(−)-Epigallocatechin gallate protected molecular structure of collagen fibers in sea cucumber Apostichopus japonicus body wall during thermal treatment

ABSTRACTSea cucumbers Apostichopus japonicus (3.54 ± 0.01 g of wet weight) were exposed to five concentrations of dietary hexavalent chromium [0 (control), 100, 200, 400, and 800 mg Cr6+/kg dry weight] amended with K2Cr2O7 for 30 days. The bioaccumulation and immune responses [antioxidant enzymes: superoxide dismutase (SOD) and catalase (CAT); hydrolytic enzymes: acid phosphatase (ACP) and alkaline phosphatase (AKP)] of sea cucumbers were subsequently evaluated. This study found that the order of Cr accumulation in the experimental tissues was respiratory tree > intestine > body wall. Significantly lower SOD activities occurred in the 400 mg/kg group compared to that in the control group. Higher dietary Cr6+ exposure (400 and 800 mg Cr6+ /kg dry weight) did not negatively alter the CAT activities, but significantly inhibited CAT activities in 100 mg/kg group, compared to control group. ACP activities in groups 200, 400 and 800 mg/kg were significantly lower than those in control group, while no significant differences occurred in AKP activities among groups. The present study provides important information into the bioaccumulation and immune responses of the sea cucumber A. japonicus in response to chronic dietary Cr6+ exposure.

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Fucosylated chondroitin sulfate is covalently associated with collagen fibrils in sea cucumber Apostichopus japonicus body wall

Carbohydrate Polymers ◽

10.1016/j.carbpol.2018.01.041 ◽

2018 ◽

Vol 186 ◽

pp. 439-444 ◽

Cited By ~ 11

Author(s):

Jun Wang ◽

Yaoguang Chang ◽

Fanxiu Wu ◽

Xiaoqi Xu ◽

Changhu Xue

Keyword(s):

Chondroitin Sulfate ◽

Sea Cucumber ◽

Body Wall ◽

Apostichopus Japonicus ◽

Collagen Fibrils

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Characterization and phylogenetic analysis of lectin gene cDNA isolated from sea cucumber (Apostichopus japonicus) body wall

Journal of Ocean University of China ◽

10.1007/s11802-017-3363-z ◽

2017 ◽

Vol 16 (6) ◽

pp. 1148-1156

Author(s):

Zhuang Xue ◽

Hui Li ◽

Yang Liu ◽

Wei Zhou ◽

Jing Sun ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Sea Cucumber ◽

Body Wall ◽

Apostichopus Japonicus ◽

Lectin Gene

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Fatty acid component in sea cucumber Apostichopus japonicus from different tissues and habitats

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s002531541500168x ◽

2015 ◽

Vol 96 (1) ◽

pp. 197-204 ◽

Cited By ~ 4

Author(s):

Qinzeng Xu ◽

Qiang Xu ◽

Xuelei Zhang ◽

Quancai Peng ◽

Hongsheng Yang

Keyword(s):

Organic Matter ◽

Sea Cucumber ◽

Body Wall ◽

Apostichopus Japonicus ◽

The Body ◽

Food Sources ◽

Sea Cucumbers ◽

Protein Energy ◽

Strong Current ◽

Benthic Food

Fatty acids (FA) are a non-protein energy source and can act as trophic biomarkers in benthic food webs. We analysed the FA profiles of sea cucumber Apostichopus japonicus, comparing tissues of body wall, gut sediment and ovaries in two habitats. Rongcheng Bay: kelp raft cultivation area with high organic matter in sediment; Laoshan Bay: strong current with low sediment organic matter. The results showed that body wall and ovary tissues were rich in long chain polyunsaturated FA (LC-PUFA), which contributed ~31% to the FA dissimilarity between the two tissues. SIMPER (similarity percentages routine) results showed that C20:5ω3 (EPA), C18:1ω7, C20:4ω6 (AA), C16:0, C14:1 and C20:1ω11 contributed to dissimilarity between the body wall and ovary tissues, while 16:1ω7, 20:5ω3, C16:0, C18:1ω7, C18:0 and C14:1 contributed more to the dissimilarity of body wall tissues between the two habitats. FA biomarkers showed that sea cucumbers from the two habitats had different food sources, with brown kelp and vascular plants being the main food for sea cucumbers in Rongcheng and diatoms for those in Laoshan. To better understand differences in FA composition in sea cucumbers, more research is needed examining a wider diversity of tissue types and habitats.

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