Comparative analysis of NK cell subsets in menstrual and peripheral blood of patients with unexplained recurrent spontaneous abortion and fertile subjects

2014 ◽  
Vol 103 ◽  
pp. 9-17 ◽  
Author(s):  
Samira Hosseini ◽  
Amir-Hassan Zarnani ◽  
Hossein Asgarian-Omran ◽  
Zohreh Vahedian-Dargahi ◽  
Mohammad Reza Eshraghian ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Spontaneous Abortion ◽  
Peripheral Blood ◽  
Nk Cell ◽  
Recurrent Spontaneous Abortion ◽  
Unexplained Recurrent Spontaneous Abortion

scholarly journals Distinct changes of in BTLA, ICOS, PD-1, and TIGIT expression on peripheral blood and decidual CD8+ T cells in women with unexplained recurrent spontaneous abortion†

2020 ◽  
Vol 103 (5) ◽  
pp. 1012-1017
Author(s):  
Qianqian Liang ◽  
Lingxia Tong ◽  
Liping Xiang ◽  
Sujuan Shen ◽  
Chenhuan Pan ◽  
...  
Keyword(s):  
T Cells ◽  
Spontaneous Abortion ◽  
Cd8 T Cells ◽  
Immune Cells ◽  
Peripheral Blood ◽  
Immune Escape ◽  
Recurrent Spontaneous Abortion ◽  
Decidual Tissue ◽  
Unexplained Recurrent Spontaneous Abortion ◽  
The Difference

Abstract The two-way communication between the mother and the fetus is accomplished by immune cells. CD8+ T cells of normal pregnant (NP) women express progesterone receptor (PR). Binding of PR to progesterone (P) and the production of progesterone-induced blocking factor (PIBF) can aid immune escape, which is an important factor in the maternal immune response. We detected the proportion of CD8+ T cells and the expression of the surface costimulatory molecules BTLA, TIGIT, ICOS, and PD-1 in peripheral blood and decidual tissues of women with unexplained recurrent spontaneous abortion (URSA) and in NP women. All patients were at 8 -10 weeks of gestation. The results showed that there was no change in the proportions of CD8+ T cells in peripheral blood and decidual tissues of URSA patients compared to those of NP women. In peripheral blood, compared with the NP group, the URSA group showed decreased expression of BTLA + CD8+ T cells and the difference was statistically significant, but there was no difference between the groups in terms of TIGIT + CD8+, PD-1 + CD8+, and ICOS + CD8+ T cells. There was no change in the levels of TIGIT + CD8+, PD-1 + CD8+, ICOS + CD8+, and BTLA + CD8+ T cells in decidual tissue. These data confirm that the number of CD8+ T cells in peripheral blood and decidual tissue is not the main factor leading to the pathogenesis of URSA, and other immune cells may play an important role in URSA, but this hypothesis needs further exploration and research.

scholarly journals Evaluation of the Effects of 1,25VitD3 on Inflammatory Responses and IL-25 Expression

2021 ◽  
Vol 12 ◽  
Author(s):  
Nana Li ◽  
Nafiseh Saghafi ◽  
Zahra Ghaneifar ◽  
Seyed Abdorahim Rezaee ◽  
Houshang Rafatpanah ◽  
...  
Keyword(s):  
Spontaneous Abortion ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Mononuclear Cells ◽  
Inflammatory Responses ◽  
Recurrent Spontaneous Abortion ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Unexplained Recurrent Spontaneous Abortion ◽  
Blood Mononuclear Cells

VitD3 may contribute to a successful pregnancy through modulation of immune responses, so VitD3 deficiency may have a role in the immunopathogenesis of unexplained recurrent spontaneous abortion (URSA). However, the mechanisms of immunomodulatory actions of VitD3 in decreasing the risk of recurrent spontaneous abortion have not been understood well.Objective: The purpose of this research was to investigate the influence of 1,25VitD3 on IL-25 and related cytokines of Th17 cells including IL-17A, IL-6, and IL-23 in peripheral blood mononuclear cells of healthy women as a control group and women with unexplained recurrent spontaneous abortion.Method: Isolation of peripheral blood mononuclear cells (PBMCs) was performed from peripheral blood of the subjects of the studied groups (20 women with URSA as a case group, and 20 control women). The effects of 1,25VitD3 (50 nM, for 24 h) on the studied parameters were evaluated and were compared to the positive and negative controls in vitro. Flow cytometry analysis was used to determine the percentages of regulatory T cells and Th17 cells. For gene expression measurement and cytokines assay, real-time PCR and ELISA were carried out.Results: The proportion of Th17 cells in women with URSA was considerably higher than in the control group. IL-25 mRNA and protein levels in cultured PBMCs from women with URSA were lower than the controls. 1,25VitD3 increased IL-25 expressions at both the protein and mRNA levels in PBMCs from women with URSA relative to the control group. Additionally, 1,25VitD3 treatment not only significantly decreased the percentage of Th17 cells frequency but also reduced expressions of IL-6, IL-17A, and IL-23 in PBMCs from women with URSA.Conclusion: 1,25VitD3 may diminish inflammatory responses cells via downregulation of IL-25 expression. It could be an interesting subject for future researches in the field of the immunopathology of URSA to identify molecular pathways in URSA treatment.

scholarly journals Significance to investigate the TH1 /TH2 cytokines and their proportions in peripheral blood of women with unexplained recurrent spontaneous abortion and normal women based on pregnancy status

2020 ◽  
Author(s):  
Yizhi Peng ◽  
Min Wang
Keyword(s):  
Spontaneous Abortion ◽  
Peripheral Blood ◽  
Recurrent Spontaneous Abortion ◽  
Th2 Cytokines ◽  
Diagnostic Efficiency ◽  
Th2 Immune Response ◽  
Unexplained Recurrent Spontaneous Abortion ◽  
Ifn Γ ◽  
Adverse Pregnancy ◽  
Normal Women

Abstract Background: To investigate the significance of th1/th2 cytokines in the pathogenesis of unexplained recurrent spontaneous abortion(URSA) patients. Methods: Flow cytometry was used to determine the level of th1/th2 cytokines in peripheral blood of URSA patients, comparison between two groups was analysed by Mann-Whitney U test, and Kruskal-Wallis H test was used to test three or more groups. Results : T he content of il-6 in peripheral blood of adverse pregnancy group (ad-pregnancy group) was significantly higher than that of URSA group and pregnancy group. The ratio of il-2 / il-4 and IFN-γ/il-4 in peripheral blood of URSA group was significantly higher than that of pregnancy group, the ratio of IFN-γ/il-10 and IFN-γ/il-4 in peripheral blood of ad-pregnancy group was significantly higher than that of pregnancy group. The AUC of IFN-γ/il-4 was 0.821, with high diagnostic efficiency, sensitivity as high as 84.09% and specificity as high as 69.05%, which were higher than other indicators. Conclusion : The Variable of IFN-γ/il-4 can be used for the initial diagnosis of Ursa to reduce the rate of missed diagnosis, the cytokine ratio in the Th1/Th2 immune response was more important than the expression of single cytokine.

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