Transcriptome profiling of wheat under heat and cold stress treatments

AbstractChanges in gene expression are important for response to abiotic stress. Transcriptome profiling performed on maize inbred and hybrid genotypes subjected to heat or cold stress identifies many transcript abundance changes in response to these environmental conditions. Motifs that are enriched near differentially expressed genes were used to develop machine learning models to predict gene expression responses to heat or cold. The best performing models utilize the sequences both upstream and downstream of the transcription start site. Prediction accuracies could be improved using models developed for specific co-expression clusters compared to using all up- or down-regulated genes or by only using motifs within unmethylated regions. Comparisons of expression responses in multiple genotypes were used to identify genes with variable response and to identify cis- or trans-regulatory variation. Models trained on B73 data have lower performance when applied to Mo17 or W22, this could be improved by using models trained on data from all genotypes. However, the models have low accuracy for correctly predicting genes with variable responses to abiotic stress. This study provides insights into cis-regulatory motifs for heat- and cold-responsive gene expression and provides a framework for developing models to predict expression response to abiotic stress across multiple genotypes.One sentence summaryTranscriptome profiling of maize inbred and hybrid seedlings subjected to heat or cold stress was used to identify key cis-regulatory elements and develop models to predict gene expression responses.

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BIOCLIMATES OF HEAT AND COLD STRESS IN NEW ZEALAND

Weather and Climate ◽

10.2307/44279738 ◽

1987 ◽

Vol 7 (2) ◽

pp. 55 ◽

Cited By ~ 9

Author(s):

de Freitas

Keyword(s):

New Zealand ◽

Cold Stress ◽

Heat And Cold Stress

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Methods of Evaluating Protective Clothing Relative to Heat and Cold Stress: Thermal Manikin, Biomedical Modeling, and Human Testing

Journal of Occupational and Environmental Hygiene ◽

10.1080/15459624.2011.613291 ◽

2011 ◽

Vol 8 (10) ◽

pp. 588-599 ◽

Cited By ~ 33

Author(s):

Catherine O’Brien ◽

Laurie A. Blanchard ◽

Bruce S. Cadarette ◽

Thomas L. Endrusick ◽

Xiaojiang Xu ◽

...

Keyword(s):

Cold Stress ◽

Protective Clothing ◽

Thermal Manikin ◽

Heat And Cold Stress ◽

Biomedical Modeling

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Overexpression of SgGH3.1 from Fine-Stem Stylo (Stylosanthes guianensis var. intermedia) Enhances Chilling and Cold Tolerance in Arabidopsis thaliana

Genes ◽

10.3390/genes12091367 ◽

2021 ◽

Vol 12 (9) ◽

pp. 1367

Author(s):

Ming Jiang ◽

Long-Long Ma ◽

Huai-An Huang ◽

Shan-Wen Ke ◽

Chun-Sheng Gui ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Cold Stress ◽

Stress Responses ◽

Molecular Mechanisms ◽

Transcriptome Profiling ◽

Pasture Legumes ◽

Cold Tolerant ◽

Whole Transcriptome ◽

Altered Sensitivity ◽

Exogenous Iaa

Stylosanthes (stylo) species are commercially significant tropical and subtropical forage and pasture legumes that are vulnerable to chilling and frost. However, little is known about the molecular mechanisms behind stylos’ responses to low temperature stress. Gretchen-Hagen 3 (GH3) proteins have been extensively investigated in many plant species for their roles in auxin homeostasis and abiotic stress responses, but none have been reported in stylos. SgGH3.1, a cold-responsive gene identified in a whole transcriptome profiling study of fine-stem stylo (S. guianensis var. intermedia) was further investigated for its involvement in cold stress tolerance. SgGH3.1 shared a high percentage of identity with 14 leguminous GH3 proteins, ranging from 79% to 93%. Phylogenetic analysis classified SgGH3.1 into Group Ⅱ of GH3 family, which have been proven to involve with auxins conjugation. Expression profiling revealed that SgGH3.1 responded rapidly to cold stress in stylo leaves. Overexpression of SgGH3.1 in Arabidopsis thaliana altered sensitivity to exogenous IAA, up-regulated transcription of AtCBF1-3 genes, activated physiological responses against cold stress, and enhanced chilling and cold tolerances. This is the first report of a GH3 gene in stylos, which not only validated its function in IAA homeostasis and cold responses, but also gave insight into breeding of cold-tolerant stylos.

