scholarly journals Identification and characterization of bacterial isolates from the Mir space station

2005 ◽  
Vol 160 (2) ◽  
pp. 111-117 ◽  
Author(s):  
Bo Song ◽  
Laura G. Leff
Keyword(s):  
Space Station ◽  
Bacterial Isolates ◽  
Identification And Characterization

scholarly journals PEMANFAATAN BAKTERI ANTAGONIS LUMPUR SIDOARJO UNTUK MENEKAN Sclerotium rolfsiiSACC. PENYEBAB PENYAKIT REBAH SEMAI PADA TANAMAN KEDELAI

2019 ◽  
Vol 13 (1) ◽  
pp. 11-20
Author(s):  
Intan Fuji Arriani
Keyword(s):  
Pathogenic Fungi ◽  
Antagonistic Bacteria ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Soybean Plants ◽  
Genus Corynebacterium ◽  
Identification And Characterization ◽  
Corynebacterium Sp ◽  
Selection Of

ABSTRACT Soybean plants is a strategic commodity in Indonesia, because soybean is an important crop food after rice and maize. One of the important diseases that attack soybean plants is damping off caused by pathogenic fungi S. rolfsii. One effort to suppress attack from pathogenic fungi it S. rolfsii attack is by using biological agents. Trial of antagonistic bacteria with fungi S. rolfsii  stepin vitro is test the artificial media (media NA). The result of the selection of the lumpur Sidoarjo bacterial isolates obtained 15 and 5 bacteria have antagonistic properties against pathogenic fungi S. rolfsii is  isolates LUSI 93, LUSI 43, LUSI 16, LUSI 6, and LUSI 54. The identification and characterization of antagonistic bacteria lumpur Sidoarjo is LUSI 93 belongs to the genus Vibrio sp. And for LUSI 54, 16 LUSI, LUSI 43, and LUSI 6 has been identified by previous research in a row belongs to the genus Corynebacterium sp, Vibrio sp, Erwinia sp and Corynebacterium sp.

scholarly journals Identification and Characterization of Bacteria in a Selenium-Contaminated Hypersaline Evaporation Pond

2001 ◽  
Vol 67 (9) ◽  
pp. 3785-3794 ◽  
Author(s):  
M. P. de Souza ◽  
A. Amini ◽  
M. A. Dojka ◽  
I. J. Pickering ◽  
S. C. Dawson ◽  
...  
Keyword(s):  
Health Hazard ◽  
Drainage Water ◽  
San Joaquin Valley ◽  
Agricultural Drainage ◽  
Bacterial Isolates ◽  
Evaporation Ponds ◽  
Uncultured Bacteria ◽  
X Ray Absorption ◽  
Identification And Characterization

ABSTRACT Solar evaporation ponds are commonly used to reduce the volume of seleniferous agricultural drainage water in the San Joaquin Valley, Calif. These hypersaline ponds pose an environmental health hazard because they are heavily contaminated with selenium (Se), mainly in the form of selenate. Se in the ponds may be removed by microbial Se volatilization, a bioremediation process whereby toxic, bioavailable selenate is converted to relatively nontoxic dimethylselenide gas. In order to identify microbes that may be used for Se bioremediation, a 16S ribosomal DNA phylogenetic analysis of an aerobic hypersaline pond in the San Joaquin Valley showed that a previously unaffiliated group of uncultured bacteria (belonging to the orderCytophagales) was dominant, followed by a group of cultured γ-Proteobacteria which was closely related to Halomonas species. Se K-edge X-ray absorption spectroscopy of selenate-treated bacterial isolates showed that they accumulated a mixture of predominantly selenate and a selenomethionine-like species, consistent with the idea that selenate was assimilated via the S assimilation pathway. One of these bacterial isolates (Halomonas-like strain MPD-51) was the best candidate for the bioremediation of hypersaline evaporation ponds contaminated with high Se concentrations because it tolerated 2 M selenate and 32.5% NaCl, grew rapidly in media containing selenate, and accumulated and volatilized Se at high rates (1.65 μg of Se g of protein−1 h−1), compared to other cultured bacterial isolates.

scholarly journals Identification and characterization of thermophilic amylase producing bacterial isolates from the brick kiln soil

2021 ◽  
Vol 28 (1) ◽  
pp. 970-979
Author(s):  
Irfan Ullah ◽  
Mohammad Sayyar Khan ◽  
Shahin Shah Khan ◽  
Waqar Ahmad ◽  
Lingjie Zheng ◽  
...  
Keyword(s):  
Bacterial Isolates ◽  
Brick Kiln ◽  
Identification And Characterization

scholarly journals Limited aromatic pathway genes diversity amongst aromatic compound degrading soil bacterial isolates

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 703-716 ◽  
Author(s):  
Gordana Minovska ◽  
Tanja Narancic ◽  
Mina Mandic ◽  
Lidija Senerovic ◽  
Branka Vasiljevic ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Bacterial Isolates ◽  
Degenerate Primers ◽  
Novel Genes ◽  
Biodegradation Pathway ◽  
Wide Range ◽  
Aromatic Pathway ◽  
Identification And Characterization ◽  
Selection Of

Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterized corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.

scholarly journals Identity and Antibiogram of Bacterial Isolates from Owerri Modern Abattoir, Imo State, Nigeria

2021 ◽  
pp. 43-54
Author(s):  
Joy Nkeiruka Dike-Ndudim ◽  
Roseline Nwanneka Ugwuegbu ◽  
Henry Chidozie Amah ◽  
Chizaram Winners Ndubueze ◽  
Emeka Simon Anikwo
Keyword(s):  
Demographic Data ◽  
Cross Sectional Study ◽  
Bacterial Isolates ◽  
Cross Sectional ◽  
Imo State ◽  
Colony Counting ◽  
Identification And Characterization ◽  
Washing Water ◽  
Government Authority

Aim: The identity and antibiogram of bacterial isolates from Owerri modern abattoir in Imo State, Nigeria, was investigated with the aim of determining the bacterial profile of the abattoir and their susceptibility pattern to commonly used antibiotics. Study Design: Cross sectional study Place and Duration of Study: This study was carried out in Owerri Modern Abattoir located within Owerri metropolis from June to November, 2020. Methodology: Questionnaires were used to obtain participants’ consent, demographic data and sanitary practices in the abattoir.  Samples were taken and bacteriological analysis of the samples done using pour plate method. Disc diffusion antibiotic susceptibility testing and minimum inhibitory concentration were performed after colony counting, identification and characterization of the isolates using standard microbiological and biochemical techniques. Results: Mean viable bacterial counts were generally high with highest counts from contaminated soil (6.13x106CFU/ml) and least from workers hands (1.17x106CFU/ml). Escherichia coli had the highest prevalence (18.0%), with the highest counts from soil (3.10%). Vibrio cholerae recorded the least prevalence (0.62%), and was isolated only from washing water. High resistance to antibiotics was observed. Conclusion: Government authority and the general public are advised to ensure adequate environmental sanitation and proper cooking of meat before consumption in order to mitigate the incidence of infection and antibiotic resistance.

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