scholarly journals In Vitro Evaluation of Different Irrigation Protocols on Intracanal Smear Layer Removal in Teeth with or without Pre-Endodontic Proximal Wall Restoration

2020 ◽  
Vol 9 (10) ◽  
pp. 3325
Author(s):  
Naji Kharouf ◽  
Eugenio Pedullà ◽  
Giusy Rita Maria La Rosa ◽  
Frédéric Bukiet ◽  
Salvatore Sauro ◽  
...  
Keyword(s):  
Root Canal ◽  
Resin Composite ◽  
Smear Layer ◽  
Canal Irrigation ◽  
Layer Removal ◽  
Apical Level ◽  
Root Canal Irrigation ◽  
Significant Difference ◽  
Sonic Activation

To investigate the influence of pre-endodontic coronal wall restoration on smear layer removal during different root canal irrigation strategies, single-root premolars were prepared with a mesio-occlusal cavity. Half were left untreated (G1), while the mesial walls of the remaining half were reconstructed using a resin composite (G2). The specimens were divided into control (ctrl) groups, which used the conventional needle irrigation method, and four experimental subgroups according to irrigation strategy: Sonic activation using the Endoactivator, sonic activation using the EQ-S, mechanical activation using the XP-Endo finisher, and ultrasonic activation using the EndoUltra. Smear layer removal was assessed through SEM and the results were statistically analyzed. At the coronal and middle thirds, no significant difference (p > 0.05) was detected for G1 and G2, except for the control subgroups (G1ctrl vs G2 ctrl) at the middle third. At the apical third, the smear layer removal was significantly greater for G2 than G1. In G1, both at the middle and apical level, EQ-S and EndoUltra showed greater smear layer removal (p < 0.05) compared to the others G1 subgroups. In G2, at the apical level, the EQ-S and EndoUltra were the most effective in smear layer removal. Pre-endodontic coronal wall restoration may improve the smear layer removal during root canal irrigation.

scholarly journals Comparative Evaluation Of Antibacterial And Layer Removal Efficacy Of Two Different Herbal Irrigants – An In Vitro Study

2018 ◽  
Vol 1 (11) ◽  
pp. 350-354
Author(s):  
Arvind Kumar ◽  
A Sheerin Sarthaj ◽  
S Maria Antony
Keyword(s):  
Root Canal ◽  
Leaf Extract ◽  
Smear Layer ◽  
Antimicrobial Efficacy ◽  
Ocimum Sanctum ◽  
Layer Removal ◽  
Significant Difference ◽  
The Mean ◽  
Neem Leaf Extract

INTRODUCTION: Irrigation during root canal therapy is especially needed for teeth with complex internal anatomy. Biomechanical preparation of root canal produces "smear layer" which gets embedded with debris and microorganisms. The major side effects of these widely used chemical irrigants such as Sodium hypochlorite for antibacterial efficacy and EDTA for smear layer removal has led to the search of a more biocompatible irrigant.MATERIALS AND METHODS: Azadirachta indica(neem) and Ocimum sanctum(tulsi) extract were used in this study as herbal irrigants to evaluate the above mentioned properties by the q PCR method and SEM analysis.RESULTS: In antimicrobial efficacy, the Mean value of NaOcl showed higher antimicrobial efficacy followed by Neem leaf extract and the lower antimicrobial efficacy was recorded with Tulsi extract. Similarly, the mean smear layer removal by different irrigants at different root levels, EDTA showed the maximum smear layer removal, followed by Neem leaf extract and Tulsi extract showed the least smear layer removal. Hence Tulsi has poor smear layer removal efficacy and comparable antimicrobial efficacy when compared with Neem extractSTATISTICAL ANALYSIS: The collected data were analyzed by one-way analysis of variance to compare the mean of the groups. The post- hoc test (Tukey) was performed to find the interrelationship between different groups for significant difference (P < 0.05; confidence interval 95%).CONCLUSION: From this study, these two herbal irrigants are proved to be safe and effective. It can be concluded that neem leaf extract has a significant antimicrobial efficacy against E. faecalis and significant smear layer removal efficacy compared to 17% EDTA.

