Evaluation of Sodium Dichloroisocyanurate as a root canal irrigation solution; Cl-concentration, pH, Cytotoxicity and Antimicrobial effect in vitro

2003 ◽  
Vol 28 (5) ◽  
pp. 425 ◽  
Author(s):  
Woo-Cheol Lee ◽  
Bong-Sun Kang ◽  
Cheol-Ho Kim ◽  
Ho-Hyun Son
Keyword(s):  
Root Canal ◽  
Antimicrobial Effect ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Sodium Dichloroisocyanurate ◽  
Irrigation Solution

The Evaluation of Anti-Microbial-Effect to Enterococcus Faecalis by Hypochlorous Acid Water

2021 ◽  
pp. 1-6
Author(s):  
Yoshishige Yamada
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Colony Formation ◽  
Hypochlorous Acid ◽  
Antimicrobial Effect ◽  
Endodontic Treatment ◽  
High Concentration ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Root Canal Irrigant

Periapical periodontitis is a major disease, and difficult to achieve successful treatment in some cases. Therefore, root canal irrigation is one of the most important processes to successful endodontic treatment. Sodium Hypochlorite (NaOCl) solution is the most commonly used root canal irrigate. However using NaOCl solution on endodontic treatment should be considered its problems such as allergy, serious tissue damage when inadvertently injected into the maxillary sinus or oral mucosa. Therefore, another root canal irrigant that is safe and has a high antimicrobial effect is desirable. In this study, Hypochlorous Acid (HClO) water was used to evaluate the microbicidal efficacy of the Enterococcus faecalis (E. faecalis) and considered the possibility to be a future root canal irrigant candidate. Original (200ppm), 10% diluted (20ppm), 25% diluted (50ppm) and 50% diluted (100ppm) HClO water were used in this study. Each experimental solution was mixed with low concentrations (5×107 CFU/ml) or high concentration (5×109 CFU/ml) of E. faecalis at 30 seconds, 1minute, 3 minutes, 5 minutes respectively. Then they were placed onto BHI agar plates and incubated for 48 h at 37 ºC, followed by counting the number of Colony formation. The results showed that in the condition of 5×107 CFU/ml E.faecalis, no colony formation was shown within 30 seconds by 50 % diluted HClO water. Whereas only original water was attained to inhibit colony formation completely within 30 seconds under the density of 5×109 CFU/ml E, faecalis. In conclusion, 200ppm HClO water has a capacity to anti-microbiological effect against E.faecalis same as NaOCl solution. Keywords: root canal irrigant, hypochlorous acid (HClO) water, Enterococcus faecalis (E. faecalis)

scholarly journals Hypochlorite solution for root canal irrigation that lacks a chlorinated odor

2017 ◽  
Vol 11 (02) ◽  
pp. 221-225
Author(s):  
La-ongthong Vajrabhaya ◽  
Vanida Sangalungkarn ◽  
Ratchapin Srisatjaluk ◽  
Suwanna Korsuwannawong ◽  
Chareerut Phruksaniyom
Keyword(s):  
Root Canal ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Commercial Products ◽  
Canal Irrigation ◽  
Hypochlorite Solution ◽  
Root Canal Irrigation ◽  
Chlorine Gas ◽  
Trichloroisocyanuric Acid

ABSTRACT Objectives: This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions. Materials and Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution. All required properties of the NaOCl irrigant were evaluated and compared with those of original 2.5% NaOCl solutions currently in use. Results: Our results revealed that a TCA 3.5% + 1/6 Buffer-1 solution passed the short-term stability test. Moreover, no odor of chlorine gas was detected by three independent observers. The hypochlorite ion content and pH were stable over an incubation period of 4 weeks. The new solution did not differ from commercial products in terms of the dissolution property on bovine pulpal tissue (P > 0.05). Moreover, the antibacterial effect of this solution on Enterococcus faecalis did not differ from that of the commercial products (P > 0.05). In addition, our biocompatibility analysis demonstrated no difference among the tested solutions (P > 0.05). Conclusions: According to the results of all properties tested, TCA 3.5% + 1/6 Buffer-1 could be considered an option for NaOCl irrigation with the benefit of no detectable chlorine odor.

Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants

2008 ◽  
Vol 9 (5) ◽  
pp. 68-75 ◽  
Author(s):  
Rafael Brandao Ferreira ◽  
Melissa Andreia Marchesan ◽  
Yara Teresinha Silva-Sousa ◽  
Manoel Sousa-Neto
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Distilled Water ◽  
Chlorhexidine Digluconate ◽  
Canal Irrigation ◽  
Root Canals ◽  
Root Canal Irrigation ◽  
Debris Removal ◽  
Group 2

Abstract Aim The aim of this in vitro study was to evaluate the cleaning capacity of different auxiliary chemical substances energized with ultrasound in radicular dentin using morphologic analysis. Methods and Materials Twenty-eight single-canal, mandibular incisors were prepared with the Hero 642 canal preparation system following the sequence: 25/12, 30/06, 35/02, and 40/02, 1 mm from the apex. The specimens were then divided into four groups of seven teeth. During biomechanical preparation the teeth were irrigated with 2 ml of distilled water between files. Each group of specimens (n=7) received a final irrigation with 100 ml of the following irrigants that were activated with ultrasound for 3 minutes: Group 1- distilled water; Group 2- 0.2% chlorhexidine digluconate (CHX); Group 3- 2.5% sodium hypochlorite (NaOCl); and Group 4-the filtrate obtained after combining 0.2% CHX and 2.5% NaOCl. The apical portions of the root canals from each group were then submitted to histological processing and analyzed using optical microscopy. Results Results showed statistical differences between the groups (p<0.01). Groups 1 and 2 (distilled water and 0.2% CHX respectively) were statistically similar in terms of a greater amount of debris, whereas 2.5% NaOCl and the filtrate were more efficient in removal of debris. Conclusion There is no additional benefit in terms of debris removal from root canal walls by irrigating with the filtrate obtained from the combination of NaOCl and CHX when compared to using NaOCl alone. Clinical Significance The findings of this study suggest the time and expense of preparing and using a combination of NaOCl and CHX is not warranted compared to using NaOCl alone for root canal irrigation. Citation Ferreira RB, Marchesan MA, Silva-Sousa YT, Sousa-Neto M. Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants. J Contemp Dent Pract 2008 July; (9)5:068-075.

