Evaluation of three different concentration and extraction methods for recovery efficiency of human adenovirus and human rotavirus virus A

Biorefinery of Rubber Seed Kernels for Comprehensive Utilisation

10.21203/rs.3.rs-135640/v1 ◽

2020 ◽

Author(s):

Miao Yang ◽

Wenlei Zhu ◽

Hui Cao

Keyword(s):

Oil Recovery ◽

Protein Extraction ◽

Protein Denaturation ◽

Extraction Methods ◽

Alkaline Treatment ◽

Protein Recovery ◽

Recovery Efficiency ◽

Rubber Seed ◽

Rubber Production ◽

Comprehensive Utilization

Abstract Rubber seeds are a by-product of rubber production that is rich in oil and protein. Upgrading of rubber seeds to produce proteins, oils and feedstock can generate additional revenue for rubber production and reduce waste. The present study investigates the effects of different pre-treatments and extraction methods to determine the optimal methods to produce oil and protein from rubber seed kernels. Mechanical expulsion using a screw press and solvent extraction using n-hexane were employed for oil separation. The highest oil recovery efficiency of 95% was obtained using rubber seed meal that was pre-dried at 105 ℃. The sequential water-alkaline treatment was ideal for achieving high protein recovery while reducing the protein denaturation that can result from high operating temperatures and organic solvent contact. Over 90% of the total protein from rubber seed kernels could be recovered. Separating oil from kernels using hexane followed by protein extraction from the meals by enzymatic treatment provides a suitable method for comprehensive utilization of rubber seeds.

Download Full-text

Quantitative Detection of Human Adenovirus and Human Rotavirus Group A in Wastewater and El-Rahawy Drainage Canal Influencing River Nile in the North of Giza, Egypt

Food and Environmental Virology ◽

10.1007/s12560-020-09429-x ◽

2020 ◽

Vol 12 (3) ◽

pp. 218-225

Author(s):

Elmahdy M. Elmahdy ◽

Mohamed N. F. Shaheen ◽

Neveen M. Rizk ◽

Amal Saad-Hussein

Keyword(s):

Human Adenovirus ◽

Quantitative Detection ◽

River Nile ◽

Drainage Canal ◽

The North ◽

Human Rotavirus ◽

Group A

Download Full-text

Removal of pathogenic human viruses by insoluble pyridinium-type resin

Epidemiology and Infection ◽

10.1017/s0950268800048275 ◽

1990 ◽

Vol 105 (3) ◽

pp. 633-642 ◽

Cited By ~ 11

Author(s):

N. Kawabata ◽

K. Yamazaki ◽

T. Otake ◽

I. Oishi ◽

Y. Minekawa

Keyword(s):

Aqueous Solution ◽

Human Immunodeficiency Virus ◽

Continuous Flow ◽

Human Adenovirus ◽

Column Method ◽

Human Rotavirus ◽

Immunodeficiency Virus ◽

Removal Experiments ◽

Simplex Virus ◽

Human Viruses

SUMMARYCross-linked poly(N-benzyl-4-vinylpyridinium bromide) (BVP resin) was found to be very efficient in removing pathogenic human viruses from aqueous solution. In batch removal experiments using 50 g/1 of BVP resin at 35 °C. the level of infectivity in suspensions of enterovirus. herpes simplex virus, poliovirus, and human immunodeficiency virus was reduced 1000–100000 fold during a 2 h period. Those of coxsackievirus and echovirus were reduced 60–600 fold during 1 h contact. The haemagglutination titres of solutions of human rotavirus, influenza virus, human adenovirus. and Japanese encephalitis virus were reduced 16–256 fold during 30 min of contact. In removal experiments by a continuous flow column method for poliovirus. enterovirus. and coxsackievirus with initial infectivities of less than 105/ml, the infectivity of these viruses was no longer detectable in the effluent solution. For poliovirus, coxsackievirus, and echovirus with initial infectivities higher than 106, 99·8–99·9998% of the input viruses was removed as indicated by the reduction of infectivity.

