scholarly journals High prevalence of clade 8 Escherichia coli O157:H7 isolated from retail meat and butcher shop environment

2016 ◽  
Vol 45 ◽  
pp. 1-5 ◽  
Author(s):  
Lucía Galli ◽  
Victoria Brusa ◽  
Pallavi Singh ◽  
Angel Adrián Cataldi ◽  
Shannon Manning ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
High Prevalence ◽  
Retail Meat ◽  
Butcher Shop

Pulsed-Field Gel Electrophoresis Characterization of Shiga Toxin–Producing Escherichia coli O157 from Hides of Cattle at Slaughter

2002 ◽  
Vol 65 (7) ◽  
pp. 1172-1176 ◽  
Author(s):  
S. M. AVERY ◽  
A. SMALL ◽  
C.-A. REID ◽  
S. BUNCIC
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gel Electrophoresis ◽  
Immunomagnetic Separation ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Adult Cattle ◽  
Pulsed Field ◽  
E Coli ◽  
High Prevalence

Contamination of the brisket areas of the hides of healthy adult cattle with Shiga toxin–producing Escherichia coli O157 at slaughter in England was studied. In total, 73 cattle consignments comprising 584 animals delivered to one abattoir over 3 days during 1 week in July 2001 were studied: 26 cattle consignments arriving on Monday, 32 consignments arriving on Wednesday, and 15 consignments arriving on Friday. Consignment sizes ranged from 1 to 23 animals, with a mean consignment size of 8. The hide of the first animal to be slaughtered in each consignment was sampled by using a sponge swab moistened with 0.85% saline to rub an unmeasured brisket (ventral) area (ca. 30 by 30 cm). The process of isolating E. coli O157 from the swabs consisted of enrichment, screening with immunoprecipitation assay kits, and immunomagnetic separation. E. coli O157 was found on 24 of 73 (32.9%) cattle hides examined, and 21 of these 24 isolates produced Shiga toxins. The 24 E. coli O157 isolates produced six different pulsed-field gel electrophoresis profiles, and 18 (75%) of the isolates were of one prevalent clone. The high prevalence of one E. coli O157 clone on the hides of cattle at slaughter could be due to a high prevalence of that clone on the 18 farms involved (not investigated in the current study), in the postfarm transport or lairage environments, or both. Since the lairage environment, but not the farm of origin or the postfarm transport vehicle, was a factor common to all 18 cattle consignments, it could have played an important role in spreading the prevalent E. coli O157 clone to the cattle hides. Lairage pen floors and the stunning box floor were identified as the most probable sites along the unloading-to-slaughter route at which the brisket areas of cattle hides could become contaminated.

Effects of Farm Manure-Handling Practices on Escherichia coli O157 Prevalence in Cattle

1997 ◽  
Vol 60 (4) ◽  
pp. 363-366 ◽  
Author(s):  
DALE D. HANCOCK ◽  
DANIEL H. RICE ◽  
DONALD E. HERRIOTT ◽  
THOMAS E. BESSER ◽  
ERIC D. EBEL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Herds ◽  
Escherichia Coli O157 ◽  
Forage Crops ◽  
Fecal Samples ◽  
Herd Prevalence ◽  
E Coli ◽  
High Prevalence ◽  
Handling Practices ◽  
Culture Positive

Thirty-six dairy herds in Idaho, Oregon, and Washington were selected on the basis of cattle housing and manure-handling practices. Approximately 60 fecal samples from heifers were collected monthly in each herd for 6 months and cultured for Escherichia coli O157. One hundred seventy-nine of 12,664 (1.41%) individual fecal samples from 27 of the 36 herds (75%) were culture positive for E. coli O157. Within-herd prevalence ranged from 0% to 5.5% with a strong clustering toward the lower end of this range. A tendency was observed for herds to maintain either a relatively low or high prevalence of E. coli O157. Prevalence of E. coli O157 was similar in herds which housed heifers in dry lots and on pasture with and without application of manure. Also, application of manure to cattle forage crops was not associated with the prevalence of E. coli O157 in dairy herds.

