Molecular species-specific differences in composition of triacylglycerols of mouse adipose tissue and diet

The zona pellucida, an extracellular matrix surrounding mammalian oocytes, is composed of three or four glycoproteins. It is well known that the zona pellucida plays several critical roles during fertilization, but there is little knowledge about its formation. The purpose of this study is to examine whether a pig zona pellucida glycoprotein 2 (pZP2) would assemble with mouse zona pellucida. A transgene construct was prepared by placing a minigene encoding pZP2 downstream from the promoter of mouse ZP2. The result showed that the transgenic protein was synthesized in growing oocytes but not incorporated into the zona pellucida. Furthermore, the pZP2 transgene did not rescue the phenotype in ZP2-knockout zona-deficient mice. These results indicate that pZP2 does not participate in mouse zona pellucida formation and the zona pellucida is constituted from its component proteins in a molecular species-specific manner between mice and pigs.

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The Composition of the Major Molecular Species of Adipose Tissue Triacylglycerols of Rats Reflects Those of Dietary Rapeseed, Olive and Sunflower Oils

Journal of Nutrition ◽

10.1093/jn/132.4.726 ◽

2002 ◽

Vol 132 (4) ◽

pp. 726-732 ◽

Cited By ~ 13

Author(s):

Nikolaus Weber ◽

Erika Klein ◽

Kumar D. Mukherjee

Keyword(s):

Adipose Tissue ◽

Molecular Species ◽

Sunflower Oils ◽

Major Molecular Species

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Immunophenotypical characterization of canine mesenchymal stem cells from perivisceral and subcutaneous adipose tissue by a species-specific panel of antibodies

Research in Veterinary Science ◽

10.1016/j.rvsc.2017.02.019 ◽

2017 ◽

Vol 114 ◽

pp. 51-58 ◽

Cited By ~ 6

Author(s):

Ana Ivanovska ◽

Stefano Grolli ◽

Paolo Borghetti ◽

Francesca Ravanetti ◽

Virna Conti ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Subcutaneous Adipose Tissue ◽

Species Specific ◽

Subcutaneous Adipose

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Effect of dietary linoleic acid content on the distribution of triacylglycerol molecular species in rat adipose tissue

Lipids ◽

10.1007/bf02536030 ◽

1992 ◽

Vol 27 (9) ◽

pp. 711-715 ◽

Cited By ~ 11

Author(s):

Yung-Sheng Huang ◽

X. Lin ◽

R. S. Smith ◽

P. R. Redden ◽

D. K. Jenkins ◽

...

Keyword(s):

Adipose Tissue ◽

Linoleic Acid ◽

Molecular Species ◽

Acid Content ◽

Linoleic Acid Content ◽

Dietary Linoleic Acid ◽

Triacylglycerol Molecular Species ◽

Rat Adipose Tissue

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Mechanisms of hepatic phosphatidylcholine synthesis in the developing guinea pig: contributions of acyl remodelling and of N-methylation of phosphatidylethanolamine

Biochemical Journal ◽

10.1042/bj2900067 ◽

1993 ◽

Vol 290 (1) ◽

pp. 67-73 ◽

Cited By ~ 25

Author(s):

G C Burdge ◽

F J Kelly ◽

A D Postle

Keyword(s):

Fatty Acids ◽

Guinea Pig ◽

Molecular Species ◽

Essential Fatty Acids ◽

Specific Radioactivity ◽

Long Chain Fatty Acids ◽

Radioactivity Analysis ◽

Species Specific ◽

Phosphatidylcholine Synthesis

Hepatic phosphatidylcholine (PC) from the immature fetal guinea pig at day 55 of gestation comprised mainly unsaturated molecular species containing C18:2(n-6) and C22:6(n-3) at the sn-2 position, reflecting placental permeability to essential fatty acids. At both day 55 and term (day 68), [Me-14C]choline was incorporated in utero over 3 h largely into sn-1-C16:0 PC species, with incorporation into sn-1-C18:0 PC species increasing by 18 h of incubation. Comparison of specific radioactivities after 3 h and 18 h suggests PC acyl remodelling by phospholipase A1. No incorporation into C20:4(n-6)-containing PC species could be detected of either [Me-14C]choline in vivo or CDP-[Me-14C]choline in isolated microsomes. The major phosphatidylethanolamine (PE) species were 16:0/22:6 and 18:0/22:6. Although [14C]ethanolamine was initially incorporated mainly into sn-1-C16:0 species, specific-radioactivity analysis suggested differential turnover rather than acyl remodelling. [1,2-14C]Ethanolamine and [Me-14C]methionine incorporation into PC molecular species indicated that both newly synthesized and total PE pools were available for N-methylation. Since the PC pool synthesized from PE included C20:4- and C22:6-containing species, N-methylation may provide a mechanism for supplying essential long-chain fatty acids to developing tissues that can be regulated independently from bulk PC synthesis.

