Knockdown of MicroRNA160a/b by STTM leads to root architecture changes via auxin signaling in Solanum tuberosum

2021 ◽  
Author(s):  
Jiangwei Yang ◽  
Ning Zhang ◽  
Jinlin Zhang ◽  
Xin Jin ◽  
Xi Zhu ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Root Architecture ◽  
Auxin Signaling

scholarly journals Overexpression of the Auxin Receptor AFB3 in Arabidopsis Results in Salt Stress Resistance and the Modulation of NAC4 and SZF1

2020 ◽  
Vol 21 (24) ◽  
pp. 9528
Author(s):  
Fernanda Garrido-Vargas ◽  
Tamara Godoy ◽  
Ricardo Tejos ◽  
José Antonio O’Brien
Keyword(s):  
Salt Stress ◽  
Stress Response ◽  
Growth And Development ◽  
Root Architecture ◽  
Crop Production ◽  
Lateral Roots ◽  
Auxin Signaling ◽  
Auxin Receptor ◽  
Salt Stress Response ◽  
Regulatory Pathways

Soil salinity is a key problem for crop production worldwide. High salt concentration in soil negatively modulates plant growth and development. In roots, salinity affects the growth and development of both primary and lateral roots. The phytohormone auxin regulates various developmental processes during the plant’s life cycle, including several aspects of root architecture. Auxin signaling involves the perception by specialized receptors which module several regulatory pathways. Despite their redundancy, previous studies have shown that their functions can also be context-specific depending on tissue, developmental or environmental cues. Here we show that the over-expression of Auxin Signaling F-Box 3 receptor results in an increased resistance to salinity in terms of root architecture and germination. We also studied possible downstream signaling components to further characterize the role of auxin in response to salt stress. We identify the transcription factor SZF1 as a key component in auxin-dependent salt stress response through the regulation of NAC4. These results give lights of an auxin-dependent mechanism that leads to the modulation of root system architecture in response to salt identifying a hormonal cascade important for stress response.

scholarly journals Endogenous Hypoxia in Lateral Root Primordia Controls Root Architecture by Antagonizing Auxin Signaling in Arabidopsis

2019 ◽  
Vol 12 (4) ◽  
pp. 538-551 ◽  
Author(s):  
Vinay Shukla ◽  
Lara Lombardi ◽  
Sergio Iacopino ◽  
Ales Pencik ◽  
Ondrej Novak ◽  
...  
Keyword(s):  
Lateral Root ◽  
Root Architecture ◽  
Auxin Signaling ◽  
Root Primordia ◽  
Lateral Root Primordia

scholarly journals Overexpression of OsPT8 Increases Auxin Content and Enhances Tolerance to High-Temperature Stress in Nicotiana tabacum

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 809 ◽  
Author(s):  
Song ◽  
Fan ◽  
Jiao ◽  
Liu ◽  
Wang ◽  
...  
Keyword(s):  
High Temperature ◽  
Temperature Stress ◽  
Root Architecture ◽  
Molecular Mechanisms ◽  
Extreme Temperature ◽  
High Temperature Stress ◽  
Wild Plants ◽  
Auxin Signaling ◽  
Plant Tolerance ◽  
Auxin Content

Temperature is a primary factor affecting the rate of plant development; as the climate warms, extreme temperature events are likely to increasingly affect agriculture. Understanding how to improve crop tolerance to heat stress is a key concern. Wild plants have evolved numerous strategies to tolerate environmental conditions, notably the regulation of root architecture by phytohormones, but the molecular mechanisms of stress resistance are unclear. In this study, we showed that high temperatures could significantly reduce tobacco biomass and change its root architecture, probably through changes in auxin content and distribution. Overexpression of the OsPT8 phosphate transporter enhanced tobacco tolerance to high-temperature stress by changing the root architecture and increased the antioxidant ability. Molecular assays suggested that overexpression of OsPT8 in tobacco significantly increased the expression of auxin synthesis genes NtYUCCA 6, 8 and auxin efflux carriers genes NtPIN 1,2 under high-temperature stress. We also found that the expression levels of auxin response factors NtARF1 and NtARF2 were increased in OsPT8 transgenic tobacco under high-temperature stress, suggesting that OsPT8 regulates auxin signaling in response to high-temperature conditions. Our findings provided new insights into the molecular mechanisms of plant stress signaling and showed that OsPT8 plays a key role in regulating plant tolerance to stress conditions.

