In order to identify possible mislabeling of the apple accession maintained
ex situ in Srebrenik and to gain insight into the genetic structure of the
conserved germplasm, 14 accessions from the collection were genotyped using
10 SSR (Simple Sequence Repeats) markers. Obtained SSR profiles were then
added to an existing database constructed for previously characterized 24
traditional and 13 international, reference apple cultivars maintained at the
same collection. Bayesian analysis implemented in the STRUCTURE program
grouped 42 out of 51 analyzed apple accessions (38 traditional and 13
international) into three RPPs (reconstructed panmictic populations) with
probability of membership qI higher than 75%. Almost all international,
reference cultivars grouped in RPP3, whereas traditional B&H cultivars from
the Srebrenik collection grouped in all three RPPs. Large and significant
differentiations between all three individual RPPs were detected through the
analyses of molecular variance and confirmed with FCA (factorial
correspondence analyses). NJ cluster analysis, based on the Bruvo genetic
distance, revealed that out of 38 traditional B&H apple cultivars, analyzed
in the study, ?Ljepocvjetka?, ?Bobovec? and ?Bobovec J? grouped closest to
the international reference cultivars. Available date indicates that unlike a
large number of B&H apple cultivars which were introduced during the reign of
the Ottoman Empire, ?Ljepocvjetka? and ?Bobovec? were probably introduced at
a later date. Cluster analyses also enabled the detection of one synonym and
three homonyms within the collection. In four cases, previously conducted
identification based on phenotypic analyses was confirmed by genetic
analyses. Results of the structure analyses indicate a heterogeneous genetic
structure of the analyzed accessions. This characteristic of the B&H apple
germplasm could be useful for future breeding programs.