scholarly journals An in vitro evaluation of the degree of pulp tissue dissolution through different root canal irrigation protocols

2018 ◽  
Vol 21 (2) ◽  
pp. 175 ◽  
Author(s):  
Alfredo Iandolo ◽  
Massimo Amato ◽  
Giuseppe Pantaleo ◽  
Dina Abtellatif ◽  
Andrea Blasi ◽  
...  
Keyword(s):  
Root Canal ◽  
Pulp Tissue ◽  
In Vitro Evaluation ◽  
Canal Irrigation ◽  
Root Canal Irrigation

scholarly journals Hypochlorite solution for root canal irrigation that lacks a chlorinated odor

2017 ◽  
Vol 11 (02) ◽  
pp. 221-225
Author(s):  
La-ongthong Vajrabhaya ◽  
Vanida Sangalungkarn ◽  
Ratchapin Srisatjaluk ◽  
Suwanna Korsuwannawong ◽  
Chareerut Phruksaniyom
Keyword(s):  
Root Canal ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Commercial Products ◽  
Canal Irrigation ◽  
Hypochlorite Solution ◽  
Root Canal Irrigation ◽  
Chlorine Gas ◽  
Trichloroisocyanuric Acid

ABSTRACT Objectives: This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions. Materials and Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution. All required properties of the NaOCl irrigant were evaluated and compared with those of original 2.5% NaOCl solutions currently in use. Results: Our results revealed that a TCA 3.5% + 1/6 Buffer-1 solution passed the short-term stability test. Moreover, no odor of chlorine gas was detected by three independent observers. The hypochlorite ion content and pH were stable over an incubation period of 4 weeks. The new solution did not differ from commercial products in terms of the dissolution property on bovine pulpal tissue (P > 0.05). Moreover, the antibacterial effect of this solution on Enterococcus faecalis did not differ from that of the commercial products (P > 0.05). In addition, our biocompatibility analysis demonstrated no difference among the tested solutions (P > 0.05). Conclusions: According to the results of all properties tested, TCA 3.5% + 1/6 Buffer-1 could be considered an option for NaOCl irrigation with the benefit of no detectable chlorine odor.

scholarly journals Pulp tissue dissolution capacity of QMix 2in1 irrigation solution

2015 ◽  
Vol 09 (03) ◽  
pp. 423-427 ◽  
Author(s):  
Dilara Arslan ◽  
Mehmet Burak Guneser ◽  
Alper Kustarci ◽  
Kursat Er ◽  
Seyda Herguner Siso
Keyword(s):  
Saline Solution ◽  
In Vitro Study ◽  
Control Group ◽  
Pulp Tissue ◽  
Tissue Samples ◽  
Canal Irrigation ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Group 2

ABSTRACT Objective: The aim of this study was to evaluate the tissue dissolution efficacy of four root canal irrigation solutions (sodium hypochlorite [NaOCl], chlorhexidine gluconate [CHX], Octenidine [OCT], and QMix 2in1) on bovine pulp tissue. Materials and Methods: Fifty bovine pulp tissue samples, each weighing 6.55 mg, were prepared and randomly divided into four experimental groups and one control group (n = 10) according to the dissolution irrigants used: (1) 5.25% NaOCl group; (2) 2% CHX group; (3) OCT group; (4) QMix 2in1 group; and (5) control group (saline solution). These samples were then placed into special bovine dentin reservoir models and immersed for 1 h with each test solution (0.1 mL of each) at room temperature. The pulp samples were then blotted dry and weighed again. The percentage of weight loss was calculated. Statistically analyzed with one-way analysis of variance and post-hoc Tukey tests (P = 0.05). Results: Saline solution did not dissolve the bovine pulp tissue. All groups, except OCT, dissolved pulp samples more effectively than the control group (P < 0.05). The highest tissue dissolution was observed in 5.25% NaOCl group (P < 0.05). No statistically significant difference was found between the tissue-dissolving effect between QMix 2in1 and those of 2% CHX. Conclusions: Within the limitations of this in vitro study, NaOCl exhibited the best tissue-dissolving effect out of all solutions tested. CHX and QMix 2in1 were able to dissolve pulp tissue but less than NaOCl. OCT and saline solutions could not exhibit significantly tissue-dissolving effectiveness. This study shown that QMix 2in1 has little capacity to dissolve pulp tissue therefore used alone is not sufficient for this purpose.

Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants

2008 ◽  
Vol 9 (5) ◽  
pp. 68-75 ◽  
Author(s):  
Rafael Brandao Ferreira ◽  
Melissa Andreia Marchesan ◽  
Yara Teresinha Silva-Sousa ◽  
Manoel Sousa-Neto
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Distilled Water ◽  
Chlorhexidine Digluconate ◽  
Canal Irrigation ◽  
Root Canals ◽  
Root Canal Irrigation ◽  
Debris Removal ◽  
Group 2

Abstract Aim The aim of this in vitro study was to evaluate the cleaning capacity of different auxiliary chemical substances energized with ultrasound in radicular dentin using morphologic analysis. Methods and Materials Twenty-eight single-canal, mandibular incisors were prepared with the Hero 642 canal preparation system following the sequence: 25/12, 30/06, 35/02, and 40/02, 1 mm from the apex. The specimens were then divided into four groups of seven teeth. During biomechanical preparation the teeth were irrigated with 2 ml of distilled water between files. Each group of specimens (n=7) received a final irrigation with 100 ml of the following irrigants that were activated with ultrasound for 3 minutes: Group 1- distilled water; Group 2- 0.2% chlorhexidine digluconate (CHX); Group 3- 2.5% sodium hypochlorite (NaOCl); and Group 4-the filtrate obtained after combining 0.2% CHX and 2.5% NaOCl. The apical portions of the root canals from each group were then submitted to histological processing and analyzed using optical microscopy. Results Results showed statistical differences between the groups (p<0.01). Groups 1 and 2 (distilled water and 0.2% CHX respectively) were statistically similar in terms of a greater amount of debris, whereas 2.5% NaOCl and the filtrate were more efficient in removal of debris. Conclusion There is no additional benefit in terms of debris removal from root canal walls by irrigating with the filtrate obtained from the combination of NaOCl and CHX when compared to using NaOCl alone. Clinical Significance The findings of this study suggest the time and expense of preparing and using a combination of NaOCl and CHX is not warranted compared to using NaOCl alone for root canal irrigation. Citation Ferreira RB, Marchesan MA, Silva-Sousa YT, Sousa-Neto M. Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants. J Contemp Dent Pract 2008 July; (9)5:068-075.

scholarly journals In Vitro Evaluation of Different Irrigation Protocols on Intracanal Smear Layer Removal in Teeth with or without Pre-Endodontic Proximal Wall Restoration

2020 ◽  
Vol 9 (10) ◽  
pp. 3325
Author(s):  
Naji Kharouf ◽  
Eugenio Pedullà ◽  
Giusy Rita Maria La Rosa ◽  
Frédéric Bukiet ◽  
Salvatore Sauro ◽  
...  
Keyword(s):  
Root Canal ◽  
Resin Composite ◽  
Smear Layer ◽  
Canal Irrigation ◽  
Layer Removal ◽  
Apical Level ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Sonic Activation

To investigate the influence of pre-endodontic coronal wall restoration on smear layer removal during different root canal irrigation strategies, single-root premolars were prepared with a mesio-occlusal cavity. Half were left untreated (G1), while the mesial walls of the remaining half were reconstructed using a resin composite (G2). The specimens were divided into control (ctrl) groups, which used the conventional needle irrigation method, and four experimental subgroups according to irrigation strategy: Sonic activation using the Endoactivator, sonic activation using the EQ-S, mechanical activation using the XP-Endo finisher, and ultrasonic activation using the EndoUltra. Smear layer removal was assessed through SEM and the results were statistically analyzed. At the coronal and middle thirds, no significant difference (p > 0.05) was detected for G1 and G2, except for the control subgroups (G1ctrl vs G2 ctrl) at the middle third. At the apical third, the smear layer removal was significantly greater for G2 than G1. In G1, both at the middle and apical level, EQ-S and EndoUltra showed greater smear layer removal (p < 0.05) compared to the others G1 subgroups. In G2, at the apical level, the EQ-S and EndoUltra were the most effective in smear layer removal. Pre-endodontic coronal wall restoration may improve the smear layer removal during root canal irrigation.

scholarly journals Improving the disinfection of lateral microcanals in endodontic dental treatment

2020 ◽  
Vol 24 (6) ◽  
pp. 364-368
Author(s):  
Olga Ye. Bekjanova ◽  
N. Sh. Abdulkhakova ◽  
M. M. Astanokulova
Keyword(s):  
Root Canal ◽  
Dental Treatment ◽  
Irrigation System ◽  
Effective Parameters ◽  
Dentinal Tubules ◽  
Canal Irrigation ◽  
In Vitro Experiment ◽  
Antiseptic Solution ◽  
Root Canal Irrigation

