scholarly journals Tumor infiltrating lymphocytes (TIL) and prognosis in oral cavity squamous carcinoma: A preliminary study

Oral Oncology ◽  
2015 ◽  
Vol 51 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Gregory T. Wolf ◽  
Douglas B. Chepeha ◽  
Emily Bellile ◽  
Ariane Nguyen ◽  
Daffyd Thomas ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Squamous Carcinoma ◽  
Tumor Infiltrating Lymphocytes ◽  
Preliminary Study ◽  
Infiltrating Lymphocytes

Multiparameter study of the immunophenotype of tumor infiltrating lymphocytes, in cancer patients

2016 ◽  
Vol 21 (1-2) ◽  
pp. 51-54 ◽  
Author(s):  
Tat’yana N. Zabotina ◽  
O. V Korotkova ◽  
A. A Borunova ◽  
D. V Tabakov ◽  
I. I Bokin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Solid Tumors ◽  
Squamous Carcinoma ◽  
Tumor Infiltrating Lymphocytes ◽  
Tissue Level ◽  
Main Role ◽  
Regulatory T Lymphocytes ◽  
The Difference ◽  
Infiltrating Lymphocytes ◽  
First Time

For the first time in Russia the immunophenotype of tumor infiltrated lymphocytes (TIL) was investigated with the use of a method of quantitative flow cytometry. Samples from 104 patients, suffering from solid tumors (such as breast cancer, ovarian cancer, melanoma and oral cavity squamous carcinoma), were analyzed in this investigation. In the difference of cytomorphological analysis, the method of flow cytometry identified TIL in 100% of cases. In the structure of CDS+Т- lymphocytes the ratio CD4/CD8 was equal to 1,1±0,1. Serial gating strategy allowed to assess minor subpopulations of regulatory T-lymphocytes CD45+CD4+CD127+low/neg and CD45+CD8+CD28-CD11b-. Regulatory mechanisms with involvement of CD8 T-cells played the main role in the generation of immune responses at the tissue level ofpatients, suffering from solid tumors, independent of nosological form of disease.

Associations Between ADC Texture Analysis and Tumor Infiltrating Lymphocytes in Brain Metastasis – A Preliminary Study

2021 ◽  
Vol 41 (9) ◽  
pp. 4549-4554
Author(s):  
HANS-JONAS MEYER ◽  
ANNE-KATHRIN HOHN ◽  
GORDIAN PRASSE ◽  
KARL-TITUS HOFFMANN ◽  
ALEXEY SUROV
Keyword(s):  
Brain Metastasis ◽  
Texture Analysis ◽  
Tumor Infiltrating Lymphocytes ◽  
Preliminary Study ◽  
Infiltrating Lymphocytes

scholarly journals The Concentration of CMKLR1 Expression on Clinicopathological Parameters of Colorectal Cancer: A Preliminary Study

Medicina ◽  
2021 ◽  
Vol 57 (12) ◽  
pp. 1299
Author(s):  
Paweł Kiczmer ◽  
Sylwia Mielcarska ◽  
Magdalena Chrabańska ◽  
Miriam Dawidowicz ◽  
Agnieszka Kula ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Tumor Infiltrating Lymphocytes ◽  
Lymphocytic Infiltration ◽  
Clinicopathological Parameters ◽  
Cancer Growth ◽  
Enzyme Linked Immunosorbent Assay ◽  
Common Cause ◽  
Biochemical Pathways ◽  
Preliminary Study ◽  
Infiltrating Lymphocytes

Background and Objectives: Colorectal cancer (CRC) is the second-most common cause of cancer-related deaths worldwide. Angiogenesis is crucial for cancer growth, infiltration of surrounding tissues, and metastasis and plays a key role in the pathogenesis of CRC. Chemerin/chemokine-like receptor 1 (CMKLR1) is one of the biochemical pathways involved in the regulation of angiogenesis in solid tumors. The aim of the study was to assess the CMKLR1 level in tumor and margin tissues of CRC in relation to histopathological parameters: microvessel density (MVD), budding, tumor-infiltrating lymphocytes (TILs), TNM scale, and grading. Materials and Methods: The study involved 43 samples of tumor and margin tissues obtained from CRC patients. To assess the concentration of CMKLR1 a commercially available enzyme-linked immunosorbent assay kit was used. For 35 cases, we performed CD34 immunostaining. The MVD, budding, and TILs were assessed using a light microscope. Results: The levels of CMKLR1 in both tumor and margin were negatively correlated with MVD and budding. CMKLR1 concentration in margin was higher in tissues with lymphocytic infiltration. Conclusions: Low vascularity and low budding are associated with higher CMKLR1 expression. CMKLR1 might play a multifunctional role in CRC pathogenesis by influencing tumor budding and peritumoral lymphocytic infiltration.

