PEGylated hollow gold nanoparticles for combined X-ray radiation and photothermal therapy in vitro and enhanced CT imaging in vivo

The treatment of malignant bone tumors (including primary bone tumors and metastatic bone tumors) has always been a clinical challenge. The purpose of this study is to design a bone-targeted nano-carrier with photothermal effect to achieve chemo-photothermal therapy (CPT), which allows the minimal use of photothermal agents and chemical drugs to target bone tumors. Alendronate modified hollow gold nanoparticles (HGNPs- ALN) were synthesized using mercapto polyethylene glycol carboxyl (SH-PEG2000-COOH) as the connecting arm, fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM) data show that HGNPs-ALN with a particle size of about 80 nm has been successfully synthesized. The hydroxyapatite affinity experiment in vitro indicated that HGNPs-ALN exhibited a high affinity to bone. In addition, the temperature of HGNPs-ALN under near-infrared laser irradiation can rise to 53 °C, which can achieve effective photothermal therapy for bone tumors. Bone-targeted hollow gold nanoparticles (DOX@HGNPs-ALN) loaded with doxorubicin hydrochloride (DOX) were synthesized by one-pot method. By comparing the stability and drug loading of HGNPs-ALN, it was concluded that the optimal mass ratio of HGNPs-ALN (calculated by the amount of gold) to DOX was about 1:2. HGNPs-ALN and DOX@HGNPs-ALN both have good photothermal stability and photothermal transformation properties, and confirmed the safety of HGNPs on human osteosarcoma cells. MTT experiments showed that DOX@HGNPs-ALN had the strongest killing effect on MG-63 osteosarcoma cells under laser irradiation (the killing rate is about 65%). According to these results, it can be considered that DOX@HGNPs-ALN has the potential of CPT synergistic targeting therapy for bone tumors.

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P0131 Efficacy of gum arabic-conjugated gold nanoparticles as a photothermal therapy for lung cancer: In vitro and in vivo approaches

European Journal of Cancer ◽

10.1016/j.ejca.2014.03.175 ◽

2014 ◽

Vol 50 ◽

pp. e46 ◽

Cited By ~ 1

Author(s):

A.M. Gamal-Eldeen ◽

D. Moustafa ◽

S.M. El-Daly ◽

K.V. Katti

Keyword(s):

Lung Cancer ◽

Gold Nanoparticles ◽

Photothermal Therapy ◽

Gum Arabic

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Untrasmall Bi2S3 nanodots for in vivo X-ray CT imaging-guided photothermal therapy of cancer

RSC Advances ◽

10.1039/c7ra04132b ◽

2017 ◽

Vol 7 (47) ◽

pp. 29672-29678 ◽

Cited By ~ 11

Author(s):

Zelun Li ◽

Kelong Ai ◽

Zhe Yang ◽

Tianqi Zhang ◽

Jianhua Liu ◽

...

Keyword(s):

Cancer Treatment ◽

Real Time ◽

Photothermal Therapy ◽

Therapeutic Response ◽

Ct Imaging ◽

X Ray

Theranostic nanomedicine has shown tremendous promise for more effective and predictive cancer treatment by real-time mornitoring of the delivery of therapeutics to tumors and subsequent therapeutic response.

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Synthesis, characterization, biological activities, and catalytic applications of alcoholic extract of saffron (Crocus sativus) flower stigma-based gold nanoparticles

Green Processing and Synthesis ◽

10.1515/gps-2021-0024 ◽

2021 ◽

Vol 10 (1) ◽

pp. 230-245

Author(s):

Fahad A. Alhumaydhi ◽

Ibrahim Khan ◽

Abdur Rauf ◽

Muhammad Nasimullah Qureshi ◽

Abdullah S. M. Aljohani ◽

...

