Redox status and oxidative stress during late pregnancy and postpartum period in mares

2019 ◽  
Vol 230 ◽  
pp. 103821 ◽  
Author(s):  
Stefano Cecchini ◽  
Francesco Fazio ◽  
Marilena Bazzano ◽  
Anna Rocchina Caputo ◽  
Claudia Giannetto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Postpartum Period ◽  
Late Pregnancy ◽  
Redox Status ◽  
Pregnancy And Postpartum ◽  
And Oxidative Stress

scholarly journals Paraoxonase activities and oxidative status during late pregnancy and postpartum period in dairy cattle with and without retained fetal membrane

2018 ◽  
Vol 68 (1) ◽  
pp. 45
Author(s):  
M. DURAK ◽  
B. YOKUS ◽  
N. ERCAN
Keyword(s):  
Oxidative Stress ◽  
Postpartum Period ◽  
Late Pregnancy ◽  
Blood Parameters ◽  
Observation Point ◽  
Paraoxonase 1 ◽  
Stress Index ◽  
Pon1 Activity ◽  
Fetal Membrane ◽  
Pregnancy And Postpartum

The aim of this study was to evaluate serum paraoxonase 1 (PON1) activities, total antioxidant capacity (TAC), total peroxidation (TPX), oxidative stress index (OSI) and their associations with Retained fetal membrane (RFM) in late pregnancy and postpartum period. Possible relationships have been investigated between these markers and other relevant blood parameters also. Totally 266 pregnant cows were included in this study. Samples were taken in during late pregnancy and postpartum periods. The cows were divided into two groups after giving birth as RFM and Non-Retained fetal membrane (NRFM). The TAC, TPX and OSI values were not different in RFM compared to NRFM in both periods. The PON1 activities of RFM group in both periods were lower than those of the NRFM, however; these variations were not statistically significant. PON1 activities was statistically higher in the late pregnancy both RFM and NRFM groups than postpartum. This observation point out oxidative stress could not relate to pathogenesis of RFM. The PON1 activity was increased physiologically in pregnant cows, and more information is needed to determine whether PON1 may be used to identify cows at high risk of developing RFM. Decreasing serum urea/creatinine ratio, globulin and total protein concentration and increasing albumin/globulin ratio might be a parameter to contributing use in diagnosis of RFM.

Uncovering the beginning of diabetes: the cellular redox status and oxidative stress as starting players in hyperglycemic damage

2013 ◽  
Vol 376 (1-2) ◽  
pp. 103-110 ◽  
Author(s):  
João Soeiro Teodoro ◽  
Ana Patrícia Gomes ◽  
Ana Teresa Varela ◽  
Filipe Valente Duarte ◽  
Anabela Pinto Rolo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Redox Status ◽  
Cellular Redox ◽  
And Oxidative Stress

Quercetin protects rat hepatocytes from oxidative damage induced by ethanol and iron by maintaining intercellular liable iron pool

2013 ◽  
Vol 33 (5) ◽  
pp. 534-541 ◽  
Author(s):  
Y Li ◽  
Y Deng ◽  
Y Tang ◽  
H Yu ◽  
C Gao ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Iron Overload ◽  
Combined Treatment ◽  
Rat Hepatocytes ◽  
Redox Status ◽  
Glutathione Depletion ◽  
Labile Iron Pool ◽  
Iron Pool ◽  
And Oxidative Stress

Accumulating evidence has shown that ethanol-induced iron overload plays a crucial role in the development and progression of alcoholic liver disease. We designed the present study to investigate the potential protective effect of quercetin, a naturally occurring iron-chelating antioxidant on alcoholic iron overload and oxidative stress. Ethanol-incubated (100 mmol/L) rat primary hepatocytes were co-treated by quercetin (100 µmol/L) and different dose of ferric nitrilotriacetate (Fe-NTA) for 24 h. When the hepatic enzyme releases in the culture medium, redox status of hepatocytes and the intercellular labile iron pool (LIP) level were assayed. Our data showed that Fe-NTA dose dependently induced cellular leakage of aspartate transaminase and lactate dehydrogenase, glutathione depletion, superoxide dismutase inactivation, and overproduction of malondialdehyde) and reactive oxygen species (ROS) of intact and especially ethanol-incubated hepatocytes. The oxidative damage resulted from ethanol, Fe-NTA, and especially their combined treatment was substantially alleviated by quercetin, accompanying the corresponding normalization of intercellular LIP level. Iron in excess, thus, may aggravate ethanol hepatotoxicity through Fenton-active LIP, and quercetin attenuated ethanol-induced iron and oxidative stress. To maintain intercellular LIP contributes to the hepatoprotective effect of quercetin besides its direct ROS-quenching activity.

