Chrysin Activates the Aryl Hydrocarbon Receptor and Reduces Colon Cancer Cell Viability

Abstract: The release of lysosomal hydrolase into the cytoplasm is accompanied by several systems of apoptosis signal transduction, and the imbalance between cell viability and apoptosis induce tumorigenesis. Tea polysaccharide...

Download Full-text

Investigation of antioxidant activity and effect on cell viability of pyrrolidine compounds in MCF-7 (breast cancer) and DLD-1 (colon cancer) cell lines

International Journal of Biology and Chemistry ◽

10.26577/ijbch.2021.v14.i1.03 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Seda Mesci ◽

Melek Gül ◽

Tuba Yıldırım

Keyword(s):

Breast Cancer ◽

Colon Cancer ◽

Antioxidant Activity ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines ◽

Mcf 7

Download Full-text

An Investigation on the in vitro Effects of Elaidic Acid and Gamma-Linolenic Acid on the Survival of MDA-MB-231 and RKO Cell Lines

Proceedings International ◽

10.33263/proceedings21.012012 ◽

2020 ◽

Vol 2 (1) ◽

pp. 12

Keyword(s):

Breast Cancer ◽

Colon Cancer ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Statistical Difference ◽

Cancer Cell Line ◽

Colon Cancer Cell ◽

Gamma Linolenic Acid

Colorectal and breast cancer are a major cause of mortality worldwide. They can be caused due to an array of factors, of which diet plays an important role. Previous studies have suggested that Elaidic acid (EA), a trans fatty acid, supports the growth of colon cancer cell lines, like-RKO, LoVo, and HT29;. In contrast, Gamma-linolenic acid (GLA), a poly-unsaturated fatty acid, has tumoricidal effects. Therefore, the objective of this study was to investigate and delineate the in vitro dose-dependent effects of EA and GLA on the survival of MDA-MB-231(breast cancer cell line) and RKO cells (colon cancer cell line), through cell viability assay. The principal findings of this study were that EA induced a stimulatory effect. At the same time, GLA had an inhibitory effect on both cell lines. There was no statistical significance in the percentage viability of the MDA-MB-231 cells after treatment with EA. At the same time, there was a statistical difference in percentage viability after treatment with GLA. The highest test concentration of GLA that caused approximately 99% inhibition of MDA-MB-231 cells was 500µM, and its IC50 was mathematically calculated to be 239.687µM. For RKO cells, there was a statistical difference in percentage cell viability after treatment with EA. At the same time, there was no statistical difference in percentage cell viability after treatment with GLA. These results suggested that MDA-MB-231 cells were more susceptible to the effects of EA and GLA and also that EA should be consumed in moderation, while GLA appeared as a promising therapeutic option either by itself or in combination with other chemotherapeutic drugs.

Download Full-text

Cytotoxic, antimigratory, pro-and antioxidative activities of extracts from medicinal mushrooms on colon cancer cell lines

Archives of Biological Sciences ◽

10.2298/abs150427131s ◽

2016 ◽

Vol 68 (1) ◽

pp. 93-105 ◽

Cited By ~ 4

Author(s):

Dragana Seklic ◽

Milan Stankovic ◽

Milena Milutinovic ◽

Marina Topuzovic ◽

Andras Stajn ◽

...

Keyword(s):

Oxidative Stress ◽

Colon Cancer ◽

Cell Viability ◽

Cell Lines ◽

Cancer Cell ◽

Antioxidant Status ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines ◽

Hct 116

Methanol extracts of five commercially available mushroom species (Phellinus linteus (Berk. et Curt) Teng, Cordyceps sinensis (Berk.) Sacc., Lentinus edodes (Berk.) Pegler, Coprinus comatus (O. F. M?ll.) Pers. and Ganoderma lucidum (Curtis) P. Karst), traditionally used as anticancer agents, were evaluated in vitro for their total phenol and flavonoid contents, cytotoxic and antimigratory activities and antioxidant/prooxidant effects on colon cancer cell lines (HCT-116 and SW-480). Spectrophotometric methods were used for the determination of total phenol content, flavonoid concentrations and DPPH activity of the extracts. Cytotoxic activity was measured by the MTT assay. The antimigratory activity of extracts was determined using the Transwell assay and immunofluorescence staining of ?-catenin. The prooxidant/antioxidant status was followed by measuring the superoxide anion radical (O2?-), nitrite and reduced glutathione (GSH) concentrations. Our results show that the highest phenolic and flavonoid content was found in P. linteus, and its DPPH-scavenging capacity was significantly higher than in other samples. The P. linteus extract significantly decreased cell viability of both tested cancer cell lines. All other extracts selectively inhibited SW-480 cell viability, but did not show significant cytotoxic activity. The mushroom extracts caused changes in the prooxidant/antioxidant status of cells, inducing oxidative stress. All extracts tested on HCT-116 cells demonstrated significant antimigratory effects, which correlated with increased production of O2?- and a reduced level of ?-catenin protein expression, while only P. linteus showed the same effect on SW-480 cells. The results of the present research indicate that the mushroom extracts causes oxidative stress which has a pronounced impact on the migratory status of colon cancer cell lines.

Download Full-text

Pharmacological Inhibition of CDK8 in Triple-Negative Breast Cancer Cell Line MDA-MB-468 Increases E2F1 Protein, Induces Phosphorylation of STAT3 and Apoptosis

Molecules ◽

10.3390/molecules25235728 ◽

2020 ◽

Vol 25 (23) ◽

pp. 5728

Author(s):

Jensen M. Spear ◽

Zhixin Lu ◽

Wade A. Russu

Keyword(s):

Breast Cancer ◽

Colon Cancer ◽

Cell Viability ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Triple Negative Breast Cancer ◽

Triple Negative ◽

Colon Cancer Cell ◽

Colon Cancer Cell Lines

Cyclin-dependent kinase 8 (CDK8) has been identified as a colon cancer oncogene. Since this initial observation, CDK8 has been implicated as a potential driver of other cancers including acute myelogenous leukemia (AML) and some breast cancers. Here, we observed different biological responses to CDK8 inhibition among colon cancer cell lines and the triple-negative breast cancer (TNBC) cell line MDA-MB-468. When treated with CDK8 inhibitor 4, all treated cell lines responded with decreased cell viability and increased apoptosis. In the MDA-MB-468 cell line, the decrease in cell viability was dependent on increased phosphorylation of signal transducer and activator of transcription 3 (STAT3), which is not observed in the colon cancer cell lines. Furthermore, increased STAT3 phosphorylation in 4 treated MDA-MB-468 cells was dependent on increased transcription factor E2F1 protein. These results are consistent with previous reports of exogenous expression of E2F1-induced apoptosis in MDA-MB-468 cells.

Download Full-text