scholarly journals 19-P025 Bone dynamics during adult zebrafish caudal fin regeneration

2009 ◽  
Vol 126 ◽  
pp. S298
Author(s):  
Sara Sousa ◽  
Nuno Afonso ◽  
Mariana Fonseca ◽  
António Jacinto
Keyword(s):  
Adult Zebrafish ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Bone Dynamics

scholarly journals Impact of hypoxia induced VEGF and its signaling during caudal fin regeneration in Zebrafish

2017 ◽  
Author(s):  
sagayaraj.R Vivek ◽  
R. Malathi
Keyword(s):  
Wound Healing ◽  
Blood Vessel ◽  
Cobalt Chloride ◽  
Adult Zebrafish ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Blood Vessel Formation ◽  
Vessel Formation ◽  
Vegf Signaling ◽  
Fin Length

ABSTRACT:Hypoxia is known to play important role during various cellular process, including regeneration. Regeneration is a complex process involving wound healing and tissue repair. We propose that hypoxia might mediate regeneration through angiogenesis involving angiogenic factors such as VEGF, VEGF-R2, NRP1a during the wound healing process. We have chosen Zebrafish model to study the role of hypoxia induced regeneration. Unlike mammals Zebrafish has the ability to regenerate. Hypoxic condition was mimicked using inorganic salt cobalt chloride to study caudal fin regeneration in adult Zebrafish. Intense blood vessel formation, with increased tail fin length experimented at various time points have been observed when adult zebrafish caudal fin partially amputated were exposed to 1% CoCl2. Regeneration is enhanced under hypoxia, with increased VEGF expression. To study the significance of VEGF signaling during wound healing and tissue regeneration, sunitinib well known inhibitor of VEGF receptor is used against CoCl2-induced caudal fin regeneration. Diminished fin length, lowering of blood vessel formation was documented using angioquant software, reduction in mRNA level of hypoxia inducible factors, VEGF and other pro-angiogenic genes such as VEGF, VEGF-R2, NRP1A, FGFR2, ANGPT1 were observed, while reduction in VEGF protein was demonstrated using western blot analysis. Genistein inhibitor of HIF-1α completely arrested regeneration, with suppression of VEGF highlighting the significance of hypoxia induced VEGF signaling during fin regeneration. Our results suggest that hypoxia through HIF-1α might lead to angiogenesis involving VEGF signaling during wound healing and this might throw light on therapeutic efficacy of cobalt chloride during regeneration.

scholarly journals Suppressive effects of valproic acid on caudal fin regeneration in adult zebrafish

2020 ◽  
Vol 24 (6) ◽  
pp. 349-358
Author(s):  
Yunkyoung Lee ◽  
Dohee Kim ◽  
Chang-Joong Lee
Keyword(s):  
Valproic Acid ◽  
Adult Zebrafish ◽  
Caudal Fin ◽  
Fin Regeneration

scholarly journals Two Different Transgenes to Study Gene Silencing and Re-Expression During Zebrafish Caudal Fin and Retinal Regeneration

2006 ◽  
Vol 6 ◽  
pp. 65-81 ◽  
Author(s):  
Ryan Thummel ◽  
Christopher T. Burket ◽  
David R. Hyde
Keyword(s):  
Tissue Regeneration ◽  
Transgene Expression ◽  
Egfp Expression ◽  
Transgenic Zebrafish ◽  
Retinal Regeneration ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Differentiated Cells ◽  
Neuronal Progenitors ◽  
Wound Epithelium

We used the 500-bpXenopusef1-α promoter and the 2-kb zebrafish histone2A.F/Zpromoter to generate several independent transgenic zebrafish lines expressing EGFP. While both promoters drive ubiquitous EGFP expression in early zebrafish development, they are systematically silenced in several adult tissues, including the retina and caudal fin. However, EGFP expression is temporarily renewed in the adult during either caudal fin or retinal regeneration. In the Tg(H2A.F/Z:EGFP)ntline, EGFP is moderately expressed in both the wound epithelium and blastema of the regenerating caudal fin. In the Tg(ef1-α:EGFP)ntline, EGFP expression is reinitiated and restricted to the blastema of the regenerating caudal fin and colabels with BrdU, PCNA, andmsxc-positive cells. Thus, these two ubiquitous promoters drive EGFP transgene expression in different cell populations during caudal fin regeneration. We further analyzed the ability of theef1-α:EGFPtransgene to label nonterminally differentiated cells during adult tissue regeneration. First, we demonstrated that the transgene is highly methylated in adult zebrafish caudal fin tissue, but not during fin regeneration, implicating methylation as a potential means of transgene silencing in this line. Next, we determined that theef1-α:EGFPtransgene is also re-expressed during adult retinal regeneration. Specifically, theef1-α:EGFPtransgene colabels with PCNA in the Müglia, a specialized cell that is the source of neuronal progenitors during zebrafish retinal regeneration. Thus, we concluded that Tg(ef1-α:EGFP)nt line visually marks nonterminally differentiated cells in multiple adult regeneration environments and may prove to be a useful marker in tissue regeneration studies in zebrafish.

