Antifungal activity and mechanism of action of tannic acid against Penicillium digitatum

2019 ◽  
Vol 107 ◽  
pp. 46-50 ◽  
Author(s):  
Congyi Zhu ◽  
Mengying Lei ◽  
Mebeaselassie Andargie ◽  
Jiwu Zeng ◽  
Jianxiong Li
Keyword(s):  
Antifungal Activity ◽  
Tannic Acid ◽  
Mechanism Of Action ◽  
Penicillium Digitatum

scholarly journals Objective: Antifungal activity of total ethanolic extract and fractions of Usnea varieties were proved against Aspergillus niger, Penicillium digitatum, and Rhizopus nigricans fungi. Methods: To perform the study of relative antifungal activity (AFR), flu

2020 ◽  
pp. 171-174
Author(s):  
DAYANNA CABRA GACHA ◽  
LUIS POMBO OSPINA ◽  
JANETH ARIAS PALACIOS ◽  
OSCAR RODRÍGUEZ AGUIRRE
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Penicillium Digitatum ◽  
Total Extract ◽  
Rhizopus Nigricans ◽  
A Value

Objective: Antifungal activity of total ethanolic extract and fractions of Usnea varieties were proved against Aspergillus niger, Penicillium digitatum, and Rhizopus nigricans fungi. Methods: To perform the study of relative antifungal activity (AFR), fluconazole and ketoconazole were used as standards. The inhibition coefficient 50 (IC50) for each extract and the fractions was determined. Results: It was established that the highest activity was presented by the acetone fraction with a value of 58.53. In the study of antifungal activity, the fraction that presented the best activity against P. digitatum was ethanolic with a value of 44.33, while for R. nigricans was petrol extract, with a value of 75.35 and finally for A. niger was the total extract with a value of 35.48. Conclusions: Comparing the values obtained from the extract and the different fractions resulted in the dichloromethane fraction showing the best values.

Synthesis, in vitro antifungal activity and mechanism of action of four sterol hydrazone analogues against the dimorphic fungus Paracoccidioides brasiliensis

Steroids ◽  
2011 ◽  
Vol 76 (10-11) ◽  
pp. 1069-1081 ◽  
Author(s):  
Gonzalo Visbal ◽  
Gioconda San-Blas ◽  
Alexis Maldonado ◽  
Álvaro Álvarez-Aular ◽  
Mario V. Capparelli ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Mechanism Of Action ◽  
Paracoccidioides Brasiliensis ◽  
Dimorphic Fungus ◽  
In Vitro Antifungal Activity

Antifungal Activity of D0870 against Murine Infections and Its Mechanism of Action

Chemotherapy ◽  
1998 ◽  
Vol 44 (2) ◽  
pp. 112-120 ◽  
Author(s):  
Hidehiko Yamada ◽  
Toshihiko Tsuda ◽  
Taiji Watanabe ◽  
Satoshi Kusakabe ◽  
Hidenori Mochizuki
Keyword(s):  
Antifungal Activity ◽  
Mechanism Of Action ◽  
Murine Infections

Nano-emulsion of denak (Oliveria decumbens Vent.) essential oil: ultrasonic synthesis and antifungal activity against Penicillium digitatum

2021 ◽  
Author(s):  
Somayeh Rafiee ◽  
Asghar Ramezanian ◽  
Reza Mostowfizadeh-Ghalamfarsa ◽  
Mehrdad Niakousari ◽  
Mohammad Jamal Saharkhiz ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Essential Oil ◽  
Penicillium Digitatum ◽  
Ultrasonic Synthesis ◽  
Nano Emulsion

scholarly journals Inhibition Profiling of Human Carbonic Anhydrase II by High-Throughput Screening of Structurally Diverse, Biologically Active Compounds

2006 ◽  
Vol 11 (7) ◽  
pp. 782-791 ◽  
Author(s):  
Rema Iyer ◽  
Albert A. Barrese ◽  
Shilpa Parakh ◽  
Christian N. Parker ◽  
Brian C. Tripp
Keyword(s):  
Carbonic Anhydrase ◽  
Small Molecules ◽  
Tannic Acid ◽  
High Throughput ◽  
Mechanism Of Action ◽  
High Throughput Screening ◽  
Biologically Active ◽  
Carbonic Anhydrase Ii ◽  
Hit Rate ◽  
Human Carbonic Anhydrase

Human carbonic anhydrase II (CA II), a zinc metalloenzyme, was screened against 960 structurally diverse, biologically active small molecules. The assay monitored CA II esterase activity against the substrate 4-nitrophenyl acetate in a format allowing high-throughput screening. The assay proved to be robust and reproducible with a hit rate of ∼2%. Potential hits were further characterized by determining their IC50 and Kd values and tested for nonspecific, promiscuous inhibition. Three known sulfonamide CA inhibitors were identified: acetazolamide, methazolamide, and celecoxib. Other hits were also found, including diuretics and antibiotics not previously identified as CA inhibitors, for example, furosemide and halazone. These results confirm that many sulfonamide drugs have CA inhibitory properties but also that not all sulfonamides are CA inhibitors. Thus many, but not all, sulfonamide drugs appear to interact with CA II and may target other CA isozymes. The screen also yielded several novel classes of nonsulfonamide inhibitors, including merbromin, thioxolone, and tannic acid. Although these compounds may function by some nonspecific mechanism (merbromin and tannic acid), at least 1 (thioxolone) appears to represent a genuine CA inhibitor. Thus, this study yielded a number of potentially new classes of CA inhibitors and preliminary experiments to characterize their mechanism of action.

scholarly journals Potential of some bacteria for biological control of postharvest citrus green mould caused by Penicillium digitatum

2017 ◽  
Vol 53 (No. 3) ◽  
pp. 134-143 ◽  
Author(s):  
Mohammadi Parisa ◽  
Tozlu Elif ◽  
Kotan Recep ◽  
Kotan Merve Şenol
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Mycelial Growth ◽  
Spore Production ◽  
Penicillium Digitatum ◽  
Broth Culture ◽  
Agrobacterium Radiobacter ◽  
Green Mould

Ten bacteria isolate (4 Bacillus subtilis, 2 Bacillus pumilus, 2 Bacillus cereus, 1 Bacillus megaterium, and 1 Agrobacterium radiobacter) were tested in vitro for antagonistic properties against Penicillium digitatum, the causal agent of citrus green mould. The effect of these bacteria was also observed on mycelial growth, spore germination, and spore production of the pathogenic fungus in broth culture. Extracellular enzyme activities of the bacteria were determined. According to the results of in vitro antagonistic tests and enzymes activities, the most promising bacteria were Bacillus subtilis and Agrobacterium radiobacter. These bacteria were tested for disease suppression on lemon fruits. In addition, these bacterial isolates also showed remarkable antifungal activity against the pathogen on lemon fruits. The results of this study showed that Bacillus subtilis and Agrobacterium radiobacter showed remarkable antifungal activity against the pathogen. Chitinase and glucanase enzyme activity of all the tested bacteria was positive. Protease enzyme activity was positive in all tested bacteria with the exception of Agrobacterium radiobacter. In addition, all bacteria inhibited mycelial growth and spore germination (except Agrobacterium radiobacter) of the fungus. Bacillus subtilis, Bacillus cereus, and Agrobacterium radiobacter inhibited spore production in broth culture. Bacillus subtilis and Agrobacterium radiobacter were tested on lemon fruits significantly reduced disease severity. Consequently, these isolates can be used as new biocontrol agents in controlling the post-harvest decay of citrus fruits caused by Penicillium digitatum.

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