GsLRPK, a novel cold-activated leucine-rich repeat receptor-like protein kinase from Glycine soja, is a positive regulator to cold stress tolerance

Plant Science ◽  
2014 ◽  
Vol 215-216 ◽  
pp. 19-28 ◽  
Author(s):  
Liang Yang ◽  
Kangcheng Wu ◽  
Peng Gao ◽  
Xiaojuan Liu ◽  
Guangpu Li ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cold Stress ◽  
Stress Tolerance ◽  
Glycine Soja ◽  
Leucine Rich Repeat ◽  
Positive Regulator

scholarly journals The rice annexin gene OsAnn5 is a positive regulator of cold stress tolerance at the seedling stage

2020 ◽  
Author(s):  
chunxiu shen ◽  
Zhiqun Que ◽  
Qineng Lu ◽  
Tao Liu ◽  
Shengqiang Li ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Fluorescent Protein ◽  
Functional Characterization ◽  
Survival Rates ◽  
Seedling Stage ◽  
Positive Regulator ◽  
Annexin Gene ◽  
Green Fluorescent

Abstract Annexins exist widely in plants as multigene families and play critical roles in stress responses and a range of cellular processes. In this study, we report on the cloning and functional characterization of the rice annexin gene OsAnn5. We found that the expression of OsAnn5 was induced by cold stress treatment at the seedling stage of rice. GUS staining assay indicated that the expression of OsAnn5 was non tissue-specific and was detected in almost all rice tissues. Subcellular localization indicated that OsAnn5-GFP (green fluorescent protein) signals were found in the endoplasmic reticulum apparatus. Compared with wild type rice, overexpression of OsAnn5 significantly increased survival rates at the seedling stage under cold stress, while knocking out OsAnn5 using the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR associated proteins) mediated genome editing resulted in sensitivity to cold treatments. These results indicate that OsAnn5 is a positive regulator of cold stress tolerance at the seedling stage.

scholarly journals Genotype and Trait Specific Responses to Rapamycin Intake in Drosophila melanogaster

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 474
Author(s):  
Palle Duun Rohde ◽  
Asbjørn Bøcker ◽  
Caroline Amalie Bastholm Jensen ◽  
Anne Louise Bergstrøm ◽  
Morten Ib Juul Madsen ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Stress ◽  
Side Effects ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Treated Group ◽  
Model System ◽  
Treatment Efficiency ◽  
Evolutionarily Conserved ◽  
Heat Stress Tolerance

Rapamycin is a powerful inhibitor of the TOR (Target of Rapamycin) pathway, which is an evolutionarily conserved protein kinase, that plays a central role in plants and animals. Rapamycin is used globally as an immunosuppressant and as an anti-aging medicine. Despite widespread use, treatment efficiency varies considerably across patients, and little is known about potential side effects. Here we seek to investigate the effects of rapamycin by using Drosophila melanogaster as model system. Six isogenic D. melanogaster lines were assessed for their fecundity, male longevity and male heat stress tolerance with or without rapamycin treatment. The results showed increased longevity and heat stress tolerance for male flies treated with rapamycin. Conversely, the fecundity of rapamycin-exposed individuals was lower than for flies from the non-treated group, suggesting unwanted side effects of the drug in D. melanogaster. We found strong evidence for genotype-by-treatment interactions suggesting that a ‘one size fits all’ approach when it comes to treatment with rapamycin is not recommendable. The beneficial responses to rapamycin exposure for stress tolerance and longevity are in agreement with previous findings, however, the unexpected effects on reproduction are worrying and need further investigation and question common believes that rapamycin constitutes a harmless drug.

scholarly journals Myo-inositol as a main metabolite in overwintering flies: seasonal metabolomic profiles and cold stress tolerance in a northern drosophilid fly

2012 ◽  
Vol 215 (16) ◽  
pp. 2891-2897 ◽  
Author(s):  
L. Vesala ◽  
T. S. Salminen ◽  
V. Kostal ◽  
H. Zahradnickova ◽  
A. Hoikkala
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Main Metabolite

scholarly journals Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

2018 ◽  
Vol 13 (1) ◽  
pp. 431-445 ◽  
Author(s):  
Wei Tang ◽  
Caroline Luo
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Salt Stress Tolerance ◽  
Dependent Protein Kinase ◽  
Zinc Finger Transcription Factor ◽  
Transgenic Cell ◽  
Dependent Protein

AbstractThe purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of Arabidopsis thaliana 6 (ZAT6) in salt stress tolerance in cells of rice (Oryza sativa L.), cotton (Gossypium hirsutum L.) and slash pine (Pinus elliottii Engelm.). Cells of O. sativa, G. hirsutum, and P. elliottii overexpressing ZAT6 were generated using Agrobacterium-mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of ZAT6 increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA8, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, ZAT6 also increased expression of Ca2+-dependent protein kinase genes OsCPK9 and OsCPK25 by 5–7 fold under NaCl stress. Altogether, our results suggest that overexpression of ZAT6 enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca2+-dependent protein kinase in transgenic cell lines of different plant species.

scholarly journals Molecular breeding of water lily: engineering cold stress tolerance into tropical water lily

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Cuiwei Yu ◽  
Guirong Qiao ◽  
Wenmin Qiu ◽  
Dongbei Yu ◽  
Shirong Zhou ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Molecular Breeding ◽  
Water Lily ◽  
Tropical Water
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