Antiproliferative quillaic acid and gypsogenin saponins from Saponaria officinalis L. roots

2015 ◽  
Vol 113 ◽  
pp. 108-120 ◽  
Author(s):  
Yuping Lu ◽  
Dang Van ◽  
Leah Deibert ◽  
Greg Bishop ◽  
John Balsevich
Pharmacia ◽  
2021 ◽  
Vol 68 (2) ◽  
pp. 339-345
Author(s):  
Liudmyla Slobodianiuk ◽  
Liliia Budniak ◽  
Svitlana Marchyshyn ◽  
Liliya Kostyshyn ◽  
Oleksandr Zakharchuk

Saponaria officinalis L. (common soapwort), usually named fuller’s herb, is encountered in most of Europe, in Spain, France, Italy, for example, and also in Syria and North Africa. Saponaria officinalis L. is known in most of the world as an introduced species, often a weed, and sometimes as cultivated decorative plant. Saponaria officinalis contains a large amount of saponins, which foam during extraction with water. In addition to saponins, common soapwort also contains flavonoids, quillaic acid, fatty acids and different phenolic compounds. There is a lack of information about carbohydrates content of common soapwort. Thus the aim of this study was to determine the content of carbohydrates Saponaria officinalis L. herb and roots. The qualitative composition and quantitative content of carbohydrates in herb and roots of Saponaria officinalis L. were determined by using GC/MS method. The studies have shown that Saponaria officinalis L. herb is mainly composed of free carbohydrates such as D-glucose (3.65 mg/g), D-galactose (0.29 mg/g), D-fructose (0.20 mg/g) and D-saccharose (3.72 mg/g). In common soapwort herb, after acidic hydrolysis and derivatization with acetylated aldononitriles, D-arabinose, D-fucose, D-mannose, D-glucose, D-galactose, D-fructose and Myo-inositol were identified. Free carbohydrates in the roots of Saponaria officinalis L., including D-glucose, D-galactose and D-saccharose, were determined with GC/MS method too. D-saccharose was common among free carbohydrates of Saponaria officinalis L. in the largest amounts. Its content in herb and roots of the common soapwort was 3.72 mg/g and 25.39 mg/g respectively.


Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YA Jeon ◽  
HS Lee ◽  
ES Park ◽  
YY Lee ◽  
JS Sung ◽  
...  

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2072
Author(s):  
Maria Antonia Tănase ◽  
Maria Marinescu ◽  
Petruta Oancea ◽  
Adina Răducan ◽  
Catalin Ionut Mihaescu ◽  
...  

In the present work, the properties of ZnO nanoparticles obtained using an eco-friendly synthesis (biomediated methods in microwave irradiation) were studied. Saponaria officinalis extracts were used as both reducing and capping agents in the green nanochemistry synthesis of ZnO. Inorganic zinc oxide nanopowders were successfully prepared by a modified hydrothermal method and plant extract-mediated method. The influence of microwave irradiation was studied in both cases. The size, composition, crystallinity and morphology of inorganic nanoparticles (NPs) were investigated using dynamic light scattering (DLS), powder X-ray diffraction (XRD), SEM-EDX microscopy. Tunings of the nanochemistry reaction conditions (Zn precursor, structuring agent), ZnO NPs with various shapes were obtained, from quasi-spherical to flower-like. The optical properties and photocatalytic activity (degradation of methylene blue as model compound) were also investigated. ZnO nanopowders’ antibacterial activity was tested against Gram-positive and Gram-negative bacterial strains to evidence the influence of the vegetal extract-mediated synthesis on the biological activity.


2020 ◽  
pp. 193-212
Author(s):  
Marco R. Soria ◽  
Luca Benatti ◽  
Gianpaolo Nitti ◽  
Aldo Ceriotti ◽  
Michela Solinas ◽  
...  

Author(s):  
Mohamed Réda Sefrioui ◽  
Ibrahim Sbai El Othmani ◽  
Halima Filali ◽  
Sanae Derfoufi ◽  
Soufiane Derraji ◽  
...  

