The water soluble Zn(II) and Mg(II) phthalocyanines: Synthesis, photochemical, DNA photodamage and PDT effects against A549 cells

Humans are typically exposed to environmental contaminants’ mixtures that result in different toxicity than exposure to the individual counterparts. Yet, the toxicology of chemical mixtures has been overlooked. This work aims at assessing and comparing viability and cell cycle of A549 cells after exposure to single and binary mixtures of: titanium dioxide nanoparticles (TiO2NP) 0.75–75 mg/L; cerium oxide nanoparticles (CeO2NP) 0.75–10 μg/L; arsenic (As) 0.75–2.5 mg/L; and mercury (Hg) 5–100 mg/L. Viability was assessed through water-soluble tetrazolium (WST-1) and thiazolyl blue tetrazolium bromide (MTT) (24 h exposure) and clonogenic (seven-day exposure) assays. Cell cycle alterations were explored by flow cytometry. Viability was affected in a dose- and time-dependent manner. Prolonged exposure caused inhibition of cell proliferation even at low concentrations. Cell-cycle progression was affected by TiO2NP 75 mg/L, and As 0.75 and 2.5 μg/L, increasing the cell proportion at G0/G1 phase. Combined exposure of TiO2NP or CeO2NP mitigated As adverse effects, increasing the cell surviving factor, but cell cycle alterations were still observed. Only CeO2NP co-exposure reduced Hg toxicity, translated in a decrease of cells in Sub-G1. Toxicity was diminished for both NPs co-exposure compared to its toxicity alone, but a marked toxicity for the highest concentrations was observed for longer exposures. These findings prove that joint toxicity of contaminants must not be disregarded.

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Size-Dependent Photodynamic Activity of Gold Nanoparticles Conjugate of Water Soluble Purpurin-18-N-Methyl-D-Glucamine

BioMed Research International ◽

10.1155/2013/720579 ◽

2013 ◽

Vol 2013 ◽

pp. 1-10 ◽

Cited By ~ 16

Author(s):

Byambajav Lkhagvadulam ◽

Jung Hwa Kim ◽

Il Yoon ◽

Young Key Shim

Keyword(s):

Gold Nanoparticles ◽

A549 Cells ◽

Water Soluble ◽

Molar Ratio ◽

Anticancer Efficacy ◽

Molar Ratios ◽

Size Dependent ◽

Photodynamic Activity ◽

Wavelength Absorption

Gold nanoparticles (GNPs) conjugates of water soluble ionic photosensitizer (PS), purpurin-18-N-methyl-D-glucamine (Pu-18-NMGA), were synthesized using various molar ratios between HAuCl4and Pu-18-NMGA without adding any particular reducing agents and surfactants. The PS-GNPs conjugates showed long wavelength absorption of range 702–762 nm, and their different shapes and diameters depend on the molar ratios used in the synthesis.In vitroanticancer efficacy of the PS-GNPs conjugates was investigated by MTT assay against A549 cells, resulting in higher photodynamic activity than that of the free Pu-18-NMGA. Among the PS-GNPs conjugates, the GNPs conjugate from the molar ratio of 1 : 2 (Au(III): Pu-18-NMGA) exhibits the highest photodynamic activity corresponding to bigger size (~60 nm) of the GNPs conjugate which could efficiently transport the PS into the cells than that of smaller size of the GNPs conjugate.

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Synthesis and biological studies of amphiphilic carbazole pyridinium conjugates

Journal of Porphyrins and Phthalocyanines ◽

10.1142/s1088424619501384 ◽

2020 ◽

Vol 24 (01n03) ◽

pp. 440-447

Author(s):

Vijayalakshmi Pandey ◽

Hilal Rather ◽

Sarla Yadav ◽

Rajesh Vasita ◽

Iti Gupta

Keyword(s):

A549 Cells ◽

Emission Spectra ◽

Water Soluble ◽

Absorption And Emission ◽

Carbazole Derivatives ◽

Alkyl Chains ◽

Biological Studies ◽

Stokes Shifts ◽

Pyridinium Group ◽

C Nmr

Amphiphilic carbazole pyridinium conjugates are synthesized and characterized by IR, 1H and [Formula: see text]C NMR and ESI-MS spectrometry. The pyridinium group is attached on the 3-position of the carbazole ring and long alkyl chains are linked to the central [Formula: see text] atom. The introduction of a pyridinium group afforded water-soluble carbazole derivatives with significant bathochromic shifts in their absorption and emission spectra. As compared to the parent [Formula: see text]-butylcarbazole compound, carbazole pyridinium conjugates exhibited 50 nm red-shifted absorption maxima. Similarly, the carbazole pyridinium conjugates displayed 143–147 nm red-shifted emission maxima in solution. In addition, large Stokes shifts (5747–7558 cm[Formula: see text] were observed for the conjugates in solution. The cell penetrable amphiphilic carbazole pyridinium conjugates exhibited cytoplasmic distribution in A549 cells.