Escherichia coli O157 and non-O157 Shiga Toxin–Producing Escherichia coli in Fecal Samples of Finished Pigs at Slaughter in Switzerland

2006 ◽  
Vol 69 (2) ◽  
pp. 260-266 ◽  
Author(s):  
M. KAUFMANN ◽  
C. ZWEIFEL ◽  
M. BLANCO ◽  
J. E. BLANCO ◽  
J. BLANCO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
Strongly Correlated ◽  
Fecal Samples ◽  
E Coli ◽  
High Prevalence ◽  
Strain Testing ◽  
Typical Epec ◽  
Uremic Syndrome

Fecal samples from 630 slaughtered finisher pigs were examined by PCR to assess the shedding of Escherichia coli O157 (rfbE) and Shiga toxin–producing E. coli (STEC, stx). The proportion of positive samples was 7.5% for rfbE and 22% for stx. By colony hybridization, 31 E. coli O157 and 45 STEC strains were isolated, and these strains were further characterized by phenotypic and genotypic traits. Among E. coli O157 strains, 30 were sorbitol positive, 30 had an H type other than H7, and none harbored stx genes. Intimin (eae), enterohemolysin (ehxA), EAST1 (astA), and porcine A/E–associated protein (paa) were present in 10, 3, 26, and 6% of strains. Among them, one eae-γ1–positive O157:H7 strain testing positive for ehxA and astA and two eae-α1–positive O157:H45 strains were classified as enteropathogenic E. coli (EPEC). The O157:H45 EPEC harbored the EAF plasmid and the bfpA gene, factors characteristic for typical EPEC. The isolated STEC strains (43 sorbitol positive) belonged to 11 O:H serotypes, including three previously reported in human STEC causing hemolytic uremic syndrome (O9:H−, O26:H−, and O103:H2). All but one strain harbored stx2e. The eae and ehxA genes, which are strongly correlated with human disease, were present in only one O103:H2 strain positive for stx1 and paa, whereas the astA gene was found more frequently (14 strains). High prevalence of STEC was found among finisher pigs, but according to the virulence factors the majority of these strains seem to be of low virulence.

scholarly journals Prevalence of Escherichia coli O157:H7 in range beef calves at weaning

1999 ◽  
Vol 123 (2) ◽  
pp. 291-298 ◽  
Author(s):  
W. W. LAEGREID ◽  
R. O. ELDER ◽  
J. E. KEEN
Keyword(s):  
Escherichia Coli ◽  
Serum Antibody ◽  
Escherichia Coli O157 ◽  
Beef Calves ◽  
E Coli ◽  
Faecal Culture ◽  
Faecal Samples ◽  
High Prevalence ◽  
Antibody Titres ◽  
Serologic Evidence

This study was designed to determine the prevalence of Escherichia coli O157:H7 infection of beef calves at weaning, prior to arrival at the feedlot or mixing with cattle from other sources. Fifteen range cow-calf herds, which weaned calves in October and November, were sampled in Kansas, Missouri, Montana, Nebraska and South Dakota. Faecal culture for E. coli O157:H7 was performed and anti-O157 serum antibody titres were determined by blocking ELISA. Thirteen of the 15 herds (87%) were found to have at least one positive isolation of E. coli O157:H7 in faecal samples. Within positive herds, prevalence ranged from 1·7–20·0%, with an average of 7·4±6·2% s.d. of individual animals shedding E. coli O157:H7 in faeces. All herds had high prevalence of anti-O157 antibodies, ranging 63–100% of individuals within herds seropositive. This study indicates that E. coli O157:H7 infection before weaning, prior to entry into feedlots, is widespread. Furthermore, serologic evidence suggests that most calves (83%) and all herds (100%) have been exposed to E. coli O157.