We studied the penetration depth of an antiseptic introduced in the dentin of the tooth by using various methods. An in vitro experiment was performed to determine effective parameters of ultraphonophoresis and electrophoresis, providing the maximum-diffusion antiseptic Decasan in the dentin of the tooth. Compared with the control, three physiotherapy sessions of the root canal significantly increased in-depth penetration in single-rooted teeth. The inclusion of Decasan in the endodontic treatment of patients with chronic apical periodontitis for drug processing the root canal irrigation system is effective because it promotes deep diffusion of antiseptic solution in dentinal tubules.

scholarly journals Comparative Evaluation of the Efficacy of Novel Root Canal Irrigation Techniques on Reduction of Enterococcus faecalis Count: An In Vitro Study

2020 ◽  
Vol 21 (12) ◽  
pp. 1379-1383
Author(s):  
Sabari Murugesan ◽  
N Bharath Naga Reddy ◽  
Buggaveeti Pradeep Kumar ◽  
Purushotham Mohankumar ◽  
Arasappan Rajakumaran ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Comparative Evaluation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Canal Irrigation ◽  
Root Canal Irrigation

scholarly journals A Comparative Antimicrobial Analysis of Various Root Canal Irrigating Solutions on Endodontic Pathogens: An in vitro Study

2014 ◽  
Vol 15 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Abhinav Misuriya ◽  
Archana Bhardwaj ◽  
Sanjay Aggrawal ◽  
PS Pawan Kumar ◽  
Satyanarayana Gajjarepu
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Agar Plate ◽  
Zone Of Inhibition ◽  
Pulp Tissue ◽  
Agar Culture ◽  
Root Canal Irrigation ◽  
Micro Organisms

ABSTRACT Background and objectives Evolution in understanding the poly-microbial environment of both endodontic infections and that of failed root canal treatments has been debatable over the years. The present study was designed to compare and analyze the effect of various root canal irrigation solutions on certain endodontic pathogens in vitro. Materials and methods To analyze in vitro the zone of inhibition of the micro-organisms the following irrigating solutions were employed: a. Sodium hypochlorite (NaOCl) 5% b. Sodium hypochlorite (NaOCl) 3% c. Chlorhexidine 2% d. Chlorhexidine (CHX) 0.12% e. Doxycycline 0.01% f. Doxycycline 0.005% g. MTAD. An agar culture plate inoculated with four endodontic pathogens was used namely 1. Enterococcus faecalis (MTCC-439) 2. Candida albicans (MTCC-183) 3. Fusobacterium nucleatum (ATCC-25586) 4. Peptostreptococcus anaerobius (ATCC-27337) The inoculums were streaked out on the trypticase soy agar plate for discrete colonies with a wire loop using standard method. The inoculated plates carrying the antibiotic disks were incubated in an anaerobic jar in the following way–Anaerobic incubation–the following procedure was used to provide anaerobiosis with an increased concentration of carbon dioxide. By this method the zone of inhibition obtained by different irrigating solutions against different pathogens could be compared. Results MTAD showed maximum antibacterial activity. In case of C. albicans MTAD was less effective than 5% NaOCl, 3% NaOCl and 2% CHX, 0.12% CHX. However, it was more effective against E. faecalis, F. nuleatum and P. anaerobicus. In any case, antimicrobial activity is not the only prerequisite for an endodontic irrigant. E. faecalis strain used in this study showed resistance to doxycycline; also doxycycline was ineffective against C. albicans at 0.01% and 0.005% concentrations. Conclusion It was found that MTAD was more antimicrobial than 5% NaOCl for some of the test micro-organisms; however the ability of MTAD to dissolve pulp tissue is not comparable to 5% NaOCl. In addition, 5 and 3% NaOCl showed significant antimicrobial activity against all test micro-organisms. The best option for a primary endodontic irrigant therefore is 5% NaOCl. How to cite this article Misuriya A, Bhardwaj A, Bhardwaj A, Aggrawal S, Kumar PSP, Gajjarepu S. A Comparative Antimicrobial Analysis of Various Root Canal Irrigating Solutions on Endodontic Pathogens: An In Vitro Study. J Contemp Dent Pract 2014;15(2):153-160.

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