A highly sensitive naphthalimide based fluorescent “turn-on” sensor for H2S and its bio-imaging applications

2022 ◽  
pp. 113802
Author(s):  
Dhanapal Jothi ◽  
Sathiyanarayanan Kulathu Iyer
Keyword(s):  
Turn On ◽  
Highly Sensitive ◽  
Bio Imaging

scholarly journals Development of a Novel Benzimidazole-Based Probe and Portable Fluorimeter for the Detection of Cysteine in Human Urine

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 420
Author(s):  
Gyu Seong Yeom ◽  
In-ho Song ◽  
Shrikant Dashrath Warkad ◽  
Pramod B. Shinde ◽  
Taewoon Kim ◽  
...  
Keyword(s):  
Amino Acids ◽  
Dna Damage ◽  
Human Urine ◽  
Live Cells ◽  
Turn On ◽  
Highly Sensitive ◽  
Bio Imaging ◽  
Fluorescence Turn On ◽  
Fluorescence Spectrometer ◽  
Excellent Selectivity

The measurement of cysteine in human urine and live cells is crucial for evaluating biological metabolism, monitoring and maintaining the immune system, preventing tissue/DNA damage caused by free radicals, preventing autoimmune diseases, and diagnosing disorders such as cystinuria and cancer. A method that uses a fluorescence turn-on probe and a portable fluorescence spectrometer device are crucial for highly sensitive, simple, rapid, and inexpensive cysteine detection. Herein, we present the synthesis and application of a benzimidazole-based fluorescent probe (ABIA) along with the design and development of a portable fluorescence spectrometer device (CysDDev) for detecting cysteine in simulated human urine. ABIA showed excellent selectivity and sensitivity in detecting cysteine over homocysteine, glutathione, and other amino acids with the response time of 1 min and demonstrated a detection limit of 16.3 nM using the developed CysDDev. Further, ABIA also demonstrated its utility in detecting intracellular cysteine, making it an excellent probe for bio-imaging assay.

A phenanthrene[9,10-d]imidazole-phenol-based fluorescent probe combined ESIPT and AIE for “turn-on” detection of Cu2+ with a green-emitting and improved stokes shift and its application

2021 ◽  
Author(s):  
Jiahui Du ◽  
Bing Zhao ◽  
Wei Kan ◽  
Haochun Yin ◽  
Tianshu Song ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
Fluorescent Sensors ◽  
Turn On ◽  
Highly Sensitive

Development of highly sensitive and selective fluorescent sensors toward Cu2+ has gained considerable attention in view of its application of environmental and biological fields. However, the strategy of sensing by...

A highly-sensitive “turn on” probe based on coumarin β-diketone for hydrazine detection in PBS and living cells

2021 ◽  
Vol 252 ◽  
pp. 119510
Author(s):  
Rui Chen ◽  
Guang-Jin Shi ◽  
Jia-Jia Wang ◽  
Hai-Feng Qin ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Turn On ◽  
Highly Sensitive

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

A highly sensitive turn-on ratiometric luminescent probe based on postsynthetic modification of Tb3+@Cu-MOF for H2S detection

2017 ◽  
Vol 5 (38) ◽  
pp. 9943-9951 ◽  
Author(s):  
Xubin Zheng ◽  
Ruiqing Fan ◽  
Yang Song ◽  
Ani Wang ◽  
Kai Xing ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Luminescent Probe ◽  
Turn On ◽  
Postsynthetic Modification ◽  
Highly Sensitive ◽  
Sensitivity And Selectivity ◽  
H2s Detection

A novel probe, designed and prepared through PSM of Cu-MOFs and Tb3+ions, shows high sensitivity and selectivity for H2S.

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