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Toxicity of TiO2, ZnO, and SiO2 Nanoparticles in Human Lung Cells: Safe-by-Design Development of Construction Materials

Nanomaterials ◽

10.3390/nano9070968 ◽

2019 ◽

Vol 9 (7) ◽

pp. 968 ◽

Cited By ~ 6

Author(s):

Monika Remzova ◽

Radek Zouzelka ◽

Tana Brzicova ◽

Kristyna Vrbova ◽

Dominik Pinkas ◽

...

Keyword(s):

Early Stage ◽

Construction Materials ◽

A549 Cells ◽

Sio2 Nanoparticles ◽

Water Soluble ◽

Design Development ◽

Toxicological Effects ◽

Human Lung Cells ◽

Low Toxicity ◽

Pristine Tio2

Rapid progress in the development of highly efficient nanoparticle-based construction technologies has not always been accompanied by a corresponding understanding of their effects on human health and ecosystems. In this study, we compare the toxicological effects of pristine TiO2, ZnO, SiO2, and coated SiO2 nanoparticles, and evaluate their suitability as additives to consolidants of weathered construction materials. First, water soluble tetrazolium 1 (WST-1) and lactate dehydrogenase (LDH) assays were used to determine the viability of human alveolar A549 cells at various nanoparticle concentrations (0–250 μg mL−1). While the pristine TiO2 and coated SiO2 nanoparticles did not exhibit any cytotoxic effects up to the highest tested concentration, the pristine SiO2 and ZnO nanoparticles significantly reduced cell viability. Second, as all developed nanoparticle-modified consolidants increased the mechanical strength of weathered sandstone, the decisive criterion for the selection of the most suitable nanoparticle additive was as low toxicity as possible. We believe that this approach would be of high importance in the industry, to identify materials representing top functional properties and low toxicity, at an early stage of the product development.

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Influence of the PM2.5 Water-Soluble Compound on the Biophysical Properties of A549 Cells

Langmuir ◽

10.1021/acs.langmuir.1c00522 ◽

2021 ◽

Vol 37 (13) ◽

pp. 4042-4048

Author(s):

Mingjie Tang ◽

Yan Wang ◽

Dongyun Tang ◽

Peng Xiu ◽

Zhongbo Yang ◽

...

Keyword(s):

A549 Cells ◽

Water Soluble ◽

Biophysical Properties ◽

Water Soluble Compound ◽

Soluble Compound

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Effects of water-soluble components of atmospheric particulates from rare earth mining areas in China on lung cancer cell cycle

Particle and Fibre Toxicology ◽

10.1186/s12989-021-00416-z ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Yuan Xia ◽

Xulong Zhang ◽

Dejun Sun ◽

Yumin Gao ◽

Xiaoe Zhang ◽

...

Keyword(s):

Lung Cancer ◽

Cell Cycle ◽

Rare Earth ◽

A549 Cell ◽

A549 Cells ◽

Cyclin B1 ◽

Water Soluble ◽

Differentially Expressed ◽

M Phase ◽

Cell Arrest

Abstract Background This study aims to investigate the effects of water soluble particulate matter (WSPM) on the viability and protein expression profile of human lung adenocarcinoma cell A549 in the Bayou Obo rare earth mining area, and explore the influence of WSPM on the A549 cell cycle. Results It was found that WSPM can inhibit the viability of A549 cells and induce cell arrest in the G2/M phase. Compared with controls, exposure to WSPM10 and WSPM2.5 induced 134 and 116 proteins to be differentially expressed in A549 cells, respectively. In addition, 33 and 31 differentially expressed proteins were further confirmed, and was consistent with the proteomic analysis. The most prominent enrichment in ribosome-associated proteins were presented. When RPL6, RPL13, or RPL18A gene expression was inhibited, A549 cells were arrested in the G1 phase, affecting the expression of Cyclin D1, p21, RB1, Cyclin A2, Cyclin B1, CDC25A, CDK2, CHEK2 and E2F1. Furthermore, the La3+, Ce3+, Nd3+ and F- in WSPM also inhibited the viability of A549 cells. After 24 h of exposure to 2 mM of NaF, A549 cells were also arrested in the G2/M phase, while the other three compounds did not have this effect. These four compounds affected the cell cycle regulatory factors in A549 cells, mainly focusing on effecting the expression of CDK2, CDK4, RB1, ATM, TP53 and MDM2 genes. These results are consistent with the those from WSPM exposure. Conclusions These results revealed that WSPM from rare earth mines decreased the viability of A549 cells, and induced cell cycle G2/M phase arrest, and even apoptosis, which may be independent of the NF-κB/MYD88 pathway, and be perceived by the TLR4 receptor. The dysfunction of the cell cycle is correlated to the down-expression of ribosomal proteins (RPs). However, it is not the direct reason for the A549 cell arrest in the G2/M phase. La3+, Ce3+, and F- are probably the main toxic substances in WSPM, and may be regulate the A549 cell cycle by affecting the expression of genes, such as MDM2, RB1, ATM, TP53, E2F1, CDK2 and CDK4. These results indicate the importance for further research into the relationship between APM and lung cancer.

