Behavioral improvements in a valproic acid rat model of autism following vitamin D supplementation

2017 ◽  
Vol 253 ◽  
pp. 28-32 ◽  
Author(s):  
Lin Du ◽  
Guolian Zhao ◽  
Zipeng Duan ◽  
Fan Li
Keyword(s):  
Vitamin D ◽  
Valproic Acid ◽  
Rat Model ◽  
Vitamin D Supplementation

scholarly journals Assessment of Vitamin D Supplementation on Articular Cartilage Morphology in a Young Healthy Sedentary Rat Model

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1260 ◽  
Author(s):  
Marta Anna Szychlinska ◽  
Rosa Imbesi ◽  
Paola Castrogiovanni ◽  
Claudia Guglielmino ◽  
Silvia Ravalli ◽  
...  
Keyword(s):  
Vitamin D ◽  
Articular Cartilage ◽  
Rat Model ◽  
Musculoskeletal Diseases ◽  
Vitamin D Supplementation ◽  
Cartilage Thickness ◽  
Male Rats ◽  
Sprague Dawley ◽  
Cartilage Development ◽  
Joint Lubrication

Deficiency in vitamin D (Vit D) has been widely associated with several musculoskeletal diseases. However, the effects of the exogenous Vit D supplementation are still unclear in the prevention of the latter, especially in the cartilage developmental period. The aim of this study was to compare the effects of Vit D supplementation and restriction on the articular cartilage development in healthy young sedentary rats. To this aim, twelve nine-week-old healthy Sprague–Dawley male rats were subjected to Vit D-based experimental diets: R, with a content in Vit D of 1400 IU/kg; R-DS, with a Vit D supplementation (4000 IU/kg); R-DR, with a Vit D restriction (0 IU/kg) for 10 weeks. The morphology, thickness and expression of cartilage-associated molecules such as collagen type II/X, lubricin and Vit D receptor (VDR), were assessed. Histological, histomorphometric and immunohistochemical evaluations were made on rat tibial cartilage samples. In the present experimental model, restriction of Vit D intake induced: The lower thickness of cartilage compared both to R (p = < 0.0001) and R-DS (p = < 0.0001); reduction of proteoglycans in the extracellular matrix (ECM) compared both to R (p = 0.0359) and R-DS (p = < 0.0001); decreased collagen II (Col II) with respect both to R (p = 0.0076) and R-DS (p = 0.0016); increased collagen X (Col X) immunoexpression when compared both to R (p = < 0.0001) and R-DS (p = < 0.0001), confirming data from the literature. Instead, supplementation of Vit D intake induced: Higher cartilage thickness with respect both to R (p = 0.0071) and R-DR (p = < 0.0001); increase of ECM proteoglycan deposition compared both to R (p = 0.0175) and R-DR (p = < 0.0001); higher immunoexpression of lubricin with respect both to R (p = 0.001) and R-DR (p = 0.0008). These results suggest that Vit D supplementation with diet, already after 10 weeks, has a favorable impact on the articular cartilage thickness development, joint lubrication and ECM fibers deposition in a young healthy rat model.

scholarly journals Effects of Vitamin D Supplementation during the Induction and Progression of Osteoarthritis in a Rat Model

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
E. C. Castillo ◽  
M. A. Hernandez-Cueto ◽  
M. A. Vega-Lopez ◽  
C. Lavalle ◽  
J. B. Kouri ◽  
...  
Keyword(s):  
Vitamin D ◽  
Protective Effect ◽  
Rat Model ◽  
Genetic Regulation ◽  
Cartilage Degradation ◽  
Epidemiological Studies ◽  
Vitamin D Supplementation ◽  
Toll Like Receptor ◽  
Chronic Stage ◽  
Low Levels

Epidemiological studies correlate low levels of vitamin D with the osteoarthritis (OA) progression. Cytokines and metalloproteases play a major role in OA promoting the inflammation and degradation of the cartilage and can be induced through the Toll-like receptor (TLR) pathway. The aim of this study was to evaluate the protective effect of vitamin D supplementation on the development of osteoarthritis (OA) through examining the genetic regulation of TLRs, cytokines, and metalloproteases in chondrocytes as well as the wideness of cartilage in rats with OA. Our results demonstrate that the signaling through TLR-4 is a proinflammatory mechanism in osteoarthritis that drives the upregulation of MMP-3, IL-1β, and TNF-αgene expression, leading to cartilage degradation and inflammation. Vitamin D supplementation had a protective effect during the onset but not during the chronic stage of OA in the rat model.

scholarly journals Reversal of Haemostatic Changes in Letrozole-Induced PCOS Rat Model with Vitamin D Supplementation

2019 ◽  
Vol 87 (June) ◽  
pp. 1753-1767
Author(s):  
SUZAN M.M. MOURSI, M.D. EMAN EL-BAHAIE, Ph.D. ◽  
SHEREIN F. EL-SAYED, M.D.
Keyword(s):  
Vitamin D ◽  
Rat Model ◽  
Vitamin D Supplementation

scholarly journals Effect of Vitamin D Status on Vascular Function of the Aorta in a Rat Model of PCOS

