Protease-resistant human GAD-derived altered peptide ligands decrease TNF-α and IL-17 production in peripheral blood cells from patients with type 1 diabetes mellitus

ObjectiveWe examined the relationship of inflammatory and endothelial dysfunction markers with the prevalence and incidence of gross proteinuria (GP) in persons with type 1 diabetes mellitus.DesignA longitudinal population-based cohort of persons with type 1 diabetes mellitus was followed from 1990–1992 through 2005–2007.MethodsPrevalence and 15-year cumulative incidence of GP were defined as outcome variables. Serum high-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), soluble vascular cell adhesion molecule-1 (VCAM-1), soluble intercellular adhesion molecule-1, and serum total homocysteine were measured. Multivariate logistic and discrete linear logistic regression modeling was used for data analysis.ResultsAfter controlling for duration of diabetes and other confounding factors, TNF-α (odds ratio (OR) 3.64; 95% confidence interval (CI) 2.33, 5.70), IL-6 (OR 1.41; 95% CI 1.06, 1.88), VCAM-1 (OR 13.35; 95% CI 5.39, 33.07), and homocysteine (OR 2.98; 95% CI 1.73, 5.16) were associated with prevalent proteinuria. Only hsCRP (OR 1.47; 95% CI 1.02, 2.11) was associated with incident proteinuria.ConclusionsThese findings suggest a role of inflammation and endothelial dysfunction as markers and contributors of the development of diabetic nephropathy in persons with type 1 diabetes mellitus.

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Studying Studying the relationship between Dipeptidyl peptidase-4 enzyme level, Tumor necrosis factor alpha enzyme level, and insulin resistance in type 1 Diabetes Mellitus patients

QJM ◽

10.1093/qjmed/hcaa052.042 ◽

2020 ◽

Vol 113 (Supplement_1) ◽

Author(s):

H M Amer ◽

M M M Mahdy ◽

W E Saad ◽

M N A Abdelsalam

Keyword(s):

Diabetes Mellitus ◽

Type 1 Diabetes ◽

Serum Level ◽

Type 1 Diabetes Mellitus ◽

Enzyme Level ◽

Venous Blood ◽

P Value ◽

Tnf Α ◽

The Relationship

Abstract Background Type 1 diabetes mellitus (T1DM) is an inflammatory disease of the pancreatic islets that results in absolute insulin deficiency and the consecutive hyperglycemia due to immune-mediated beta cell destruction throughout the globe, the incidence of T1DM is increasing at 3% to 5% per year Purpose To study the relationship between DPP-4 serum level, TNF a concentration measurements, and insulin sensitivity in T1DM patient Patients and Methods Patients were selected from the inpatient department of National Institute of Diabetes and Endocrinology during Januaray 2017, venous blood samples were collected to measure Serum level of creatinine, C-peptide, HbA1c%, DPP-4, TNFα . Measurement of eGDR was done using the formula 24.31 - 12.2 x (WHR) – 3.29 x (BLP) – 0.57 X HbA1c Study Design Cross sectional study that included 80 patients with type 1 Diabetes, Results The study population was divided according to median eGDR value of 6.125 mg kg-1 min-1 The group with higher IR(eGDR≤ 6.125)had higher value of DPP-4 enzyme level (1127.3 ± 40.9 ng/ml vs. 870.6 ± 30.2 ng/ml, p < 0.001) and TNF α concentration (11.4 ± 4.8 pg/ml vs 5.3 ± 1.6 pg/ml, p < 0.001). TNF α concentration negatively correlated with eGDR (r= -0.515, p value <0.001) DPP-4 level negatively correlated with eGDR (r= -0.826 and p value <0.001) while DPP-4 was positively correlated with TNF α (r = 0.441 p value ≤ 0.001)

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