A PCR-based diagnostic tool for distinguishing grape skin color mutants

Anthocyanins in red grape skin have a positive effect on fruit color and human health. The effect of foliar potassium application on anthocyanin accumulation in grape skin is not well understood. The study aimed to better understand the mechanism of anthocyanin accumulation in grape skin in response to foliar sprays of K2SO4 and KH2PO4. In this study, we investigated the effects of foliar application of KH2PO4 (T2), K2SO4 (T1) and distilled water (CK) on the skin color of ‘Kyoho’ grapes at mid-ripe and mature stages. At 90 and 110 days after full bloom (DAFB), T2 had the greatest total soluble solids (TSS), flavonoid and total anthocyanin contents, followed by T1 and CK. At two stages, the titratable acid content decreased and the juice pH increased under T2 treatment relative to CK. T1 and T2 had lower lightness (L*) than CK, and the color index of red grapes (CIRG) under T1 and T2 increased at two stages compared to CK. KEGG metabolic pathway analysis revealed that flavonoid biosynthesis was the most significantly enriched pathway in CK vs. T2 at 90 and 110 DAFB. At 90 DAFB, T2 had higher expressions of phenylalanine ammonia-lyas (PAL), cytochrome P450 CYP73A100 (CYP73A), 4-coumarate: CoA ligase (4CL), chalcone synthase (CHS), flavanone 3-dioxygenase-like (F3H) and UDP glucose: flavonoid 3-o-glucosyl transferase (UFGT) than CK and T1. Foliar application of potassium fertilizer may accelerate anthocyanin accumulation by altering the transcript levels of PAL, CYP73A, 4CL, CHS, F3H, and UFGT of the flavonoid biosynthesis.

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Retrotransposon-Induced Mutations in Grape Skin Color

Science ◽

10.1126/science.1095011 ◽

2004 ◽

Vol 304 (5673) ◽

pp. 982-982 ◽

Cited By ~ 498

Author(s):

S. Kobayashi

Keyword(s):

Skin Color ◽

Induced Mutations ◽

Grape Skin

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Harpin Proteins Improve Bioactive Compounds Content in Crimson Seedless Table Grape

The Open Bioactive Compounds Journal ◽

10.2174/1874847301907010001 ◽

2019 ◽

Vol 7 (1) ◽

pp. 1-7

Author(s):

Pasquale Crupi ◽

Giambattista Debiase ◽

Gianvito Masi ◽

Francesca Mangione ◽

Luigi Tarricone

Keyword(s):

Bioactive Compounds ◽

Skin Color ◽

Anthocyanin Biosynthesis ◽

Table Grape ◽

Berry Skin ◽

Grape Skin ◽

Growing Period ◽

Commercial Harvest ◽

Red Color ◽

And Control

Background: Frequently, in warm climates such as Mediterranean areas, a red-pink table grape ‘Crimson Seedless’ does not reach a good berry skin color; and an acceptable anthocyanin bioactive compounds content, responsible for the red color of berries. Harpin proteins are biotechnologically developed bio-activators that, if applied on plants during the growing period, trigger the expression of hundreds of genes among which those associated with the biosynthesis of bioactive compounds (such as anthocyanins). Aim: This research aimed at using harpin proteins to test their suitability in improving the grape skin color. Methods and Materials: Beta-harpin protein 1% p.a. (400 g/Ha) was applied to ‘Crimson Seedless’ vines three times at the beginning of veraison. Six samplings were carried out for both the treated and control grapes until commercial harvest. In the skin extracts, total and individual anthocyanins content was determined by UV-Vis and RP-HPLC-DAD analyses, respectively. Results: The collected results confirmed that the application of harpin proteins effectively stimulated the anthocyanin biosynthesis leading to make peonidin-3O-glucoside, cyanidin-3O-glucoside, and malvidin-3O-glucoside values from 2 to almost 10 folds higher in treated grapes than in control grapes (P < 0.05). Conclusion: Actually, harpin proteins improved the color of the berry skin, leading to a significantly higher concentration of anthocyanins in treated than in control grapes.

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Table Grapes: There Is More to Vitiviniculture than Wine…

10.5772/intechopen.99986 ◽

2021 ◽

Author(s):

Ana Cristina Agulheiro-Santos ◽

Marta Laranjo ◽

Sara Ricardo-Rodrigues

Keyword(s):

Skin Color ◽

Consumer Preferences ◽

Sugar Content ◽

Morphological Description ◽

Human Consumption ◽

Grape Skin ◽

Table Grapes ◽

Introductory Note ◽

The Impact ◽

World Distribution

Table grapes are fruits intended for fresh human consumption due to their sensory attributes and nutritional value. The objective of this chapter is to review the existing knowledge about table grapes, including a description of different varieties, with particular emphasis on the new highly appreciated seedless varieties. Following an introductory note on the world distribution and production of table grapes, also considering the impact of climate change, selected varieties of table grapes will be characterized in terms of their physiology, postharvest features, and consumer preferences. A morphological description of each variety, with emphasis on grape skin, grape rachis and grape cluster will be included. A final note on the drying of table grapes into raisins, and the most appropriate varieties for drying, will be given. The major changes occurring throughout the growth, development, and ripening phases of table grapes production will be discussed, regarding both physical (skin color and skin and pulp texture) and chemical (phenolic compounds, sugar content and acidity) parameters, as well as growth regulators.

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The Elicited Language Analysis Procedure

Language Speech and Hearing Services in Schools ◽

10.1044/0161-1461.1301.37 ◽

1982 ◽

Vol 13 (1) ◽

pp. 37-41

Author(s):

Larry J. Mattes

Keyword(s):

Diagnostic Tool ◽

Descriptive Analysis ◽

Quality Measures ◽

Analysis Procedure ◽

Elicited Imitation ◽

Response Quality ◽

Semantic Relationships ◽

Language Analysis

Elicited imitation tasks are frequently used as a diagnostic tool in evaluating children with communication handicaps. This article presents a scoring procedure that can be used to obtain an in-depth descriptive analysis of responses produced on elicited imitation tasks. The Elicited Language Analysis Procedure makes it possible to systematically evaluate responses in terms of both their syntactic and semantic relationships to the stimulus sentences presented by the examiner. Response quality measures are also included in the analysis procedure.

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