scholarly journals Glycine Increases Insulin Sensitivity and Glutathione Biosynthesis and Protects against Oxidative Stress in a Model of Sucrose-Induced Insulin Resistance

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Mohammed El-Hafidi ◽  
Martha Franco ◽  
Angélica Ruiz Ramírez ◽  
José Santamaria Sosa ◽  
José Antonio Pineda Flores ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Insulin Sensitivity ◽  
Insulin Receptor ◽  
Redox Status ◽  
Glutathione Biosynthesis ◽  
Stress Markers ◽  
Glutamylcysteine Synthetase ◽  
Key Enzyme ◽  
And Oxidative Stress

Oxidative stress and redox status play a central role in the link between insulin resistance (IR) and lipotoxicity in metabolic syndrome. This mechanistic link may involve alterations in the glutathione redox state. We examined the effect of glycine supplementation to diet on glutathione biosynthesis, oxidative stress, IR, and insulin cell signaling in liver from sucrose-fed (SF) rats characterized by IR and oxidative stress. Our hypothesis is that the correction of glutathione levels by glycine treatment leads to reduced oxidative stress, a mechanism associated with improved insulin signaling and IR. Glycine treatment decreases the levels of oxidative stress markers in liver from SF rats and increases the concentrations of glutathione (GSH) and γ-glutamylcysteine and the amount of γ-glutamylcysteine synthetase (γ-GCS), a key enzyme of GSH biosynthesis in liver from SF rats. In liver from SF rats, glycine also decreases the insulin-induced phosphorylation of insulin receptor substrate-1 (ISR-1) in serine residue and increases the phosphorylation of insulin receptor β-subunit (IR-β) in tyrosine residue. Thus, supplementing diets with glycine to correct GSH deficiency and to reduce oxidative stress provides significant metabolic benefits to SF rats by improving insulin sensitivity.

scholarly journals Extensive Thiol Profiling for Assessment of Intracellular Redox Status in Cultured Cells by HPLC-MS/MS

Antioxidants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 24
Author(s):  
Jiandong Wu ◽  
Anna Chernatynskaya ◽  
Annalise Pfaff ◽  
Huari Kou ◽  
Nan Cen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Responses ◽  
Defense Mechanisms ◽  
High Performance ◽  
Cultured Cells ◽  
Redox Status ◽  
Oxidative Stress Responses ◽  
Sulfur Containing ◽  
And Oxidative Stress ◽  
Oxidative Insult

Oxidative stress may contribute to the pathology of many diseases, and endogenous thiols, especially glutathione (GSH) and its metabolites, play essential roles in the maintenance of normal redox status. Understanding how these metabolites change in response to oxidative insult can provide key insights into potential methods of prevention and treatment. Most existing methodologies focus only on the GSH/GSH disulfide (GSSG) redox couple, but GSH regulation is highly complex and depends on several pathways with multiple redox-active sulfur-containing species. In order to more fully characterize thiol redox status in response to oxidative insult, a high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) method was developed to simultaneously determine seven sulfur-containing metabolites, generating a panel that systematically examines several pathways involved in thiol metabolism and oxidative stress responses. The sensitivity (LOQ as low as 0.01 ng/mL), accuracy (88–126% spike recovery), and precision (≤12% RSD) were comparable or superior to those of existing methods. Additionally, the method was used to compare the baseline thiol profiles and oxidative stress responses of cell lines derived from different tissues. The results revealed a previously unreported response to oxidative stress in lens epithelial (B3) cells, which may be exploited as a new therapeutic target for oxidative-stress-related ocular diseases. Further application of this method may uncover new pathways involved in oxidative-stress-related diseases and endogenous defense mechanisms.

scholarly journals The Importance of Redox Status in the Frame of Lifestyle Approaches and the Genetics of the Lung Innate Immune Molecules, SP-A1 and SP-A2, on Differential Outcomes of COVID-19 Infection

