scholarly journals Protein kinase and ribonuclease domains of IRE1 confer stress tolerance, vegetative growth, and reproductive development in Arabidopsis

2013 ◽  
Vol 110 (48) ◽  
pp. 19633-19638 ◽  
Author(s):  
Y. Deng ◽  
R. Srivastava ◽  
S. H. Howell
Keyword(s):  
Protein Kinase ◽  
Stress Tolerance ◽  
Vegetative Growth ◽  
Reproductive Development

scholarly journals Genotype and Trait Specific Responses to Rapamycin Intake in Drosophila melanogaster

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 474
Author(s):  
Palle Duun Rohde ◽  
Asbjørn Bøcker ◽  
Caroline Amalie Bastholm Jensen ◽  
Anne Louise Bergstrøm ◽  
Morten Ib Juul Madsen ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Stress ◽  
Side Effects ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Treated Group ◽  
Model System ◽  
Treatment Efficiency ◽  
Evolutionarily Conserved ◽  
Heat Stress Tolerance

Rapamycin is a powerful inhibitor of the TOR (Target of Rapamycin) pathway, which is an evolutionarily conserved protein kinase, that plays a central role in plants and animals. Rapamycin is used globally as an immunosuppressant and as an anti-aging medicine. Despite widespread use, treatment efficiency varies considerably across patients, and little is known about potential side effects. Here we seek to investigate the effects of rapamycin by using Drosophila melanogaster as model system. Six isogenic D. melanogaster lines were assessed for their fecundity, male longevity and male heat stress tolerance with or without rapamycin treatment. The results showed increased longevity and heat stress tolerance for male flies treated with rapamycin. Conversely, the fecundity of rapamycin-exposed individuals was lower than for flies from the non-treated group, suggesting unwanted side effects of the drug in D. melanogaster. We found strong evidence for genotype-by-treatment interactions suggesting that a ‘one size fits all’ approach when it comes to treatment with rapamycin is not recommendable. The beneficial responses to rapamycin exposure for stress tolerance and longevity are in agreement with previous findings, however, the unexpected effects on reproduction are worrying and need further investigation and question common believes that rapamycin constitutes a harmless drug.

scholarly journals Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

2018 ◽  
Vol 13 (1) ◽  
pp. 431-445 ◽  
Author(s):  
Wei Tang ◽  
Caroline Luo
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Salt Stress Tolerance ◽  
Dependent Protein Kinase ◽  
Zinc Finger Transcription Factor ◽  
Transgenic Cell ◽  
Dependent Protein

AbstractThe purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of Arabidopsis thaliana 6 (ZAT6) in salt stress tolerance in cells of rice (Oryza sativa L.), cotton (Gossypium hirsutum L.) and slash pine (Pinus elliottii Engelm.). Cells of O. sativa, G. hirsutum, and P. elliottii overexpressing ZAT6 were generated using Agrobacterium-mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of ZAT6 increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA8, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, ZAT6 also increased expression of Ca2+-dependent protein kinase genes OsCPK9 and OsCPK25 by 5–7 fold under NaCl stress. Altogether, our results suggest that overexpression of ZAT6 enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca2+-dependent protein kinase in transgenic cell lines of different plant species.

Abstract P214: The Non-canonical Stress Tolerance Cascade for Toll-like Receptor 9 in the Vasculature Signals Through Liver Kinase B1

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Cameron G McCarthy ◽  
Camilla F Wenceslau ◽  
Safia Ogbi ◽  
Theodora Szasz ◽  
R.Clinton Webb
Keyword(s):  
Protein Kinase ◽  
Stress Tolerance ◽  
Vascular Function ◽  
Cytosolic Calcium ◽  
Toll Like Receptor ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Liver Kinase B1 ◽  
Tlr9 Activation

Toll-like receptor (TLR)9 is a pattern recognition receptor of the innate immune system. Recently, a non-canonical stress tolerance pathway has been reported for TLR9 in non-immune cells (cardiomyocytes and neurons), independent of inflammatory signaling. It was observed that TLR9 inhibited sarco/endoplasmic reticulum Ca 2+ -ATPase (SERCA)2, increasing cytosolic calcium, and resulting in 5’ AMP-activated protein kinase (AMPK)α activation. In our laboratory, we have reported that TLR9 treatment in vivo causes arterial dysfunction that contributes to the pathogenesis of hypertension and that these phenotypes occurred in conjunction with vascular AMPKα phosphorylation (Thr172). However, whether a dysregulation in calcium homeostasis via the non-canonical stress tolerance cascade underlies the impaired vascular function after TLR9 stimulation needs to be clarified. We hypothesized that TLR9 activation would inhibit SERCA2 activity in the vasculature. SERCA2 activity was assessed using a luciferase-based ATP quantification kit. Microsomes were isolated from pooled aortae of Sprague-Dawley rats and subjected to treatment with either Vehicle (Veh) or ODN2395 (2 μM), with or without a SERCA2 inhibitor (thapsigargin; 1 μM). The presence of thapsigargin increased ATP concentrations similarly in both Veh and ODN2395 [ATP (μM), Veh: 19±3 vs. Veh+thapsigargin: 140±35; ODN2395: 22±9 vs. ODN2395+thapsigargin: 129±12, both p<0.05], suggesting TLR9 activation did not inhibit SERCA2 activity. Next, MRA from Sprague-Dawley rats were divided into three sections for Western blot analysis of AMPKα-activating kinases, specifically calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2) and liver kinase B1 (LKB1). ODN2395 alone did not increase protein expression of phospho-CaMKK2 Ser511 (p>0.05), again suggesting calcium-independent activation of AMPKα. However, ODN2395 did increase phospho-LKB1 Ser428 (3.8 fold vs. Veh, p<0.05), and this increase in expression was inhibited by pre-incubation with TLR9 antagonist ODN2088 (20 μM) (p>0.05). These results suggest that the TLR9 non-canonical stress tolerance pathway in the vasculature is mediated by LKB1, and not SERCA2 inhibition.

