Hemodynamic resuscitation with fluids bolus and norepinephrine increases severity of lung damage in an experimental model of septic shock

2021 ◽  
Vol 45 (9) ◽  
pp. 532-540
Author(s):  
P. Guijo Gonzalez ◽  
M.A. Gracia Romero ◽  
A. Gil Cano ◽  
M. Garcia Rojo ◽  
M. Cecconi ◽  
...  
Keyword(s):  
Septic Shock ◽  
Experimental Model ◽  
Lung Damage

Hemodynamic resuscitation with fluids bolus and norepinephrine increases severity of lung damage in an experimental model of septic shock

2020 ◽  
Author(s):  
P. Guijo Gonzalez ◽  
M.A. Gracia Romero ◽  
A. Gil Cano ◽  
M. Garcia Rojo ◽  
M. Cecconi ◽  
...  
Keyword(s):  
Septic Shock ◽  
Experimental Model ◽  
Lung Damage

scholarly journals Igm-enriched polyclonal immunoglobulins in an experimental model of gram negative pneumonia developing septic shock

2015 ◽  
Vol 3 (S1) ◽  
Author(s):  
R Vaschetto ◽  
N Clemente ◽  
A Pagni ◽  
T Esposito ◽  
F Mercalli ◽  
...  
Keyword(s):  
Septic Shock ◽  
Experimental Model ◽  
Gram Negative

scholarly journals Antiseptic Effect of Ps-K18: Mechanism of Its Antibacterial and Anti-Inflammatory Activities

2019 ◽  
Vol 20 (19) ◽  
pp. 4895 ◽  
Author(s):  
Mihee Jang ◽  
Jieun Kim ◽  
Yujin Choi ◽  
JeongKyu Bang ◽  
Yangmee Kim
Keyword(s):  
Septic Shock ◽  
Antibacterial Activity ◽  
Bioactive Peptides ◽  
Liver Toxicity ◽  
Inflammatory Responses ◽  
Lung Damage ◽  
Peptide Antibiotic ◽  
Gram Negative ◽  
Innate Defense ◽  
Anti Inflammatory

Recently, bioactive peptides have attracted attention for their therapeutic applications in the pharmaceutical industry. Among them, antimicrobial peptides are candidates for new antibiotic drugs. Since pseudin-2 (Ps), isolated from the skin of the paradoxical frog Pseudis paradoxa, shows broad-spectrum antibacterial activity with high cytotoxicity, we previously designed Ps-K18 with a Lys substitution for Leu18 in Ps, which showed high antibacterial activity and low toxicity. Here, we examined the potency of Ps-K18, aiming to develop antibiotics derived from bioactive peptides for the treatment of Gram-negative sepsis. We first investigated the antibacterial mechanism of Ps-K18 based on confocal micrographs and field emission scanning electron microscopy, confirming that Ps-K18 targets the bacterial membrane. Anti-inflammatory mechanism of Ps-K18 was investigated by secreted alkaline phosphatase reporter gene assays and RT-PCR, which revealed that Ps-K18 activates innate defense via Toll-like receptor 4-mediated nuclear factor-kappa B signaling pathways. Moreover, we investigated the antiseptic effect of Ps-K18 using a lipopolysaccharide or Escherichia coli K1-induced septic shock mouse model. Ps-K18 significantly reduced bacterial growth and inflammatory responses in the septic shock model. Ps-K18 showed low renal and liver toxicity and attenuated lung damage effectively. This study suggests that Ps-K18 is a potent peptide antibiotic that could be applied therapeutically to Gram-negative sepsis.

Development of a septic shock experimental model oriented at training. Application in the training of depuration techniques in the management of severe sepsis

2011 ◽  
Vol 35 (2) ◽  
pp. 84-91
Author(s):  
M.E. Herrera-Gutiérrez ◽  
G. Seller-Pérez ◽  
G. Quesada García ◽  
M.M. Granados ◽  
J.M. Domínguez ◽  
...  
Keyword(s):  
Septic Shock ◽  
Severe Sepsis ◽  
Experimental Model

A comparison of the effect of convection against diffusion in hemodynamics and cytokines clearance in an experimental model of septic shock

2012 ◽  
Vol 73 (4) ◽  
pp. 855-860 ◽  
Author(s):  
Manuel E. Herrera-Gutiérrez ◽  
Gemma Seller-Pérez ◽  
Dolores Arias-Verdú ◽  
Maria M. Granados ◽  
Juan M. Dominguez ◽  
...  
Keyword(s):  
Septic Shock ◽  
Experimental Model

scholarly journals Study of Ionized Magnesium and Calcium Levels in a Cohort of Patients with Septic Shock Treated with Standard Therapy and Antioxidants