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Transcriptome profiling in Camellia japonica var. decumbens for the discovery of genes involved in chilling tolerance under cold stress

Annals of Forest Research ◽

10.15287/afr.2018.1311 ◽

2019 ◽

Vol 62 (1) ◽

Author(s):

Yawen Wu ◽

Markus Müller ◽

Tian Bai ◽

Shunyang Yao ◽

Oliver Gailing ◽

...

Keyword(s):

Cold Stress ◽

Transcriptome Profiling ◽

Chilling Tolerance ◽

Camellia Japonica

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Correlation of Heat and Cold Tolerance in Iranian Tall Fescue Ecotypes with Reactive Oxygen Species Scavenging and Osmotic Adjustment

HortScience ◽

10.21273/hortsci13088-18 ◽

2018 ◽

Vol 53 (7) ◽

pp. 1062-1068

Author(s):

Mohamad-Hossein Sheikh-Mohamadi ◽

Nematollah Etemadi ◽

Mostafa Arab

Keyword(s):

Cold Stress ◽

Tall Fescue ◽

Osmotic Adjustment ◽

Festuca Arundinacea ◽

Antioxidant Activities ◽

Control Group ◽

Excessive Heat ◽

Total Nonstructural Carbohydrates ◽

Heat And Cold Stress

Excessive heat or cold usually reduces the growth and quality of turfgrass. Genetic variations along with efficient biochemical and physiological mechanisms can diversify the tolerance to heat and cold. This study examined the effects of heat and cold stress on several biochemical and physiological parameters in Iranian tall fescue ecotypes (Festuca arundinacea L.). The control group of plants was maintained under optimal temperatures, whereas other groups were exposed to heat or cold in a growth chamber. The experiment was designed as a split plot, with stress treatments as the main plots and ecotypes as subplots. Physiologically and biochemically, the results revealed that three ecotypes (‘FA1’, ‘FA3’, and ‘FA5’) of the eight ecotypes examined in this study had better abilities to survive the simulated heat and cold stress. Better tolerance to heat and cold in the ‘FA1’, ‘FA3’, and ‘FA5’ ecotypes were probably due to higher levels of enzymatic and nonenzymatic antioxidant activities, maintenance of lower levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2), higher levels of proline and total nonstructural carbohydrates (TNC), along with a more efficient osmotic adjustment. Diamine oxidase (DAO) and polyamine oxidase (PAO) activities increased significantly in ‘FA1’, ‘FA3’, and ‘FA5’ ecotypes. In summary, the strength of tolerance among ecotypes can be ranked as ‘FA1’ > ‘FA3’ > ‘FA5’ > ‘FA2’ > ‘FA6’ > ‘FA4’ > ‘FA7’ > ‘FA8’ under heat stress and ‘FA5’> ‘FA1’ > ‘FA3’ > ‘FA2’ > ‘FA4’ > ‘FA6’ > ‘FA7’ > ‘FA8’ under cold stress.

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Implementing effective animal-based measurements for assessing animal welfare on farms and slaughter plants.

Improving animal welfare: a practical approach ◽

10.1079/9781789245219.0060 ◽

2021 ◽

pp. 60-83

Author(s):

Temple Grandin

Keyword(s):

Cold Stress ◽

Animal Welfare ◽

Body Condition ◽

Critical Points ◽

Heat And Cold Stress ◽

Standards And Guidelines ◽

The Difference ◽

Set Up ◽

Animal Handling ◽

Input Resource

Abstract This chapter describes how to write clear animal welfare standards and guidelines that will be interpreted the same way by different people; the difference between animal-based outcome measures and input resource-based standards; how to determine the most important core criteria or critical points to prevent abuse or neglect; easy-to-use measures for assessing body condition, lameness, injuries, condition of haircoat/feathers, animal handling, hygiene, heat and cold stress and the presence of abnormal behaviour and how to set up effective animal welfare auditing programmes.