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
K. Lajtai ◽  
R. Tarszabó ◽  
B. Bányai ◽  
B. Péterffy ◽  
D. Gerszi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Rat Model ◽  
Vascular Function ◽  
Polycystic Ovary ◽  
Vascular Dysfunction ◽  
Vitamin D Supplementation ◽  
Female Rats ◽  
Vitamin D Status ◽  
Nitrative Stress ◽  
Contraction And Relaxation

Polycystic ovary syndrome (PCOS) is associated with elevated cardiovascular risk. Early vascular dysfunction may lead to the development of cardiovascular disease in PCOS. Vitamin D deficiency (VDD) is a common comorbidity of PCOS that contributes to the pathogenesis of the disease and its complications. Both PCOS and VDD are accompanied by increased oxidative stress that may be involved in the arising vascular dysfunction. We aimed to investigate the role of vitamin D status on aortic function. PCOS was induced by an 8-week-long transdermal testosterone treatment of female rats, and low and adequate vitamin D status was achieved by dietary means. Contraction and relaxation abilities of isolated aortic segments were measured by myograph. Resorcin-fuchsin staining and immunohistochemical labeling of 3-nitrotyrosine were performed. No difference was shown in the norepinephrine-induced contraction of the aortas of different groups, whereas we detected reduced acetylcholine- and insulin-evoked relaxation in VDD groups. A lower level of resorcin-fuchsin staining and elevated 3-nitrotyrosine immunostaining was observed in VDD. In our study, we demonstrated early endothelial dysfunction in VDD PCOS rat model. Vitamin D supplementation could prevent vascular disturbances, while VDD itself damaged endothelium-dependent vasorelaxation and induced nitrative stress.

Vitamin D supplementation and bone markers in ambulatory children on long-term valproic acid therapy. A prospective interventional study

2019 ◽  
Vol 97 ◽  
pp. 192-196
Author(s):  
Margarita Papassava ◽  
Iliada Nakou ◽  
Ekaterini Siomou ◽  
Vasileios Cholevas ◽  
Anna Challa ◽  
...  
Keyword(s):  
Vitamin D ◽  
Valproic Acid ◽  
Bone Markers ◽  
Vitamin D Supplementation ◽  
Interventional Study ◽  
Acid Therapy ◽  
Valproic Acid Therapy

scholarly journals Vitamin D supplementation improves pathophysiology in a rat model of preeclampsia

2016 ◽  
Vol 310 (4) ◽  
pp. R346-R354 ◽  
Author(s):  
Jessica L. Faulkner ◽  
Denise C. Cornelius ◽  
Lorena M. Amaral ◽  
Ashlyn C. Harmon ◽  
Mark W. Cunningham ◽  
...  
Keyword(s):  
Vitamin D ◽  
T Cells ◽  
Rat Model ◽  
Cd4 T Cells ◽  
Vitamin D Supplementation ◽  
Hypertensive Disorder ◽  
Treatment Of Hypertension ◽  
Hypertensive Disorder Of Pregnancy ◽  
Uterine Perfusion ◽  
Placental Ischemia

Deficiency of vitamin D (VD) is associated with preeclampsia (PE), a hypertensive disorder of pregnancy characterized by proinflammatory immune activation. We sought to determine whether VD supplementation would reduce the pathophysiology and hypertension associated with the reduced uterine perfusion pressure (RUPP) rat model of PE. Normal pregnant (NP) and RUPP rats were supplemented with VD2 or VD3 (270 IU and 15 IU/day, respectively) on gestation days 14–18 and mean arterial pressures (MAPs) measured on day 19. MAP increased in RUPP to 123 ± 2 mmHg compared with 102 ± 3 mmHg in NP and decreased to 113 ± 3 mmHg with VD2 and 115 ± 3 mmHg with VD3 in RUPP rats. Circulating CD4+ T cells increased in RUPP to 7.90 ± 1.36% lymphocytes compared with 2.04 ± 0.67% in NP but was lowered to 0.90 ± 0.19% with VD2 and 4.26 ± 1.55% with VD3 in RUPP rats. AT1-AA, measured by chronotropic assay, decreased from 19.5 ± 0.4 bpm in RUPPs to 8.3 ± 0.5 bpm with VD2 and to 15.4 ± 0.7 bpm with VD3. Renal cortex endothelin-1 (ET-1) expression was increased in RUPP rats (11.6 ± 2.1-fold change from NP) and decreased with both VD2 (3.3 ± 1.1-fold) and VD3 (3.1 ± 0.6-fold) supplementation in RUPP rats. Plasma-soluble FMS-like tyrosine kinase-1 (sFlt-1) was also reduced to 74.2 ± 6.6 pg/ml in VD2-treated and 91.0 ± 16.1 pg/ml in VD3-treated RUPP rats compared with 132.7 ± 19.9 pg/ml in RUPP rats. VD treatment reduced CD4+ T cells, AT1-AA, ET-1, sFlt-1, and blood pressure in the RUPP rat model of PE and could be an avenue to improve treatment of hypertension in response to placental ischemia.

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