scholarly journals Enhanced Properties of Chitosan Microparticles over Bulk Chitosan on the Modulation of the Auxin Signaling Pathway with Beneficial Impacts on Root Architecture in Plants

2019 ◽  
Vol 67 (25) ◽  
pp. 6911-6920 ◽  
Author(s):  
María José Iglesias ◽  
Silvana Lorena Colman ◽  
María Cecilia Terrile ◽  
Ramiro París ◽  
Sergio Martín-Saldaña ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Root Architecture ◽  
Auxin Signaling ◽  
Chitosan Microparticles ◽  
Auxin Signaling Pathway ◽  
Enhanced Properties

scholarly journals Host auxin machinery is important in mediating root architecture changes induced by Pseudomonas fluorescens GM30 and Serendipita indica

2020 ◽  
Author(s):  
Anna Matthiadis ◽  
Poornima Sukumar ◽  
Alyssa DeLeon ◽  
Dale Pelletier ◽  
Jessy L. Labbé ◽  
...  
Keyword(s):  
Plant Growth ◽  
Lateral Root ◽  
Root Architecture ◽  
Fungal Species ◽  
Root Production ◽  
Auxin Signaling ◽  
Beneficial Microbes ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractAuxin is a key phytohormone that is integral to plant developmental processes including those underlying root initiation, elongation, and branching. Beneficial microbes have been shown to have an impact on root development, potentially mediated through auxin. In this study, we explore the role of host auxin signaling and transport components in mediating the root growth promoting effects of beneficial microbes. Towards this end, we undertook co-culture studies of Arabidopsis thaliana plants with microbes previously reported to promote lateral root proliferation and produce auxin. Two types of beneficial microbes were included in the present study; a plant growth promoting bacterial species of interest, Pseudomonas fluorescens GM30, and a well-studied plant growth promoting fungal species, Serendipita indica (Piriformospora indica). Following co-culture, lateral root production was found restored in auxin transport inhibitor-treated plants, suggesting involvement of microbe and/or microbially-produced auxin in altering plant auxin levels. In order to clarify the role of host auxin signaling and transport pathways in mediating interactions with bacterial and fungal species, we employed a suite of auxin genetic mutants as hosts in co-culture screens. Our result show that the transport proteins PIN2, PIN3, and PIN7 and the signaling protein ARF19, are required for mediating root architecture effects by the bacterial and/or fungal species. Mutants corresponding to these proteins did not significantly respond to co-culture treatment and did not show increases in lateral root production and lateral root density. These results implicate the importance of host auxin signaling in both bacterial and fungal induced changes in root architecture and serve as a driver for future research on understanding the role of auxin-dependent and auxin-independent pathways in mediating plant-microbe interactions in economically important crop species.

scholarly journals Trichoderma spp. Improve Growth of Arabidopsis Seedlings Under Salt Stress Through Enhanced Root Development, Osmolite Production, and Na+ Elimination Through Root Exudates

2014 ◽  
Vol 27 (6) ◽  
pp. 503-514 ◽  
Author(s):  
Hexon Angel Contreras-Cornejo ◽  
Lourdes Macías-Rodríguez ◽  
Ruth Alfaro-Cuevas ◽  
José López-Bucio
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Root Exudates ◽  
Root Development ◽  
Root Architecture ◽  
Critical Role ◽  
Auxin Signaling ◽  
Dependent Manner ◽  
Trichoderma Spp

