scholarly journals Porphyromonas gingivalis, a cause of preterm birth in mice, induces an inflammatory response in human amnion mesenchymal cells but not epithelial cells

Placenta ◽  
2020 ◽  
Vol 99 ◽  
pp. 21-26
Author(s):  
Haruhisa Konishi ◽  
Satoshi Urabe ◽  
Yuko Teraoka ◽  
Yoshito Morishita ◽  
Iemasa Koh ◽  
...  
Keyword(s):  
Preterm Birth ◽  
Epithelial Cells ◽  
Inflammatory Response ◽  
Porphyromonas Gingivalis ◽  
Mesenchymal Cells ◽  
Human Amnion

scholarly journals Effect of Human Amnion Epithelial Cells on the Acute Inflammatory Response in Fetal Sheep

2017 ◽  
Vol 8 ◽  
Author(s):  
Alana Westover ◽  
Jacqueline M. Melville ◽  
Courtney McDonald ◽  
Rebecca Lim ◽  
Graham Jenkin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Acute Inflammatory Response ◽  
Fetal Sheep ◽  
Human Amnion ◽  
Amnion Epithelial Cells

scholarly journals Human amnion epithelial cells modulate the inflammatory response to ventilation in preterm lambs

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173572 ◽  
Author(s):  
Jacqueline M. Melville ◽  
Courtney A. McDonald ◽  
Robert J. Bischof ◽  
Graeme R. Polglase ◽  
Rebecca Lim ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Human Amnion ◽  
Amnion Epithelial Cells

scholarly journals Keratinocyte Growth Factor Expression in the Mesenchymal Cells of Human Amnion*

1997 ◽  
Vol 82 (10) ◽  
pp. 3319-3323 ◽  
Author(s):  
M. Linette Casey ◽  
Paul C. MacDonald
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Transforming Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Transforming Growth Factor Β ◽  
Mesenchymal Cells ◽  
Cell Replication ◽  
Human Amnion ◽  
Tetradecanoyl Phorbol Acetate ◽  
Amnion Epithelial Cells

Abstract Amnion epithelial and mesenchymal cells were separated by differential protease treatment, and the separated cells were maintained in monolayer culture. Keratinocyte growth factor (KGF) messenger RNA (mRNA) was readily detected by Northern analysis of amnion mesenchymal cell total RNA (10 μg) but not in amnion epithelial cells. Treatment of the amnion mesenchymal cells in serum-free medium with tetradecanoyl phorbol acetate (1 nm) caused an increase in the level of KGF mRNA. Forskolin treatment also caused an increase in KGF mRNA but not to the levels attained with tetradecanoyl phorbol acetate treatment. Dexamethasone (1 nm) treatment of these cells effected a reduction in the level of KGF mRNA. Prolonged maintenance of mesenchymal cells in serum-free medium also was associated with an increase in the level of KGF mRNA. Treatment with a variety of other agents, viz., interleukin (IL)-1, IL-6 plus or minus IL-6 soluble receptor, IL-11, oncostatin M , epidermal growth factor (EGF), and transforming growth factor-β did not modify the level of KGF mRNA. Treatment of amnion epithelial cells with KGF caused an increase in the rate of [3H]thymidine incorporation, but the rate of cell replication induced by KGF was less than that induced by treatment with EGF. Transforming growth factor-β treatment inhibited basal and EGF- and KGF-stimulated amnion epithelial cell replication. The findings of this study are indicative that KGF is expressed in human amnion mesenchymal cells, and that KGF may act on the epithelial cells of this tissue.

scholarly journals Effect of Thrombin on Human Amnion Mesenchymal Cells, Mouse Fetal Membranes, and Preterm Birth

2014 ◽  
Vol 289 (19) ◽  
pp. 13295-13307 ◽  
Author(s):  
Haruta Mogami ◽  
Patrick W. Keller ◽  
Haolin Shi ◽  
R. Ann Word
Keyword(s):  
Preterm Birth ◽  
Mesenchymal Cells ◽  
Fetal Membranes ◽  
Human Amnion

scholarly journals Effect of Lipopolysaccharides (LPS) and Lipoteichoic acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2058
Author(s):  
Nicole Reisinger ◽  
Dominik Wendner ◽  
Nora Schauerhuber ◽  
Elisabeth Mayer
Keyword(s):  
Epithelial Cells ◽  
Inflammatory Response ◽  
Structural Integrity ◽  
Animal Health ◽  
Lipoteichoic Acid ◽  
Local Inflammatory Response ◽  
Tissue Integrity ◽  
Separation Force ◽  
The Impact ◽  
Rumen Epithelial Cells

Endotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (RECs) were stimulated with LPS and LTA for 1, 2, 4, and 24 h. Thereafter, the expression of selected genes of the LPS and LTA pathway and inflammatory response were evaluated. Furthermore, it was assessed whether LPS affects inflammatory response and structural integrity of claw explants. Therefore, claw explants were incubated with LPS for 4 h to assess the expression of selected genes and for 24 h to evaluate tissue integrity via separation force. LPS strongly affected the expression of genes related to inflammation (NFkB, TNF-α, IL1B, IL6, CXCL8, MMP9) in RECs. LTA induced a delayed and weaker inflammatory response than LPS. In claw explants, LPS affected tissue integrity, as there was a concentration-dependent decrease of separation force. Incubation time had a strong effect on inflammatory genes in claw explants. Our data suggest that endotoxins can induce a local inflammatory response in the rumen epithelium. Furthermore, translocation of LPS might negatively impact claw health.

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