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Socio-Economic Impact of and Adaptation to Extreme Heat and Cold of Farmers in the Food Bowl of Nepal

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16091578 ◽

2019 ◽

Vol 16 (9) ◽

pp. 1578 ◽

Cited By ~ 1

Author(s):

Nanda Kaji Budhathoki ◽

Kerstin K. Zander

Keyword(s):

Cold Stress ◽

Economic Impact ◽

Heat Waves ◽

Labour Productivity ◽

Strong Association ◽

Productivity Loss ◽

Extreme Weather Events ◽

Common Source ◽

Adaptation Measures ◽

Heat And Cold Stress

Farmers worldwide have to deal with increasing climate variability and weather extremes. Most of the previous research has focused on impacts on agricultural production, but little is known about the related social and economic impacts on farmers. In this study, we investigated the social and economic impact of extreme weather events (EWE) on farmers in Nepal, and explored how they coped with and adapted to heat waves and cold spells between 2012 and 2017. To address these aims, we conducted a survey of 350 farms randomly selected from the Bardiya and Banke districts of the Terai lowlands of Nepal. They were specifically asked to rate the impacts of extreme temperatures, as well as their effect on labour productivity and collective farmer health, and the detailed preventative measures they had implemented. About 84% of the farmers self-reported moderate or severe heat stress during the last five years, and about 85%, moderate or severe cold stress. Likewise, the majority of respondents reported that both farmer health and labour productivity had been compromised by EWEs. Productivity loss had a strong association with the perceived levels of heat and cold stress, which, in turn, were more likely to be reported by farmers with previous EWE experience. Potentially due to the increased care required during EWEs, those farmers with livestock reported increased heat and cold stress, as, surprisingly, did those who had implemented adaptation measures. Farmers seemed to be less prepared for potential threats of cold spells than heat waves, and therefore less likely to adopt coping strategies, since these are a recent phenomenon. This study identified some limitations. The cross sectional and self-reported data, as a common source of information to estimate health impact, level of heat/cold stress and labour productivity loss. Community-based education/community engagement programs could be developed to facilitate proactive adaptation.

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Is there a Need to Integrate Human Thermal Models with Weather Forecasts to Predict Thermal Stress?

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16224586 ◽

2019 ◽

Vol 16 (22) ◽

pp. 4586 ◽

Cited By ~ 3

Author(s):

Petersson ◽

Kuklane ◽

Gao

Keyword(s):

Thermal Stress ◽

Cold Stress ◽

Extreme Weather ◽

Data Availability ◽

Extreme Weather Events ◽

Weather Data ◽

Stress Index ◽

Thermal Models ◽

Weather Events ◽

Heat And Cold Stress

More and more people will experience thermal stress in the future as the global temperature is increasing at an alarming rate and the risk for extreme weather events is growing. The increased exposure to extreme weather events poses a challenge for societies around the world. This literature review investigates the feasibility of making advanced human thermal models in connection with meteorological data publicly available for more versatile practices and a wider population. By providing society and individuals with personalized heat and cold stress warnings, coping advice and educational purposes, the risks of thermal stress can effectively be reduced. One interesting approach is to use weather station data as input for the wet bulb globe temperature heat stress index, human heat balance models, and wind chill index to assess heat and cold stress. This review explores the advantages and challenges of this approach for the ongoing EU project ClimApp where more advanced models may provide society with warnings on an individual basis for different thermal environments such as tropical heat or polar cold. The biggest challenges identified are properly assessing mean radiant temperature, microclimate weather data availability, integration and continuity of different thermal models, and further model validation for vulnerable groups.

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