Manganese transport via the transferrin mechanism

Streptococcus sanguinis is an oral commensal and an etiological agent of infective endocarditis. Previous studies have identified the SsaACB manganese transporter as essential for endocarditis virulence; however, the significance of SsaACB in the oral environment has never been examined. Here we report that a ΔssaACB mutant of strain SK36 exhibits reduced growth and manganese uptake under acidic conditions. Further studies revealed that these deficits resulted from the decreased activity of TmpA, shown in the accompanying paper to function as a ZIP-family manganese transporter. Transcriptomic analysis of fermentor-grown cultures of SK36 WT and ΔssaACB strains identified pH-dependent changes related to carbon catabolite repression in both strains, though their magnitude was generally greater in the mutant. In strain VMC66, which possesses a MntH transporter, loss of SsaACB did not significantly alter growth or cellular manganese levels under the same conditions. Interestingly, there were only modest differences between SK36 and its ΔssaACB mutant in competition with Streptococcus mutans in vitro and in a murine oral colonization model. Our results suggest that the heterogeneity of the oral environment may provide a rationale for the variety of manganese transporters found in S. sanguinis and point to strategies for enhancing the safety of oral probiotics.

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Manganese transport in the rat optic nerve evaluated with spatial- and time-resolved magnetic resonance imaging

Journal of Magnetic Resonance Imaging ◽

10.1002/jmri.22284 ◽

2010 ◽

Vol 32 (3) ◽

pp. 551-560 ◽

Cited By ~ 14

Author(s):

Øystein Olsen ◽

Anders Kristoffersen ◽

Marte Thuen ◽

Axel Sandvig ◽

Christian Brekken ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Optic Nerve ◽

Magnetic Resonance ◽

Resonance Imaging ◽

Time Resolved ◽

Manganese Transport

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Hormonal control of manganese transport in the mouse thyroid

Cellular and Molecular Life Sciences ◽

10.1007/bf01930470 ◽

1992 ◽

Vol 48 (3) ◽

pp. 262-265 ◽

Cited By ~ 8

Author(s):

M. Nishida ◽

J. Kawada

Keyword(s):

Hormonal Control ◽

Manganese Transport ◽

Mouse Thyroid

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Disorders of manganese transport

Rosenberg's Molecular and Genetic Basis of Neurological and Psychiatric Disease ◽

10.1016/b978-0-12-813955-4.00048-9 ◽

2019 ◽

pp. 643-655

Author(s):

Isaac Marin-Valencia ◽

Sidney M Gospe

Keyword(s):

Manganese Transport

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Treatable Hereditary Manganese Transport Disorder: Novel SLC30A10 Mutation and its Characteristic Neuroimaging Appearance in Two Siblings

Journal of Pediatric Genetics ◽

10.1055/s-0040-1713853 ◽

2020 ◽

Author(s):

Senthilvelan Santhakumar ◽

Jospaul Lukas ◽

Gopikrishnan Unnikrishnan ◽

Bejoy Thomas ◽

Chandrasekharan Kesavadas

Keyword(s):

Differential Diagnosis ◽

Liver Cirrhosis ◽

Genetic Mutation ◽

Imaging Findings ◽

Typical Appearance ◽

Manganese Transport

AbstractHypermanganesemia with dystonia and polycythemia along with liver cirrhosis is a rare syndromic complex that is associated with a characteristic genetic mutation and a typical appearance in the T1-weighted noncontrast image. We report the neuroimaging findings of two siblings affected by this syndrome. There are few reported cases in literature with similar findings. Diagnosing this problem will help in improving the outcomes as the condition is treatable. We have reviewed the clinical and imaging findings of this condition and the differential diagnosis related to it.

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Putative proteins involved in manganese transport across the blood-brain barr 1ier

Human & Experimental Toxicology ◽

10.1177/0960327107070496 ◽

2007 ◽

Vol 26 (4) ◽

pp. 295-302 ◽

Cited By ~ 22

Author(s):

Vanessa A Fitsanakis ◽

Greg Piccola ◽

Ana Paula Marreilha dos Santos ◽

Judy L Aschner ◽

Michael Aschner

Keyword(s):

In Vitro Models ◽

Complex Nature ◽

Divalent Metal Transporter 1 ◽

Divalent Metal Transporter ◽

Culture Techniques ◽

Metal Transporter ◽

Blood Brain ◽

Essential Nutrient ◽

Manganese Transport

Manganese (Mn) is an essential nutrient required for proper growth and maintenance of numerous biological systems. At high levels it is known to be neurotoxic. While focused research concerning the transport of Mn across the blood-brain barrier (BBB) is on-going, the exact identity of the transporter(s) responsible is still debated. The transferrin receptor (TfR) and the divalent metal transporter-1 (DMT-1) have long been thought to play a role in brain Mn deposition. However, evidence suggests that Mn may also be transported by other proteins. One model system of the BBB, rat brain endothelial (RBE4) cells, are known to express many proteins suspected to be involved in metal transport. This review will discuss the biological importance of Mn, and then briefly describe several proteins that may be involved in transport of this metal across the BBB. The latter section will examine the potential usefulness of RBE4 cells in characterizing various aspects of Mn transport, and basic culture techniques involved in working with these cells. It is hoped that ideas put forth in this article will stimulate further investigations into the complex nature of Mn transport, and address the importance as well as the limitation of in vitro models in answering these questions. Human & Experimental Toxicology (2007) 26, 295-302

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