scholarly journals PEMANFAATAN BAKTERI ANTAGONIS LUMPUR SIDOARJO UNTUK MENEKAN Sclerotium rolfsiiSACC. PENYEBAB PENYAKIT REBAH SEMAI PADA TANAMAN KEDELAI

2019 ◽  
Vol 13 (1) ◽  
pp. 11-20
Author(s):  
Intan Fuji Arriani
Keyword(s):  
Pathogenic Fungi ◽  
Antagonistic Bacteria ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Soybean Plants ◽  
Genus Corynebacterium ◽  
Identification And Characterization ◽  
Corynebacterium Sp ◽  
Selection Of

ABSTRACT Soybean plants is a strategic commodity in Indonesia, because soybean is an important crop food after rice and maize. One of the important diseases that attack soybean plants is damping off caused by pathogenic fungi S. rolfsii. One effort to suppress attack from pathogenic fungi it S. rolfsii attack is by using biological agents. Trial of antagonistic bacteria with fungi S. rolfsii  stepin vitro is test the artificial media (media NA). The result of the selection of the lumpur Sidoarjo bacterial isolates obtained 15 and 5 bacteria have antagonistic properties against pathogenic fungi S. rolfsii is  isolates LUSI 93, LUSI 43, LUSI 16, LUSI 6, and LUSI 54. The identification and characterization of antagonistic bacteria lumpur Sidoarjo is LUSI 93 belongs to the genus Vibrio sp. And for LUSI 54, 16 LUSI, LUSI 43, and LUSI 6 has been identified by previous research in a row belongs to the genus Corynebacterium sp, Vibrio sp, Erwinia sp and Corynebacterium sp.

scholarly journals Limited aromatic pathway genes diversity amongst aromatic compound degrading soil bacterial isolates

Genetika ◽  
2013 ◽  
Vol 45 (3) ◽  
pp. 703-716 ◽  
Author(s):  
Gordana Minovska ◽  
Tanja Narancic ◽  
Mina Mandic ◽  
Lidija Senerovic ◽  
Branka Vasiljevic ◽  
...  
Keyword(s):  
Environmental Samples ◽  
Bacterial Isolates ◽  
Degenerate Primers ◽  
Novel Genes ◽  
Biodegradation Pathway ◽  
Wide Range ◽  
Aromatic Pathway ◽  
Identification And Characterization ◽  
Selection Of

Identification and characterization of novel genes belonging to microbial aromatic biodegradation pathway is of great importance as they have been proven versatile biocatalysts. In this study, the selection of 19 environmental bacterial isolates capable to degrade a wide range of aromatic compounds has been screened for the presence of five genes from the lower and the upper aromatic biodegradation pathway using PCR methodology. In the case of 4-oxalocrotonate tautomerase and toluene dioxygenases, although present in the most of environmental isolates, very limited diversity of the genes has been encountered. Highly conserved sequences of these genes in environmental samples revealed high homology with gene sequences of the characterized corresponding genes from Pseudomonas putida species. The screen using degenerate primers based on known catechol-and naphthalene dioxygenases sequences resulted in a limited number of amplified fragments. Only two catechol 2,3-dioxygenase from two Bacillus isolates were amplified and showed no significant similarities with dioxygenases from characterized organisms, but 80-90% identities with partial catechol 2,3-dioxygenase sequences from uncultured organisms. Potentially three novel catechol 1,2-dioxygenases were identified from Bacillus sp. TN102, Gordonia sp. TN103 and Rhodococcus sp. TN112. Highly homologous tautomerase and toluene dioxygenases amongst environmental samples isolated from the contaminated environment suggested horizontal gene transfer while limited success in PCR detection of the other three genes indicates that these isolates may still be a source of novel genes.

scholarly journals Identification and Characterization of Bacteria in a Selenium-Contaminated Hypersaline Evaporation Pond

2001 ◽  
Vol 67 (9) ◽  
pp. 3785-3794 ◽  
Author(s):  
M. P. de Souza ◽  
A. Amini ◽  
M. A. Dojka ◽  
I. J. Pickering ◽  
S. C. Dawson ◽  
...  
Keyword(s):  
Health Hazard ◽  
Drainage Water ◽  
San Joaquin Valley ◽  
Agricultural Drainage ◽  
Bacterial Isolates ◽  
Evaporation Ponds ◽  
Uncultured Bacteria ◽  
X Ray Absorption ◽  
Identification And Characterization

