Genetically highly divergent RNA virus with astrovirus-like (5′-end) and hepevirus-like (3′-end) genome organization in carnivorous birds, European roller (Coracias garrulus)

We present the genome organization and molecular characterization of the three Formica exsecta viruses, along with ORF predictions, and functional annotation of genes. The Formica exsecta virus-4 (FeV4; GenBank ID: MF287670) is a newly discovered negative-sense single-stranded RNA virus representing the first identified member of order Mononegavirales in ants, whereas the Formica exsecta virus-1 (FeV1; GenBank ID: KF500001), and the Formica exsecta virus-2 (FeV2; GenBank ID: KF500002) are positive single-stranded RNA viruses initially identified (but not characterized) in our earlier study. The new virus FeV4 was found by re-analyzing data from a study published earlier. The Formica exsecta virus-4 genome is 9,866 bp in size, with an overall G + C content of 44.92%, and containing five predicted open reading frames (ORFs). Our bioinformatics analysis indicates that gaps are absent and the ORFs are complete, which based on our comparative genomics analysis suggests that the genomes are complete. Following the characterization, we validate virus infection for FeV1, FeV2 and FeV4 for the first time in field-collected worker ants. Some colonies were infected by multiple viruses, and the viruses were observed to infect all castes, and multiple life stages of workers and queens. Finally, highly similar viruses were expressed in adult workers and queens of six other Formica species: F. fusca, F. pressilabris, F. pratensis, F. aquilonia, F. truncorum and F. cinerea. This research indicates that viruses can be shared between ant species, but further studies on viral transmission are needed to understand viral infection pathways.

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Genetic characterization of a novel picornavirus distantly related to the marine mammal-infecting aquamaviruses in a long-distance migrant bird species, European roller (Coracias garrulus)

Journal of General Virology ◽

10.1099/vir.0.054676-0 ◽

2013 ◽

Vol 94 (9) ◽

pp. 2029-2035 ◽

Cited By ~ 19

Author(s):

Ákos Boros ◽

Tamás Kiss ◽

Orsolya Kiss ◽

Péter Pankovics ◽

Beatrix Kapusinszky ◽

...

Keyword(s):

Marine Mammal ◽

Genetic Characterization ◽

Phylogenetic Analyses ◽

Bird Species ◽

Long Distance ◽

Coracias Garrulus ◽

Faecal Samples ◽

European Roller ◽

Migrant Bird

Despite the continuously growing number of known avian picornaviruses (family Picornaviridae), knowledge of their genetic diversity in wild birds, especially in long-distance migrant species is very limited. In this study, we report the presence of a novel picornavirus identified from one of 18 analysed faecal samples of an Afro-Palearctic migrant bird, the European roller (Coracias garrulus L., 1758), which is distantly related to the marine-mammal-infecting seal aquamavirus A1 (genus Aquamavirus). The phylogenetic analyses and the low sequence identity (P1 26.3 %, P2 25.8 % and P3 28.4 %) suggest that this picornavirus could be the founding member of a novel picornavirus genus that we have provisionally named ‘Kunsagivirus’, with ‘Greplavirus A’ (strain roller/SZAL6-KuV/2011/HUN, GenBank accession no. KC935379) as the candidate type species.

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LONG-TERM MONITORING OF THE EUROPEAN ROLLER (CORACIAS GARRULUS) IN UKRAINE: IS CLIMATE BEHIND THE CHANGES?

10.32942/osf.io/6t7uq ◽

2021 ◽

Author(s):

Tatiana Shupova ◽

Volodymyr Tytar

Keyword(s):

Spatially Explicit ◽

Climate Data ◽

Time Intervals ◽

Coracias Garrulus ◽

The Past ◽

Historical Times ◽

Long Term Monitoring ◽

European Roller ◽

Term Monitoring

Since the 1980s there has been a long-term decline in numbers and contraction of range in Europe, including Ukraine. Our specific goals were to reconstruct the climatically suitable range of the species in Ukraine before the 1980s, gain better knowledge on its requirements, compare the past and current suitable areas, infer the regional and environmental variables that best explain its occurrence, and quantify the overall range change in the country. For these purposes we created a database consisting of 347 records of the roller made ever in Ukraine. We employed a species distribution modeling (SDM) approach to hindcast changes in the suitable range of the roller during historical times across Ukraine and to derive spatially explicit predictions of climatic suitability for the species under current climate. SDMs were created for three time intervals (before 1980, 1985-2009, 2010-2021) using corresponding climate data extracted from the TerraClim database. SDMs show a decline of suitable for rollers areas in the country from 85 to 46%. Several factors, including land cover and use, human population density and climate, that could have contributed to the decline of the species in Ukraine were considered. We suggest climate change and its speed (velocity) have been responsible for shaping the contemporary home range of the European roller.

