Studies of TGF-β1-3 in Serosal Fluid During Abdominal Surgery and Their Effect on In Vitro Human Mesothelial Cell Proliferation

2009 ◽  
Vol 154 (2) ◽  
pp. 312-316 ◽  
Author(s):  
Peter Falk ◽  
Maria Bergström ◽  
Ingrid Palmgren ◽  
Lena Holmdahl ◽  
Michael E. Breimer ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Abdominal Surgery ◽  
Mesothelial Cell ◽  
Human Mesothelial Cell ◽  
Tgf Β1

scholarly journals Effect of osmotic solutes on human mesothelial cell proliferation in vitro.

1999 ◽  
Vol 32 (7) ◽  
pp. 1065-1070
Author(s):  
Ping Ma ◽  
Toru Hyodo ◽  
Shinji Yokota ◽  
Kazuo Kumano ◽  
Tadasu Sakai
Keyword(s):  
Cell Proliferation ◽  
Mesothelial Cell ◽  
Osmotic Solutes ◽  
Proliferation In Vitro ◽  
Human Mesothelial Cell

Biocompatibility of a Bicarbonate/Lactate-Buffered PD Fluid Tested with a Double-Chamber Cell Culture System

2005 ◽  
Vol 25 (4) ◽  
pp. 387-393 ◽  
Author(s):  
Andreas Fusshoeller ◽  
Jessica Baehr ◽  
Bernd Grabensee ◽  
Joerg Plum
Keyword(s):  
Cell Culture ◽  
Cell Viability ◽  
Culture System ◽  
Mesothelial Cell ◽  
In Vitro Studies ◽  
Cell Culture System ◽  
And Function ◽  
Tgf Β1 ◽  
Double Chamber

Objective In peritoneal dialysis (PD), neutrally buffered PD fluids with lower concentrations of glucose degradation products (GDP) have tested superior to conventional fluids in terms of biocompatibility. However, conventional in vitro studies provoke debate because, due to the lack of subsequent equilibration with the blood, they do not resemble the true intraperitoneal situation of PD. Methods We established a double-chamber cell culture system with peritoneal mesothelial cells seeded on top of a permeable membrane, with a physiological buffer below. Thus adequately reflecting the in vivo equilibration pattern, we compared a conventional fluid with a neutral bicarbonate/lactate-buffered PD solution. Using an exchange pattern adapted from an 8-hour continuous ambulatory PD regimen, cell viability was assessed with an MTT assay, and cell function via constitutive and stimulated interleukin (IL)-6 release. As an indicator of potential induction of fibrosis and as a parameter of mesothelial cell integrity, respectively, transforming growth factor-beta 1 (TGF-β1) generation and cancer antigen 125 (CA125) release were measured. Results The conventional solution significantly compromised mesothelial cell viability and function in terms of mitochondrial activity ( p < 0.05) and stimulated IL-6 release ( p < 0.05). The bicarbonate/lactate fluid had no effect on cell viability or IL-6 release and turned out to be equivalent to the properties of the growth medium. Whereas lactate-incubated cells did not respond to IL-1β stimulation, bicarbonate/lactate-treated cells adequately increased IL-6 release after stimulation ( p < 0.0005). Release of TGF-β1 and CA125 did not differ between the different fluids and the control. Conclusions Due to the sustained equilibration process, the double-chamber cell culture model allows a more realistic insight into mesothelial cell viability and function in terms of PD. As in classic in vitro studies, an adverse effect of conventional PD solutions on mesothelial cells was overt in the present cell culture system. The neutral bicarbonate/lactate-buffered fluid with low GDP content, however, did not interfere with mesothelial cell vitality or function, indicating superior biocompatibility.

scholarly journals MiRNA-21 Regulates Bronchial Epithelial Cell Proliferation by Activating Tgfβ1/Smad Signaling Pathway and Its Correlation with Asthma Severity in Children

2021 ◽  
Author(s):  
Yang Kang ◽  
Minghui Bai ◽  
Liling Deng ◽  
Linbo Fan ◽  
Xing Wang
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathway ◽  
Bronchial Epithelial Cell ◽  
Control Group ◽  
Healthy Children ◽  
Bronchial Epithelial ◽  
Smad Signaling ◽  
Smad Signaling Pathway ◽  
Tgf Β1