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 784 ◽  
Author(s):  
Fotios Tekos ◽  
Zoi Skaperda ◽  
Nikolaos Goutzourelas ◽  
David S. Phelps ◽  
Joanna Floros ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protein A ◽  
The Elderly ◽  
Redox Balance ◽  
Surfactant Protein ◽  
Redox Status ◽  
Innate Immune ◽  
Differential Outcomes ◽  
Underlying Diseases ◽  
And Oxidative Stress

The pandemic of COVID-19 is of great concern to the scientific community. This mainly affects the elderly and people with underlying diseases. People with obesity are more likely to experience unpleasant disease symptoms and increased mortality. The severe oxidative environment that occurs in obesity due to chronic inflammation permits viral activation of further inflammation leading to severe lung disease. Lifestyle affects the levels of inflammation and oxidative stress. It has been shown that a careful diet rich in antioxidants, regular exercise, and fasting regimens, each and/or together, can reduce the levels of inflammation and oxidative stress and strengthen the immune system as they lead to weight loss and activate cellular antioxidant mechanisms and reduce oxidative damage. Thus, a lifestyle change based on the three pillars: antioxidants, exercise, and fasting could act as a proactive preventative measure against the adverse effects of COVID-19 by maintaining redox balance and well-functioning immunity. Moreover, because of the observed diversity in the expression of COVID-19 inflammation, the role of genetics of innate immune molecules, surfactant protein A (SP-A)1 and SP-A2, and their differential impact on the local lung microenvironment and host defense is reviewed as genetics may play a major role in the diverse expression of the disease.

scholarly journals The Universal Soldier: Enzymatic and Non-Enzymatic Antioxidant Functions of Serum Albumin

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 966
Author(s):  
Daria A. Belinskaia ◽  
Polina A. Voronina ◽  
Vladimir I. Shmurak ◽  
Mikhail A. Vovk ◽  
Anastasia A. Batalova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Serum Albumin ◽  
Antioxidant Properties ◽  
Redox Status ◽  
Allosteric Modulation ◽  
Biologically Active ◽  
Redox Modulation ◽  
Antioxidant Defence ◽  
First Time ◽  
And Oxidative Stress

As a carrier of many biologically active compounds, blood is exposed to oxidants to a greater extent than the intracellular environment. Serum albumin plays a key role in antioxidant defence under both normal and oxidative stress conditions. This review evaluates data published in the literature and from our own research on the mechanisms of the enzymatic and non-enzymatic activities of albumin that determine its participation in redox modulation of plasma and intercellular fluid. For the first time, the results of numerous clinical, biochemical, spectroscopic and computational experiments devoted to the study of allosteric modulation of the functional properties of the protein associated with its participation in antioxidant defence are analysed. It has been concluded that it is fundamentally possible to regulate the antioxidant properties of albumin with various ligands, and the binding and/or enzymatic features of the protein by changing its redox status. The perspectives for using the antioxidant properties of albumin in practice are discussed.

Effects of a 12-day maximal shuttle-run shock microcycle in hypoxia on soccer specific performance and oxidative stress

2015 ◽  
Vol 40 (8) ◽  
pp. 842-845 ◽  
Author(s):  
Hannes Gatterer ◽  
Kultida Klarod ◽  
Dieter Heinrich ◽  
Philipp Schlemmer ◽  
Stefan Dilitz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Test Performance ◽  
Redox Status ◽  
Soccer Players ◽  
Performance Improvements ◽  
Repeated Sprint ◽  
Specific Performance ◽  
Oxidative Stress Level ◽  
And Oxidative Stress ◽  
Shuttle Run

The purpose of this study was to investigate the effect of a maximal shuttle-run shock microcycle in hypoxia on repeated sprint ability (RSA, 6 × 40-m (6 × 20 m back and forth, 20″ rest in between)), Yo-Yo-intermittent-recovery (YYIR) test performance, and redox-status. Fourteen soccer players (age: 23.9 ± 2.1 years), randomly assigned to hypoxia (∼3300 m) or normoxia training, performed 8 maximal shuttle-run training sessions within 12 days. YYIR test performance and RSA fatigue-slope improved independently of the hypoxia stimulus (p < 0.05). Training reduced the oxidative stress level (−7.9%, p < 0.05), and the reduction was associated with performance improvements (r = 0.761, ΔRSA; r = −0.575, ΔYYIR, p < 0.05).

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