ABSTRACT Solar evaporation ponds are commonly used to reduce the volume of seleniferous agricultural drainage water in the San Joaquin Valley, Calif. These hypersaline ponds pose an environmental health hazard because they are heavily contaminated with selenium (Se), mainly in the form of selenate. Se in the ponds may be removed by microbial Se volatilization, a bioremediation process whereby toxic, bioavailable selenate is converted to relatively nontoxic dimethylselenide gas. In order to identify microbes that may be used for Se bioremediation, a 16S ribosomal DNA phylogenetic analysis of an aerobic hypersaline pond in the San Joaquin Valley showed that a previously unaffiliated group of uncultured bacteria (belonging to the orderCytophagales) was dominant, followed by a group of cultured γ-Proteobacteria which was closely related to Halomonas species. Se K-edge X-ray absorption spectroscopy of selenate-treated bacterial isolates showed that they accumulated a mixture of predominantly selenate and a selenomethionine-like species, consistent with the idea that selenate was assimilated via the S assimilation pathway. One of these bacterial isolates (Halomonas-like strain MPD-51) was the best candidate for the bioremediation of hypersaline evaporation ponds contaminated with high Se concentrations because it tolerated 2 M selenate and 32.5% NaCl, grew rapidly in media containing selenate, and accumulated and volatilized Se at high rates (1.65 μg of Se g of protein−1 h−1), compared to other cultured bacterial isolates.

scholarly journals Identification and characterization of thermophilic amylase producing bacterial isolates from the brick kiln soil

2021 ◽  
Vol 28 (1) ◽  
pp. 970-979
Author(s):  
Irfan Ullah ◽  
Mohammad Sayyar Khan ◽  
Shahin Shah Khan ◽  
Waqar Ahmad ◽  
Lingjie Zheng ◽  
...  
Keyword(s):  
Bacterial Isolates ◽  
Brick Kiln ◽  
Identification And Characterization

scholarly journals A new type of anvil in the Acheulian of Gesher Benot Ya‘aqov, Israel

2015 ◽  
Vol 370 (1682) ◽  
pp. 20140353 ◽  
Author(s):  
Naama Goren-Inbar ◽  
Gonen Sharon ◽  
Nira Alperson-Afil ◽  
Gadi Herzlinger
Keyword(s):  
Acquisition Strategies ◽  
Natural Geometry ◽  
Range Of Functions ◽  
Nut Cracking ◽  
New Type ◽  
Identification And Characterization ◽  
Selection Of

We report here on the identification and characterization of thin basalt anvils, a newly discovered component of the Acheulian lithic inventory of Gesher Benot Ya‘aqov (GBY). These tools are an addition to the array of percussive tools (percussors, pitted stones and anvils) made of basalt, flint and limestone. The thin anvils were selected from particularly compact, horizontally fissured zones of basalt flows. This type of fissuring produces a natural geometry of thick and thin slabs. Hominins at GBY had multiple acquisition strategies, including the selection of thick slabs for the production of giant cores and cobbles for percussors. The selection of thin slabs was carried out according to yet another independent and targeted plan. The thinness of the anvils dictated a particular range of functions. The use of the anvils is well documented on their surfaces and edges. Two main types of damage are identified: those resulting from activities carried out on the surfaces of the anvils and those resulting from unintentional forceful blows ( accidents de travaille ). Percussive activities that may have been associated with the thin anvils include nut cracking and the processing of meat and bones, as well as plants.

scholarly journals ANTIFUNGAL ACTIVITY OF ACTINOBACTERIA WITH A POTENTIAL TO INHIBIT RICE BLAST FUNGUS MAGNAPORTHE ORYZAE (ANAMORPH PYRICULARIA ORYZAE)

2021 ◽  
pp. 121-125
Author(s):  
THILAGAM R ◽  
BALAGURUNATHAN R ◽  
SANGEETHA M ◽  
HEMALATHA N
Keyword(s):  
Secondary Metabolites ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Biochemical Characterization ◽  
Fungal Growth ◽  
Pathogenic Fungi ◽  
Antifungal Compound ◽  
Layer Chromatography ◽  
Selection Of

Objective: The aims of the present study were to screen the actinobacteria with high potential ability to produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Magnaporthe oryzae. Production of secondary metabolites was analysis by thin-layer chromatography and bioautography assay. Methods: Screening and selection of potential Streptomyces sp. morphological, cultural, physiological, and biochemical characterization of the screened isolate was carried out. Antifungal compound was confirmed by bioautography assay. Results: Bioautography method use in this study was found to be antifungal fraction from the crude extract. Antifungal secondary metabolites can be readily located on the plates by observing clear zones where active compounds inhibit fungal growth. Conclusion: The bioautography assay shows that this isolates can produce antifungal compound. Therefore, this isolate proves to be a promising microbe which can be further studied for its applications a biocontrol agent against rice blast fungi.

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