Salt stress is an important constraint to world agriculture. Here, we report on the potential of Trichoderma virens and T. atroviride to induce tolerance to salt in Arabidopsis seedlings. We first characterized the effect of several salt concentrations on shoot biomass production and root architecture of Arabidopsis seedlings. We found that salt repressed plant growth and root development in a dose-dependent manner by blocking auxin signaling. Analysis of the wild type and eir1, aux1-7, arf7arf19, and tir1abf2abf19 auxin-related mutants revealed a key role for indole-3-acetic acid (IAA) signaling in mediating salt tolerance. We also found that T. virens (Tv29.8) and T. atroviride (IMI 206040) promoted plant growth in both normal and saline conditions, which was related to the induction of lateral roots and root hairs through auxin signaling. Arabidopsis seedlings grown under saline conditions inoculated with Trichoderma spp. showed increased levels of abscissic acid, L-proline, and ascorbic acid, and enhanced elimination of Na+ through root exudates. Our data show the critical role of auxin signaling and root architecture to salt tolerance in Arabidopsis and suggest that these fungi may enhance the plant IAA level as well as the antioxidant and osmoprotective status of plants under salt stress.

scholarly journals MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems

2018 ◽  
Vol 96 (5) ◽  
pp. 895-909 ◽  
Author(s):  
Javier Raya-González ◽  
Araceli Oropeza-Aburto ◽  
Jesús S. López-Bucio ◽  
Ángel A. Guevara-García ◽  
Lieven de Veylder ◽  
...  
Keyword(s):  
Cell Viability ◽  
Root Architecture ◽  
Critical Factor ◽  
Auxin Signaling ◽  
Arabidopsis Root ◽  
Root Meristems

Situación económica y social de la población de San José del Carmen, San Cristóbal de las Casas, Chiapas

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
Pedro Cadena-Iñiguez ◽  
Eileen Salinas-Cruz ◽  
Jesús Martínez-Sánchez ◽  
Mariano Morales-Guerra ◽  
Romualdo Vásquez-Ortiz ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Theobroma Cacao ◽  
Zea Mays L ◽  
Solanum Tuberosum L ◽  
San Jose ◽  
Phaseolus Vulgaris L ◽  
Las Casas ◽  
Vicia Faba L ◽  
Las Mujeres ◽  
Theobroma Cacao L

Objetivo: Establecer una línea base como fundamento para la intervención e inducción de innovaciones a través de escuelas de campo y planes de negocios. Diseño/metodología/aproximación: El estudio se desarrolló en San José del Carmen, San Cristóbal de Las Casas, Chiapas, México. Se elaboró una encuesta como instrumento para recabar en campo información de familias de la localidad con la finalidad de conocer su situación actual. La localidad de trabajo se eligió utilizando como criterio las poblaciones que se encuentran dentro de Cruzada Nacional contra el hambre y en la clasificación de la pobreza extrema Resultados: La localidad de San José del Carmen es considerada como de alta marginación, la población es bilingüe, su principal lengua es el Tzotzil, el nivel de estudios promedio es hasta el tercer año de primaria, las principales actividades productivas son la siembra de maíz (Zea mays L.), frijol (Phaseolus vulgaris L.), papa (Solanum tuberosum L.) y haba (Vicia faba L.) que son utilizadas principalmente para autoconsumo.  No cuentan con agua potable ya que su sistema es a través de la recaudación de agua de lluvia o a través de un jagüey. Sus principales actividades económicas son la albañilería por parte de los hombres, y venta de productos, tales como el pozol (bebida energética a base de maíz y cacao (Theobroma cacao L.), tostadas y algunos vegetales por parte de las mujeres. Las tostadas son producidas en forma artesanal con un proceso de doble nixtamalización y vendida en bolsas de 20 tostadas cada una, a un costo de MX$10.00 en los mercados de San Cristóbal de las Casas, una a dos veces por semana alrededor de 40 bolsas por día de venta. Limitaciones del estudio/implicaciones: La afiliación zapatista de alguno de los pobladores y el hecho de que algunos habitantes no hablen español, fueron factores que limitaron desarrollar al 100% el estudio; sin embargo, se considera que se tienen los elementos necesarios para establecer la línea base de la situación de San José del Carmen. Conclusiones: El diagnóstico servirá como una herramienta para establecer una intervención a través de innovaciones y planes de negocios en la comunidad de estudio. Los pobladores cuentan con herramientas necesarias para obtener nuevos conocimientos que ayuden a un desarrollo de su producción. El idioma no deberá de ser una limitante para ello.

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