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Reproductive behavior of the european roller (Coracias garrulus Linnaeus, 1758)

E3S Web of Conferences ◽

10.1051/e3sconf/202126501007 ◽

2021 ◽

Vol 265 ◽

pp. 01007

Author(s):

Liubov Malovichko ◽

Nadezhda Poddubnaya ◽

Karina Akimova ◽

Liubov Eltsova

Keyword(s):

Behavioral Ecology ◽

Coracias Garrulus ◽

Parental Roles ◽

European Part Of Russia ◽

Wintering Areas ◽

Preliminary Study ◽

European Roller ◽

And Behavior ◽

High Coordination

In the European part of Russia, there is a decrease in the area and number of the European Roller. We performed a preliminary study of the role of behavioral ecology in the survival of the species. The material was collected in the Stavropol krai in 2004, 2006-2019. Females and males were distinguished by color intensity (females' plumage is paler) and behavior. Birds exhibit complex biocommunication with features of a foraging economy, distribution of parental roles, and high coordination of partner actions. Additional research is needed to elucidate the behavior of birds whose partners died in the flyover or wintering areas.

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Complete Genome Sequence of Diaphorina citri-associated C virus , a Novel Putative RNA Virus of the Asian Citrus Psyllid, Diaphorina citri

Genome Announcements ◽

10.1128/genomea.00639-16 ◽

2016 ◽

Vol 4 (4) ◽

Cited By ~ 4

Author(s):

Shahideh Nouri ◽

Nidà Salem ◽

Bryce W. Falk

Keyword(s):

Nucleotide Sequence ◽

Complete Genome Sequence ◽

Genome Organization ◽

Complete Nucleotide Sequence ◽

Rna Virus ◽

Asian Citrus Psyllid ◽

Diaphorina Citri ◽

Rt Pcr ◽

Reverse Transcription Pcr ◽

Positive Sense

We present here the complete nucleotide sequence and genome organization of a novel putative RNA virus identified in field populations of the Asian citrus psyllid, Diaphorina citri , through sequencing of the transcriptome followed by reverse transcription-PCR (RT-PCR). We tentatively named this virus Diaphorina citri-associated C virus (DcACV). DcACV is an unclassified positive-sense RNA virus.

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Coracias garrulus Linnaeus. European Roller. Rollier d’Europe.

The Birds of Africa ◽

10.5040/9781472927002.0194 ◽

2016 ◽

Keyword(s):

Coracias Garrulus ◽

European Roller

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A novel RNA mycovirus in a hypovirulent isolate of the plant pathogen Diaporthe ambigua

Journal of General Virology ◽

10.1099/0022-1317-81-12-3107 ◽

2000 ◽

Vol 81 (12) ◽

pp. 3107-3114 ◽

Cited By ~ 60

Author(s):

O. Preisig ◽

N. Moleleki ◽

W. A. Smit ◽

B. D. Wingfield ◽

M. J. Wingfield

Keyword(s):

Genome Organization ◽

Rna Virus ◽

Plant Viruses ◽

Translation Product ◽

Terminal Part ◽

Virus Family ◽

Positive Strand ◽

Positive Strand Rna Virus ◽

Positive Strand Rna ◽

Diaporthe Ambigua

Hypovirulent isolates of the fruit tree fungal pathogen Diaporthe ambigua have previously been shown to harbour a double-stranded (ds)RNA genetic element of about 4 kb. In this study, we established the complete cDNA sequence of this dsRNA, which represents a replicative form of a positive-strand RNA virus that we have named D. ambigua RNA virus (DaRV). The nucleotide sequence of the genome is 4113 bp and has a GC content of 53%. Two large ORFs are present in the same reading frame. They are most probably translated by readthrough of a UAG stop codon in the central part of the genome. The longest possible translation product (p125) has a predicted molecular mass of about 125 kDa. A significant homology can be found to the non-structural proteins of carmoviruses of the positive-strand RNA virus family Tombusviridae. These proteins also include the conserved RNA-dependent RNA polymerase (RDRP) domain. In contrast to the genome organization of these plant viruses, no ORF is present at the 3′ end of the DaRV genome that encodes a coat protein. Therefore, it is proposed that DaRV is not encapsidated but that it occurs as RNA–RDRP complexes and/or that it might be associated with cell membranes. Interestingly, six putative transmembrane helices are predicted in the N-terminal part of p56 (translation product of the first ORF, N-terminal part of p125), which might direct and anchor the viral complex to membranes. DaRV is a mycovirus with a unique genome organization and has a distant relationship to the plant virus family Tombusviridae.

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