Cellular uptake of modified oligonucleotides enhanced by porphyrins studied by time-resolved microspectrofluorimetry and fluorescence imaging techniques

2011 ◽  
Vol 993 (1-3) ◽  
pp. 316-318 ◽  
Author(s):  
P. Praus ◽  
E. Kočišová ◽  
P. Mojzeš ◽  
J. Štěpánek ◽  
P.-Y. Turpin ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cellular Uptake ◽  
Imaging Techniques ◽  
Modified Oligonucleotides ◽  
Time Resolved

Time-resolved Microspectrofluorometry and Fluorescence Imaging Techniques: Study of Porphyrin-mediated Cellular Uptake of Oligonucleotides

2008 ◽  
Vol 1130 (1) ◽  
pp. 117-121 ◽  
Author(s):  
Petr Praus ◽  
Eva Kočišová ◽  
Peter Mojzeš ◽  
Josef Štěpánek ◽  
Olivier Seksek ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Cellular Uptake ◽  
Imaging Techniques ◽  
Time Resolved

scholarly journals Study of Cellular Uptake of Modified Oligonucleotides by Using Time-Resolved Microspectrofluorimetry and Florescence Imaging

2012 ◽  
Vol 27 ◽  
pp. 415-419 ◽  
Author(s):  
P. Praus ◽  
E. Kočišová ◽  
P. Mojzeš ◽  
J. Štěpánek ◽  
F. Sureau
Keyword(s):  
Cell Line ◽  
Cellular Uptake ◽  
Fluorescence Lifetime ◽  
Antisense Oligonucleotide ◽  
Malignant Cell ◽  
Lifetime Data ◽  
Modified Oligonucleotides ◽  
Mcf7 Cells ◽  
Time Resolved ◽  
Modified Oligonucleotide

Fluorescence microimaging and homodyne phase-resolved confocal microspectrofluorimetry were used to monitor the transport of antisense oligonucleotide into cancer MCF7 cells and the subsequent intracellular distribution. Phosphorothioate analog of 15-mer oligoadenylate (dA15) labeled by ATTO 425 was complexed with 5,10,15,20-tetrakis (1-methyl-4-pyridyl) porphyrin (H2TMPyP4) as an uptake-mediating agent. Fluorescence lifetime data within a broad spectral range have revealed properties of both components inside the cell. H2TMPyP4 lifetime inside the cell is not influenced in this malignant cell line, while the lifetime of modified oligonucleotide was found to be slightly shortened.

scholarly journals Lateral Distribution of the Transmembrane Domain of Influenza Virus Hemagglutinin Revealed by Time-resolved Fluorescence Imaging

2009 ◽  
Vol 284 (23) ◽  
pp. 15708-15716 ◽  
Author(s):  
Silvia Scolari ◽  
Stephanie Engel ◽  
Nils Krebs ◽  
Anna Pia Plazzo ◽  
Rodrigo F. M. De Almeida ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Fluorescence Imaging ◽  
Transmembrane Domain ◽  
Lateral Distribution ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Influenza Virus Hemagglutinin

Time-resolved pulsed-plasma characterization using broadband terahertz pulses correlated with fluorescence imaging

2005 ◽  
Vol 87 (15) ◽  
pp. 151501 ◽  
Author(s):  
B. H. Kolner ◽  
P. M. Conklin ◽  
R. A. Buckles ◽  
N. K. Fontaine ◽  
R. P. Scott
Keyword(s):  
Fluorescence Imaging ◽  
Pulsed Plasma ◽  
Time Resolved ◽  
Terahertz Pulses ◽  
Plasma Characterization ◽  
Broadband Terahertz

scholarly journals Fluorescence imaging with tailored light

Nanophotonics ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 2111-2128 ◽  
Author(s):  
Jialei Tang ◽  
Jinhan Ren ◽  
Kyu Young Han
Keyword(s):  
Fluorescence Imaging ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Optical Parameters ◽  
Propagation Angle ◽  
Resolution Imaging ◽  
Time Observation ◽  
Living Organisms ◽  
Real Time Observation

AbstractFluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization, and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.

scholarly journals 3D Printing and NIR Fluorescence Imaging Techniques for the Fabrication of Implants

Materials ◽  
2020 ◽  
Vol 13 (21) ◽  
pp. 4819
Author(s):  
Yong Joon Suh ◽  
Tae Hyeon Lim ◽  
Hak Soo Choi ◽  
Moon Suk Kim ◽  
Sang Jin Lee ◽  
...  
Keyword(s):  
3D Printing ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging Techniques ◽  
The Body ◽  
Monitoring Method ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Nir Fluorescence ◽  
Printing Techniques