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Influence of different dietary fats on triacylglycerol deposition in rat adipose tissue

British Journal Of Nutrition ◽

10.1017/s0007114500002130 ◽

2000 ◽

Vol 84 (5) ◽

pp. 756-774 ◽

Cited By ~ 19

Author(s):

Javier S. Perona ◽

María P. Portillo ◽

M. Teresa Macarulla ◽

Ana I. Tueros ◽

Valentina Ruiz-Gutiérrez

Keyword(s):

Adipose Tissue ◽

Fatty Acid ◽

Molecular Species ◽

Dietary Fat ◽

Dietary Fats ◽

Adipose Tissues ◽

Fa Composition ◽

Positional Isomerism ◽

Rat Adipose Tissue ◽

Subcutaneous Adipose

It has been demonstrated that triacylglycerol (TAG) mobilization from adipose tissue is selective and depends on fatty acid (FA) chain length, unsaturation and positional isomerism. The present study was performed to determine the influence of dietary fat on the composition of TAG stored in rat perirenal and subcutaneous adipose tissues. These results may provide information on the susceptibility of stored TAG to hydrolysis and further mobilization, and may help to establish an interrelationship between dietary composition and the FA efflux from adipose tissue. TAG molecular species and FA composition were determined by HPLC and GLC respectively. No significant differences were found in either FA or TAG composition between perirenal and subcutaneous adipose depots. The major FA in the dietary fats were present in the adipose tissues of the animals; in most cases, in similar proportions. However, differences were found between dietary and adipose tissue content of minor FA, which suggests that dietary FA composition is altered between ingestion and deposition in adipose tissue. The TAG molecular species of rat adipose tissue were enriched with the FA characteristic of each dietary fat. Dietary sunflower oil was responsible for enrichment with the most polar TAG. This finding may suggest easier mobilization of stored TAG. In conclusion, the process of fatty acid and TAG deposition in rat adipose tissue is selective, and depends on the composition of the diet.

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A NEUROGENIC GENE EXPRESSION SIGNATURE SUPPORTS HUMAN THERMOGENIC ADIPOSE TISSUE DEVELOPMENT IN VIVO.

10.1101/2021.12.29.474474 ◽

2021 ◽

Author(s):

Javier Solivan-Rivera ◽

Zinger Yang Loureiro ◽

Tiffany DeSouza ◽

Anand Desai ◽

Qin Yang ◽

...

Keyword(s):

Adipose Tissue ◽

Progenitor Cells ◽

Gene Expression Signature ◽

Metabolic Effects ◽

Mesenchymal Progenitor Cells ◽

Thermogenic Adipocytes ◽

Mouse Adipocytes ◽

Species Specific

Human beige/brite thermogenic adipose tissue exerts beneficial metabolic effects and may be harnessed to improve metabolic health. To uncover mechanisms by which thermogenic adipose tissue is generated and maintained we developed a species-hybrid model in which human mesenchymal progenitor cells are induced in vitro to differentiate into white or thermogenic adipocytes and are then implanted into immuno-compromised mice. Upon implantation, thermogenic adipocytes form a more densely vascularized and innervated adipose tissue compared to non-thermogenic adipocytes. Mouse endothelial and stem/progenitor cells recruited by implanted human thermogenic adipocytes are also qualitatively different, with differentially expressed genes mapping predominantly to circadian rhythm pathways. We trace the formation of this enhanced neurovascular architecture to higher expression of a distinct set of genes directly associated with neurogenesis (THBS4, TNC, NTRK3 and SPARCL1), and to lower expression of genes associated with neurotransmitter degradation (MAOA, ACHE) by adipocytes in the developed tissue. Further analysis reveals that MAOA is abundant in human adipocytes but absent in mouse adipocytes, revealing species-specific mechanisms of neurotransmitter tone regulation. In summary, our work discovers specific neurogenic genes associated with development and maintenance of human thermogenic adipose tissue, reveals species-specific mechanisms of control of neurotransmitter tone, and suggests that targeting adipocyte MAOA may be a strategy for enhancing thermogenic adipose tissue activity in humans.

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