A biocompatible glue consisting of human serum albumin (HSA) and citric acid
derivative (CAD), named CAD-A glue was developed. CAD was successfully synthesized by the
reaction between citric acid and N-hydroxysuccinimide in the presence of 1-ethyl-3-(3-dimethyl
aminopropyl) carbodiimide hydrochloride. When the CAD-A glue was applied to the collagenbased
casings, it took 7 min to obtain half of maximum bonding strength (760 g/cm2). The bonding
strength of this glue to collagen-based casings increased with increasing of HSA concentration.The
bonding strength of CAD-A glue increased with increasing CAD concentration up to 200 mM, and
then decreased with increasing CAD concentration under the fixed HSA concentration (50 w/w%).
The CAD-A glue showed excellent wound closure ability rather than fibrin glue when applied to the
mouse skin. These results suggested that this developed glue had both tissue compatibility and
bonding strength for use in clinical field.