Background and objective. Chemical spermicides currently marketed and widely used are known to have many side effects. Thereby, and in order to look for more tolerated natural spermicidal agents, the aim of this work was to evaluate the spermicidal potential of saponin extracts from the roots of Saponaria officinalis / Caryophyllaceae, Glycyrrhizia glabra / Fabaceae, and Herniaria glabra / Caryophyllaceae by studying their in vitro effects on sperm mobility and vitality. Methods. Methanolic saponin extracts from the plants roots were performed. Sperm suspensions were prepared by centrifugation on a PureSperm® density gradient (70 and 45%) and incubated with various concentrations of saponin extracts (50, 250, 500 and 750 mg/mL) at 37°C. The spermicidal activity was evaluated by studying the mobility and vitality of spermatozoa at different time intervals ranging from 10 to 240 minutes. Results. A dose and time dependent effect on sperm mobility and vitality was observed for our extracts. Extracts from Saponaria officinalis roots induced an irreversible immobilization and a total non-viability of sperm within 10 minutes at a concentration of 750 mg/mL. A similar effect was observed within 30 minutes at 750 mg/mL for Herniaria glabra extract and within 90 minutes at 500 mg/ml for Glycyrrhizia glabra extract. Conclusion. The results of our study showed that the saponin extracts of our plants roots possess potent in vitro dose and time dependant spermicidal effect. These natural products could therefore represent a safer and better tolerated alternative to chemical spermicides.


1996 ◽  
Vol 319 (2) ◽  
pp. 507-513 ◽  
Author(s):  
Luigi BARBIERI ◽  
Paola VALBONESI ◽  
Paola GORINI ◽  
Annalisa PESSION ◽  
Fiorenzo STIRPE

The ribosome-inactivating proteins (RIPs) are a family of plant enzymes for which a unique activity has been determined: rRNA N-glycosidase, which removes adenine at a specific universally conserved position (A4324 in the case of rat ribosomes). Here we report that saporin-L1, a RIP from the leaves of Saponaria officinalis, recognizes other substrates, including RNAs from different sources, DNA and poly(A). Saporin-L1 depurinated DNA extensively and released adenine from all adenine-containing polynucleotides tested. Adenine was the only base released from DNA or artificial polynucleotides. The characteristics of the reactions catalysed by saporin-L1 have been determined: optimal pH and temperature, ionic requirements, and the kinetic parameters Km and kcat. The reaction proceeded without cofactors, at low ionic strength, in the absence of Mg2+ and K+. Saporin-L1 had no activity towards various adenine-containing non-polynucleotide compounds (cytokinins, cofactors, nucleotides). This plant protein may now be classified as a polynucleotide:adenosine glycosidase.


Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2527
Author(s):  
Monika Rojewska ◽  
Wojciech Smułek ◽  
Krystyna Prochaska ◽  
Ewa Kaczorek

Due to the increasing use of antibiotics, measures are being taken to improve their removal from the natural environment. The support of biodegradation with natural surfactants that increase the bioavailability of impurities for microorganisms that degrade them, raises questions about their effect on bacterial cells. In this paper we present analysis of the interaction of nitrofurantoin (NFT) and saponins from the Saponaria officinalis on the environmental bacteria membrane and the model phospholipid membrane mimicking it. A wide perspective of the process is provided with the Langmuir monolayer technique and membrane permeability test with bacteria. The obtained results showed that above critical micelle concentration (CMC), saponin molecules are incorporated into the POPE monolayer, but the NFT impact was ambiguous. What is more, differences in membrane permeability between the cells exposed to NFT in comparison to that of the non-exposed cells were observed above 1.0 CMC for Achromobacter sp. KW1 or above 0.5 CMC for Pseudomonas sp. MChB. In both cases, NFT presence lowered the membrane permeability. Moreover, the Congo red adhesion to the cell membrane also decreased in the presence of a high concentration of surfactants and NFT. The results suggest that saponins are incorporated into the bacteria membrane, but their sugar hydrophilic part remains outside, which modifies the adsorption properties of the cell surface as well as the membrane permeability.


Planta ◽  
1994 ◽  
Vol 194 (4) ◽  
pp. 461-470 ◽  
Author(s):  
Raffaella Carzaniga ◽  
Lesley Sinclair ◽  
Anthony P. Fordham-Skelton ◽  
Nick Harris ◽  
Ronald R. D. Croy

2006 ◽  
Vol 17 (6) ◽  
pp. 414-423 ◽  
Author(s):  
J. John Balsevich ◽  
Greg G. Bishop ◽  
Irving Ramirez-Erosa
Keyword(s):  

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