A new longitudinal variation in the structure of collagen fibrils and its relationship to locations of mechanical damage susceptibility

2020 ◽  
Vol 110 ◽  
pp. 103849 ◽  
Author(s):  
Samuel.J. Baldwin ◽  
Josh Sampson ◽  
Christopher.J. Peacock ◽  
Meghan L. Martin ◽  
Samuel P. Veres ◽  
...  
Keyword(s):  
Mechanical Damage ◽  
Collagen Fibrils ◽  
Longitudinal Variation ◽  
Damage Susceptibility

Assessments of the resistance of potato cultivars and breeder's clones to external mechanical damage

1988 ◽  
Vol 110 (3) ◽  
pp. 527-530 ◽  
Author(s):  
M. J. De Maine ◽  
P. D. S. Caligari
Keyword(s):  
Test Scores ◽  
Mechanical Damage ◽  
Rank Correlation ◽  
Potato Cultivars ◽  
Drop Test ◽  
Agricultural Practice ◽  
Damage Indices ◽  
Drop Tests ◽  
The Mean ◽  
Damage Susceptibility

SummaryTwenty potato cultivars and breeder's clones were subjected to tests of external damage susceptibility at two sites. At one site tubers were, as in usual agricultural practice, lifted by mechanical harvester, and damage indices were subsequently assigned to each clone. At the other site tubers were harvested by hand on two dates. Drop tests were carried out on this material after 1 week in store and also, in the case of the laterdug (maincrop) material, after 3 months.Ranks based on damage indices were approximately as expected from previous cultivar assessments carried out by other workers. The mean drop test scores showed varying degrees of rank correlation with the damage indices. The highest correlation was with ranks obtained from the drop test on early-harvested material. It was concluded that this was because damage had been inflicted in both cases before skin curing had occurred.

Managing Pipe Facilities With GIS: A User Perspective

2002 ◽  
Author(s):  
Gordon Gin ◽  
Stuart M. Thompson ◽  
David J. Stortini
Keyword(s):  
Spatial Data ◽  
Measurement Data ◽  
Mechanical Damage ◽  
Analysis Data ◽  
Document Management ◽  
Data Sets ◽  
Electronic Document Management ◽  
Inspection Data ◽  
Damage Susceptibility ◽  
Pipeline Failures

The Facilities Data Manager (FDM), XFIALS and DaVinci Projects use a unique combination of geo-spatial data base technology, electronic document management and CAD technology to graphically maintain pipeline facilities data. The same common ORACLE 8i™ database (called ORION) hosts cathodic protection measurements, in-line inspection measurements and pipeline risk analysis data. AM/FM/GIS is used to support pipeline risk applications. We will follow the processes involved with using pipeline facilities data and pipeline measurement data to quantitatively calculate the risk of pipeline failures. Pipeline measurement data sets in our database include: mechanical damage susceptibility, pipeline inspections, close interval surveys, hydrostatic test and in-line inspection data.

Effects of EDTA, Hyaluronidase and Collagenase on Epiphyseal Cartilage Matrix

1968 ◽  
Vol 26 ◽  
pp. 56-57
Author(s):  
H. Clarke Anderson ◽  
Priscilla R. Coulter
Keyword(s):  
Light And Electron Microscopy ◽  
Matrix Vesicles ◽  
Cartilage Matrix ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Dense Matrix ◽  
Type Iv ◽  
Bovine Testicular Hyaluronidase ◽  
The Matrix ◽  
Testicular Hyaluronidase

Epiphyseal cartilage matrix contains fibrils and particles of at least 5 different types: 1. Banded collagen fibrils, present throughout the matrix, but not seen in the lacunae. 2. Non-periodic fine fibrils <100Å in diameter (Fig. 1), which are most notable in the lacunae, and may represent immature collagen. 3. Electron dense matrix granules (Fig. 1) which are often attached to fine fibrils and collagen fibrils, and probably contain protein-polysaccharide although the possibility of a mineral content has not been excluded. 4. Matrix vesicles (Fig. 2) which show a selective distribution throughout the epiphysis, and may play a role in calcification. 5. Needle-like apatite crystals (Fig. 2).Blocks of formalin-fixed epiphysis from weanling mice were digested with the following agents in 0.1M phosphate buffer: a) 5% ethylenediaminetetraacetate (EDTA) at pH 8.3, b) 0.015% bovine testicular hyaluronidase (Sigma, type IV, 750 units/mg) at pH 5.5, and c) 0.1% collagenase (Worthington, chromatograhically pure, 200 units/mg) at pH 7.4. All digestions were carried out at 37°C overnight. Following digestion tissues were examined by light and electron microscopy to determine changes in the various fibrils and particles of the matrix.

Banded Structures in the Connective Tissue of Meningioma

1976 ◽  
Vol 34 ◽  
pp. 234-235
Author(s):  
C. N. Sun ◽  
H. J. White
Keyword(s):  
Connective Tissue ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Collagen Fibrils ◽  
Lead Citrate ◽  
Connective Tissues ◽  
Native Collagen ◽  
Routine Procedures

Previously, we have reported on extracellular cross-striated banded structures in human connective tissues of a variety of organs (1). Since then, more material has been examined and other techniques applied. Recently, we studied a fibrocytic meningioma of the falx. After the specimen was fixed in 4% buffered glutaraldehyde and post-fixed in 1% buffered osmium tetroxide, other routine procedures were followed for embedding in Epon 812. Sections were stained with uranyl acetate and lead citrate. There were numerous cross striated banded structures in aggregated bundle forms found in the connecfive tissue of the tumor. The banded material has a periodicity of about 450 Å and where it assumes a filamentous arrangement, appears to be about 800 Å in diameter. In comparison with the vicinal native collagen fibrils, the banded material Is sometimes about twice the diameter of native collagen.