scholarly journals The Effect of Chitosan Nanoparticle as A Final Irrigation Solution on The Smear Layer Removal, Micro-hardness and Surface Roughness of Root Canal Dentin

2020 ◽  
Vol 14 (1) ◽  
pp. 19-26
Author(s):  
Diatri Nari Ratih ◽  
Raras Ajeng Enggardipta ◽  
Aqilla Tiara Kartikaningtyas
Keyword(s):  
Surface Roughness ◽  
Root Canal ◽  
Chitosan Nanoparticles ◽  
Lower Surface ◽  
Smear Layer ◽  
Micro Hardness ◽  
Canal Irrigation ◽  
Layer Removal ◽  
Root Canal Irrigation ◽  
Root Canal Dentin

Background: Root canal irrigation is one of the most important stages during root canal treatment. One of the requirements of root canal irrigation material is that it can eradicate the smear layer but does not alter the physical properties of the root canal dentin. Objective: To investigate the effect of chitosan nanoparticle as a final irrigation solution on the smear layer removal, micro-hardness and surface roughness of root canal dentin. Methods: Seventy-two premolars used in this study and divided by three evaluations, namely smear layer removal, micro-hardness and surface roughness. Each study used 24 teeth and was assigned randomly into three groups of eight teeth. Group-1, final irrigation with 17% EDTA; group-2, with 0.2% chitosan nanoparticles; group-3, with 2.5% NaOCl. Specimens were evaluated for smear layer removal, micro-hardness and surface roughness using a Scanning Electron Microscope, Vickers hardness tester and surface roughness measuring instrument, respectively. Data obtained from smear layer removal evaluation were statically analyzed using Kruskal-Wallis and Mann-Whitney U and data from micro-hardness and surface roughness were analyzed using one-way ANOVA and Tukey’s test. Results: Final irrigation using 0.2% chitosan nanoparticles produced similar smear layer removal than 17% EDTA (P>0.05), but it was greater than 2.5% NaOCl (P<0.05). Chitosan had higher micro-hardness and lower surface roughness than EDTA (P<0.05), but it was the same as 2.5% NaOCl (P>0.05). Conclusion: Final irrigation using 0.2% chitosan nanoparticles had the same effect on smear layer removal compared to 17% EDTA; however, 0.2% chitosan produced higher micro-hardness and lower surface roughness of root canal dentin than 17% EDTA.

scholarly journals The differences in root canal smear layer removal between 6,25% pineapple (Ananas comocus L. Merr.) peel extract and 17% Ethylene diamine tetra-acetic acid

2019 ◽  
Vol 52 (3) ◽  
pp. 122
Author(s):  
Nirawati Pribadi ◽  
Karlina Samadi ◽  
Meliavita N. K. Astuti ◽  
Hendy J. Kurniawan ◽  
Adelina K. Tandadjaja ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Root Canal ◽  
Smear Layer ◽  
Control Group ◽  
Canal Irrigation ◽  
Layer Removal ◽  
Root Canal Irrigation ◽  
Group I ◽  
Pineapple Peel ◽  
Peel Extract

