Elucidating the key factors in semicontinuous anaerobic digestion of urban biowaste: The crucial role of sludge addition in process stability, microbial community enrichment and methane production

2021 ◽  
Author(s):  
B. Tonanzi ◽  
A. Gallipoli ◽  
A. Gianico ◽  
D. Montecchio ◽  
P. Pagliaccia ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Crucial Role ◽  
Process Stability ◽  
Key Factors

Effect of biochar addition on microbial community and methane production during anaerobic digestion of food wastes: The role of minerals in biochar

2021 ◽  
Vol 323 ◽  
pp. 124585
Author(s):  
Yusron Sugiarto ◽  
Nimas Mayang S. Sunyoto ◽  
Mingming Zhu ◽  
Isabelle Jones ◽  
Dongke Zhang
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Food Wastes

scholarly journals The feedstock microbiome selectively steers process stability during the anaerobic digestion of waste activated sludge

2019 ◽  
Author(s):  
Cindy Ka Y Law ◽  
Rens De Henau ◽  
Jo De Vrieze
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Methane Production ◽  
Cost Effective ◽  
Organic Loading Rate ◽  
Waste Activated Sludge ◽  
Process Performance ◽  
Process Stability ◽  
Process Disturbances

AbstractStrategies to enhance process performance of anaerobic digestion remain of key importance to achieve further spreading of this technology for integrated resource recovery from organic waste streams. Continuous inoculation of the microbial community in the digester via the feedstock could be such a cost-effective strategy. Here, anaerobic digestion of fresh waste activated sludge (WAS) was compared with sterilized WAS in response to two common process disturbances, i.e., organic overloading and increasing levels of salts, to determine the importance of feedstock inoculation. A pulse in the organic loading rate severely impacted process performance of the digesters fed sterile WAS, with a 92 ± 45 % decrease in methane production, compared to a 42 ± 31 % increase in the digesters fed fresh WAS, relative to methane production before the pulse. Increasing salt pulses did not show a clear difference in process performance between the digesters fed fresh and sterile WAS, and process recovery was obtained even at the highest salt pulse of 25 g Na+ L−1. Feedstock sterilisation strongly impacted the microbial community in the digesters. In conclusion, feedstock inoculation can be considered a cheap, yet, disturbance-specific strategy to enhance process stability in full-scale anaerobic digestion processes.

Active and total microbial community dynamics and the role of functional genes bamA and mcrA during anaerobic digestion of phenol and p-cresol

2018 ◽  
Vol 264 ◽  
pp. 290-297 ◽  
Author(s):  
Oscar Franchi ◽  
Patricia Bovio ◽  
Eduardo Ortega-Martínez ◽  
Francisca Rosenkranz ◽  
Rolando Chamy
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Community Dynamics ◽  
Functional Genes ◽  
Microbial Community Dynamics

scholarly journals Thermophilic Alkaline Fermentation Followed by Mesophilic Anaerobic Digestion for Efficient Hydrogen and Methane Production from Waste-Activated Sludge: Dynamics of Bacterial Pathogens as Revealed by the Combination of Metagenomic and Quantitative PCR Analyses

2018 ◽  
Vol 84 (6) ◽  
Author(s):  
Jingjing Wan ◽  
Yuhang Jing ◽  
Yue Rao ◽  
Shicheng Zhang ◽  
Gang Luo
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Quantitative Pcr ◽  
Methane Production ◽  
Bacterial Pathogens ◽  
Methane Yield ◽  
Metagenomic Analysis ◽  
Waste Activated Sludge ◽  
Hydrogen Yield

ABSTRACT Thermophilic alkaline fermentation followed by mesophilic anaerobic digestion (TM) for hydrogen and methane production from waste-activated sludge (WAS) was investigated. The TM process was also compared to a process with mesophilic alkaline fermentation followed by a mesophilic anaerobic digestion (MM) and one-stage mesophilic anaerobic digestion (M) process. The results showed that both hydrogen yield (74.5 ml H 2 /g volatile solids [VS]) and methane yield (150.7 ml CH 4 /g VS) in the TM process were higher than those (6.7 ml H 2 /g VS and 127.8 ml CH 4 /g VS, respectively) in the MM process. The lowest methane yield (101.2 ml CH 4 /g VS) was obtained with the M process. Taxonomic results obtained from metagenomic analysis showed that different microbial community compositions were established in the hydrogen reactors of the TM and MM processes, which also significantly changed the microbial community compositions in the following methane reactors compared to that with the M process. The dynamics of bacterial pathogens were also evaluated. For the TM process, the reduced diversity and total abundance of bacterial pathogens in WAS were observed in the hydrogen reactor and were further reduced in the methane reactor, as revealed by metagenomic analysis. The results also showed not all bacterial pathogens were reduced in the reactors. For example, Collinsella aerofaciens was enriched in the hydrogen reactor, which was also confirmed by quantitative PCR (qPCR) analysis. The study further showed that qPCR was more sensitive for detecting bacterial pathogens than metagenomic analysis. Although there were some differences in the relative abundances of bacterial pathogens calculated by metagenomic and qPCR approaches, both approaches demonstrated that the TM process was more efficient for the removal of bacterial pathogens than the MM and M processes. IMPORTANCE This study developed an efficient process for bioenergy (H 2 and CH 4 ) production from WAS and elucidates the dynamics of bacterial pathogens in the process, which is important for the utilization and safe application of WAS. The study also made an attempt to combine metagenomic and qPCR analyses to reveal the dynamics of bacterial pathogens in anaerobic processes, which could overcome the limitations of each method and provide new insights regarding bacterial pathogens in environmental samples.

Effect of solid-state NaOH pretreatment on methane production from thermophilic semi-dry anaerobic digestion of rose stalk

2016 ◽  
Vol 73 (12) ◽  
pp. 2913-2920 ◽  
Author(s):  
Yue-Gan Liang ◽  
Beijiu Cheng ◽  
You-Bin Si ◽  
De-Ju Cao ◽  
Dao-Lin Li ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Solid State ◽  
Methane Production ◽  
Treated Group ◽  
Net Energy ◽  
Process Stability ◽  
Rosa Rugosa ◽  
Naoh Pretreatment ◽  
Volatile Solids ◽  
Dry Anaerobic Digestion

Abstract The effects of solid-state NaOH pretreatment on the efficiency of methane production from semi-dry anaerobic digestion of rose (Rosa rugosa) stalk were investigated at various NaOH loadings (0, 1, 2, and 4% (w/w)). Methane production, process stability and energy balance were analyzed. Results showed that solid-state NaOH pretreatment significantly improved biogas and methane yields of 30-day anaerobic digestion, with increases from 143.7 mL/g volatile solids (VS) added to 157.1 mL/g VS –192.1 mL/g VS added and from 81.8 mL/g VS added to 88.8 mL/g VS–117.7 mL/g VS added, respectively. Solid-state NaOH pretreatment resulted in anaerobic digestion with higher VS reduction and lower technical digestion time. The 4% NaOH-treated group had the highest methane yield of 117.7 mL/g VS added, which was 144% higher compared to the no NaOH-treated group, and the highest net energy recovery. Higher rate of lignocellulose breakage and higher process stability of anaerobic digestion facilitated methane production in the NaOH-pretreated groups.

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