Keyword(s):

Gold Nanoparticles ◽

Biological Activities ◽

Inhibitory Effect ◽

Biological Properties ◽

Crocus Sativus ◽

Alcoholic Extract ◽

X Ray ◽

Vis Spectroscopy

Abstract Currently, nanotechnology is gaining massive attention compared to conventional methods as the biosynthesis of plant-based nanoparticles is considered safe, effective, and ecofriendly. Therefore, keeping in view the importance of nanotechnology, the present study was designed to synthesize, characterize, and evaluate the biological effectiveness of saffron stigma-based gold nanoparticles (SS-AuNPs) for their in vitro and in vivo biological properties. These gold nanoparticles were characterized by UV–Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). The highest antibacterial effect was observed by the saffron extract against Escherichia coli (22 mm). SS-AuNPs significantly inhibited the activity of enzyme urease (54.98%) and CA-II (64.29%). However, the nonsignificant inhibitory effect was observed in the case of α-chymotrypsin. Maximum analgesic (84.98%) and antiinflammatory (88.98%) effects were observed for SS-AuNPs (10 mg/kg). Similarly, SS-AuNPs demonstrated a significant (P < 0.01) sedative effect at all tested doses.

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Synthesis of PEG-Iodine-Capped Gold Nanoparticles and Their Contrast Enhancement inIn VitroandIn Vivofor X-Ray/CT

Journal of Nanomaterials ◽

10.1155/2012/504026 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

Sun-Hee Kim ◽

Eun-Mi Kim ◽

Chang-Moon Lee ◽

Dong Wook Kim ◽

Seok Tae Lim ◽

...

Keyword(s):

Gold Nanoparticles ◽

Contrast Enhancement ◽

Energy Shift ◽

Blood Pool ◽

Ct Imaging ◽

Binding Energies ◽

X Ray ◽

Ct Contrast Agent ◽

Liver And Kidney

We designed gold nanoparticles (AuNPs) capped with iodine and polyethylene glycol (PEG) to provide effective enhancement for X-ray CT imaging. The methoxy PEG-iodine-capped AuNPs were prepared through the chemisorption of iodine and substitution of methoxy PEG-SH onto the surface of gold nanoparticles, and severe aggregation in TEM was not observed. The binding energies of Au 4f7/2and I 3d5/2of the methoxy PEG-iodine-capped AuNPs were obtained as 84.1 eV and 619.3 eV, respectively. The binding energy shift of methoxy PEG-iodine-capped AuNPs would be resulted from the chemisorption between gold nanoparticles and iodine atoms. The methoxy PEG-iodine-capped AuNPs have higher enhancement compared to PEG-capped gold nanoparicles in the same amount of goldin vitro. After postinjection of methoxy PEG-iodine-capped AuNPs into the mice, dramatic contrast enhancement at the heart, aorta, liver, and kidney was observed, this was maintained up to 5 days, and there was no evidence of apparent toxicity. In conclusion, methoxy PEG-iodine-capped AuNPs might be a good candidate as a CT contrast agent for blood pool imaging, and this will also contribute to the prolongation of a blood circulation time for X-ray CT imaging.

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Engineered Exosomes-Based Photothermal Therapy with MRI/CT Imaging Guidance Enhances Anticancer Efficacy through Deep Tumor Nucleus Penetration

Pharmaceutics ◽

10.3390/pharmaceutics13101593 ◽

2021 ◽

Vol 13 (10) ◽

pp. 1593

Author(s):

Min Yang ◽

Xiaohui Wang ◽

Fang Pu ◽

Ying Liu ◽

Jia Guo ◽

...

Keyword(s):

Photothermal Therapy ◽

Tumor Therapy ◽

Ct Imaging ◽

Cancer Site ◽

Control Group ◽

Anticancer Efficacy ◽

In Vivo Experiments ◽

Actual Application

Exosomes, as natural nanovesicles, have become a spotlight in the field of cancer therapy due to their reduced immunogenicity and ability to overcome physiological barriers. However, the tumor targeting ability of exosomes needs to be improved before its actual application. Herein, a multiple targeted engineered exosomes nanoplatform was constructed through rare earth element Gd and Dy-doped and TAT peptide-modified carbon dots (CDs:Gd,Dy-TAT) encapsulated into RGD peptide engineered exosomes (Exo-RGD), which were used to enhance the effect of cancer imaging diagnosis and photothermal therapy. In vitro and in vivo experiments showed that the resulting CDs:Gd,Dy-TAT@Exo-RGD could effectively accumulate at cancer site with an increased concentration owing to the targeting peptides modification and exosomes encapsulation. The tumor therapy effects of mice treated with CDs:Gd,Dy-TAT@Exo-RGD were heightened compared with mice from the CDs:Gd,Dy control group. After intravenous injection of CDs:Gd,Dy-TAT@Exo-RGD into tumor-bearing mice, the temperature of tumors rose to above 50 °C under NIR irradiation and the localized hyperpyrexia induced by CDs could remarkably ablate tumors. The survival rate of the mice was 100% after 60 days. In addition, the CDs:Gd,Dy-TAT@Exo-RGD exhibited higher MRI/CT imaging contrast enhancement of tumor sites than that of CDs:Gd,Dy. Our study identified that engineered exosomes are a powerful tool for encapsulating multiple agents to enhance cancer theranostic efficiency and provide insight into precise personalized nanomedicine.

