scholarly journals Genetic characterization of a novel picornavirus distantly related to the marine mammal-infecting aquamaviruses in a long-distance migrant bird species, European roller (Coracias garrulus)

2013 ◽  
Vol 94 (9) ◽  
pp. 2029-2035 ◽  
Author(s):  
Ákos Boros ◽  
Tamás Kiss ◽  
Orsolya Kiss ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
...  
Keyword(s):  
Marine Mammal ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Bird Species ◽  
Long Distance ◽  
Coracias Garrulus ◽  
Faecal Samples ◽  
European Roller ◽  
Migrant Bird

Despite the continuously growing number of known avian picornaviruses (family Picornaviridae), knowledge of their genetic diversity in wild birds, especially in long-distance migrant species is very limited. In this study, we report the presence of a novel picornavirus identified from one of 18 analysed faecal samples of an Afro-Palearctic migrant bird, the European roller (Coracias garrulus L., 1758), which is distantly related to the marine-mammal-infecting seal aquamavirus A1 (genus Aquamavirus). The phylogenetic analyses and the low sequence identity (P1 26.3 %, P2 25.8 % and P3 28.4 %) suggest that this picornavirus could be the founding member of a novel picornavirus genus that we have provisionally named ‘Kunsagivirus’, with ‘Greplavirus A’ (strain roller/SZAL6-KuV/2011/HUN, GenBank accession no. KC935379) as the candidate type species.

scholarly journals Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)

2012 ◽  
Vol 93 (10) ◽  
pp. 2171-2182 ◽  
Author(s):  
Ákos Boros ◽  
Csaba Nemes ◽  
Péter Pankovics ◽  
Beatrix Kapusinszky ◽  
Eric Delwart ◽  
...  
Keyword(s):  
Amino Acid ◽  
Complete Genome ◽  
Bird Species ◽  
Entry Site ◽  
Pathogenic Potential ◽  
Genome Characterization ◽  
Genome Region ◽  
Faecal Samples ◽  
The Usa

Members of the family Picornaviridae are important pathogens of humans and animals, although compared with the thousands of known bird species (>10 000), only a few (n = 11) picornaviruses have been identified from avian sources. This study reports the metagenomic detection and complete genome characterization of a novel turkey picornavirus from faecal samples collected from eight turkey farms in Hungary. Using RT-PCR, both healthy (two of three) and affected (seven of eight) commercial turkeys with enteric and/or stunting syndrome were shown to be shedding viruses in seven (88 %) of the eight farms. The viral genome sequence (turkey/M176/2011/HUN; GenBank accession no. JQ691613) shows a high degree of amino acid sequence identity (96 %) to the partial P3 genome region of a picornavirus reported recently in turkey and chickens from the USA and probably belongs to the same species. In the P1 and P2 regions, turkey/M176/2011/HUN is related most closely to, but distinct from, the kobuviruses and turdivirus 1. Complete genome analysis revealed the presence of characteristic picornaviral amino acid motifs, a potential type II-like 5′ UTR internal ribosome entry site (first identified among avian-origin picornaviruses) and a conserved, 48 nt long ‘barbell-like’ structure found at the 3′ UTR of turkey/M176/2011/HUN and members of the picornavirus genera Avihepatovirus and Kobuvirus. The general presence of turkey picornavirus – a novel picornavirus species – in faecal samples from healthy and affected turkeys in Hungary and in the USA suggests the worldwide occurrence and endemic circulation of this virus in turkey farms. Further studies are needed to investigate the aetiological role and pathogenic potential of this picornavirus in food animals.

scholarly journals Genetic Characterization of Vibrio vulnificus Strains from Tilapia Aquaculture in Bangladesh

2010 ◽  
Vol 76 (14) ◽  
pp. 4890-4895 ◽  
Author(s):  
Zahid H. Mahmud ◽  
Anita C. Wright ◽  
Shankar C. Mandal ◽  
Jianli Dai ◽  
Melissa K. Jones ◽  
...  
Keyword(s):  
Vibrio Vulnificus ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Wound Infections ◽  
Freshwater Aquaculture

ABSTRACT Outbreaks of Vibrio vulnificus wound infections in Israel were previously attributed to tilapia aquaculture. In this study, V. vulnificus was frequently isolated from coastal but not freshwater aquaculture in Bangladesh. Phylogenetic analyses showed that strains from Bangladesh differed remarkably from isolates commonly recovered elsewhere from fish or oysters and were more closely related to strains of clinical origin.

