Prevalence and characteristics of multidrug-resistant Proteus mirabilis from broiler farms in Shandong Province, China

NDM-1-producing multidrug-resistant Proteus mirabilis brings formidable clinical challenges. We report a nosocomial outbreak of carbapenem-resistant P. mirabilis in China. Six P. mirabilis strains collected in the same ward showed close phylogenetic relatedness, indicating clonal expansion. Illumina and MinION sequencing revealed that three isolates harbored a novel Salmonella genomic island 1 carrying a blaNDM–1 gene (SGI1-1NDM), while three other isolates showed elevated carbapenem resistance and carried a similar SGI1 but with two blaNDM–1 gene copies (SGI1-2NDM). Four new single nucleotide mutations were present in the genomes of the two-blaNDM–1-harboring isolates, indicating later emergence of the SGI1-2NDM structure. Passage experiments indicated that both SGI variants were stably persistent in this clone without blaNDM–1 copy number changes. This study characterizes two novel blaNDM–1-harboring SGI1 variants in P. mirabilis and provides a new insight into resistance gene copy number variation in bacteria.

Download Full-text

Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid

Microorganisms ◽

10.3390/microorganisms8111775 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1775

Author(s):

Andrey Shelenkov ◽

Lyudmila Petrova ◽

Valeria Fomina ◽

Mikhail Zamyatin ◽

Yulia Mikhaylova ◽

...

Keyword(s):

Whole Genome Sequencing ◽

Genome Sequencing ◽

Proteus Mirabilis ◽

Intestinal Microflora ◽

Virulence Genes ◽

Multidrug Resistant ◽

Whole Genome ◽

Long Read ◽

Tract Infections ◽

Eskape Pathogens

Proteus mirabilis is a component of the normal intestinal microflora of humans and animals, but can cause urinary tract infections and even sepsis in hospital settings. In recent years, the number of multidrug-resistant P. mirabilis isolates, including the ones producing extended-spectrum β-lactamases (ESBLs), is increasing worldwide. However, the number of investigations dedicated to this species, especially, whole-genome sequencing, is much lower in comparison to the members of the ESKAPE pathogens group. This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing P. mirabilis isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between Klebsiella pneumoniae and P. mirabilis plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes. We believe that the data presented will contribute to the understanding of antibiotic resistance acquisition and virulence mechanisms for this important pathogen.

Download Full-text

Genome Sequences of Two Multidrug-Resistant Proteus mirabilis Strains Harboring CTX-M-65 Isolated from Malaysia

Genome Announcements ◽

10.1128/genomea.01301-16 ◽

2016 ◽

Vol 4 (6) ◽

Author(s):

Choo Yee Yu ◽

Geik Yong Ang ◽

Yun Fong Ngeow ◽

Kok Keng Tee ◽

Wai-Fong Yin ◽

...

Keyword(s):

Urinary Tract ◽

Urinary Tract Infections ◽

Proteus Mirabilis ◽

Draft Genome ◽

Multidrug Resistant ◽

Urine Samples ◽

Nosocomial Pathogen ◽

Genome Sequences ◽

Encoding Gene ◽

Tract Infections

Proteus mirabilis is an opportunistic nosocomial pathogen that is commonly associated with urinary tract infections. Here, we present draft genome sequences of two multidrug-resistant P. mirabilis strains, isolated from urine samples in Malaysia, that harbored a CTX-M-type extended-spectrum β-lactamase-encoding gene, as well as a repertoire of other antimicrobial-resistant determinants.

Download Full-text

First Report of an OXA-48-Producing Multidrug-Resistant Proteus mirabilis Strain from Gaza, Palestine

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00565-15 ◽

2015 ◽

Vol 59 (7) ◽

pp. 4305-4307 ◽

Cited By ~ 28

Author(s):

Liang Chen ◽

Nahed Al Laham ◽

Kalyan D. Chavda ◽

Jose R. Mediavilla ◽

Michael R. Jacobs ◽

...

Keyword(s):

Resistance Genes ◽

Proteus Mirabilis ◽

Draft Genome ◽

Multidrug Resistant ◽

Aminoglycoside Resistance ◽

Content Type ◽

Antimicrobial Resistance Genes ◽

Carbapenemase Gene ◽

Genes Encoding ◽

Draft Genome Sequencing

ABSTRACTWe report the first multidrug-resistantProteus mirabilisstrain producing the carbapenemase OXA-48 (Pm-OXA-48) isolated at Al-Shifa hospital in Gaza, Palestine. Draft genome sequencing of Pm-OXA-48 identified 16 antimicrobial resistance genes, encoding resistance to β-lactams, aminoglycosides, fluoroquinolones, phenicols, streptothricin, tetracycline, and trimethoprim-sulfamethoxazole. Complete sequencing of theblaOXA-48-harboring plasmid revealed that it is a 72 kb long IncL/M plasmid, harboring carbapenemase geneblaOXA-48, extended spectrum β-lactamase geneblaCTX-M-14, and aminoglycoside resistance genesstrA,strB, andaph(3′)-VIb.