Background: This research was designed to probe into the role of miRNA-21 in the pathogenesis of childhood asthma and its correlation with the severity. Methods: Fifty-four children with bronchial asthma admitted to the Third Affiliated Hospital of Qiqihar Medical University from Jun 2018 to Dec 2019 were included. Forty nine healthy children underwent physical examination at this time period were also enrolled. The miR-21 expression in peripheral blood serum was analyzed by qRT-PCR. The relationship between the expression and severity of asthma in children was explored by Spearman correlation analysis and ROC curve. Bronchial epithelial cell lines were cultured in vitro and divided into blank control group, negative control group and miR-21 inhibition and activation group. The changes of cell proliferation after treatment were detected by CCK-8 test in different groups. The expression of TGF-β1/Smad signaling pathway protein in cells was assessed by Western blot (WB). Results: Compared with that of healthy children, the miR-21 expression in peripheral blood serum of asthmatic children was higher (P<0.001). MiR-21 expression was positively correlated with the severity of illness (r=0.853, P<0.001). The results of cell experiments in vitro signified that miR-21 can promote the proliferation of bronchial epithelial cells, and may be involved in regulating the expression of TGF-β1/ Smad3 signaling pathway, thus affecting cell proliferation. Conclusion: miRNA-21 regulates the proliferation of bronchial epithelial cells by activating TGFβ1/Smad signaling pathway. And it is positively correlated with the severity of asthma in children.

Improved In Vitro Biocompatibility of Bicarbonate-Buffered Peritoneal Dialysis Fluid

2006 ◽  
Vol 26 (6) ◽  
pp. 664-670 ◽  
Author(s):  
Nicolas Grossin ◽  
Marie-Paule Wautier ◽  
Jean-Luc Wautier ◽  
Pierre Gane ◽  
Redouane Taamma ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Peritoneal Dialysis ◽  
Growth Factor ◽  
Cell Biology ◽  
Transforming Growth Factor ◽  
Mesothelial Cell ◽  
Long Term Treatment ◽  
Peritoneal Dialysis Fluid ◽  
New Generation

Background Conventional peritoneal dialysis fluids (PDFs) have been shown to damage the mesothelial layer and are associated with the development of peritoneal fibrosis and neoangiogenesis. New-generation PDFs have therefore been developed with physiological pH and reduced levels of glucose degradation products (GDPs), precursors of advanced glycation end products (AGEs). In this work, we evaluated and compared the improved biocompatibility of two new-generation PDFs (Balance and bicaVera) using mesothelial cell biology; we also compared them to a standard PDF (stay·safe) (all PDFs by Fresenius Medical Care, Fresnes, France). Methods stay·safe, Balance, and bicaVera were tested for their effect on human peritoneal mesothelial cell (HPMC) viability by measuring cell proliferation and apoptosis, and oncosis induction. The formation of AGEs was evaluated by immunoassay. Transforming growth factor beta-1 and vascular endothelial growth factor (VEGF) were immunoassayed in HPMC supernatants exposed to the above PDFs. Results At 15 g/L glucose concentration, HPMC exposure to bicaVera resulted in higher cell proliferation compared to Balance ( p < 0.001) and stay·safe ( p < 0.001). Compared to the lactate-buffered PDFs (Balance and stay·safe), oncosis was significantly lower in cells exposed to bicaVera ( p < 0.05). bicaVera, containing lower amounts of GDPs, generated less AGE formation ( p < 0.05) and VEGF production ( p < 0.05) than either Balance or stay·safe. Conclusions New-generation PDFs with physiological pH and lower GDP levels, especially if bicarbonate-buffered (bicaVera), have fewer in vitro toxic effects on mesothelial cells and may contribute to peritoneal preservation, thus improving long-term treatment of PD patients.

scholarly journals Reactivity of integrin-linked kinase in human mesothelial cell proliferation