Characterization of 28 microsatellite loci in the European roller Coracias garrulus (Coracidae, AVES)

2014 ◽  
Vol 60 (5) ◽  
pp. 843-850 ◽  
Author(s):  
David Martín-Gálvez ◽  
Mercedes Molina-Morales ◽  
Deborah A. Dawson ◽  
Deseada Parejo ◽  
Juan Gabriel Martínez ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Coracias Garrulus ◽  
European Roller

Complete genome characterization of mosavirus (family Picornaviridae) identified in droppings of a European roller (Coracias garrulus) in Hungary

2014 ◽  
Vol 159 (10) ◽  
pp. 2723-2729 ◽  
Author(s):  
Gábor Reuter ◽  
Ákos Boros ◽  
Tamás Kiss ◽  
Eric Delwart ◽  
Péter Pankovics
Keyword(s):  
Complete Genome ◽  
Coracias Garrulus ◽  
Genome Characterization ◽  
European Roller

scholarly journals Identification and Genetic Characterization of a Novel Respirovirus in Alpine Chamois (Rupicapra rupicapra rupicapra)

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 704
Author(s):  
Camilla Luzzago ◽  
Erika Ebranati ◽  
Antonio Lavazza ◽  
Martina Besozzi ◽  
Gianguglielmo Zehender ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Amino Acid Sequences ◽  
Species Demarcation Criterion ◽  
Demarcation Criterion ◽  
Protein Amino Acid ◽  
Rupicapra Rupicapra ◽  
Separate Branch ◽  
Alpine Chamois

The Respirovirus genus, family Paramamixoviridae, includes respiratory viral pathogens. Here we report the identification and genetic characterization of a respirovirus in an Alpine chamois showing interstitial pneumonia associated with catarrhal bronchopneumonia. The full-genome characterization of this respirovirus, named ChamoisRV/IT2014, revealed low similarities to caprine respirovirus (77.1%), bovine respirovirus (74.5%) and human respirovirus (72.0%). The phylogenetic analyses based on the full-length genome sequence of the novel isolate and reference respirovirus strains showed that ChamoisRV/IT2014 clustered with caprine respirovirus but formed a separate branch. The phylogenetic tree topology of complete large protein amino acid sequences, representing the current species demarcation criterion for Respirovirus genus, showed a 0.05 branch length of ChamoisRV/IT2014 sequence between the nearest node and the tip of the branch, suggesting that this virus belongs to a novel species. This new isolate in a new host species raises several questions to be addressed on the epidemiological role of chamois and the risks of cross-transmission between wild ruminants and livestock.

scholarly journals Molecular and Biological Characterization of a Cryptosporidium molnari-Like Isolate from a Guppy (Poecilia reticulata)

2004 ◽  
Vol 70 (6) ◽  
pp. 3761-3765 ◽  
Author(s):  
Una Ryan ◽  
Amanda O'Hara ◽  
Lihua Xiao
Keyword(s):  
Poecilia Reticulata ◽  
Genetic Characterization ◽  
Phylogenetic Analyses ◽  
Biological Characterization

ABSTRACT Histological, morphological, genetic, and phylogenetic analyses of a Cryptosporidium molnari-like isolate from a guppy (Poecilia reticulata) identified stages consistent with those of C. molnari and revealed that C. molnari is genetically very distinct from all other species of Cryptosporidium. This study represents the first genetic characterization of C. molnari.

Phenological differences among selected residents and long-distance migrant bird species in central Europe

2013 ◽  
Vol 58 (5) ◽  
pp. 809-817 ◽  
Author(s):  
Lenka Bartošová ◽  
Miroslav Trnka ◽  
Zdeněk Bauer ◽  
Martin Možný ◽  
Petr Štěpánek ◽  
...  
Keyword(s):  
Central Europe ◽  
Bird Species ◽  
Long Distance ◽  
Migrant Bird

scholarly journals Genetic characterization of nodular worm infections in Asian Apes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Erhan Yalcindag ◽  
Peter Stuart ◽  
Hideo Hasegawa ◽  
Adrian Streit ◽  
Jana Doležalová ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Parasitic Nematodes ◽  
Infection Prevalence ◽  
Wild Animals ◽  
Significant Genetic Differentiation ◽  
Population Genetic Analysis ◽  
Faecal Samples ◽  
Free Ranging ◽  
First Time

AbstractParasitic nematodes of Oesophagostomum spp., commonly known, as 'nodular worms' are emerging as the most widely distributed and prevalent zoonotic nematodes. Oesophagostomum infections are well documented in African non-human primates; however, the taxonomy, distribution and transmission of Oesophagostomum in Asian non-human primates are not adequately studied. To better understand which Oesophagostomum species infect Asian non-human primates and determine their phylogeny we analysed 55 faecal samples from 50 orangutan and 5 gibbon individuals from Borneo and Sumatra. Both microscopy and molecular results revealed that semi-wild animals had higher Oesophagostomum infection prevalence than free ranging animals. Based on sequence genotyping analysis targeting the Internal transcribed spacer 2 of rDNA, we report for the first time the presence of O. aculeatum in Sumatran apes. Population genetic analysis shows that there is significant genetic differentiation between Bornean and Sumatran O. aculeatum populations. Our results clearly reveal that O. aculeatum in free-ranging animals have a higher genetic variation than those in semi-wild animals, demonstrating that O. aculeatum is circulating naturally in wildlife and zoonotic transmission is possible. Further studies should be conducted to better understand the epidemiology and dynamics of Oesophagostomum transmission between humans, non-human primates and other wild species and livestock in Southeast Asia.

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