Gonadotropin-inhibiting hormone promotes apoptosis of bovine ovary granulosa cells

The present study was undertaken to investigate the mRNA expression for gonadotropin-releasing hormone (GnRH) receptor in bovine ovary. Granulosa cells from small (< 4 mM), medium (5–8 mM) and large follicles (> 8 mM) and tissues from corpora lutea (CL) of different stages: Stage I (days 1–4), Stage II (days 5–10), Stage III (days 11–17), and Stage IV (days 18–21, days after ovulation) were harvested from bovine ovaries collected at a local abattoir. The mRNA isolated from representative samples was subjected to reverse transcription-polymerase chain reaction (RT-PCR) using gene sequence specific primers. The resultant PCR amplified gonadotropin-releasing horm one receptor (GnRH-R) cDNA products were identified and confirmed through Southern blot hybridization and nucleotide sequence analysis, respectively. The results showed the presence of GnRH-R mRNA transcripts in both follicles and CL. Key words: Bovine, granulosa cells, corpus luteum, GnRH receptor, mRNA

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Expression of Cyclins and Cyclin-Dependent Kinase Inhibitors in Granulosa Cells from Bovine Ovary

Reproduction in Domestic Animals ◽

10.1111/rda.12177 ◽

2013 ◽

Vol 48 (5) ◽

pp. e65-e69 ◽

Cited By ~ 16

Author(s):

T Shimizu ◽

Y Hirai ◽

A Miyamoto

Keyword(s):

Granulosa Cells ◽

Kinase Inhibitors ◽

Cyclin Dependent Kinase ◽

Bovine Ovary

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Hormonal regulation and differential expression of neuropilin (NRP)-1 and NRP-2 genes in bovine granulosa cells

Reproduction ◽

10.1530/rep.1.00937 ◽

2006 ◽

Vol 131 (3) ◽

pp. 555-559 ◽

Cited By ~ 11

Author(s):

Takashi Shimizu ◽

Barana C Jayawardana ◽

Hiromi Nishimoto ◽

Etsushi Kaneko ◽

Masafumi Tetsuka ◽

...

Keyword(s):

Granulosa Cells ◽

Hormonal Regulation ◽

Culture Medium ◽

Follicle Development ◽

Vascular Endothelial ◽

Vegf Receptors ◽

Theca Cells ◽

Bovine Ovary ◽

Endothelial Growth Factor

Although much is known about the biology of vascular endothelial growth factor (VEGF) and its receptors, little is known about the role of the VEGF receptors neuropilin (NRP)-1 and NRP-2 in the process of bovine follicle development. The aim of the present study was to examine the hormonal regulation of NRP-1 and NRP-2 mRNAs by real-time PCR in follicles from the bovine ovary and in cultured granulosa cells. The NRP-1 gene was expressed in the granulosa and theca cells in the post-selection (POF) and pre-selection (PRF) follicles in the bovine ovary. In contrast, the NRP-2 gene was expressed only in the theca cells in the POF and the PRF. The level of NRP-1 mRNA was significantly increased by treatment of the cultured granulosa cells with 10 ng/ml estradiol (E2). In contrast, the addition of progesterone (P4) to the culture medium decreased the expression of the NRP-1 gene. The level of NRP-1 mRNA was increased by 10 ng/ml E2 with or without 1 ng/ml P4, but the level of NRP-1 mRNA was decreased if the P4 level was increased to 10 ng/ml, even when 1 ng/ml E2 was also added. Follicle-stimulating hormone did not stimulate the expression of the NRP-1 gene. These results are the first data showing that NRP-1, but not NRP-2, is expressed in the granulosa cells of bovine follicles and that NRP-1 gene expression is regulated by sex steroids. Our findings suggest the involvement of NRP-1 in follicle development in the cow.

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Changes in the Proliferation/Apoptosis Balance in the Bovine Ovary: A Key Early Event in Follicular Persistence

Cells Tissues Organs ◽

10.1159/000481167 ◽

2017 ◽

Vol 204 (5-6) ◽

pp. 314-325 ◽

Cited By ~ 3

Author(s):

E. Matías Belotti ◽

Antonela F. Stassi ◽

Melisa M.L. Velázquez ◽

Pablo U. Díaz ◽

Belkis E. Marelli ◽

...

Keyword(s):

Cell Proliferation ◽

Granulosa Cells ◽

Caspase 3 ◽

Control Group ◽

Early Event ◽

Ki 67 ◽

Antral Follicles ◽

Theca Cells ◽

Bovine Ovary ◽

Bcl2 Expression

The objective of this work was to evaluate proliferation and apoptosis in the bovine ovary in a model of follicular persistence induced by low levels of progesterone to detect incipient changes during cystic ovarian disease development on the expected day of ovulation (day 0) and after 5, 10, and 15 days of follicular persistence. We analyzed cell proliferation by evaluating the expression of Ki-67 and apoptosis by evaluating caspase-3, BAX, and BCL2 expression. Proliferation was similar in the granulosa and theca cells of antral follicles in the P0 group (treated with progesterone up to the expected day of ovulation) and in the control group. A decrease in cell proliferation was detected after 5 days of persistence (P5) in relation to P0 (p < 0.05). Similar changes were found in the granulosa cells of the persistent follicles in relation to the control group (p < 0.05). Caspase-3 expression was similar in granulosa cells of antral follicles at early stages of persistence, with an increase after 15 days of persistence (p < 0.05). In the granulosa cells of group P10 (10 days of persistence), caspase-3 expression was reduced relative to that of antral follicles from the control group (p < 0.05). BCL2 expression was higher in granulosa cells of the persistent follicles of group P0 relative to the control follicles, with no changes in BAX expression, which was increased in persistent follicles of group P15 (p < 0.05). Similar results were observed in theca cells at initial stages of persistence. The results show that, initially, proliferation is maintained with low apoptosis and an increase in cell survival.

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