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A Facile One-Pot Synthesis of Water-Soluble, Patchy Fe3O4-Au Nanoparticles for Application in Radiation Therapy

Applied Sciences ◽

10.3390/app9010015 ◽

2018 ◽

Vol 9 (1) ◽

pp. 15 ◽

Cited By ~ 5

Author(s):

Stefanie Klein ◽

Jakob Hübner ◽

Christina Menter ◽

Luitpold Distel ◽

Winfried Neuhuber ◽

...

Keyword(s):

Au Nanoparticles ◽

A549 Cells ◽

Water Soluble ◽

X Rays ◽

Gold Nanocluster ◽

One Pot ◽

X Ray ◽

One Pot Synthesis ◽

Fe3o4 Nps ◽

Mcf 7

A facile one-pot synthesis route for the preparation of water-soluble, biocompatible patchy Fe3O4-Au nanoparticles (Fe3O4-Au pNPs) was developed. Biocompatibility was attained through surface functionalization with 1-methyl-3-(dodecylphosphonic acid) imidazolium bromide. The morphology, composition, crystal structure and magnetic properties of the Fe3O4-Au pNPs were investigated by conducting experiments with transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and superconducting quantum interference device, respectively. Internalization of the Fe3O4-Au pNPs by MCF-7 cells occurred via endocytosis. The performance of the Fe3O4-Au pNPs as X-ray radiosensitizer in tumor cells was compared with that of gold nanocluster and Fe3O4 NPs. For this reason, MCF-7, A549 and MCF-10A cells were loaded with the respective kind of nanoparticles and treated with X-rays at doses of 1, 2 or 3 Gy. The nanoparticle-induced changes of the concentration of the reactive oxygen species (ROS) were detected using specific assays, and the cell survival under X-ray exposure was assessed employing the clonogenic assay. In comparison with the gold nanocluster and Fe3O4 NPs, the Fe3O4-Au pNPs exhibited the highest catalytic capacity for ROS generation in MCF-7 and A549 cells, whereas in the X-ray-induced ROS formation in healthy MCF-10A cells was hardly enhanced by the Fe3O4 NPs and Fe3O4-Au pNPs. Moreover, the excellent performance of Fe3O4-Au pNPs as X-ray radiosensitizers was verified by the quickly decaying radiation dose survival curve of the nanoparticle-loaded MCF-7 and A549 cells and corroborated by the small values of the associated dose-modifying factors.

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Novel Water-Soluble Far-Red Nitroreductase-Responsive Bodipy-Based Fluorescent Probe for the Detection of Hypoxic Status in A549 Non-Small Cell Lung Cancer Cells

10.26434/chemrxiv.12600488.v1 ◽

2020 ◽

Author(s):

Sergey A. GORBATOV ◽

Denis Y. UVAROV ◽

Alexander M. SCHERBAKOV ◽

Igor V. ZAVARZIN ◽

Yulia A. VOLKOVA ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Medical Diagnosis ◽

A549 Cells ◽

Small Cell ◽

Water Soluble ◽

Small Cell Lung ◽

Over Expression ◽

Living Organisms

Over expression of nitroreductase (NTR) enzymes is closely related to the hypoxic status in living organisms for which molecular oxygen is vital. The development of effective methods for real-time monitoring of NTR activity is of great significance for medical diagnosis and cancer research. Here, we present a novel water-soluble Bodipy-based chemodosimeter (NBB) effective in imaging the hypoxic status of human non-small-cell lung cancer A549 cells. We assumed that NTR-mediated activation of the probe NBB is based on the reductive release of meso-phenol Bodipy dye to finally produce unusual fluorescence "ON-OFF" response.

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Novel Water-Soluble Far-Red Nitroreductase-Responsive Bodipy-Based Fluorescent Probe for the Detection of Hypoxic Status in A549 Non-Small Cell Lung Cancer Cells

10.26434/chemrxiv.12600488 ◽

2020 ◽

Author(s):

Sergey A. GORBATOV ◽

Denis Y. UVAROV ◽

Alexander M. SCHERBAKOV ◽

Igor V. ZAVARZIN ◽

Yulia A. VOLKOVA ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Cell Lung Cancer ◽

Medical Diagnosis ◽

A549 Cells ◽

Small Cell ◽

Water Soluble ◽

Small Cell Lung ◽

Over Expression ◽

Living Organisms

Over expression of nitroreductase (NTR) enzymes is closely related to the hypoxic status in living organisms for which molecular oxygen is vital. The development of effective methods for real-time monitoring of NTR activity is of great significance for medical diagnosis and cancer research. Here, we present a novel water-soluble Bodipy-based chemodosimeter (NBB) effective in imaging the hypoxic status of human non-small-cell lung cancer A549 cells. We assumed that NTR-mediated activation of the probe NBB is based on the reductive release of meso-phenol Bodipy dye to finally produce unusual fluorescence "ON-OFF" response.

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