scholarly journals Modeling ex vivo tumor-infiltrating lymphocyte expansion from established solid malignancies

2021 ◽  
Author(s):  
HM Knochelmann ◽  
AM Rivera-Reyes ◽  
MM Wyatt ◽  
AS Smith ◽  
R Chamness ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Oral Cavity Cancer ◽  
Ex Vivo ◽  
Tumor Infiltrating Lymphocytes ◽  
Tumor Escape ◽  
Multiple Tumor ◽  
Mouse Tumors ◽  
Cancer Models ◽  
Solid Malignancies ◽  
Infiltrating Lymphocytes

AbstractAdoptive transfer of tumor-infiltrating lymphocytes (TIL) elicits the regression of metastatic malignancies, yet a low proportion of patients achieve complete durable responses. The high incidence of relapse in these patients highlights the need to better understand mechanisms of tumor escape from T cell control. While melanoma has provided the foundation for developing TIL therapy, much less is known about TIL efficacy and relapse in other malignancies. We sought to investigate TIL characteristics in mouse tumors which have not been studied in this setting. Here, we expanded murine TIL ex vivo in IL-2 from fragments of multiple tumor models, including oral cavity cancer models of varying immunogenicity. Additionally, TIL was expanded from pmel-1 mice bearing B16 melanoma, yielding an enriched population of tumor-infiltrating TCR transgenic T cells. Murine TILs are similar to human TIL in that they express high levels of inhibitory receptors (PD-1, Tim-3, etc.) and can be expanded ex vivo in IL-2 extensively. Of clinical relevance, we draw parallels between murine and patient TIL, evaluating relationships between PD-1, Lag-3, and Tim-3 on TILs from a cohort of oral cavity cancer patients. This platform can be used by labs even in the absence of clinical specimens or clean cell facilities and will be important to more broadly understand TIL phenotypes across many different malignancies.

Immune signatures associated with response to neoadjuvant PD-1 blockade in oral cavity cancer.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. 6055-6055
Author(s):  
Hannah Knochelmann ◽  
Joshua Dean Horton ◽  
Megan Meek ◽  
Carsten Krieg ◽  
Cynthia Dawn Timmers ◽  
...  
Keyword(s):  
T Cells ◽  
Oral Cavity ◽  
Clinical Medicine ◽  
Ex Vivo ◽  
Tumor Infiltrating Lymphocytes ◽  
Effector Memory ◽  
Immune Profile ◽  
Phenotypic Differences ◽  
Small Cohort ◽  
Infiltrating Lymphocytes

6055 Background: PD-1 inhibition therapy has revolutionized clinical medicine as it can mediate durable responses in a small cohort of patients. Yet, it remains incompletely understood why these patients respond. To address this question, we studied patients with oral cavity squamous cell carcinoma (OCSCC) to elucidate immune phenotypes associated with response to nivolumab. Methods: We defined the immune profile from the blood and tumor of patients on neoadjuvant nivolumab. We tested if tumor-infiltrating lymphocytes (TIL) could be preferentially expanded ex vivo from nivolumab-responsive patients versus those who were either non-responsive or had never received nivolumab. During the course of therapy, we comprehensively profiled a number of surface markers on patients’ T cells to define their activation status, cytotoxic capacity and memory phenotype. Moreover, the immune profile of the peripheral blood was assessed pre- and post-nivolumab using high dimensional mass cytometry. Results: Regardless of PD-1 therapy, TIL were successfully expanded from 11 of the 12 patients. TIL were comprised of both CD4+ and CD8+ T cells. Additional investigation revealed that the frequency of CD4+ T cells and effector memory T cells in TIL correlated with disease progression (CD4: p = 0.04, r = 0.74, effector memory: p = 0.046, r = 0.74). TILs from responders expressed higher CD26 (p = 0.007, r = -0.88) and Tim3 (p = 0.045, r = -0.74) while PD-1, Lag3, and Ox40 were not differentially expressed based on response. Spearman correlation and Mann Whitney U test were used to assess phenotypic differences. Conclusions: We demonstrate, for the first time, that TIL can be reliably expanded from OCSCC patients on neoadjuvant nivolumab. Moreover, individuals who were responsive to PD-1 blockade had TIL expressing high levels of CD26 and Tim3. Future studies will explore if these markers are predictive of responses and if they contribute to treatment outcome.

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