trichoderma viridae AAUC22 strains in dual culture, respectively. The volatile metabolites of Pseudomonas aeruginosa AAUS31 (77.78%) found the most efficient in reducing mycelial growth of Fusarium followed by Pseudomonas fluorescens AAUPF62 (71.11%) strains. The cell-free culture filtrates of Pseudomonas fluorescens AAUPF62 and Pseudomonas aeruginosa AAUS31 were more efficient than the Trichoderma strain in reducing the growth of Fusarium isolates. There was no zone of inhibition recorded between Trichoderma harzianum AAUW1, Trichoderma viridae AAUC22, Pseudomonas aeruginosa AAUS31, and Pseudomonas fluorescens AAUPF62 strains, hence they were mutually compatible. Conclusions The compatible Trichoderma and Pseudomonas strains showed antagonistic potentiality that could be explored for faba bean protection against black root rot disease and might have a future dual application as biocontrol agents.

scholarly journals Evaluation of Bioagents and Biopesticides against Colletotrichum lindemuthianum and its Integrated Management in Common Bean

2010 ◽  
Vol 2 (3) ◽  
pp. 72-76 ◽  
Author(s):  
Bilal Ahmad PADDER ◽  
Prem Nath SHARMA ◽  
Renu KAPIL ◽  
Anju PATHANIA ◽  
Om Prakash SHARMA
Keyword(s):  
Mycelial Growth ◽  
Integrated Management ◽  
Trichoderma Viride ◽  
Significant Inhibition ◽  
Colletotrichum Lindemuthianum ◽  
Maximum Inhibition ◽  
Gliocladium Virens ◽  
Foliar Treatment

Three bioagents (Trichoderma viride, T. harzianum and Gliocladium virens) and five biopesticides (Achook, Neemgold, Wannis, Spictaf and Neemazal) were evaluated under in vitro and in vivo conditions against Colletotrichum lindemuthianum. All the three antagonistic fungi caused significant inhibition of mycelial growth, maximum being with T. viride (69.21%) followed by T. harzianum (64.20%). Among the biopesticides tested at four concentrations, Wanis applied @ 1000 ?l/ml caused maximum inhibition of 82.12 per cent followed by Spictaf (52.85%). T. viride and Wanis @ 1000 ?l/ml were most effective in reducing the seed borne infection. Integration of bioagents with Bavistin showed that disease can be effectively managed with seed dressing either with Bavistin or biopesticide followed by foliar treatment with fungicide or biopesticide.

scholarly journals In vitro antifungal potential of plant extracts against Fusarium oxysporum, Rhizoctonia solani and Macrophomina phaseolina.

2017 ◽  
Vol 6 (9) ◽  
pp. 1676 ◽  
Author(s):  
Ramaraju Cherkupally ◽  
Srinivasa Reddy Kota ◽  
Hindumathi Amballa ◽  
Bhumi Narasimha Reddy
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Aloe Vera ◽  
Datura Stramonium ◽  
Macrophomina Phaseolina ◽  
Calotropis Procera ◽  
Fungitoxic Effect

The antifungal activity of aqueous extracts of nine plants viz, Azadirachta indica, Parthenium hysterophorus, Momordica charantia, Allium sativum, Eucalyptus globules, Calotropis procera, Aloe vera, Beta vulgaris and Datura stramonium were assessed in vitro against Fusarium oxysporum f. sp. melongenae, Rhizoctonia solani and Macrophomina phaseolina, the soil borne phytopathogens. The assessment of fungitoxic effect was carried out by using three different concentrations i.e., 5, 10 and 20% against the test fungi, in terms of percentage of mycelial growth inhibition. The extract of A. sativum completely inhibited the mycelial growth of M. phaseolina at all the concentrations. The extracts of D. stramonium and E. globulus inhibited the mycelial growth of R. solani of 72%, and 70.7% respectively at 20% concentration, that of A. sativum, E. globulus and D. stramonium exhibited inhibition percentage of 63.3%, 61.8% and 61.1% respectively at 20% concentration on Fusarium oxysporum f. sp. melongenae. The application of plant extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly.

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