Vitamin C supplementation improves growth performance and caudal fin regeneration in zebrafish ( Danio rerio )

2021 ◽  
Author(s):  
Pedro Luiz Pucci Figueiredo Carvalho ◽  
Pedro Henrique Ventura Almeida ◽  
William dos Santos Xavier ◽  
Igor Simões Tiagua Vicente ◽  
Matheus Gardim Guimarães ◽  
...  
Keyword(s):  
Vitamin C ◽  
Growth Performance ◽  
Danio Rerio ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Vitamin C Supplementation

scholarly journals Regrowth of zebrafish caudal fin regeneration is determined by the amputated length

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Toshiaki Uemoto ◽  
Gembu Abe ◽  
Koji Tamura
Keyword(s):  
Caudal Fin ◽  
Fin Regeneration

scholarly journals Novel Heparin Receptor Transmembrane Protein 184a Regulates Angiogenesis in the Adult Zebrafish Caudal Fin

2017 ◽  
Vol 8 ◽  
Author(s):  
Sara Lynn N. Farwell ◽  
Kimberly G. Reylander ◽  
M. Kathryn Iovine ◽  
Linda J. Lowe-Krentz
Keyword(s):  
Transmembrane Protein ◽  
Adult Zebrafish ◽  
Caudal Fin

scholarly journals Cellular diversity of the regenerating caudal fin

2020 ◽  
Vol 6 (33) ◽  
pp. eaba2084
Author(s):  
Yiran Hou ◽  
Hyung Joo Lee ◽  
Yujie Chen ◽  
Jiaxin Ge ◽  
Fujr Osman Ibrahim Osman ◽  
...  
Keyword(s):  
State Transitions ◽  
Specific Cell ◽  
Caudal Fin ◽  
Wound Site ◽  
Fin Regeneration ◽  
Differentiated Cells ◽  
Mesenchymal Differentiation ◽  
Cell Type Specific ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

Zebrafish faithfully regenerate their caudal fin after amputation. During this process, both differentiated cells and resident progenitors migrate to the wound site and undergo lineage-restricted, programmed cellular state transitions to populate the new regenerate. Until now, systematic characterizations of cells comprising the new regenerate and molecular definitions of their state transitions have been lacking. We hereby characterize the dynamics of gene regulatory programs during fin regeneration by creating single-cell transcriptome maps of both preinjury and regenerating fin tissues at 1/2/4 days post-amputation. We consistently identified epithelial, mesenchymal, and hematopoietic populations across all stages. We found common and cell type–specific cell cycle programs associated with proliferation. In addition to defining the processes of epithelial replenishment and mesenchymal differentiation, we also identified molecular signatures that could better distinguish epithelial and mesenchymal subpopulations in fish. The insights for natural cell state transitions during regeneration point to new directions for studying this regeneration model.

scholarly journals Swimming capability of zebrafish is governed by water temperature, caudal fin length and genetic background

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yuma Wakamatsu ◽  
Kazutoyo Ogino ◽  
Hiromi Hirata
Keyword(s):  
Water Temperature ◽  
Genetic Background ◽  
Swimming Performance ◽  
Water Velocity ◽  
Critical Swimming Speed ◽  
Adult Zebrafish ◽  
Caudal Fin ◽  
Behavioral Traits ◽  
Maximum Water ◽  
Fin Length

Abstract Several zebrafish strains such as AB, Tübingen (TU), Wild India Kolkata (WIK) and Tupfel long fin (TL) have been established for genetic study. Each strain has its morphological and behavioral traits. Motor traits, however, have not been explored in zebrafish strains. We here applied a treadmill for fish (swimmill) and measured swimming capability of adult zebrafish by critical swimming speed, which is the maximum water velocity in which fish can keep swimming. First, we confirmed that swimming capability does not vary between female and male. Second, we found that the appropriate water temperature for swimming was between 16 and 30 °C. Third, our fin clip experiments using long-finned zebrafish revealed that they can exhibit high swimming capability when the caudal fin length was set between 3 and 10 mm, implying that long-finned zebrafish are unfavorable for fast swimming. Finally, we compared swimming capability of several zebrafish strains and demonstrated that WIK fish was significantly less capable of swimming despite that they have short caudal fin (~9 mm). The offspring of WIK fish were less capable of swimming, while hybrids of WIK and TU showed high swimming performance comparable to TU. Thus, lower swimming capability of WIK strain is inheritable as a motor trait.

scholarly journals Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish

2017 ◽  
Vol 114 (5) ◽  
pp. E717-E726 ◽  
Author(s):  
Jeremy S. Rabinowitz ◽  
Aaron M. Robitaille ◽  
Yuliang Wang ◽  
Catherine A. Ray ◽  
Ryan Thummel ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Differential Expression ◽  
Spatial Information ◽  
Expression Patterns ◽  
Spatial Position ◽  
Adult Zebrafish ◽  
Cellular Functions ◽  
Caudal Fin ◽  
Functional Studies

Regeneration requires cells to regulate proliferation and patterning according to their spatial position. Positional memory is a property that enables regenerating cells to recall spatial information from the uninjured tissue. Positional memory is hypothesized to rely on gradients of molecules, few of which have been identified. Here, we quantified the global abundance of transcripts, proteins, and metabolites along the proximodistal axis of caudal fins of uninjured and regenerating adult zebrafish. Using this approach, we uncovered complex overlapping expression patterns for hundreds of molecules involved in diverse cellular functions, including development, bioelectric signaling, and amino acid and lipid metabolism. Moreover, 32 genes differentially expressed at the RNA level had concomitant differential expression of the encoded proteins. Thus, the identification of proximodistal differences in levels of RNAs, proteins, and metabolites will facilitate future functional studies of positional memory during appendage regeneration.

Role of Hox genes in zebrafish caudal fin regeneration

2006 ◽  
Vol 25 ◽  
pp. S25-S25
Author(s):  
M SARRASJR ◽  
R THUMMEL ◽  
A GODMAN
Keyword(s):  
Hox Genes ◽  
Caudal Fin ◽  
Fin Regeneration
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