Download Full-text

Influence of extraction methods on bioactive compounds from Ngoc Linh ginseng callus

Food Research ◽

10.26656/fr.2017.5(3).590 ◽

2021 ◽

Vol 5 (3) ◽

pp. 334-341

Author(s):

M.D. Lieu ◽

T.T.L. Nguyen ◽

T.H. Nguyen ◽

T.K.T. Dang ◽

D.G. Do

Keyword(s):

Bioactive Compounds ◽

Economic Value ◽

Power Saving ◽

Total Content ◽

Extraction Methods ◽

Recovery Efficiency ◽

Extraction Techniques ◽

Environmental Friendliness ◽

Saponin Content ◽

First Time

Panax vietnamensis Ha et Grushv or Vietnamese ginseng is a valuable medicinal herb with high economic value in the world. In this study, three target compounds were surveyed as polyphenols, polysaccharides, and saponins to evaluate the effects of factors including microwave, ultrasound, enzyme amylase, and cellulase for the first time. The results of the study showed that with different extraction conditions, the recovery efficiency of polyphenol, polysaccharide, and saponin was also different. With the addition of 1.2% of the enzyme amylase (v/v) in 8 hrs of incubation, the highest total content of polyphenol and polysaccharide was obtained, equivalent to 6.48 mg GAE/g sample and 312.48 mg Glu/g sample. While the total saponin content reached the highest value of 4.60 mg/g sample at 4 hrs of incubation using 0.8% of cellulase enzyme (v/v). The effect of microwave and ultrasound also showed a significant recovery efficiency for the three analyzed compounds. But compared to the use of two enzymes (amylase, cellulase), the efficiency was lower. The use of these extraction techniques was based on the advantages of environmental friendliness, simple operation, lower investment costs, and power saving. At the same time, it can improve the extraction and recovery efficiency as well as the activity of bioactive compounds.

Download Full-text

Biorefinery methods for extraction of oil and protein from rubber seed

Bioresources and Bioprocessing ◽

10.1186/s40643-021-00386-2 ◽

2021 ◽

Vol 8 (1) ◽

Author(s):

Miao Yang ◽

Wenlei Zhu ◽

Hui Cao

Keyword(s):

Oil Recovery ◽

Protein Extraction ◽

Protein Denaturation ◽

Extraction Methods ◽

Alkaline Treatment ◽

Protein Recovery ◽

Recovery Efficiency ◽

Rubber Seed ◽

Rubber Production ◽

Comprehensive Utilization

AbstractRubber seeds are a by-product of rubber production and are rich in oil and protein. Upgrading of rubber seeds to produce proteins, oils and feedstock can generate additional revenue for rubber production and reduce waste. The present study investigates the effects of different pre-treatments and extraction methods to determine the optimal methods to produce oil and protein from rubber seed kernels. Mechanical expulsion using a screw press and solvent extraction using n-hexane were employed for oil separation. The highest oil recovery efficiency of 95.12% was obtained using rubber seed meal that was pre-dried at 105 ℃. The sequential water–alkaline treatment was ideal for achieving high protein recovery while reducing the protein denaturation that can result from high operating temperatures and organic solvent contact. Over 90% of the total protein from rubber seed kernels could be recovered. Separating oil from kernels using hexane followed by protein extraction from the meals by enzymatic treatment provides a suitable method for comprehensive utilization of rubber seeds.

Download Full-text

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094607 ◽

1972 ◽

Vol 30 ◽

pp. 268-269

Author(s):

D. G. Chase ◽

W. Winters ◽

L. Piko

Keyword(s):

Hela Cells ◽

Human Adenovirus ◽

Mouse Embryos ◽

Equilibrium Density ◽

Cell Stage ◽

Virus Attachment ◽

Preimplantation Mouse Embryos ◽

Embryo Culture Medium ◽

Preimplantation Mouse ◽

Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

Download Full-text