The Composition of the Major Molecular Species of Adipose Tissue Triacylglycerols of Rats Reflects Those of Dietary Rapeseed, Olive and Sunflower Oils

This study attempted the lipid extraction process from the seeds of Madhuca ellitica, a lipid-rich plant, and conducted a lipidomic analysis on molecular species of the obtained product. Total lipids of the crude seeds were found to contain 11.2% of polar lipids. The major fatty acids (FAs) of the polar lipids were palmitic (16:0), stearic (18:0), oleic (18:1n-9), and linoleic (18:2n-6) acids, which amounted to 28.5, 12.5, 44.8, and 13.2% of total FAs, respectively. The content and chemical structures of individual molecular species of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidic acid (PA), and sulfoquinovosyldiacylglycerol (SQDG) were determined by HPLC with a tandem high-resolution mass spectrometry (HRMS). The major molecular species were 18:1/18:2 PE, 16:0/18:1 PC, 18:1/18:2 PC, 16:0/18:2 PG, 16:0/18:1 PG, 16:1/18:1 PI, 16:0/18:1 PI, 18:0/18:2 PI, 16:0/18:1 PA, 18:1/18:2 PA, 16:0/18:1 SQDG, and 18:0/18:1 SQDG. The application of a tandem HRMS allows us to determine the content of each isomer in pairs of the monoisotopic molecular species, for example, 18:0/18:2 and 18:1/18:1. The evaluation of the seed polar lipid profile will be helpful for developing the potential of this tree for nutritive and industrial uses.

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Simultaneous Determination of Phospholipid Classes and the Major Molecular Species of Lecithin in Human Amniotic Fluid by HPLC

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826079408013381 ◽

1994 ◽

Vol 17 (5) ◽

pp. 981-998 ◽

Cited By ~ 7

Author(s):

B. H. Klein ◽

J. W. Dudenhausen

Keyword(s):

Amniotic Fluid ◽

Simultaneous Determination ◽

Molecular Species ◽

Human Amniotic Fluid ◽

Phospholipid Classes ◽

Major Molecular Species

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Influence of different dietary fats on triacylglycerol deposition in rat adipose tissue

British Journal Of Nutrition ◽

10.1017/s0007114500002130 ◽

2000 ◽

Vol 84 (5) ◽

pp. 756-774 ◽

Cited By ~ 19

Author(s):

Javier S. Perona ◽

María P. Portillo ◽

M. Teresa Macarulla ◽

Ana I. Tueros ◽

Valentina Ruiz-Gutiérrez

Keyword(s):

Adipose Tissue ◽

Fatty Acid ◽

Molecular Species ◽

Dietary Fat ◽

Dietary Fats ◽

Adipose Tissues ◽

Fa Composition ◽

Positional Isomerism ◽

Rat Adipose Tissue ◽

Subcutaneous Adipose

It has been demonstrated that triacylglycerol (TAG) mobilization from adipose tissue is selective and depends on fatty acid (FA) chain length, unsaturation and positional isomerism. The present study was performed to determine the influence of dietary fat on the composition of TAG stored in rat perirenal and subcutaneous adipose tissues. These results may provide information on the susceptibility of stored TAG to hydrolysis and further mobilization, and may help to establish an interrelationship between dietary composition and the FA efflux from adipose tissue. TAG molecular species and FA composition were determined by HPLC and GLC respectively. No significant differences were found in either FA or TAG composition between perirenal and subcutaneous adipose depots. The major FA in the dietary fats were present in the adipose tissues of the animals; in most cases, in similar proportions. However, differences were found between dietary and adipose tissue content of minor FA, which suggests that dietary FA composition is altered between ingestion and deposition in adipose tissue. The TAG molecular species of rat adipose tissue were enriched with the FA characteristic of each dietary fat. Dietary sunflower oil was responsible for enrichment with the most polar TAG. This finding may suggest easier mobilization of stored TAG. In conclusion, the process of fatty acid and TAG deposition in rat adipose tissue is selective, and depends on the composition of the diet.

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Molecular species-specific differences in composition of triacylglycerols of mouse adipose tissue and diet

Nutrition Research ◽

10.1016/j.nutres.2008.02.007 ◽

2008 ◽

Vol 28 (4) ◽

pp. 258-262 ◽

Cited By ~ 4

Author(s):

Sayoko Suzuki ◽

Shin-ichi Ishikawa ◽

Keizou Arihara ◽

Makoto Itoh

Keyword(s):

Adipose Tissue ◽

Molecular Species ◽

Species Specific

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Virgin Olive Oil Normalizes the Altered Triacylglycerol Molecular Species Composition of Adipose Tissue in Spontaneously Hypertensive Rats

Journal of Agricultural and Food Chemistry ◽

10.1021/jf0498923 ◽

2004 ◽

Vol 52 (13) ◽

pp. 4227-4233 ◽

Cited By ~ 5

Author(s):

Javier S. Perona ◽

Valentina Ruiz-Gutierrez

Keyword(s):

Adipose Tissue ◽

Species Composition ◽

Olive Oil ◽

Molecular Species ◽

Spontaneously Hypertensive Rats ◽

Virgin Olive Oil ◽

Hypertensive Rats ◽

Molecular Species Composition ◽

Spontaneously Hypertensive ◽

Triacylglycerol Molecular Species

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Galactomannoproteins of Aspergillus fumigatus

Eukaryotic Cell ◽

10.1128/ec.4.7.1308-1316.2005 ◽

2005 ◽

Vol 4 (7) ◽

pp. 1308-1316 ◽

Cited By ~ 77

Author(s):

W. Morelle ◽

M. Bernard ◽

J.-P. Debeaupuis ◽

M. Buitrago ◽

M. Tabouret ◽

...

Keyword(s):

Monoclonal Antibody ◽

Aspergillus Fumigatus ◽

Phospholipase C ◽

Molecular Species ◽

Infected Patient ◽

Fungal Pathogens ◽

Chemical Characterization ◽

Major Molecular Species

ABSTRACT Galactofuranose-containing molecules have been repeatedly shown to be important antigens among human fungal pathogens, including Aspergillus fumigatus. Immunogenic galactofuran determinants have been poorly characterized chemically, however. We reported here the characterization of two glycoproteins of A. fumigatus with an N-glycan containing galactofuranose. These proteins are a phospholipase C and a phytase. Chemical characterization of the N-glycan indicates that it is a mixture of Hex5-13HexNAc2 oligosaccharides, the major molecular species corresponding to Hex6-8HexNAc2. The N-glycan contained one galactofuranose unit that was in a terminal nonreducing position attached to the 2 position of Man. This single terminal nonreducing galactofuranose is essential for the immunoreactivity of the N-glycans assessed either with a monoclonal antibody that recognizes a tetra-β-1,5-galactofuran chain of galactomannan or with Aspergillus-infected patient sera.

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