Scanning electron microscopic study of collagen fibrillogenesis and extracellular compartmentalization in the chick embryo tendon

1986 ◽  
Vol 44 ◽  
pp. 208-209
Author(s):  
Grace C.H. Yang
Keyword(s):  
Chick Embryo ◽  
Extracellular Space ◽  
Fibroblast Cell ◽  
Collagen Fibrils ◽  
Electron Microscopic ◽  
Voltage Electron ◽  
Scanning Electron Microscopic Study ◽  
Microscopic Studies ◽  
And Function

The size and organization of collagen fibrils in the extracellular matrix is an important determinant of tissue structure and function. The synthesis and deposition of collagen involves multiple steps which begin within the cell and continue in the extracellular space. High-voltage electron microscopic studies of the chick embryo cornea and tendon suggested that the extracellular space is compartmentalized by the fibroblasts for the regulation of collagen fibril, bundle, and tissue specific macroaggregate formation. The purpose of this study is to gather direct evidence regarding the association of the fibroblast cell surface with newly formed collagen fibrils, and to define the role of the fibroblast in the control and the precise positioning of collagen fibrils, bundles, and macroaggregates during chick tendon development.

Platelet Adhesion to Native Collagens Involves Proteoglycans and May Be a Two-Step Process

1987 ◽  
Vol 58 (02) ◽  
pp. 786-789 ◽  
Author(s):  
O Behnke
Keyword(s):  
Electron Microscope ◽  
Platelet Adhesion ◽  
Close Contact ◽  
Blood Platelets ◽  
Collagen Fibrils ◽  
Platelet Membrane ◽  
Step Process ◽  
Rat Blood ◽  
Wide Gap ◽  
Rat Tail

SummaryAdhesion of rat blood platelets to native rat tail collagen fibrils was studied in the electron microscope under conditions that preserved collagen-associated proteoglycans (CAPG). The CAPG molecules were aligned in chain-like configurations that encircled the fibrils with a 65 nm period; they appeared to coat the fibrils completely and extended 60-100 nm away from the fibril. The initial platelet-fibril contact occurred between the platelet glycocalyx and the CAPG of the fibrils i.e. between two surfaces with net-negative charges. When close contact was established between the fibril surface proper and the platelet membrane, CAPG were not identified in the area of contact, and the collagen-platelet distance was reduced to a ~10-12 nm wide gap traversed by delicate links in register with fibril periodicities.

Use of the electro-separation method for improvement of the utility value of winter rapeseeds

2013 ◽  
Vol 27 (4) ◽  
pp. 419-424 ◽  
Author(s):  
S.J. Kovalyshyn ◽  
O.P. Shvets ◽  
S. Grundas ◽  
J. Tys
Keyword(s):  
Corona Discharge ◽  
Mechanical Damage ◽  
Edible Oil ◽  
Separation Method ◽  
Utility Value ◽  
X Ray ◽  
Winter Rapeseed ◽  
The Mean ◽  
Discharge Field ◽  
Prototype Apparatus

Abstract The paper presents the results of a study of the use of electro-separation methods for improvement of the utility value of 5 winter rapeseed cultivars. The process of electro-separation of rapeseed was conducted on a prototype apparatus built at the Laboratory of Application of Electro-technologies in Agriculture, Lviv National Agriculture University. The process facilitated separation of damaged, low quality seeds from the sowing material. The initial mean level of mechanically damaged seeds in the winter rapeseed cultivars studied varied within the range of 15.8-20.1%. Verification of the amount of seeds with mechanical damage was performed on X-ray images of seeds acquired by means of a digital X-ray apparatus. In the course of analysis of the X-ray images, it was noted that the mean level of mechanical damage to the seeds after the electro-separation was in the range of 2.1-3.8%. The application of the method of separation of rapeseeds in the corona discharge field yielded a significant reduction of the level of seeds with mechanical damage. The application of the method in practice may effectively contribute to improvement of the utility value of sowing material or seed material for production of edible oil.

Diseases of the kumara crop

2009 ◽  
Vol 62 ◽  
pp. 402-402
Author(s):  
S.L. Lewthwaite ◽  
P.J. Wright
Keyword(s):  
Fungal Pathogens ◽  
Ipomoea Batatas ◽  
Mechanical Damage ◽  
Soft Rot ◽  
Economic Loss ◽  
Root Surface ◽  
Black Rot ◽  
Rhizopus Stolonifer ◽  
Ceratocystis Fimbriata ◽  
Pink Rot

The predominant diseases of the commercial kumara (Ipomoea batatas) or sweetpotato crop are caused by fungal pathogens The field disease pink rot results from infection by the fungus Sclerotinia sclerotiorum Lesions form on vines but may spread down stems to the roots The widespread nature of this disease in sweetpotato appears peculiar to New Zealand Scurf is a disease caused by Monilochaetes infuscans which occurs in the field but may proliferate amongst stored roots The disease causes a superficial discolouration of the root surface which is mainly cosmetic but can also increase root water loss in storage Infection by Ceratocystis fimbriata produces a disease known as black rot The disease can be transmitted amongst plants at propagation but is particularly rampant amongst roots in storage This disease is readily transmitted and can cause severe economic loss Fusarium oxysporum causes surface rots in stored roots characterised by light to dark brown lesions that tend to be firm dry and superficial The lesions may be circular and centred on wounds caused by insects or mechanical damage at harvest Soft rot caused by Rhizopus stolonifer generally occurs in roots after they are washed and prepared for the market Fungal infection occurs through wounds or bruised tissue producing distinctive tufts of white fungal strands and black spores

Sign in / Sign up

Export Citation Format

Share Document