2008 ◽  
Vol 7 (1) ◽  
pp. 107-110 ◽  
Author(s):  
S. B. Watzka ◽  
U. Setinek ◽  
M. Huber ◽  
H. Cantonati ◽  
F. Lax ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Mesothelial Cell ◽  
Integrin Linked Kinase ◽  
Human Mesothelial Cell

Biological activities of commercial bovine lactoferrin sources

2020 ◽  
Author(s):  
Bo Lönnerdal ◽  
Xiaogu Du ◽  
Rulan Jiang
Keyword(s):  
Cell Proliferation ◽  
Biological Activities ◽  
Intestinal Cell ◽  
Bovine Lactoferrin ◽  
Cell Proliferation And Differentiation ◽  
Sds Page ◽  
Proliferation And Differentiation ◽  
Intestinal Proliferation ◽  
Tgf Β1

Lactoferrin (Lf) samples from several manufacturers were evaluated in vitro. The purity and protein form of each Lf were examined by SDS-PAGE, Western blot, and proteomics analysis. Assays were conducted to evaluate uptake of Lfs and iron from Lfs by enterocytes as well as Lf bioactivities, including effects on intestinal cell proliferation and differentiation, IL-18 secretion, TGF-β1 transcription, and growth of enteropathogenic Escherichia coli (EPEC). Composition of the Lfs varied; some only contain a major Lf band (~80 kDa), and some also contain minor forms. All Lfs and iron from the Lfs were absorbed by Caco-2 cells with varying efficiencies. The bioactivities of the Lfs varied considerably, but there was no consistent trend. All Lfs promoted intestinal cell proliferation, secretion of IL-18, and transcription of TGF-β1. Some Lfs exhibited pro-differentiation effects on Caco-2 cells. Effects of pasteurization (62.5°C for 30 min, 72°C for 15 sec, or 121°C for 5 min) on integrity, uptake and bioactivities were examined using Dicofarm, Tatua, and native bovine Lfs. Results show that pasteurization did not affect protein integrity, but variously affected uptake of Lf, and its effects on intestinal proliferation, differentiation, and EPEC growth. To choose a Lf source for a clinical trial, assessment of bioactivities is recommended.

TMEM88 inhibits TGF-β1-induced-extracellular matrix (ECM) accumulation and epithelial-mesenchymal transition (EMT) program in human pleural mesothelial cells through modulating TGF-β1/Smad pathway

2020 ◽  
Author(s):  
Zhongmin Sun ◽  
Ning Qian ◽  
Hong Li ◽  
Tinghua Hu ◽  
Ling Tang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Mesenchymal Transition ◽  
Mesothelial Cell ◽  
Pathological Process ◽  
Mesenchymal Transition ◽  
Expression Levels ◽  
Pleural Fibrosis ◽  
Tgf Β1

Abstract Pleural fibrosis is an irreversible pathological process occurred in the development of several lung diseases. TMEM88 is a member of transmembrane (TMEM) family and has been found to be involved in the regulation of fibrogenesis. However, the role of TMEM88 in pleural fibrosis remains unknown. In this study, we aimed to explore the role of TMEM88 in pleural fibrosis in vitro using TGF-β1-induced human pleural mesothelial cell line MeT-5A cells. Our results showed that the expression levels of TMEM88 were downregulated in pleural fibrosis tissues and TGF-β1-treated Met-5A cells. Overexpression of TMEM88 inhibited the proliferation of Met-5A cells under TGF-β1 stimulation. In addition, TMEM88 overexpression prevented TGF-β1-induced extracellular matrix (ECM) accumulation and epithelial-mesenchymal transition (EMT) in Met-5A cells with decreased expression levels of Col I and fibronectin, increased levels of cytokeratin-8 and E-cadherin, as well as decreased levels of vimentin and α-SMA. Furthermore, overexpression of TMEM88 inhibited the expression of TGF-β receptor I (TβRI) and TβRII and suppressed the phosphorylation of Smad2 and Smad3 in Met-5A cells. In conclusion, these results indicated that TMEM88 exhibited an anti-fibrotic activity in pleural fibrosis via inhibiting the activation of TGF-β1/Smad signaling pathway.

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