Background: The smear layer is attached to dentine and occludes the orifice and, consequently, must be removed in order to improve the success of inroot canal treatment. The ideal irrigation material removes both the organic and inorganic smear layers. Ethylene diamine tetra-acetic acid (EDTA) is one of the most commonly used root canal irrigation materials, but removes only inorganic smear layer. To overcome this problem, Pineapple (Ananas comosus L. Merr.) peel extract, which contains saponins, bromelain, polyphenol and flavonoid, is used during root canal irrigation. Purpose: The study aimed to analyze the difference in smear layer removal between the use of 6.25% pineapple peel extract and 17% EDTA. Methods: 27 samples of mandibular premolar teeth with straight root canals were divided randomly into three groups (n = 9) and subsequently prepared using protaper. Irrigation was performed on the control group (aquadest), group I (17% EDTA) and group II (6.25% pineapple peel extract). The samples were dried, temporarily compressed and cut horizontally from the apical to the coronal. Samples were fixed with holder before the smear layer was observed through a scanning electron microscope (SEM). The resulting data was analyzed by means of an ANOVA test. Results: The highest score of root canal hygiene was recorded by group II, followed by group I and, finally, the control group. There were significant differences between the groups (p< 0.000). Conclusion: 6.25% pineapple peel extract produces a higher smear layer removal effect than 17%EDTA on the apical 1/3 of the root canal.

scholarly journals The Application of Nano Silver Argitos as a Final Root Canal Irrigation for the Treatment of Pulpitis and Apical Periodontitis. In Vitro Study

Nanomaterials ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 248
Author(s):  
Svetlana Razumova ◽  
Anzhela Brago ◽  
Dimitriy Serebrov ◽  
Haydar Barakat ◽  
Yuliya Kozlova ◽  
...  
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Ct Scanning ◽  
Endodontic Treatment ◽  
Smear Layer ◽  
Canal Irrigation ◽  
Nano Silver ◽  
Root Canal Irrigation ◽  
Silver Solution

Background: Endodontic treatment of various forms of pulpitis with variations of root canal system anatomy should be performed with high quality. The use of various antibacterial agents is aimed at maintaining the success of endodontic treatment. The aim of this study was to evaluate the penetration and fixation of the nano-silver solution on the dentinal surface during endodontic treatment. Materials and methods: the study was carried out on 70 extracted single-rooted teeth, randomly divided into two groups. In the teeth of the first group, the smear layer was removed after canal preparation with 17% EDTA solution; in the second group, the smear layer was not removed. In both groups, for the final treatment of the canal, a colloidal 1% solution of нанo серебра nanosilver was used. Samples were cut and prepared for analysis using micro-CT, scanning electron microscopy (SEM), X-ray microanalysis and energy dispersive spectrometry (elemental mapping). Results: in 100% of cases in groups of teeth with a preserved smear layer, the ability of a 1% colloidal solution of nanosilver with particles of 1–2 nm to be fixed on dentin with a removed and preserved smear layer and to leave a film on the dentinal surface was established. In the samples with removed smear layer, silver was found in 73.5% of cases. Conclusion: The nano-silver solution with a particle size of 1–2 nm proved its ability to penetrate the dentinal surfaces and create a final film covering the dentinal surface of the root canal before applying the sealer.

scholarly journals A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study

2013 ◽  
Vol 14 (6) ◽  
pp. 1028-1035
Author(s):  
Sangeetha Vallikanthan ◽  
K Balakoti Reddy ◽  
Shreemoy Dash ◽  
Sowmya Kallepalli ◽  
N Chakrapani ◽  
...  
Keyword(s):  
Root Canal ◽  
Comparative Evaluation ◽  
Group Iv ◽  
Smear Layer ◽  
Layer Removal ◽  
Group I ◽  
Rotary Instruments ◽  
Significant Difference ◽  
Sem Study ◽  
Cleaning Efficacy

