Developmental variation during seed germination and biochemical responses of Brassica rapa exposed to various colored lights

2018 ◽  
Vol 179 ◽  
pp. 113-118 ◽  
Author(s):  
Tausif Nawaz ◽  
Nisar Ahmad ◽  
Shahid Ali ◽  
Maaz Khan ◽  
Hina Fazal ◽  
...  
Keyword(s):  
Seed Germination ◽  
Brassica Rapa ◽  
Developmental Variation ◽  
Biochemical Responses ◽  
Colored Lights

Seed germination and biochemical responses of two Elytrigia elongata accessions exposed to abiotic stresses

2021 ◽  
Author(s):  
Xiaoxia Tian ◽  
Peichun Mao ◽  
Mingli Zheng ◽  
Qingyi Meng ◽  
Lin Meng
Keyword(s):  
Seed Germination ◽  
Abiotic Stresses ◽  
Biochemical Responses

scholarly journals BrEXLB1, a Brassica rapa Expansin-Like B1 Gene Is Associated with Root Development, Drought Stress Response, and Seed Germination

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 404 ◽  
Author(s):  
Muthusamy Muthusamy ◽  
Joo Yeol Kim ◽  
Eun Kyung Yoon ◽  
Jin A. Kim ◽  
Soo In Lee
Keyword(s):  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Response ◽  
Seed Germination ◽  
Drought Tolerance ◽  
Root Growth ◽  
Brassica Rapa ◽  
Root Development ◽  
Clubroot Disease ◽  
Qrt Pcr

Expansins are structural proteins prevalent in cell walls, participate in cell growth and stress responses by interacting with internal and external signals perceived by the genetic networks of plants. Herein, we investigated the Brassica rapa expansin-like B1 (BrEXLB1) interaction with phytohormones (IAA, ABA, Ethephon, CK, GA3, SA, and JA), genes (Bra001852, Bra001958, and Bra003006), biotic (Turnip mosaic Virus (TuMV), Pectobacterium carotovorum, clubroot disease), and abiotic stress (salt, oxidative, osmotic, and drought) conditions by either cDNA microarray or qRT-PCR assays. In addition, we also unraveled the potential role of BrEXLB1 in root growth, drought stress response, and seed germination in transgenic Arabidopsis and B. rapa lines. The qRT-PCR results displayed that BrEXLB1 expression was differentially influenced by hormones, and biotic and abiotic stress conditions; upregulated by IAA, ABA, SA, ethylene, drought, salt, osmotic, and oxidative conditions; and downregulated by clubroot disease, P. carotovorum, and TuMV infections. Among the tissues, prominent expression was observed in roots indicating the possible role in root growth. The root phenotyping followed by confocal imaging of root tips in Arabidopsis lines showed that BrEXLB1 overexpression increases the size of the root elongation zone and induce primary root growth. Conversely, it reduced the seed germination rate. Further analyses with transgenic B. rapa lines overexpressing BrEXLB1 sense (OX) and antisense transcripts (OX-AS) confirmed that BrEXLB1 overexpression is positively associated with drought tolerance and photosynthesis during vegetative growth phases of B. rapa plants. Moreover, the altered expression of BrEXLB1 in transgenic lines differentially influenced the expression of predicted BrEXLB1 interacting genes like Bra001852 and Bra003006. Collectively, this study revealed that BrEXLB1 is associated with root development, drought tolerance, photosynthesis, and seed germination.

scholarly journals Doubled haploids of interspecific hybrids between Brassica napus and Brassica rapa for canola production with valuable breeding traits

OCL ◽  
2020 ◽  
Vol 27 ◽  
pp. 45
Author(s):  
Ainash Daurova ◽  
Dias Daurov ◽  
Dmitriy Volkov ◽  
Kuanysh Zhapar ◽  
Daniyar Raimbek ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Germination ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Interspecific Hybrids ◽  
Nutritional Value ◽  
Doubled Haploids ◽  
Doubled Haploid Plants ◽  
Haploid Plants

Doubled haploids (DH) were obtained from two interspecific hybrids between Brassica napus and Brassica rapa. Seeds of doubled haploid plants differed in colour and size. The hybridity of the obtained doubled haploid is shown using genomic in situ hybridization (GISH) analysis. Evaluation of drought tolerance during seed germination on PEG-6000 showed the advantage of doubled haploid plants of interspecific hybrids over the parent cultivars. The oil from seeds of doubled haploid plants showed good nutritional value.

scholarly journals Cold Stress-induced Seed Germination and Biosynthesis of Polyphenolics Content in Medicinally Important Brassica rapa

2021 ◽  
pp. 100185
Author(s):  
Muhammad Ilyas ◽  
Wahid Ali Khan ◽  
Tariq Ali ◽  
Nisar Ahmad ◽  
Zafran Khan ◽  
...  
Keyword(s):  
Seed Germination ◽  
Cold Stress ◽  
Brassica Rapa

scholarly journals Tolerance against ageing in different varieties of Brassica rapa var toria, B. rapa var yellow sarson and B. juncea( L.)

2018 ◽  
Vol 10 (1) ◽  
pp. 171-177
Author(s):  
Bhim Jyoti ◽  
Rambhajan Rambhajan ◽  
Ushapant Ushapant
Keyword(s):  
Developing Countries ◽  
Seed Germination ◽  
Laboratory Condition ◽  
Analysis Of Variance ◽  
Brassica Rapa ◽  
Seed Viability ◽  
Seed Deterioration ◽  
Brassica Spp ◽  
Aged Seed ◽  
Ageing Period

Seed deterioration is a serious problem in developing countries where seeds are stored in places usually without a proper control of humidity and temperature. In this investigation, an effort was made to identify crops as well as varieties of brassica spp. tolerance to ageing and predicting loss of seed viability at regular interval through standard germination under laboratory condition. Analysis of variance showed that mean squares due to crops, ageing periods and crop× ageing period were significant for % seed germination. Result revealed that standard germination declined from 95.63 to 37.54% in toria, 92 to 28.96 in yellow sarson, and 93.79 to 30.92% in mustard. Similar changes in germination % were observed at 9 and 12 months of observation. After 6 months, germination % declined from 95.13 to 35.50% in toria, 91.87 to 28% in yellow sarson and 93.33 to 29% in mustard. Analysis of variance showed significant differences for %seed germination due to varieties, ageing period and interaction of varieties × ageing periods. At varietal level AP2, AP3 and AP4 aged seed, Bhawani (82.66%, 68.16% and 28.17%) showed higher seed germination % respectively than other three varieties of toria. In case of yellowsarson and mus-tard least deterioration for standard germination were observed in B-9 and Kranti respectively.This study indicates that toria seeds have better storability than yeloowsarson and mustard. At varietal level good storage potential was found in Bhawani, B-9 and Krantivariety of toria, yellow sarson and mustard respectively than other three varieties of studied crops.

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