Three-dimensional (3D) printing technology holds great potential to fabricate complex constructs in the field of regenerative medicine. Researchers in the surgical fields have used 3D printing techniques and their associated biomaterials for education, training, consultation, organ transplantation, plastic surgery, surgical planning, dentures, and more. In addition, the universal utilization of 3D printing techniques enables researchers to exploit different types of hardware and software in, for example, the surgical fields. To realize the 3D-printed structures to implant them in the body and tissue regeneration, it is important to understand 3D printing technology and its enabling technologies. This paper concisely reviews 3D printing techniques in terms of hardware, software, and materials with a focus on surgery. In addition, it reviews bioprinting technology and a non-invasive monitoring method using near-infrared (NIR) fluorescence, with special attention to the 3D-bioprinted tissue constructs. NIR fluorescence imaging applied to 3D printing technology can play a significant role in monitoring the therapeutic efficacy of 3D structures for clinical implants. Consequently, these techniques can provide individually customized products and improve the treatment outcome of surgeries.

scholarly journals On-Site Monitoring of Postoperative Bile Leakage Using Bilirubin-Inducible Fluorescent Protein

2020 ◽  
Vol 44 (12) ◽  
pp. 4245-4253
Author(s):  
Yoshiharu Kono ◽  
Takeaki Ishizawa ◽  
Norihiro Kokudo ◽  
Yugo Kuriki ◽  
Ryu J. Iwatate ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Fluorescent Protein ◽  
Imaging Techniques ◽  
Abdominal Cavity ◽  
Bile Leakage ◽  
Indirect Bilirubin ◽  
Site Monitoring ◽  
Real Time Identification ◽  
Time Required ◽  
Bile Leaks

Abstract Background Bile leakage is the most common postoperative complication associated with hepatobiliary and pancreatic surgery. Until now, however, a rapid, accurate diagnostic method for monitoring intraoperative and postoperative bile leakage had not been established. Method Bilirubin levels in drained abdominal fluids collected from 23 patients who had undergone hepatectomy (n = 22) or liver transplantation (n = 1) were measured using a microplate reader with excitation/emission wavelengths of 497/527 nm after applying 5 µM of UnaG to the samples. UnaG was also sprayed directly on hepatic raw surfaces in swine hepatectomy models to identify bile leaks by fluorescence imaging. Results The bilirubin levels measured by UnaG fluorescence imaging showed favorable correlations with the results of the conventional light-absorptiometric methods (indirect bilirubin: rs = 0.939, p < 0.001; direct bilirubin: rs = 0.929, p < 0.001). Approximate time required for bilirubin measurements with UnaG was 15 min, whereas it took about 40 min with the conventional method at a hospital laboratory. Following administration of UnaG on hepatic surfaces, the fluorescence imaging identified bile leaks not only on the resected specimens but also in the abdominal cavity of the swine hepatectomy models. Conclusion Fluorescence imaging techniques using UnaG may enable real-time identification of bile leaks during hepatectomy and on-site rapid diagnosis of bile leaks after surgery.

Application of Indocyanine Green in Flap Surgery: A Systematic Review

2017 ◽  
Vol 34 (02) ◽  
pp. 077-086 ◽  
Author(s):  
Ke Li ◽  
Zheng Zhang ◽  
Fabio Nicoli ◽  
Christopher D'Ambrosia ◽  
Wenjing Xi ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Standard Dose ◽  
Imaging Techniques ◽  
Critical Factor ◽  
Flap Surgery ◽  
Tissue Flaps ◽  
Transfer Procedures ◽  
Abstract Screening

Background The vascularization of the distal portions of transferred tissue represents the most critical factor in the success of reconstructive surgery. In recent years, indocyanine green (ICG) fluorescence imaging techniques have been applied during surgery to evaluate flap perfusion. However, this investigation has found that there is little consensus regarding the standard dose of ICG as well as the pre-operative requirements of ICG allergy testing. The aim of this study is to summarize the applications of ICG to tissue transfers and safe dosing practices and to provide insight to the possible adverse effects of ICG on flap surgery with the goal of helping clinicians apply ICG safely and efficiently to tissue transfer procedures. Methods A literature search was performed using, Wiley InterScience, and Springer with the key words, ‘Flap,’ ‘indocyanine green,’ ‘surgery,’ and related mesh words for all publications between 2005 and 2015. Title and abstract screening was performed using predefined in- and exclusion criteria. Results Seventy-three articles were included. These were classified as “application of ICG in flap surgery” and “the security of applying ICG in flap surgery”. Conclusions ICG fluorescence imaging preoperatively facilitates the detection of perforators in tissue flaps with thickness <20 mm, aids in the evaluation of flap microcirculation and perfusion, and allows surgeons to select dominant cutaneous nerves while evaluating the quality of vascular anastomoses and locating thromboses. The literature also concluded that potential allergic reactions to ICG should be taken into consideration.

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