Download Full-text

KARAKTERISTIK ISOLAT Proteus mirabilis PADA SPESIMEN URIN DI RSUP SANGLAH SELAMA TAHUN 2018 – 2019

JURNAL KEDOKTERAN ◽

10.36679/kedokteran.v6i2.364 ◽

2021 ◽

Vol 6 (2) ◽

pp. 121

Author(s):

I Nyoman Arnatha ◽

Komang Januartha Putra Pinatih ◽

Ni Luh Ranthi Kurniawathi

Keyword(s):

Urinary Tract ◽

Blood Urea Nitrogen ◽

Proteus Mirabilis ◽

Multidrug Resistant ◽

Urea Nitrogen ◽

Multidrug Resistant Organisms ◽

Urinary Tract Stones ◽

The Mean ◽

Tract Infections ◽

Urine Specimens

Proteus mirabilis merupakan salah satu bakteri penyebab utama infeksi saluran kemih yang dapat memicu pembentukan kristal atau batu saluran kemih (BSK). Pemberian antibiotik yang adekuat dapat menurunkan resiko pembentukan BSK. Tujuan penelitian ini untuk menentukan karakteristik Proteus mirabilis yang diisolasi dari spesimen urin dan pola kepekaannya terhadap antibiotik. Penelitian ini merupakan penelitian deskriptif yang dilakukan secara potong lintang dengan kurun waktu 2 tahun (1 Januari 2018 sampai 31 Desember 2019) di RS Sanglah, Denpasar, Bali. Semua kultur urin dengan Proteus mirabilis dimasukkan dalam penelitian. Identifikasi bakteri dan sensitivitas terhadap antibiotik dilakukan dengan menggunakan mesin Vitek®2 Compact (BioMerieux). Dari 4.094 spesimen urin yang dikultur, didapatkan 49 isolat Proteus mirabilis dengan karakteristik pasiennya adalah : 49% laki-laki dan 51% perempuan. Usia mayoritas adalah 18-65 tahun (77.6%). Isolat yang menunjukkan adanya BSK adalah 13 isolat (26.5%) dengan lokasi batu paling banyak berada di ginjal sejumlah 9 isolat (69.2%). Rerata jumlah leukosit adalah 13.88 x103/mL (± 7.56). Rerata persentase neutrofil adalah 67.24 % (± 25.38). Rerata kadar Blood Urea Nitrogen (BUN) adalah 27.25 mg/dL (± 26.66). Rerata kadar serum kreatinin adalah 2.64 mg/dL (± 9.15). Persentase Multidrug Resistant Organisms (MDRO) sejumlah 38.8%. Hasil sensitivitas Proteus mirabilis terhadap amikacin sebesar 100%. Penelitian menunjukkan bahwa bakteri Proteus mirabilis cenderung mampu untuk membentuk kristal / BSK. Marker infeksi, fungsi ginjal pasien, dan sensitivitas terhadap antibiotik, tidak menunjukkan perbedaan yang signifikan antara isolat dengan BSK dan tanpa BSK.Kata Kunci : Proteus mirabilis, batu saluran kemih, MDRO ABSTRACTProteus mirabilis is one of the main causes of urinary tract infections which can lead to formation of urinary tract stones. Adequate antibiotics can reduce the risk of stone formation. The aim of this study was to determine characteristics of Proteus mirabilis isolated from urine specimens and its sensitivity to antibiotics. This was a cross-sectional descriptive study with a period of 2 years (1 January 2017 to 31 December 2018) in Sanglah Hospital, Denpasar, Bali. All urine cultures with Proteus mirabilis were included in this study. Identification and sensitivity test were conducted using Vitek-2 Compact machine (BioMerieux). Out of 4,094 urine specimens were cultured, in which 49 isolates of Proteus mirabilis were obtained. Characteristics of patients as follows 49% male and 51% female. The majority of the age is 18-65 years (77.6%). Isolates that showed the presence of stone were 13 isolates (26.5%) with location of the stones were mostly in the kidneys (9 isolates/69.2%). The mean leukocyte count was 13.88 x103/mL (± 7.56). The average percentage of neutrophils was 67.24% (± 25.38). The mean Blood Urea Nitrogen levels was 27.25 mg/dL (± 26.66). The mean serum creatinine level was 2.64 mg/dL (± 9.15). Percentage of Multidrug Resistant Organisms was 38.8%. All of isolates were sensitive to amikacin (100%). This study showed that Proteus mirabilis might be as a cause of urinary tract stone. Infection markers, patient kidney function, and sensitivity to antibiotics did not show a significant difference between isolates with and without stones.Keywords: Proteus mirabilis, urinary tract stones, MDRO

Download Full-text