scholarly journals An in vitro evaluation of the degree of pulp tissue dissolution through different root canal irrigation protocols

2018 ◽  
Vol 21 (2) ◽  
pp. 175 ◽  
Author(s):  
Alfredo Iandolo ◽  
Massimo Amato ◽  
Giuseppe Pantaleo ◽  
Dina Abtellatif ◽  
Andrea Blasi ◽  
...  
Keyword(s):  
Root Canal ◽  
Pulp Tissue ◽  
In Vitro Evaluation ◽  
Canal Irrigation ◽  
Root Canal Irrigation

SITOTOKSISITAS EKSTRAK DAUN MANGROVE DARUJU (Acanthus ilicifolius) SEBAGAI BAHAN IRIGASI SALURAN AKAR

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 44
Author(s):  
Twi Agnita Cevanti ◽  
Ratna Putri ◽  
Henu Sumekar
Keyword(s):  
Cell Viability ◽  
Root Canal ◽  
Fibroblast Cell ◽  
Chloroform Extract ◽  
Canal Irrigation ◽  
Whitney Test ◽  
Root Canal Irrigation ◽  
Mann Whitney Test ◽  
Acanthus Ilicifolius ◽  
Irrigation Solution

<p><strong><em>Background:</em></strong><em> The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. <strong>Purpose: </strong>The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. <strong>Methods: </strong>The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml,  6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is &gt; 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. <strong>Result: </strong>The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. <strong>Conclusions: </strong>Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.</em></p><p><strong><em>Keywords: </em></strong><em>Acanthus ilicifolius, root canal irrigation, fibroblast cell</em></p><p><em><strong>Correspondence</strong>: Twi Agnita Cevanti, Laboratorium Konservasi Fakultas Kedokteran Gigi Universitas Hang Tuah, Jl, Arif Rahman Hakim 150, Surabaya, Indonesia. Ph 031-5945864, fax: 031-5912191, e-mail address: [email protected]</em></p>

scholarly journals In Vitro Evaluation of Different Irrigation Protocols on Intracanal Smear Layer Removal in Teeth with or without Pre-Endodontic Proximal Wall Restoration

2020 ◽  
Vol 9 (10) ◽  
pp. 3325
Author(s):  
Naji Kharouf ◽  
Eugenio Pedullà ◽  
Giusy Rita Maria La Rosa ◽  
Frédéric Bukiet ◽  
Salvatore Sauro ◽  
...  
Keyword(s):  
Root Canal ◽  
Resin Composite ◽  
Smear Layer ◽  
Canal Irrigation ◽  
Layer Removal ◽  
Apical Level ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Sonic Activation

To investigate the influence of pre-endodontic coronal wall restoration on smear layer removal during different root canal irrigation strategies, single-root premolars were prepared with a mesio-occlusal cavity. Half were left untreated (G1), while the mesial walls of the remaining half were reconstructed using a resin composite (G2). The specimens were divided into control (ctrl) groups, which used the conventional needle irrigation method, and four experimental subgroups according to irrigation strategy: Sonic activation using the Endoactivator, sonic activation using the EQ-S, mechanical activation using the XP-Endo finisher, and ultrasonic activation using the EndoUltra. Smear layer removal was assessed through SEM and the results were statistically analyzed. At the coronal and middle thirds, no significant difference (p > 0.05) was detected for G1 and G2, except for the control subgroups (G1ctrl vs G2 ctrl) at the middle third. At the apical third, the smear layer removal was significantly greater for G2 than G1. In G1, both at the middle and apical level, EQ-S and EndoUltra showed greater smear layer removal (p < 0.05) compared to the others G1 subgroups. In G2, at the apical level, the EQ-S and EndoUltra were the most effective in smear layer removal. Pre-endodontic coronal wall restoration may improve the smear layer removal during root canal irrigation.

scholarly journals Improving the disinfection of lateral microcanals in endodontic dental treatment

2020 ◽  
Vol 24 (6) ◽  
pp. 364-368
Author(s):  
Olga Ye. Bekjanova ◽  
N. Sh. Abdulkhakova ◽  
M. M. Astanokulova
Keyword(s):  
Root Canal ◽  
Dental Treatment ◽  
Irrigation System ◽  
Effective Parameters ◽  
Dentinal Tubules ◽  
Canal Irrigation ◽  
In Vitro Experiment ◽  
Antiseptic Solution ◽  
Root Canal Irrigation

We studied the penetration depth of an antiseptic introduced in the dentin of the tooth by using various methods. An in vitro experiment was performed to determine effective parameters of ultraphonophoresis and electrophoresis, providing the maximum-diffusion antiseptic Decasan in the dentin of the tooth. Compared with the control, three physiotherapy sessions of the root canal significantly increased in-depth penetration in single-rooted teeth. The inclusion of Decasan in the endodontic treatment of patients with chronic apical periodontitis for drug processing the root canal irrigation system is effective because it promotes deep diffusion of antiseptic solution in dentinal tubules.

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