Investigation of the maximum quantum yield of PS II in Haematococcus pluvialis cell cultures during growth: Effects of chemical or high-intensity light treatment

The content of astaxanthin, including its mono- and diesters, and photosynthetic pigments, was studied in cells of H. pluvialis strain IBCE-H17 under combined prolonged action of several inducers of astaxanthin accumulation. The ineffectiveness of the simultaneous introduction of several inducers of astaxanthin accumulation in the culture medium during high-intensity light treatment was shown.

Download Full-text

Pigment Composition of Haematococcus pluvialis Green Alga under the Action of Several Inducers of Astaxanthin Accumulation

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-4-29-33 ◽

2020 ◽

Vol 36 (4) ◽

pp. 29-33

Author(s):

R.G. Goncharik ◽

E.A. Kulikov ◽

A.A. Selishcheva

Keyword(s):

Sodium Chloride ◽

High Intensity ◽

Sodium Acetate ◽

Haematococcus Pluvialis ◽

Basic Research ◽

Biotechnological Production ◽

Fundamental Research ◽

Simultaneous Decrease ◽

Valuable Compounds ◽

High Intensity Light

The content of astaxanthin, including its mono- and diesters, and photosynthetic pigments, has been analyzed in cells of H. pluvialis strain IBCE-H17 under the combined prolonged action of several inducers of astaxanthin accumulation. The effective induction of the astaxanthin accumulation, mainly in the form of monoesters of fatty acids, was shown after a 20-day cultivation under high-intensity light and with the addition of 1-2 g/l of sodium acetate to the culture medium. A simultaneous decrease in the content of chlorophylls and lutein in the H. pluvialis cells was observed under these conditions. The use of sodium acetate in combination with sodium chloride did not lead to noticeable changes in the content of astaxanthin compared with the use of sodium acetate alone. The obtained data can be helpful in the biotechnological production of Haematococcus biomass enriched with economically valuable compounds. astaxanthin, Haematococcus pluvialis, pigments, sodium acetate, sodium chloride, high-intensity light. This work was supported by Grant no. Б19РМ-010 of the Belarusian Republican Foundation for Fundamental Research and Grant no. 19-54-04003 of the Russian Foundation for Basic Research.

Download Full-text

Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.

Molecular and Cellular Biology ◽

10.1128/mcb.8.5.1985 ◽

1988 ◽

Vol 8 (5) ◽

pp. 1985-1992 ◽

Cited By ~ 164

Author(s):

R L Feinbaum ◽

F M Ausubel

Keyword(s):

Arabidopsis Thaliana ◽

High Intensity ◽

Chalcone Synthase ◽

State Level ◽

Fold Increase ◽

Single Copy ◽

Light Treatment ◽

Cross Hybridization ◽

Chalcone Synthase Gene ◽

High Intensity Light

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.

Download Full-text

Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene

Molecular and Cellular Biology ◽

10.1128/mcb.8.5.1985-1992.1988 ◽

1988 ◽

Vol 8 (5) ◽

pp. 1985-1992

Author(s):

R L Feinbaum ◽

F M Ausubel

Keyword(s):

Arabidopsis Thaliana ◽

High Intensity ◽

Chalcone Synthase ◽

State Level ◽

Fold Increase ◽

Single Copy ◽

Light Treatment ◽

Cross Hybridization ◽

Chalcone Synthase Gene ◽

High Intensity Light

We have cloned an Arabidopsis thaliana chalcone synthase (CHS) gene on the basis of cross-hybridization with a Petroselinum hortense CHS cDNA clone. The protein sequence deduced from the A. thaliana CHS DNA sequence is at least 85% homologous to the CHS sequences from P. hortense, Antirrhinum majus, and Petunia hybrida. Southern blot analysis indicated that CHS is a single-copy gene in A. thaliana. High-intensity light treatment of A. thaliana plants for 24 h caused a 50-fold increase in CHS enzyme activity and an accumulation of visibly detectable levels of anthocyanin pigments in the vegetative structures of these plants. A corresponding increase in the steady-state level of CHS mRNA was detected after high-intensity light treatment for the same period of time. The accumulation of CHS mRNA in response to high-intensity light was due, at least in part, to an increased rate of transcription of the CHS gene as demonstrated by nuclear runoff experiments.

Download Full-text

The influence of Rose Bengal photosensitizer on the productivity and pigment composition of Haematococcus pluvialis

Journal of the Belarusian State University Biology ◽

10.33581/2521-1722-2021-1-58-69 ◽

2021 ◽

pp. 58-69

Author(s):

Elizaveta I. Pechenkina ◽

Tatyana V. Samovich ◽

Nikolay V. Kozel

Keyword(s):

Rose Bengal ◽

High Intensity ◽

Haematococcus Pluvialis ◽

Dry Weight ◽

Cell Diameter ◽

Pigment Composition ◽

Combined Action ◽

High Intensity Light ◽

Primary Agent ◽

Stimulation Of

The stimulation of astaxanthin synthesis in Haematococcus pluvialis cells under high-intensity light in the combined action of Rose Bengal photosensitizer has been established. It was revealed that when Rose Bengal was used as an additional inducer of carotenogenesis in high-intensity light, the dry weight of the Haematococcus and the diameter of the cells in suspension increase compared to the action of only high-intensity light on algae cells. The observed increases in dry weight are significant and reach 40 % excess over control. We suggest that the increase in dry weight, Haematococcus cell diameter and astaxanthin yield when H. pluvialis photosensitizer is added to the incubation medium against the background of high-intensity light is not associated with increased stress (since the used concentrations of Rose Bengal are too small to induce photo-oxidative stress), but the signaling properties of the generated Rose Bengal singlet oxygen, which may be the primary agent in the transduction of a signal that triggers increased synthesis of astaxanthin in H. pluvialis cells.

Download Full-text

The 9-kDa Phosphoprotein Involved in Photoinhibition

Zeitschrift für Naturforschung C ◽

10.1515/znc-1988-5-615 ◽

1988 ◽

Vol 43 (5-6) ◽

pp. 413-417 ◽

Cited By ~ 27

Author(s):

Matthias Kuhn ◽

Andreas Thiel ◽

Peter Böger

Keyword(s):

Photosystem Ii ◽

Protein Pattern ◽

Light Treatment ◽

Short Term ◽

Ps Ii ◽

Molecular Weights ◽

Sds Page ◽

Herbicide Binding ◽

High Intensity Light ◽

Photosystem Ii Particles

Photosystem-II particles exhibit strong photoinhibition. Short-term illumination of photosystem- II particles with high-intensity light (5000 μE/m2 × s) leads to a typical change of the protein pattern on SDS-PAGE. Two proteins are mainly affected, namely the well-described 32-kDa herbicide-binding protein which probably is degraded [1] and, first published here, the 9-kDa phosphoprotein, whose function in the PS-II complex is still unknown. This protein is not degraded, but seems to be linked to other polypeptides of the PS-II complex. During light treatment new bands of 23, 41, 50 and 54 kDa appear in the protein pattern of SDS-PAGE. A monospecific antiserum was produced against the 9-kDa phosphoprotein to investigate its fate. After light treatment the antibodies reacted with new proteins of higher molecular weights, most pronounced with a 23-kDa and a 41-kDa peptide.

Download Full-text