Tumor-selective near-infrared photodynamic therapy with novel indocyanine green-loaded nanocarrier delays paralysis in rats with spinal metastasis

2013 ◽  
Vol 10 (4) ◽  
pp. 374-378 ◽  
Author(s):  
Toru Funayama ◽  
Toshinori Tsukanishi ◽  
Isao Hara ◽  
Eiichi Ozeki ◽  
Masataka Sakane
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Near Infrared ◽  
Spinal Metastasis ◽  
Tumor Selective

scholarly journals Broad spectrum antibacterial photodynamic and photothermal therapy achieved with indocyanine green loaded SPIONs under near infrared irradiation

2020 ◽  
Vol 8 (16) ◽  
pp. 4616-4625 ◽  
Author(s):  
K. Bilici ◽  
N. Atac ◽  
A. Muti ◽  
I. Baylam ◽  
O. Dogan ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Broad Spectrum ◽  
Photothermal Therapy ◽  
Bacterial Infections ◽  
Near Infrared ◽  
Alternative Therapies ◽  
Antimicrobial Photodynamic Therapy ◽  
Infrared Irradiation ◽  
Antibiotic Resistant

Antimicrobial photodynamic therapy (aPDT) and antimicrobial photothermal therapy (aPTT) are promising local and effective alternative therapies for antibiotic resistant bacterial infections and biofilms.

scholarly journals Intraoperative Near-infrared Fluorescence Imaging with Novel Indocyanine Green-Loaded Nanocarrier for Spinal Metastasis: A Preliminary Animal Study

2012 ◽  
Vol 6 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Toru Funayama ◽  
Masataka Sakane ◽  
Tetsuya Abe ◽  
Isao Hara ◽  
Eiichi Ozeki ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Fluorescence Imaging ◽  
Rat Model ◽  
Near Infrared ◽  
Imaging Modality ◽  
Intraoperative Imaging ◽  
Spinal Metastasis ◽  
Vena Cava ◽  
Nir Fluorescence

Marginal resection during resection of a spinal metastasis is frequently difficult because of the presence of important tissues such as the aorta, vena cava, and dura mater, including the spinal cord adjacent to the vertebral body. Thus, there is an urgent need for novel intraoperative imaging modalities with the ability to clearly identify bone metastasis. We have proposed a novel nanocarrier loaded with indocyanine green (ICG) (ICG-lactosome) with tumor selectivity attributable to its enhanced permeation and retention (EPR) effect. We studied its feasibility in intraoperative near-infrared (NIR) fluorescence diagnosis with ICG-lactosome for imaging spinal metastasis. A rat model of subcutaneous mammary tumor and a rat model of spinal metastasis of breast cancer were used. Fluorescence emitted by the subcutaneous tumors and the spinal metastasis were clearly detected for at least 24 h. Moreover, imaging of the dissected spine revealed clear fluorescence emitted by the metastatic lesion in the L6 vertebra while the normal bone lacked fluorescence. This study was the first report on NIR fluorescence imaging of spinal metastasis in vivo. NIR fluorescence imaging with ICG-lactosome could be an effective intraoperative imaging modality for detecting spinal metastasis.

In-vitro efficacy of indocyanine green-mediated photodynamic therapy in combination with cisplatin or etoposide

2015 ◽  
Vol 4 (4) ◽  
Author(s):  
Kamola Kasimova ◽  
Lothar Lilge ◽  
Brian C. Wilson
Keyword(s):  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Tumor Cells ◽  
Near Infrared ◽  
Lung Tumor ◽  
Infrared Light ◽  
Cancer Therapies ◽  
Cytotoxic Effects ◽  
Limited Efficacy

Abstract:Localizing the cytotoxic effects of cancer therapies to only affect the tumor cells is a goal in oncology, to maximize efficacy and minimize treatment-related morbidities. Most effective chemotherapeutic drugs have significant side effects due to off-target toxicity. By comparison, photodynamic therapy (PDT) is a localized therapy without significant systemic toxicity but may have limited efficacy. Hence, combining PDT with chemotherapy was investigated to determine if the anti-tumor effect of the latter could be enhanced. PDT using indocyanine green (ICG), activated by near-infrared light, was investigated in lung tumor cells

scholarly journals Indocyanine Green—Mediated Photodynamic Therapy Reduces Methicillin-Resistant Staphylococcus aureus Drug Resistance

2019 ◽  
Vol 8 (3) ◽  
pp. 411 ◽  
Author(s):  
Tak-Wah Wong ◽  
Shu-Zhen Liao ◽  
Wen-Chien Ko ◽  
Chi-Jung Wu ◽  
Shin Wu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Photodynamic Therapy ◽  
Indocyanine Green ◽  
Drug Sensitivity ◽  
Near Infrared ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Mouse Lung ◽  
Wound Infections ◽  
Methicillin Resistant

Methicillin-resistant Staphylococcus aureus (MRSA) skin-wound infections are associated with considerable morbidity and mortality. Indocyanine green (ICG), a safe and inexpensive dye used in clinical imaging, can be activated by near-infrared in photodynamic therapy (PDT) and photothermal therapy (PTT) to effectively kill MRSA. However, how this treatment affects MRSA drug sensitivity remains unknown. The drug-sensitivity phenotypes, bacterial growth rate, and cell-wall thickness of three MRSA strains were analyzed after ICG-PDT. Drug-resistant gene expressions were determined by polymerase chain reaction (PCR) and quantitative reverse transcription (qRT)-PCR. Related protein expressions were examined with immunoblotting. Drug sensitivity was further evaluated in animal models. MRSA that survived the treatment grew faster, and the cell wall became thinner compared to parental cells. These cells became more sensitive to oxacillin, which was partly related to mecA complex gene deletion. Skin necrosis caused by ICG-PDT-treated MRSA infection was smaller and healed faster than that infected with parental cells. With oxacillin therapy, no bacteria could be isolated from mouse lung tissue infected with ICG-PDT-treated MRSA. ICG-PDT drives MRSA toward an oxacillin-sensitive phenotype. It has the potential to develop into an alternative or adjuvant clinical treatment against MRSA wound infections.

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