Effect of immobilized Lactobacillus casei on the evolution of flavor compounds in probiotic dry-fermented sausages during ripening

Meat Science ◽  
2015 ◽  
Vol 100 ◽  
pp. 41-51 ◽  
Author(s):  
Marianthi Sidira ◽  
Panagiotis Kandylis ◽  
Maria Kanellaki ◽  
Yiannis Kourkoutas
Foods ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 219 ◽  
Author(s):  
Marianthi Sidira ◽  
Gregoria Mitropoulou ◽  
Alex Galanis ◽  
Maria Kanellaki ◽  
Yiannis Kourkoutas

The aim of the present study was to investigate the effect of sugar content (0, 0.30, and 0.60%) on quality attributes and shelf-life of dry-fermented sausages stored for 66 days containing free or immobilized Lactobacillus casei ATCC 393 on wheat. For comparison, dry-fermented sausages with no starter culture were also produced. Physicochemical characteristics ranged within the levels usually observed in fermented sausages, while a drastic decrease was recorded in numbers of enterobacteria, staphylococci, and pseudomonads during ripening in all cases. Noticeably, sugar addition and the probiotic culture resulted in significant increase of shelf-life, whereas levels of L. casei ATCC 393 after 66 days of ripening persisted above 6 log cfu/g. Sugar addition had a positive effect on sensory attributes; although all products were of high quality, the immobilized cells provided a distinctive characteristic aroma and a fine taste.


2016 ◽  
Vol 201 ◽  
pp. 334-338 ◽  
Author(s):  
Marianthi Sidira ◽  
Panagiotis Kandylis ◽  
Maria Kanellaki ◽  
Yiannis Kourkoutas

Molecules ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 223
Author(s):  
Lei Li ◽  
Carmela Belloch ◽  
Mónica Flores

Flavor is amongst the major personal satisfaction indicators for meat products. The aroma of dry cured meat products is generated under specific conditions such as long ripening periods and mild temperatures. In these conditions, the contribution of Maillard reactions to the generation of the dry cured flavor is unknown. The main purpose of this study was to examine mild curing conditions such as temperature, pH and aw for the generation of volatile compounds responsible for the cured meat aroma in model systems simulating dry fermented sausages. The different conditions were tested in model systems resembling dry fermented sausages at different stages of production. Three conditions of model system, labeled initial (I), 1st drying (1D) and 2nd drying (2D) and containing different concentrations of amino acid and curing additives, as well as different pH and aw values, were incubated at different temperatures. Changes in the profile of the volatile compounds were investigated by solid phase microextraction and gas chromatography mass spectrometry (SPME-GS-MS) as well as the amino acid content. Seventeen volatile compounds were identified and quantified in the model systems. A significant production of branched chain volatile compounds, sulfur, furans, pyrazines and heterocyclic volatile compounds were detected in the model systems. At the drying stages, temperature was the main factor affecting volatile production, followed by amino acid concentration and aw. This research demonstrates that at the mild curing conditions used to produce dry cured meat product volatile compounds are generated via the Maillard reaction from free amino acids. Moreover, in these conditions aw plays an important role promoting formation of flavor compounds.


Author(s):  
Radka Burdychová ◽  
P. Hoferková

Combination of microbiology cultivation methods and methods of molecular biology is the best way how to achieve correct qualitative and quantitative probiotic analysis. The aim of this study was mo­ni­to­ring of amounts of different starter bacteria supplemented with probiotic bacterial cells Lactobacillus casei (Chr. Hansen) during fermentation and ripening of fermented sausages. Two starter cultures, one consisted of Pediococcus pentosaceus and Staphylococcus xylosus, the second of Pediococcus pentosaceus and Staphylococcus carnosus, were used for the production of sausages. As a detection method of starter and probiotic cell counts, the cultivation on MRS –IM – sorbitol agar was used. Furthermore, the confirmation of L. casei was carried out using species specific PCR. The counts of probiotic L. casei in both probiotic sausages were 106/g and stayed at this level during the whole ripening period (100 days). The counts of starter bacteria were 107/g after the 7 days of fermentation and stayed at this level during the whole fermentation period. PCR from one bacterial colony confirmed the identity of L. casei in 80% of analysed colonies.


Food Control ◽  
2014 ◽  
Vol 42 ◽  
pp. 315-320 ◽  
Author(s):  
Marianthi Sidira ◽  
Alex Galanis ◽  
Anastasios Nikolaou ◽  
Maria Kanellaki ◽  
Yiannis Kourkoutas

2015 ◽  
Vol 178 ◽  
pp. 201-207 ◽  
Author(s):  
Marianthi Sidira ◽  
Panagiotis Kandylis ◽  
Maria Kanellaki ◽  
Yiannis Kourkoutas

Author(s):  
Cristina Serra-Castelló ◽  
Sara Bover-Cid ◽  
Margarita Garriga ◽  
Tina Beck Hansen ◽  
Annemarie Gunvig ◽  
...  

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