Response of soya beans to planting date in south-eastern Queensland. II.* Vegetative and reproductive development

1974 ◽  
Vol 25 (5) ◽  
pp. 723 ◽  
Author(s):  
RJ Lawn ◽  
DE Byth
Keyword(s):  
Seed Yield ◽  
Vegetative Growth ◽  
Growth Period ◽  
Reproductive Development ◽  
Planting Date ◽  
Biological Efficiency ◽  
Vegetative Development ◽  
Planting Dates ◽  
Growing Period ◽  
Seed Yields

Vegetative and reproductive development of a range of soya bean cultivars was studied over a series of planting dates in both hill plots and row culture at Redland Bay, Qld. Responses in the extent of vegetative and reproductive development were related to changes in the phasic developmental patterns. The duration and extent of vegetative development for the various cultivar-planting date combinations were closely associated with the length of the period from planting to the cessation of flowering. Thus, vegetative growth was greatest for those planting dates which resulted in a delay in flowering and/or extended the flowering phase. Similarly, genetic lateness of maturity among cultivars was associated with more extensive vegetative development. Seed yield per unit area increased within each cultivar as the length of the growing period was extended until sufficient vegetative growth occurred to allow the formation of closed canopies under the particular agronomic conditions imposed. Further increases in the length of the period of vegetative growth failed to increase seed yield, and in some cases seed yields were actually reduced. Biological efficiency of seed production (BE) was negatively correlated with the length of the vegetative growth period. Differences in BE among cultivar-planting date combinations were large. It is suggested that maximization of seed yield will necessitate an optimum compromise between the degree of vegetative development and BE. Optimum plant arrangement will therefore vary, depending on the particular cultivar-planting date combination. ___________________ \*Part I, Aust. J. Agric. Res., 24: 67 (1973).

Effects of Limb Girdling on Growth and Development of Competing Fruit and Vegetative Tissues of Peach Trees

1984 ◽  
Vol 11 (2) ◽  
pp. 49 ◽  
Author(s):  
IR Dann ◽  
RA Wildes ◽  
DJ Chalmers
Keyword(s):  
Growth Regulators ◽  
Vegetative Growth ◽  
Aging Process ◽  
Prunus Persica ◽  
Soluble Sugars ◽  
Reproductive Development ◽  
Reproductive Growth ◽  
Potential Growth ◽  
Vegetative Tissues ◽  
Peach Trees

The distribution of current assimilates between competing zones of potential growth in the peach tree (Prunus persica (L.) Batsch) was studied using limb girdling, which altered the balance between reproductive growth and vegetative growth in a similar manner to the aging process. Fruit matured earlier, and leaf senescence and abscission were advanced in girdled limbs. which supported normal fruit loads but had only half the leaf area. Lateral growth and secondary thickening were reduced by 50% but vegetative growth approached normal rates at times when fruit growth was minimal, indicating that girdling reduced the ability of vegetative growth to compete with reproductive growth for assimilates. Starch and soluble sugars did not accumulate above the girdles. The data are consistent with the hypothesis that girdling alters the balance between endogenous growth regulators which favour either vegetative or reproductive development. We suggest that the initial effects on the girdled limb are attributable to accumulation of growth regulators produced above the girdle. The reduced flow of growth regulators to the roots eventually results in lowered levels of root-produced hormones which subsequently causes effects throughout the tree.

Effects of Photoperiod on Reproductive Development in Velvetleaf (Abutilon theophrasti)

Weed Science ◽  
1995 ◽  
Vol 43 (4) ◽  
pp. 627-633 ◽  
Author(s):  
David T. Patterson
Keyword(s):  
Weed Management ◽  
Vegetative Growth ◽  
Competitive Ability ◽  
Simulation Models ◽  
Reproductive Development ◽  
Fruit Weight ◽  
Weed Growth ◽  
Growth Differences ◽  
Shoot Weight ◽  
Reciprocal Transfer

When velvetleaf plants from Mississippi and Minnesota populations were maintained in growth chambers with day/night temperatures of 29/23 C and photoperiods of 11, 12, 13, 14, and 15 h, flower buds and open flowers appeared first in the 12 h photoperiod. Buds and flowers appeared 2 to 4 d later at photoperiods of 11, 13, or 14 h. Increasing the photoperiod beyond 14 h to 15 h delayed bud appearance an additional 7 d in the MN plants and 12 d in the MS plants. Open flowers appeared four to five nodes higher, 10 d later at 15 than at 14 h in the MN plants and 20 d later in the MS plants. Vegetative shoot weight and fruit weight 73 d after emergence were greater in 13 h or longer photoperiods than at 11 or 12 h. In shorter photoperiods, MN plants produced more vegetative growth than MS plants, but the reverse occurred at longer photoperiods where MS plants were taller than MN plants. These growth differences occurred because earlier shifts in allocation to reproductive growth in MN plants limited their vegetative growth, particularly in the longest photoperiods. Differences in rate of reproductive development between populations were not evident until photoperiod exceeded 13 h. Reciprocal transfer of plants of the MS population between short and long photoperiods revealed the durations of the juvenile (pre-inductive), inductive, and post-inductive phases to be 3 to 5, 7 to 8 (short day) or 30 (long day), and 10 to 11 d, respectively. Differences in competitive ability among latitudinal biotypes of photoperiodically-sensitive weeds may depend on time of emergence in the field and consequent photoperiod exposure. Weed growth simulation models to be used in development of expert systems for weed management should take photoperiodic sensitivity into account.

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