2021 ◽  
Author(s):  
Alfredo Aisa-Álvarez ◽  
María Elena Soto ◽  
Gilberto Camarena-Alejo ◽  
Juvenal Franco-Granillo ◽  
Randall Cruz Soto ◽  
...  
Keyword(s):  
Septic Shock ◽  
Myocardial Dysfunction ◽  
Group Versus ◽  
Serum Levels ◽  
Lung Damage ◽  
Control Group ◽  
Antioxidant Treatment ◽  
Standard Management ◽  
Before And After ◽  
Healthy Control

Abstract Background: Septic shock is the most serious form of sepsis and can be due to several factors, such as hypovolemia, vascular hyporesponsiveness, myocardial dysfunction, or dysfunction of the circulation. Likewise, electrolyte levels have been associated with septic shock in intensive care units, although it has been underdiagnosed. Based on this, the purpose of the present work was to evaluate plasma ionic levels in patients with septic shock before and after treatment with different antioxidants. Methods: Plasma ionic levels were measured (Na+, K+, Cl- and ionized Ca2+ and Mg2+) in 194 subjects, 129 healthy control patients, 14 patients with septic shock without treatment and 51 patients with septic shock under treatment with 4 different antioxidants (N-acetyl cysteine, melatonin, vitamin C and vitamin E). Results: We found important differences when comparing the plasma ionic levels of K+, Ca2+ and Mg2+ between the control group versus in both groups with sepsis at the time of hospital admission. In patients with septic shock, there is a decrease in the serum levels of ionized Na+, K+, Cl- and Ca2+ and Mg2+. Antioxidant treatment as an adjunct to the standard management of patients with septic shock increases the electrolyte deficit. The correction of the magnesium deficit also leads to an increase in serum calcium and potassium levels. Conclusion: The management of antioxidant therapy in patients with septic shock within the first hours of admission can help to improve their ionic levels of Ca2+ and Mg2+, mainly in patients with lung damage.Clinical Trial gov registration: NCT03557229. Registered june 14, 2018. https://clinicaltrials.gov/ct2/show/NTC03557229?term=aISA+ALFREDO&draw=2&rank=1

scholarly journals Iron chelates bind nitric oxide and decrease mortality in an experimental model of septic shock.

1996 ◽  
Vol 93 (17) ◽  
pp. 9138-9141 ◽  
Author(s):  
W. M. Kazmierski ◽  
G. Wolberg ◽  
J. G. Wilson ◽  
S. R. Smith ◽  
D. S. Williams ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Septic Shock ◽  
Experimental Model ◽  
Iron Chelates

scholarly journals Transduced PEP-1-Heme Oxygenase-1 Fusion Protein Attenuates Lung Injury in Septic Shock Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-12
Author(s):  
Xue-Tao Yan ◽  
Xiang-Hu He ◽  
Yan-Lin Wang ◽  
Zong-Ze Zhang ◽  
Jun-Jiao Tang
Keyword(s):  
Oxidative Stress ◽  
Septic Shock ◽  
Lung Injury ◽  
Fusion Protein ◽  
Heme Oxygenase ◽  
Cecal Ligation ◽  
Protective Role ◽  
Lung Damage ◽  
Heme Oxygenase 1 ◽  
Anti Inflammatory

Oxidative stress and inflammation have been identified to play a vital role in the pathogenesis of lung injury induced by septic shock. Heme oxygenase-1 (HO-1), an effective antioxidant and anti-inflammatory and antiapoptotic substance, has been used for the treatment of heart, lung, and liver diseases. Thus, we postulated that administration of exogenous HO-1 protein transduced by cell-penetrating peptide PEP-1 has a protective role against septic shock-induced lung injury. Septic shock produced by cecal ligation and puncture caused severe lung damage, manifested in the increase in the lung wet/dry ratio, oxidative stress, inflammation, and apoptosis. However, these changes were reversed by treatment with the PEP-1-HO-1 fusion protein, whereas lung injury in septic shock rats was alleviated. Furthermore, the septic shock upregulated the expression of Toll-like receptor 4 (TLR4) and transcription factor NF-κB, accompanied by the increase of lung injury. Administration of PEP-1-HO-1 fusion protein reversed septic shock-induced lung injury by downregulating the expression of TLR4 and NF-κB. Our study indicates that treatment with HO-1 protein transduced by PEP-1 confers protection against septic shock-induced lung injury by its antioxidant, anti-inflammatory, and antiapoptotic effects.

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