In this work, TiO2nanoparticles (NPs) were prepared through a well-developed hydrothermal method. The dry products were characterized by digital camera, transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermogravimetric method (TGA). These results suggested that TiO2NPs were well-crystallized and oleic acid-capped (OA-capped; 33.42 wt.%). In order to investigate the flocculation and stability of TiO2NPs colloids, redispersed experiments were carried out by altering the preparative parameters (e.g., redispersion solvent, washing times). In addition, the compounding stability of TiO2NPs colloids with AuNPs colloids and MnO4-solution under processing conditions (e.g., aging with stirring and under UV irradiation) were also studied. In principle, systematic investigations of flocculation and stability of TiO2NPs will be useful for their potential applications including novel photocatalysts and biosensors.