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Ultrasmall PEI-Decorated Bi2Se3 Nanodots as a Multifunctional Theranostic Nanoplatform for in vivo CT Imaging-Guided Cancer Photothermal Therapy

Frontiers in Pharmacology ◽

10.3389/fphar.2021.795012 ◽

2021 ◽

Vol 12 ◽

Author(s):

Peng Zhang ◽

Lei Wang ◽

Xiuying Chen ◽

Xiang Li ◽

Qinghai Yuan

Keyword(s):

Photothermal Therapy ◽

Room Temperature ◽

Near Infrared ◽

Infrared Region ◽

Ct Imaging ◽

Broad Absorption ◽

Good Biocompatibility ◽

Imaging Performance

Bi-based nanomaterials, such as Bi2Se3, play an important part in biomedicine, such as photothermal therapy (PTT) and computed tomography (CT) imaging. Polyethylenimine (PEI)-modified ultrasmall Bi2Se3 nanodots were prepared using an ultrafast synthetic method at room temperature (25°C). Bi2Se3 nanodots exhibited superior CT imaging performance, and could be used as effective photothermal reagents owing to their broad absorption in the ultraviolet–visible–near infrared region. Under irradiation at 808 nm, PEI-Bi2Se3 nanodots exhibited excellent photothermal-conversion efficiency of up to 41.3%. Good biocompatibility and significant tumor-ablation capabilities were demonstrated in vitro and in vivo. These results revealed that PEI-Bi2Se3 nanodots are safe and a good nanotheranostic platform for CT imaging-guided PTT of cancer.

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Effect of Flavonoid-Coated Gold Nanoparticles on Bacterial Colonization in Mice Organs

Nanomaterials ◽

10.3390/nano10091769 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1769 ◽

Cited By ~ 1

Author(s):

Sundus Riaz ◽

Nosheen Fatima Rana ◽

Irshad Hussain ◽

Tahreem Tanweer ◽

Afrah Nawaz ◽

...

Keyword(s):

Gold Nanoparticles ◽

Bacterial Colonization ◽

X Ray ◽

Transmission Electron ◽

Nano Drug Delivery ◽

Vis Spectroscopy ◽

Scanning Transmission ◽

Synthesis Approach

Multidrug resistance (MDR) has been a potentiator for the exploration of antibiotics. Nano drug delivery systems have opened new avenues to overcome this challenge. Although antibacterial nanocarriers are extensively realized, their effect on the bacteria residing inside the tissues and their toxicity is rarely explored. This study investigated the effects of flavonoid coated gold nanoparticles (FAuNPs) on the colonization of Enterococcus faecalis in the mouse liver and kidneys. Flavonoids were extracted from the leaves of Berberis lycium Royle and used to stabilize gold following a green synthesis approach. FAuNPs were characterized by ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared spectroscopy (FTIR), scanning transmission electron microscopy (STEM), X-ray powder diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDS). FAuNPs showed significantly higher reduction in bacterial counts in in-vitro and in-vivo in mice organs as compared to the free flavonoids owing to their biocompatibility and effectiveness.

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Protein sulfenic acid-mediated anchoring of gold nanoparticles for enhanced CT imaging and radiotherapy of tumors in vivo

Nanoscale ◽

10.1039/d0nr06440h ◽

2020 ◽

Vol 12 (45) ◽

pp. 22963-22969

Author(s):

Jianan Ding ◽

Qiulian Mao ◽

Meng Zhao ◽

Yinjia Gao ◽

Anna Wang ◽

...

Keyword(s):

Gold Nanoparticles ◽

Ct Imaging ◽

Sulfenic Acid ◽

Enhanced Ct

Herein, smart protein sulfenic acid-reactive gold nanoparticles were reported as effective radiosensitizers for CT imaging and radiotherapy of tumors. They enable on-site immobilization within tumors resulting in enhanced accumulation and retention.

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