ABSTRACT Objectives The present study was conducted to compare the cleaning efficacy (debris and smear layer removal) of hand and two NiTi rotary instrumentation systems (K3 and ProTaper). Materials and methods Sixty single rooted human maxillary anterior teeth decoronated at the cementoenamel junction were used. All the specimens were divided into four groups of 15 teeth each, group I—ProTaper rotary instrumentation done, group II—K3 rotary instrumentation done, group III—Stainless steel K-file instrumentation done, group IV—root canal irrigation without instrumentation. Root canal preparation was done in a crown down manner and 3% sodium hypochlorite was used as irrigant after each file followed by final rinse with 5 ml of 17% EDTA solution, then specimens were scanning electron microscopic (SEM) examination. Results Statistical analysis was done using one-way ANOVA followed by post hoc Tukey's HSD test. Group I showed highly statistical significant difference compared to other groups. There was no statistically significant difference considering smear layer at any levels among the groups with no smear layer formation in group IV. Conclusion ProTaper rotary instrumentation showed the maximum cleaning efficacy followed by K3 rotary instrumentation in the coronal, middle and apical thirds of the root canal. Clinical significance ProTaper rotary instruments are more efficient than hand and K3 rotary instruments during root canal treatment. How to cite this article Reddy KB, Dash S, Kallepalli S, Vallikanthan S, Chakrapani N, Kalepu V. A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study. J Contemp Dent Pract 2013;14(6):1028-1035.

scholarly journals In Vitro Evaluation of Antibacterial Properties and Smear Layer Removal/Sealer Penetration of a Novel Silver-Citrate Root Canal Irrigant

Materials ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 194 ◽  
Author(s):  
Riccardo Tonini ◽  
Massimo Giovarruscio ◽  
Fabio Gorni ◽  
Andrei Ionescu ◽  
Eugenio Brambilla ◽  
...  
Keyword(s):  
Root Canal ◽  
Ethylenediaminetetraacetic Acid ◽  
Antibacterial Properties ◽  
Phosphate Buffer Solution ◽  
Smear Layer ◽  
Tetrazolium Salt ◽  
Apical Portion ◽  
Layer Removal ◽  
Root Canal Irrigation ◽  
Group I

This study aimed at evaluating the efficacy of a novel silver-citrate root canal irrigation solution (BioAKT) on smear layer removal, sealer penetration after root canal instrumentation and antibacterial activity. Single-root teeth were endodontically treated, sealed with an epoxi-amine resin sealer and irrigated using: Group I: 5.25% sodium hypochlorite (NaOCl); Group II: silver-citrate solution (BioAKT); Group III: phosphate buffer solution (PBS); Group IV: 17% ethylenediaminetetraacetic acid (EDTA). Smear layer removal and silver deposition at the coronal, middle and apical portion of each canal were analyzed using scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDS). Sealer penetration into dentinal tubules at coronal, middle and apical portion was assessed through dye-assisted confocal microscopy (CSM). Both SEM and CSM micrographs were evaluated by two examiners (κ = 0.86), who were blind to the irrigation regimens; scores were given according to the degree of penetration of the sealer. Data analysis included Pearson’s x2 and Sidak’s multiple comparisons. Dentin discs were polished and sterilized. Enterococcus faecalis biofilms were grown using a continuous-flow bioreactor under anaerobic conditions for 72 h. Specimens were irrigated with the tested solutions, and bacterial viability was assessed using a tetrazolium salt assay (MTT). Statistical analysis included one-way ANOVA and Student’s post-hoc t-test (p < 0.05). BioAKT and EDTA were the most efficient solutions both in removing the smear layer and allowing sealer penetration. However, at the apical portion BioAKT performed significantly better compared to EDTA both in smear layer removal and sealer penetration (p < 0.05). BioAKT and NaOCl showed comparable antibacterial effect (p = 0.53). In conclusion, BioAKT represents a suitable smear layer removal agent, which allows for reliable sealer penetration at the apical portion of the root canal system and offers significant antibacterial properties.

scholarly journals Hypochlorite solution for root canal irrigation that lacks a chlorinated odor

2017 ◽  
Vol 11 (02) ◽  
pp. 221-225
Author(s):  
La-ongthong Vajrabhaya ◽  
Vanida Sangalungkarn ◽  
Ratchapin Srisatjaluk ◽  
Suwanna Korsuwannawong ◽  
Chareerut Phruksaniyom
Keyword(s):  
Root Canal ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Commercial Products ◽  
Canal Irrigation ◽  
Hypochlorite Solution ◽  
Root Canal Irrigation ◽  
Chlorine Gas ◽  
Trichloroisocyanuric Acid

