Applying PICRUSt and 16S rRNA functional characterisation to predicting co-digestion strategies of various animal manures for biogas production

An estimated 25 million tons of animal manure is produced globally every year, causing considerable impact to the environment. These impacts can be managed through the use of anaerobic digestion (AD) This process achieves waste degradation through enzymatic activity, the efficiency of the AD process is directly related to microorganisms that produce these enzymes. Biomethane potential (BMP) assays remain the standard theoretical framework to pre-determine biogas yield and have been used to determine the feasibility of substrates or their combination for biogas production. However, an integrated approach that combines substrate choice and co-digestion would provide an improvement to the current predictive models. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) addresses the limitations of assays in this regard. In this paper, the biochemical functions of horse, cow, and pig manures are predicted. A total of 135 predicted KEGG Orthologies (KOs) showed amino acids, carbohydrate, energy, lipid, and xenobiotic metabolisms in all the samples. Linear discriminant analysis (LDA) combined with the effect size measurements (LEfSe), showed that fructose, mannose, amino acid and nucleotide sugar, phosphotransferase (PST) as well as starch and sucrose metabolisms were significantly higher in horse manure samples. 36 of the KOs were related to the acidogenesis and/or acetogenesis AD stages. Extended bar plots showed that 11 significant predictions were observed for horse-cow, while 5 were predicted for horse-pig and for cow-pig manures. Based on these predictions, the AD process can be enhanced through co-digestion strategies that takes into account the predicted metabolic contributions of the manure samples. The results supported the BMP calculations for the samples in this study. Biogas yields can be improved if this combined approach is employed in routine analysis before co-digesting different substrates.

Shoot Production and Mineral Nutrients of Five Microgreens as Affected by Hydroponic Substrate Type and Post-Emergent Fertilization

As a new specialty crop with high market value, microgreens are vegetable or herb seedlings consumed at a young age, 7–21 days after germination. They are known as functional food with high concentrations of mineral nutrients and health beneficial phytochemicals. Microgreen industry lacks standardized recommendations on cultural practices including species/variety selection, substrate choice, and fertilization management. This study evaluated shoot growth and mineral nutrient concentrations in five microgreens including four Brassica and one Raphanus microgreens as affected by four hydroponic pad types and post-emergent fertilization in two experiments in January and February 2020. The five microgreens varied in their shoot height, fresh, dry shoot weights, and mineral nutrient concentrations with radish producing the highest fresh and dry shoot weights. Radish had the highest nitrogen (N) concentration and mustard had the highest phosphorus (P) concentrations when grown with three hydroponic pads except for hemp mat. Hydroponic pad type altered fresh, dry shoot weights, and mineral nutrients in tested microgreens. Microgreens in hemp mat showed the highest shoot height, fresh, dry shoot weights, and potassium (K) concentration, but the lowest N concentration in one or two experiments. One time post-emergent fertilization generally increased shoot height, fresh, dry shoot weights, and macronutrient concentrations in microgreens.

Subfamily Anischiinae (Coleoptera: Eucnemidae) in Early Cretaceous of Northeast China

Rheanischia new genus, type species Rheanischia brevicornis new species (Eucnemidae, Anischiinae) is described from the Lower Cretaceous of Liaoning, China. The presence of this species in early Cretaceous deposits provides new insight into the evolution of basal lignicolous Eucnemidae clades. Both Anischiinae and Palaeoxeninae species diversified in a world dominated by gymnosperms, before the main radiation of angiosperms. More than 95% of modern eucnemid larvae have a Palaeoxenus-type highly modified head structure, but contrary to the Palaeoxenus larva, they develop in angiosperm wood. Anischiinae utilize angiosperms as well, but their head capsule shows no such modifications. These facts prove that highly specialized morphological features do not offer definite proof of similar way of life in the distant past, nor should non-modified structures be taken as proof for another kind of substrate choice. Eucnemidae have invaded angiosperms with two quite different morphological adaptations. This fact may have implications for the evolution of all clicking elateroids.

Record of Anthophora (Clisodon) terminalis in a wooden trap-nesting block and comparison to available nesting information (Hymenoptera: Apidae)

Bee nesting substrate choice can influence habitat use, conservation effort efficacy, and population or landscape-use modeling, but information on nesting sites are often scattered in the literature. Here we bring together the available information on nests of a widespread bee, Anthophora (Clisodon) terminalis Cresson, and describe an unusual new nesting substrate use for this species.

Alternative splicing of COQ-2 determines the choice between ubiquinone and rhodoquinone biosynthesis in helminths

Parasitic helminths use two benzoquinones as electron carriers in the electron transport chain. In aerobic environments they use ubiquinone (UQ) but in anaerobic environments inside the host, they require rhodoquinone (RQ) and greatly increase RQ levels. The switch to RQ synthesis is driven by substrate selection by the polyprenyltransferase COQ-2 but the mechanisms underlying this substrate choice are unknown. We found that helminths make two coq-2 isoforms, coq-2a and coq-2e, by alternative splicing. COQ-2a is homologous to COQ2 from other eukaryotes but the COQ-2e-specific exon is only found in species that make RQ and its inclusion changes the enzyme core. We show COQ-2e is required for RQ synthesis and for survival in cyanide in C. elegans. Crucially, we see a switch from COQ-2a to COQ-2e as parasites transition into anaerobic environments. We conclude that under anaerobic conditions helminths switch from UQ to RQ synthesis via alternative splicing of coq-2.