Salmonella and Escherichia coli O157:H7 Prevalence in Cattle and on Carcasses in a Vertically Integrated Feedlot and Harvest Plant in Mexico

2013 ◽  
Vol 76 (5) ◽  
pp. 786-795 ◽  
Author(s):  
C. NARVAEZ-BRAVO ◽  
M. F. MILLER ◽  
T. JACKSON ◽  
S. JACKSON ◽  
A. RODAS-GONZALEZ ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Significant Risk ◽  
Significant Risk Factor ◽  
Immunomagnetic Separation ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
High Prevalence ◽  
Good Manufacturing Practices ◽  
The Impact ◽  
Composite Samples

To determine the prevalence of Salmonella and Escherichia coli O157:H7 in cattle feedlots and the impact of subsequent contamination on carcasses in a Mexican Federal Inspection Type Standards harvest facility, 250 animals were tagged and sampled in each step of the slaughter process. Samples were taken from hides and fecal grabs, and composite samples were taken from three anatomical carcass sites (hindshank, foreshank, and inside round) during the slaughter process, at preevisceration (PE), prior to entering the hot box (PHB), and after 24 h of dry chilling (DC). Additionally, 250 fecal samples were collected from the feedlot (FL), holding pens (HP), and intestinal feces (IF), and water samples were taken from the HP area. E. coli O157:H7 and Salmonella detection were carried out with the BAX System, immunomagnetic separation, and conventional methods. Overall Salmonella prevalence was 52.5%. The highest prevalence (92.4%) was found on hides, followed by feces from the HP (91.0%), FL (55.56%), PE (49.0%), IF (46.8%), and PHB (24.8%), for all sampling periods combined. The lowest prevalence of 6.0% was found after DC. The overall prevalence of E. coli O157:H7 was as follows: 11.7% for hides, 5.2% for IF, 2.7% for FL, 2.0% for HP, 0.8% for PE, 0.4% for PHB, and 0.4% for the cooler. High prevalence of Salmonella in IF and on hides present a significant risk factor for contamination by Salmonella at the different processing steps. These results serve as a warning as to the risks of contamination in meats for these pathogens and the importance of following good manufacturing practices during beef production processes.

scholarly journals Molecular Characterization of Escherichia coli O157:H7 and non-O157 Shiga Toxin Producing E. coli from Retail Meat and Humans

2017 ◽  
Vol 45 (3) ◽  
pp. 250-261 ◽  
Author(s):  
Heba Ahmed ◽  
Ewan MacLeod ◽  
Rasha El Bayomi ◽  
Rasha Mohsen ◽  
Arwa Nassar
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Shiga Toxin ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Retail Meat

Bedeutung von Escherichia coli O157 beim Schlachtschaf in der Schweiz

2006 ◽  
Vol 148 (6) ◽  
pp. 289-295 ◽  
Author(s):  
C. Zweifel ◽  
M. Kaufmann ◽  
J. Blanco ◽  
R. Stephan
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157

Molecular profiling and antimicrobial susceptibility of Escherichia coli O157:H7 isolated in Bulgaria

2020 ◽  
Vol 23 (3) ◽  
pp. 310-318
Author(s):  
K. Koev ◽  
T. Stoyanchev ◽  
G. Zhelev ◽  
P. Marutsov ◽  
K. Gospodinova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Acid Sensitivity ◽  
Lower Extent ◽  
Phenotype Analysis ◽  
Anal Swab ◽  
Amoxicillin Clavulanic Acid ◽  
Primary Identification ◽  
Stx1 Gene ◽  
Cattle Farms

The purpose of this study was to detect the presence of shiga-toxin producing Escherichia coli (STEC) in faeces of healthy dairy cattle and to determine the sensitivity of isolates to several anti­microbial drugs. A total of 1,104 anal swab samples originating from 28 cattle farms were examined. After the primary identification, 30 strains were found to belong to serogroup О157. By means of conventional multiplex PCR, isolates were screened for presence of resistance genes stx1, stx2 and eaeА. Twenty-nine strains possesses amplicons with a size corresponding to genes stx2 and eaeA, one had amplicons also for the stx1 gene and one lacked amplicons of all three genes. Twenty-eight strains demonstrated amplicons equivalent to gene H7. The results from phenotype analysis of resistance showed preserved sensitivity to ceftriaxone, ceftazidime, cefotaxime, cephalothin, streptomycin, gentamicin, tetracycline, enrofloxacin and combinations sulfamethoxazole/trimethoprim and amoxicillin/clavulanic acid. Sensitivity to ampicillin was relatively preserved, although at a lower extent.

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