ABSTRACT Objectives: This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions. Materials and Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution. All required properties of the NaOCl irrigant were evaluated and compared with those of original 2.5% NaOCl solutions currently in use. Results: Our results revealed that a TCA 3.5% + 1/6 Buffer-1 solution passed the short-term stability test. Moreover, no odor of chlorine gas was detected by three independent observers. The hypochlorite ion content and pH were stable over an incubation period of 4 weeks. The new solution did not differ from commercial products in terms of the dissolution property on bovine pulpal tissue (P > 0.05). Moreover, the antibacterial effect of this solution on Enterococcus faecalis did not differ from that of the commercial products (P > 0.05). In addition, our biocompatibility analysis demonstrated no difference among the tested solutions (P > 0.05). Conclusions: According to the results of all properties tested, TCA 3.5% + 1/6 Buffer-1 could be considered an option for NaOCl irrigation with the benefit of no detectable chlorine odor.

scholarly journals Evaluation of four final irrigation protocols for cleaning root canal walls

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Qiang Li ◽  
Qian Zhang ◽  
Xiaoying Zou ◽  
Lin Yue
Keyword(s):  
Root Canal ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Smear Layer ◽  
Confocal Laser ◽  
Bacterial Survival ◽  
Bacterial Inhibition ◽  
Layer Removal ◽  
Significant Difference ◽  
Canal Systems

Abstract The aim of this study was to compare the efficiency of four final irrigation protocols in smear layer removal and bacterial inhibition in root canal systems. Thirty roots inoculated with Enterococcus faecalis were prepared with ProTaper Universal files. The teeth were disinfected by conventional needle irrigation, sonic agitation using the EndoActivator device, passive ultrasonic irrigation, or an M3 Max file. Teeth with no root canal preparation served as blank controls for the establishment of the infection baseline. Teeth with preparation but no final irrigation served as a post-instrumentation baseline. After the final irrigation, the teeth were sectioned in half. One half of each tooth was examined by scanning electron microscopy (SEM) to assess smear layer removal using a five-point scale. The other half was examined by confocal laser scanning microscopy (CLSM) using the LIVE/DEAD BackLight bacterial viability kit to evaluate the depth of bacterial survival in dentinal tubules. SEM analysis revealed no significant difference in smear layer removal throughout the whole canal among the EA, PUI, and M3 Max groups (P > 0.05). CLSM revealed that PUI achieved the greatest bacterial inhibition depth in the coronal ((174.27 ± 31.63) μm), middle ((160.94 ± 37.77) μm), and apical ((119.53 ± 28.49) μm) thirds of the canal (all P < 0.05 vs. other groups). According to this comprehensive SEM and CLSM evaluation, PUI appears to have the best infection control ability in root canal systems.

scholarly journals A scanning electron microscopic evaluation of different root canal irrigation regimens

2006 ◽  
Vol 20 (3) ◽  
pp. 235-240 ◽  
Author(s):  
Chaves Medici Mônika ◽  
Izabel Cristina Fröner
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Ricinus Communis ◽  
Smear Layer ◽  
Electron Microscopic ◽  
Canal Irrigation ◽  
Root Canals ◽  
Root Canal Irrigation ◽  
Microscopic Evaluation ◽  
Scanning Electron

The purpose of this study was to assess the effectiveness of endodontic irrigants in removing the smear layer from instrumented root canal walls using Scanning Electron Microscopy (SEM). The endodontic irrigants used were: 1% sodium hypochlorite (NaOCl); 1% NaOCl mixed to 17% EDTAC; 2% chlorhexidine gel; and Ricinus communis gel. Photomicrographs of the middle and apical thirds were evaluated with the aid of the Fotoscore - v. 2.0 software. The results indicated that the mixture of sodium hypochlorite and EDTAC completely removed the smear layer from dentinal walls. The other endodontic irrigants were not as efficient in cleansing the root canals.

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